Q681term | arm-PA; Q681term | arm-PB; Q681term | arm-PC; Q681term | arm-PD; Q681term | arm-PE; Q681term | arm-PF
Nucleotide substitution: ?3850?.
Mutation resulting in the truncation of the protein after amino acid 680.
Nucleotide substitution: C3850T.
adult cuticle & abdomen | somatic clone
The segment polarity phenotype and the lack of naked cuticle between denticle belts, resulting in a lawn of denticles on the ventral epidermis, characteristic for maternally and zygotically mutant arm2 embryos is not modified by Scer\GAL4arm.PU-driven expression of any of the following: sggScer\UAS.T:Myr-Src64B,T:Ivir\HA1, sggKK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1 or sggScer\UAS.T:Ivir\HA1.
The increase in average cell number in the stalks between egg chambers which is seen in Mmp2Y53N/Mmp2W307stop females after being shifted to the restrictive temperature is dominantly suppressed if the females also carry a single copy of arm2.
Approximately 90% of arm2; p130CAS1 flies exhibit smaller cuticles than arm2 mutants. Among these, 82% complete lack cuticle in the head region, and 65% have enhanced posterior curvature suggesting a strong germband retraction phenotype. While 40% of arm2 ; p130CAS1 flies retain the lawn of denticles characteristic of an arm2 homozygous mutant, 34% exhibit complete deletion of ventral and dorsal denticle belts, while 36% have well-separated posterior denticle belts.
Embryos triple mutant for sggM11 both maternally and zygotically and arm2 both maternally and zygotically and l(2)gl4 zygotically show a cuticle phenotype that is similar to that of zygotic l(2)gl4 single mutants.
Embryos expressing DadScer\UAS.cTa under the control of Scer\GAL4btl.PS in an arm2 background show outgrowth of the dorsal branches, cell intercalation and autocellular adherens junction formation in the tracheal system.
Expression of salmScer\UAS.cdCa under the control of Scer\GAL4btl.PS partially rescues the lack of dorsal trunk (DT) seen in the tracheal system of arm2 embryos. DT cells are able to migrate anteriorly and posteriorly in these rescued embryos, but the branches do not fuse. The formation of all tracheal branches except the transverse connective and visceral branch is impaired in arm2 ; tkv8 double mutants.
The segment polarity cuticle phenotype of embryos derived from homozygous arm2 female germline clones is partially rescued by Mmus\Catnbarm.PW and not rescued by Hsap\JUParm.PW, when the transgene is provided maternally. The phenotype of these embryos is also partially rescued by Mmus\CatnbScer\UAS.cWa expressed under the control of Scer\GAL4arm.PS and is not rescued by Hsap\JUPScer\UAS.cWa expressed under the control of Scer\GAL4arm.PS.
The "lawn of denticles" cuticle phenotype seen in arm2 embryos is substantially rescued by ubiquitous expression of armScer\UAS.T:sev-TM using Scer\GAL4da.G32 (stripes of naked cuticle are restored). Ubiquitous expression of armS10.Scer\UAS.T:Hsap\MYC in embryos using Scer\GAL4da.G32 produces ectopic naked cuticle in the arm2 background.
The segment polarity phenotype of embryos maternally and zygotically mutant for arm2 is substantially rescued by armScer\UAS.T:Hsap\MYC,T:Ocun\nes or armScer\UAS.T:Hsap\MYC,T:SV40\nls2 expressed under the control of Scer\GAL4e22c.
Homozygous clones in the eye that are weakly rescued with armBCD7 may show ectopic differentiation of retinal tissue. Many clones are associated with polarity inversions; the clones frequently show polarity inversions on their equatorial side. The polarity inversions are usually contained within the clone, but clones in dorsal regions of the eye show clear non-autonomy, with up to two to four ommatidial rows distant from the clone showing chirality changes.
The segment polarity cuticle phenotype of embryos derived from homozygous female germline clones is rescued to wild-type by arm+mWa, when the transgene is provided maternally. The phenotype of these embryos is also rescued by armScer\UAS.cWa expressed under the control of Scer\GAL4arm.PS and partially rescued by armΔC.Scer\UAS expressed under the control of Scer\GAL4arm.PS.