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General Information
Symbol
Dmel\arm8
Species
D. melanogaster
Name
FlyBase ID
FBal0000716
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
armH8.6
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Mutation resulting in the truncation of the protein after amino acid 725.

P element insertion into sixth exon between nucleotides 3985--3986.

Insertion components
P{}arm8
Expression Data
Reporter Expression
Additional Information
Statement
Reference

arm8 protein shows wild type subcellular localization.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The epidermis of arm8 mutant embryos lacks the wide expanse of naked cuticle separating the denticle belts. Most of these embryos fail to hatch.

arm8 embryos show normal dorsal closure. arm8/+ eyes are normal in appearance.

arm8 somatic stem cell (SSC) clones in the ovary are lost slightly more rapidly than wild-type clones, but are lost at a slower rate than arm2 clones. arm8 follicle cell clones have normal morphology compared to neighbouring wild-type follicle cells.

arm2 has no dramatic effect on germline stem cell (GSC) maintenance in the ovary; approximately 60% of mutant GSCs observed in ovaries during the first week after clone induction are maintained for two more weeks (compared to 70% for controls).

Mutant embryos have segment polarity defects, although head involution and dorsal closure are normal.

arm8 embryos show a similar tracheal branching phenotype to arm4 embryos.

Homozygous clones in the wing show no disturbance in planar polarity.

Homozygous clones in eyes show normal ommatidial polarity.

arm8 embryos derived from homozygous female germ line clones (lacking both zygotic and maternal arm function) lack RP2 neurons. They show a broad array of central nervous system defects, including gaps in the longitudinals and poorly formed commissures.

Embryos incubated at the restrictive temperature after acridine orange injection show increased cell death in a similar pattern to that seen for wgl-12 embryos shifted at stage 11. Cell death in the rest of the segment is similar to wild type.

Homozygous clones in the eye are extremely small and only infrequently observed.

Temperature-sensitive allele. Homozygous clones are recovered at reasonable frequency in the wing pouch of discs shifted to the restrictive temperature 18 hours before the end of the third larval instar stage, but not in discs shifted to the restrictive temperature 48 hours before the end of the third larval instar stage. Homozygous clones are produced in the part of the disc that gives rise to the dorsal thorax under both conditions.

Embryos that are zygotically and maternally mutant for arm8 have a very strong segment polarity phenotype, and secrete only denticles with no naked cuticle. Embryos that are zygotically and maternally mutant for arm8 and are expressing armS10.Scer\UAS.T:Hsap\MYC (using Scer\GAL4e22c) essentially secrete only naked cuticle.

Embryos exhibit severe segment polarity defects.

Dorsal tracheal trunks exhibit irregular width and the connections between lateral trunks is incomplete.

When arm8; armBCD7/+ flies are shifted to 25oC during the third larval instar the resulting adults have missing pattern elements along the wing margin and alula. The abdomens lack sternites.

Embryos lacking both maternal and zygotic sgg and arm function have an embryonic cuticle phenotype like that of arm mutant embryos alone. Embryos lacking maternal sgg and arm but receiving wild type paternal copies develop into normal embryos that survive until adulthood. Embryos lacking maternal sgg and arm but receiving a wild type paternal copy of arm+ only, show a sgg naked cuticle phenotype.

Clonal analysis reveals that oogenesis is normal, including with respect to cytoplasmic actin distribution, but reduction in maternal contribution enhances the embryonic phenotype. Eggs derived from mutant germlines are laid, and have normal anterior-posterior and dorso-ventral patterning.

Very strong segment polarity phenotype. Homozygous clones survive at a higher frequency in the dorsal regions of the leg disc, this biased distribution is produced by differential survival, not directed migration.

