C13457462T
R753term | arr-PA
?753term
Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
The premature stop codon is introduced between EGF-like repeats 2 and 3.
Amino acid replacement: ?753term.
lethal (with Df(2R)BSC274)
Maternally and zygotically mutant arr2 embryos (coming from mothers whose whole germline constitutes from arr2 mutant cells only - created by the OvoD germline clone technique) show a segment polarity phenotype and no naked cuticle between denticle belts on the ventral epidermis (resulting in a lawn of denticles).
Embryos mutant for both maternal and zygotic arr2 show lawns of denticles uninterrupted by naked cuticle.
Somatic clones of arr2 homozygous cells in the wing pouch region of third instar wing discs are smaller on average than their wild-type twin spots (P=1.6x10-7). Mutant wing pouch clones are associated with increased apoptosis marker expression. When these clones fall in the notum region of this disc there is no difference in mutant clone and twin spot size.
Homozygous clones are not recovered in the wing pouch when the clones are induced early, although small mutant clones are detected in the wing pouch and hinge cells.
Clones expressing arr2 that are induced at the anterior margin in the ventral part of the eye have extra ommatidia in the position normally occupied by the pigment rim. arr2 clones can violate the normal margin and grow out into the head region. Such ectopic eye tissue is heavily bristled. However, in clones that respect the normal eye boundary, the only observed phenotype is the presence of bristles adjacent to the head capsule.
Early induced clones do not survive well.
Clones in the wing cause notching with loss of margin bristles.
Homozygous clones in the eye produce two distinct effects that occur at roughly the same frequency. Firstly, homozygous clones induce ectopic differentiation of the retina ahead of the morphogenetic furrow. Secondly, clones that do not induce ectopic differentiation show clear repolarising effects in the equatorial/polar axis, inducing polarity reversals on their equatorial side. At the poles, the clones exert their maximal influence into surrounding wild-type tissue, inverting the polarity over many ommatidial rows. This effect diminishes progressively with the distance of the clone from the pole, disappearing at the equator. Within the clone, the chiralities of the ommatidia are not randomly arranged; in the equatorial region of the clone the ommatidia are inverted into the inappropriate form found in neighbouring wild-type tissue, but in the polar regions of the clone, the ommatidia are of the correct chiral form (the form found polar to the clone).
arr2 has cell growth defective | somatic clone phenotype, enhanceable by pucE69/puc[+]
arr2 has segment polarity phenotype | embryonic stage phenotype, non-enhanceable by sggUAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has segment polarity phenotype | embryonic stage phenotype, non-enhanceable by sggKK-MI.UAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has segment polarity phenotype | embryonic stage phenotype, non-enhanceable by Scer\GAL4da.PU/sggUAS.Tag:HA
arr2 has increased cell death | somatic clone phenotype, suppressible by Scer\GAL4αTub84B.PL/BacA\p35UAS.cHa
arr2 has cell growth defective | somatic clone phenotype, suppressible | partially by pucUAS.cMa/Scer\GAL4αTub84B.PL
arr2 has segment polarity phenotype | embryonic stage phenotype, non-suppressible by sggUAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has segment polarity phenotype | embryonic stage phenotype, non-suppressible by sggKK-MI.UAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has segment polarity phenotype | embryonic stage phenotype, non-suppressible by Scer\GAL4da.PU/sggUAS.Tag:HA
arr2 has visible | recessive | somatic clone phenotype, non-suppressible by fz2UAS.cCa/Scer\GAL4Bx-MS1096
arrk08131/arr2 is a suppressor of neuroanatomy defective | larval stage phenotype of nwk2
arr2 is a suppressor | somatic clone of visible phenotype of wgGMR.PW
arr2, egh[+]/egh62d18 has neuroanatomy defective | larval stage phenotype
arr2 has embryonic/larval cuticle | embryonic stage phenotype, non-enhanceable by sggUAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has denticle field | embryonic stage phenotype, non-enhanceable by sggUAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has embryonic/larval cuticle | embryonic stage phenotype, non-enhanceable by sggKK-MI.UAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has denticle field | embryonic stage phenotype, non-enhanceable by sggKK-MI.UAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has embryonic/larval cuticle | embryonic stage phenotype, non-enhanceable by Scer\GAL4da.PU/sggUAS.Tag:HA
arr2 has denticle field | embryonic stage phenotype, non-enhanceable by Scer\GAL4da.PU/sggUAS.Tag:HA
arr2 has denticle belt | maternal effect phenotype, suppressible | partially by Scer\GAL4prd.RG1/dshUAS.cAa
arr2 has embryonic/first instar larval cuticle | maternal effect phenotype, suppressible | partially by Scer\GAL4prd.RG1/dshUAS.cAa
arr2 has wing disc | somatic clone phenotype, suppressible | partially by Scer\GAL4αTub84B.PL/BacA\p35UAS.cHa
arr2 has embryonic/first instar larval cuticle phenotype, suppressible | partially by Scer\GAL4prd.RG1/arr::fz2arr.intra.UAS.Tag:MYC
arr2 has denticle belt phenotype, suppressible | partially by Scer\GAL4prd.RG1/arr::fz2arr.intra.UAS.Tag:MYC
