The haltere to wing partial transformation exhibited by most ash1R1288A/ash122 transheterozygotes is fully suppressed by one copy of Df(2R)XL26 and is partially suppressed by the expression of Caf1-55::MRG15Scer\UAS.cHa or Caf1-55::MRG15Δchromo.Scer\UAS, but not of MRG15Scer\UAS.cHa or Caf1-55Scer\UAS.cHa, under the control of Scer\GAL4arm.PS; their third leg to second leg partial transformation, however, is not suppressed by the expression of Caf1-55::MRG15Scer\UAS.cHa under the control of Scer\GAL4arm.PS.
ash122 sktlΔ15 double heterozygotes show a homeotic transformation of third leg to second leg (3.7% penetrance). ash122 sktlΔ20 double heterozygotes show a homeotic transformation of third leg to second leg (2.4% penetrance).
The frequency of transformation of halteres into wings seen in Dsp11/Y flies is enhanced by ash122/+. The enhancement is dramatically reduced if the female parents are heterozygous rather than homozygous for Dsp11.
Double heterozygotes with Df(2L)GpdhA, Df(2L)cl-h4, Df(2L)cl-h1, Df(2L)E110, Df(2L)Mdh, Df(2R)or-BR6, Df(3L)Ar14-8, Df(2L)cl-h3, Df(2R)Pu-D17, Df(3L)vin6, lidk06801, lid10424, E(Pc)1, E(Pc)2, Su(z)21, Psc1, AsxXF23, Asx3, Asx13, SceD1, E(z)5, TrlR85, Su(z)71, Su(z)61, ScmD1 or ScmM56 show T3 to T2 transformations.
Either ash1ΔSET.Ubi-p63E or ash1ΔPHD.Ubi-p63E homozygosity rescue ash122/Df(3L)Exel9011 transheterozygotes to adulthood. However, adults have low fitness, with no stable stocks being established, and all individuals show homeotic transformation.
ash1R1464A/+ expressing ash122/ash122 animals that contain maternally deposited and zygotically expressed Ash1 protein with the R1464A point mutation exhibit similar homeotic transformations as ash122/ash122 pharate adults. A substantial fraction of them eclose from the pupal case but die in 1-2 days.