Late third instar larvae have very small imaginal discs, pupae die without differentiating any adult cuticle.

temperature-sensitive allele

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Enhanced by
Statement
Reference

arm8 has lethal | recessive | embryonic stage phenotype, non-enhanceable by Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]/Rac1J10/Rac1[+]

Suppressed by
Statement
Reference

arm8 has lethal | recessive | embryonic stage phenotype, suppressible | partially by tum[+]/tumAR2

arm8 has lethal | recessive | embryonic stage phenotype, suppressible | partially by pav[+]/pavB200

NOT suppressed by
Statement
Reference

arm8 has lethal | recessive | embryonic stage phenotype, non-suppressible by Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]/Rac1J10/Rac1[+]

Enhancer of
Statement
Reference

arm8/arm[+] is an enhancer of partially lethal phenotype of lqfR03685/lqfRΔ117

NOT Enhancer of
Statement
Reference

arm8/arm[+] is a non-enhancer of visible phenotype of Scer\GAL4en-e16E, kermitGS2053

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

arm8/arm[+] is a non-suppressor of visible phenotype of Scer\GAL4en-e16E, kermitGS2053

Other
Phenotype Manifest In
NOT Enhanced by
Statement
Reference

arm8 has abdominal ventral denticle belt | embryonic stage phenotype, non-enhanceable by Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]/Rac1J10/Rac1[+]

arm8 has phenotype, non-enhanceable by Apc2ΔS

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

arm8 has abdominal ventral denticle belt | embryonic stage phenotype, non-suppressible by Rac2Δ/Mtl[+]/MtlΔ/Rac2[+]/Rac1J10/Rac1[+]

arm8 has phenotype, non-suppressible by Apc2ΔS

Enhancer of
Statement
Reference

arm8 is an enhancer of eye phenotype of Src42AKR.hs.2sev

NOT Enhancer of
Statement
Reference

arm8/arm[+] is a non-enhancer of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053

Suppressor of
NOT Suppressor of
Statement
Reference

arm8/arm[+] is a non-suppressor of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053

arm8 is a non-suppressor of phenotype of ApcQ8

arm8 is a non-suppressor of phenotype of dshhs.sev.B

Other
Additional Comments
Genetic Interactions
Statement
Reference

The formation of supernumerary neuroblasts which is seen in clones derived from single type II neuroblasts expressing kluScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4wor.PA is partially suppressed if the clones are also mutant for arm8.

arm8/+ ; lqfR03685/lqfRΔ117 animals die in the pupal case. They have small or absent eyes, small head capsules and also have morphological defects in the head cuticle, wings and legs.

Heterozygosity for either tumAR2 or pavB200 restores areas of naked cuticle between the belts to almost wide-type levels, and many more arm8 mutants are able to hatch into the first instar stage.

Heterozygosity for a Rac1J10 Rac2Δ MtlΔ triple mutant chromosome does not alter arm8 mutant patterning nor its rate of hatching.

p130CAS1 homozygotes in combination with heterozygous arm8 severely reduces viability.

Expression of the Src42AKR.hs.2sev transgene in a arm8 background results in a rough eye phenotype.

arm8/Y ; enaGC1/enaGC1 embryos have strong defects in dorsal closure and head involution, with no change in the segment polarity phenotype compared to arm8/Y embryos.

The arm8 mutant phenotype is not affected if the embryos are also maternally and zygotically homozygous for Apc2ΔS.

Photoreceptors are not rescued from death in ApcQ8 homozygotes carrying one copy of arm8.

Embryos derived from homozygous doubly mutant germ lines, receiving neither maternal or zygotic wild type product, for sggM11 and arm8 the ventral cuticle is covered with denticles.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

There is a phenotypic series in the clonal germline phenotype that parallels the affect in embryos.

arm8; armBCD7/+ is a synthetic temperature sensitive allelic combination: flies are wild type at 17oC and mutant at 25oC.

Encodes a truncated protein that can still be phosphorylated.

Association of arm protein with α-Cat and shg in vivo is assayed by SDS-PAGE and immunoblotting.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (52)