arr2 has embryonic/larval cuticle | embryonic stage phenotype, non-suppressible by sggUAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has denticle field | embryonic stage phenotype, non-suppressible by sggUAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has embryonic/larval cuticle | embryonic stage phenotype, non-suppressible by sggKK-MI.UAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has denticle field | embryonic stage phenotype, non-suppressible by sggKK-MI.UAS.Tag:Myr(Src64B),Tag:HA/Scer\GAL4da.PU
arr2 has embryonic/larval cuticle | embryonic stage phenotype, non-suppressible by Scer\GAL4da.PU/sggUAS.Tag:HA
arr2 has denticle field | embryonic stage phenotype, non-suppressible by Scer\GAL4da.PU/sggUAS.Tag:HA
arr2 has wing margin bristle | somatic clone phenotype, non-suppressible by fz2UAS.cCa/Scer\GAL4Bx-MS1096
arr2 has wing | somatic clone phenotype, non-suppressible by fz2UAS.cCa/Scer\GAL4Bx-MS1096
arrk08131/arr2 is a suppressor of NMJ bouton | supernumerary | larval stage phenotype of nwk2
arr2 is a suppressor | somatic clone of ommatidium phenotype of wgGMR.PW
arr2 is a non-suppressor of denticle belt phenotype of Scer\GAL4arm.PS, arr::dshDIX-ctail.UAS
arr2 is a non-suppressor of embryonic/first instar larval cuticle phenotype of Scer\GAL4arm.PS, arr::dshDIX-ctail.UAS
arr2, egh[+]/egh62d18 has embryonic/larval neuromuscular junction phenotype
arr2, egh[+]/egh62d18 has terminal bouton | larval stage phenotype
BacA\p35UAS.cHa, Scer\GAL4αTub84B.PL, arr2 has G1 phase | somatic clone phenotype
BacA\p35UAS.cHa, Scer\GAL4αTub84B.PL, arr2 has G2 phase | somatic clone phenotype
The segment polarity phenotype and the lack of naked cuticle between denticle belts, resulting in a lawn of denticles on the ventral epidermis, characteristic for maternally and zygotically mutant arr2 embryos is not modified by Scer\GAL4da.PU-driven expression of any of the following: sggScer\UAS.T:Myr-Src64B,T:Ivir\HA1, sggKK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1, or sggScer\UAS.T:Ivir\HA1.
None of the embryos that are mutant for both maternal and zygotic arr2 and express Scer\GAL4prd.RG1>dshScer\UAS.cAa exhibit broad stripes of excess cuticle. Some of the embryos show occasional small patches of naked cuticle within their denticle lawns.
Expression of dshDIX.Scer\UAS.T:ctail-arr in arr2 mutants under the control of Scer\GAL4arm.PS produces embryos with completely naked cuticles.
Recovery of arr2 homozygous somatic clones in third instar wing discs (induced at around 60 hours after egg laying) is reduced by the presence of pucE69/+ (10/25 twinspots vs 21/29 in a wild-type background).
pucScer\UAS.cMa; Scer\GAL4αTub84B.PL only partially rescues arr2 homozygous somatic clone size in the wing disc: these clones are on average 32% of twin-spot clone size, compared to 17% in the absence of pucScer\UAS.cMa.
Recovery of arr2 M(2)53+ homozygous somatic clones in a M(2)531/+; arr2/+ background is less than arr+; M(2)53+ controls in the same background (clone survival assayed in third instar wing discs; induced at around 60 hours after egg laying).
Recovery of arr2 homozygous somatic clones in third instar wing discs (induced at around 60 hours after egg laying) is reduced by the presence of pucE69/+ (10/25 twinspots vs 21/29 in a wild-type background). pucScer\UAS.cMa; Scer\GAL4αTub84B.PL only partially rescues arr2 homozygous somatic clone size in the wing disc: These clones are on average 32% of twin-spot clone size, compared to 17% in the absence of pucScer\UAS.cMa. BacA\p35Scer\UAS.cHa; Scer\GAL4αTub84B.PL only partially rescues arr2 homozygous somatic clone size in the wing disc: These clones are on average 56% of twin-spot clone size, compared to 17% in the absence of BacA\p35Scer\UAS.cHa. Many mutant clone cells sort out below the disc epithelium. Flow cytometry analysis of these mutant clone cells shows an increase in the fraction of cells in G2 at the expense of cells in G1 compared to wild-type control cells. Recovery of arr2 M(2)53+ homozygous somatic clones in a M(2)531/+; arr2/+ background is less than arr+; M(2)53+ controls in the same background (clone survival assayed in third instar wing discs; induced at around 60 hours after egg laying).
Clone size is substantially increased in arr2 mutant clones in the wing disc if they are also expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4αTub84B.PL.
The periodic character of the cuticles made by sggM11; arm8 maternal/zygotic double mutant embryos is suppressed by arr2/arr2, with the resulting cuticle tending toward denticle cell fates. The loss of naked cuticle in arr2/arr2 embryos is partially suppressed by arr::fz2arr.intra.Scer\UAS.T:Hsap\MYC; Scer\GAL4prd.RG1. Suppression is more robust in more anterior segments.
The notching and loss of margin bristles seen in arr2 clones in the wing is not suppressed if fz2Scer\UAS.cCa is expressed in these flies under the control of Scer\GAL4Bx-MS1096.
BacA\p35Scer\UAS.cHa; Scer\GAL4αTub84B.PL only partially rescues arr2 homozygous somatic clone size in the wing disc: these clones are on average 56% of twin-spot clone size, compared to 17% in the absence of BacA\p35Scer\UAS.cHa. Many mutant clone cells sort out below the disc epithelium. Flow cytometry analysis of these mutant clone cells shows an increase in the fraction of cells in G2 at the expense of cells in G1 compared to wild-type control cells.
arr2 is rescued by Scer\GAL4unspecified/arrUAS.Tag:SS(wg),GFP,Tag:MYC