Allele Dmel\ash1²²

General Information
Symbol	Dmel\ash1²²	Species	D. melanogaster
Name		FlyBase ID	FBal0000754
Feature type	allele	Associated gene	Dmel\ash1
Also Known As	ash1^VV183

Map ( GBrowse )
Allele class	amorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	amorphic allele - genetic evidence (Tripoulas et al., 1996)
Mutagen	ethyl methanesulfonate (Tripoulas et al., 1994, Tripoulas et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:19,590,887..19,590,887 pr_change=Q121@\|ash1-PA na_change=C19590887T evidence=experimental reported_na_change=C1155T reported_pr_change=Q47@ (Tripoulas et al., 1996)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: C1155T. Amino acid replacement: Q47@. (Tripoulas et al., 1996)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Tripoulas et al., 1996, Tripoulas et al., 1994, Sanchez-Elsner et al., 2006)
Phenotype Manifest In
	haltere disc (Papp and Muller, 2006)
Detailed Description
	Statement Reference Homozygous haltere discs are larger than normal, probably due to a partial transformation into wing discs. (Papp and Muller, 2006) Lethality occurs during the larval and pupal stages. Double heterozygotes with Ubx^abx-1, trx^el2 or brm² exhibit transformation of third leg to second leg identity and haltere to wing transformation. (Tripoulas et al., 1996)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference ash1[+]/ash1²² is an enhancer of visible \| homeotic phenotype of Dsp1¹ (Decoville et al., 2001) ash1²² is an enhancer of eye color defective phenotype of w^{4.Ubx.bxd:pbx.N} (Rozovskaia et al., 1999)
Suppressor of
Statement Reference ash1[+]/ash1²² is a suppressor of visible \| dominant phenotype of gcm^Pyx (Popkova et al., 2012)
Other
Statement Reference +/Df(2L)Mdh, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) ash1²², lid[+]/lid¹⁰⁴²⁴ has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) ash1²², lid¹⁰⁴²⁴ has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) ash1²², lid^k06801/lid[+] has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) ash1²², lid^k06801 has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) ash1²², sktl[+]/sktl^Δ15 has visible \| dominant \| homeotic phenotype (Cheng and Shearn, 2004) ash1²², sktl[+]/sktl^Δ20 has visible \| dominant \| homeotic phenotype (Cheng and Shearn, 2004) ash1²², sktl^Δ15 has visible \| dominant \| homeotic phenotype (Cheng and Shearn, 2004) ash1²², sktl^Δ20 has visible \| dominant \| homeotic phenotype (Cheng and Shearn, 2004) Asx[+]/Asx¹³, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Asx³, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Asx³/Asx[+], ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Asx¹³, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Asx^XF23, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Asx^XF23/Asx[+], ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Df(2L)cl-h1/+, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Df(2L)cl-h3/+, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Df(2L)cl-h4/+, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Df(2L)E110/+, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Df(2L)GpdhA/+, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Df(2R)or-BR6/+, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Df(2R)Pu-D17/+, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Df(3L)Ar14-8/+, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Df(3L)vin6/+, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) E(Pc)[+]/E(Pc)¹, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) E(Pc)[+]/E(Pc)², ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) E(Pc)¹, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) E(Pc)², ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) E(z)[+]/E(z)⁵, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) E(z)⁵, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Psc[+]/Psc¹, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Psc¹, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Sce[+]/Sce^D1, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Sce^D1, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Scm^D1, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Scm^D1/Scm[+], ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Scm^M56, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Scm^M56/Scm[+], ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Su(z)2¹, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Su(z)2¹/Su(z)2[+], ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Su(z)6[+]/Su(z)6¹, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Su(z)6¹, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Su(z)7[+]/Su(z)7¹, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Su(z)7¹, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Trl[+]/Trl^R85, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000) Trl^R85, ash1²² has visible \| dominant \| homeotic phenotype (Gildea et al., 2000)
Phenotype Manifest In
Enhancer of
Statement Reference ash1[+]/ash1²² is an enhancer of adult abdominal segment 4 \| ectopic phenotype of E(z)^Trm (Bajusz et al., 2001) ash1[+]/ash1²² is an enhancer of adult abdominal segment 5 phenotype of E(z)^Trm (Bajusz et al., 2001) ash1[+]/ash1²² is an enhancer of adult mesothoracic segment \| ectopic phenotype of E(z)^Trm (Bajusz et al., 2001) ash1[+]/ash1²² is an enhancer of adult metathoracic segment phenotype of E(z)^Trm (Bajusz et al., 2001) ash1[+]/ash1²² is an enhancer of adult prothoracic segment phenotype of E(z)^Trm (Bajusz et al., 2001) ash1[+]/ash1²² is an enhancer of haltere phenotype of Dsp1¹ (Decoville et al., 2001) ash1[+]/ash1²² is an enhancer of wing \| ectopic phenotype of Dsp1¹ (Decoville et al., 2001) ash1[+]/ash1²² is an enhancer of wing vein \| ectopic phenotype of Snr1^E1 (Marenda et al., 2004) ash1²² is an enhancer of eye phenotype of CycE^JP (Brumby et al., 2002) ash1²² is an enhancer of pigment cell phenotype of w^{4.Ubx.bxd:pbx.N} (Rozovskaia et al., 1999)
NOT Enhancer of
Statement Reference ash1²² is a non-enhancer of eye phenotype of Scer\GAL4^ey.PH, osa^Scer\UAS.cCa (Collins et al., 1999)
Suppressor of
Statement Reference ash1[+]/ash1²² is a suppressor of chaeta \| supernumerary phenotype of gcm^Pyx (Popkova et al., 2012) ash1[+]/ash1²² is a suppressor of wing vein \| ectopic phenotype of Snr1^E1 (Marenda et al., 2004) ash1²² is a suppressor of wing phenotype of Scer\GAL4^OK386, nej^Scer\UAS.cMa (Bantignies et al., 2000)
NOT Suppressor of
Statement Reference ash1²² is a non-suppressor of eye phenotype of Scer\GAL4^ey.PH, osa^Scer\UAS.cCa (Collins et al., 1999)
Other
Statement Reference +/Df(2L)Mdh, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) ash1²², lid[+]/lid¹⁰⁴²⁴ has adult metathoracic segment phenotype (Gildea et al., 2000) ash1²², lid¹⁰⁴²⁴ has adult metathoracic segment phenotype (Gildea et al., 2000) ash1²², lid^k06801/lid[+] has adult metathoracic segment phenotype (Gildea et al., 2000) ash1²², lid^k06801 has adult metathoracic segment phenotype (Gildea et al., 2000) ash1²², sktl[+]/sktl^Δ15 has mesothoracic leg phenotype (Cheng and Shearn, 2004) ash1²², sktl[+]/sktl^Δ15 has metathoracic leg \| ectopic phenotype (Cheng and Shearn, 2004) ash1²², sktl[+]/sktl^Δ20 has mesothoracic leg phenotype (Cheng and Shearn, 2004) ash1²², sktl[+]/sktl^Δ20 has metathoracic leg \| ectopic phenotype (Cheng and Shearn, 2004) ash1²², sktl^Δ15 has mesothoracic leg phenotype (Cheng and Shearn, 2004) ash1²², sktl^Δ15 has metathoracic leg \| ectopic phenotype (Cheng and Shearn, 2004) ash1²², sktl^Δ20 has mesothoracic leg phenotype (Cheng and Shearn, 2004) ash1²², sktl^Δ20 has metathoracic leg \| ectopic phenotype (Cheng and Shearn, 2004) Asx[+]/Asx¹³, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Asx³, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Asx³/Asx[+], ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Asx¹³, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Asx^XF23, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Asx^XF23/Asx[+], ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Df(2L)cl-h1/+, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Df(2L)cl-h3/+, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Df(2L)cl-h4/+, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Df(2L)E110/+, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Df(2L)GpdhA/+, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Df(2R)or-BR6/+, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Df(2R)Pu-D17/+, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Df(3L)Ar14-8/+, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Df(3L)vin6/+, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) E(Pc)[+]/E(Pc)¹, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) E(Pc)[+]/E(Pc)², ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) E(Pc)¹, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) E(Pc)², ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) E(z)[+]/E(z)⁵, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) E(z)⁵, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Psc[+]/Psc¹, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Psc¹, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Sce[+]/Sce^D1, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Sce^D1, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Scm^D1, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Scm^D1/Scm[+], ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Scm^M56, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Scm^M56/Scm[+], ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Su(z)2¹, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Su(z)2¹/Su(z)2[+], ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Su(z)6[+]/Su(z)6¹, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Su(z)6¹, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Su(z)7[+]/Su(z)7¹, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Su(z)7¹, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Trl[+]/Trl^R85, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000) Trl^R85, ash1²² has adult metathoracic segment phenotype (Gildea et al., 2000)
Additional Comments
Genetic Interactions
Statement Reference ash1²² sktl^Δ15 double heterozygotes show a homeotic transformation of third leg to second leg (3.7% penetrance). ash1²² sktl^Δ20 double heterozygotes show a homeotic transformation of third leg to second leg (2.4% penetrance). (Cheng and Shearn, 2004) At 29^oC, ash1²² suppresses the ectopic vein that extends distally from the posterior crossvein in Snr1^E1 animals. but enhances the extra vein material seen posterior of the L5 wing vein. (Marenda et al., 2004) The frequency of transformation of halteres into wings seen in Dsp1¹/Y flies is enhanced by ash1²²/+. The enhancement is dramatically reduced if the female parents are heterozygous rather than homozygous for Dsp1¹. (Decoville et al., 2001) Double heterozygotes with Df(2L)GpdhA, Df(2L)cl-h4, Df(2L)cl-h1, Df(2L)E110, Df(2L)Mdh, Df(2R)or-BR6, Df(3L)Ar14-8, Df(2L)cl-h3, Df(2R)Pu-D17, Df(3L)vin6, lid^k06801, lid¹⁰⁴²⁴, E(Pc)¹, E(Pc)², Su(z)2¹, Psc¹, Asx^XF23, Asx³, Asx¹³, Sce^D1, E(z)⁵, Trl^R85, Su(z)7¹, Su(z)6¹, Scm^D1 or Scm^M56 show T3 to T2 transformations. (Gildea et al., 2000) The addition of ash1²² does not affect the variable eye size phenotype seen in osa^Scer\UAS.cCa/Scer\GAL4^ey.PH flies. (Collins et al., 1999) The eye colour of flies carrying w^{4.Ubx.bxd:pbx.N} is strongly reduced if they are also heterozygous for ash1²². (Rozovskaia et al., 1999)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	24161 w^*; ash1²² P{FRT(w^hs)}2A/TM6C, Sb¹ Tb¹
Notes on Origin
Discoverer

Comments
	Range of lethality for transheterozygotes extends from the third larval instar for the most severe alleles to the pharate adult stage for the least severe alleles. (Tripoulas et al., 1994)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	ash1²² (Papp and Muller, 2006, Beltran et al., 2007, Popkova et al., 2012) Ash1²² (Sanchez-Elsner et al., 2006, Sanchez-Elsner et al., 2006) ash1^VV183 (Cheng and Shearn, 2004, Gildea et al., 2000) ash1^vv183 (Lopez et al., 2001, Decoville et al., 2001) dash²² (Lindsley and Zimm, 1992) dash^VV183 (Shearn et al., 1971) VV183
Name Synonym
Secondary FlyBase IDs

References ( 22 )

Generate a list of	All references relating to this allele ( 22 )

List References by type	Research paper ( 20 ) Supplementary material ( 1 ) Book ( 1 )
Recent research papers ( 1 )
	Popkova et al., 2012, PLoS Genet. 8(12): e1003159 Polycomb controls gliogenesis by regulating the transient expression of the gcm/glide fate determinant. [FBrf0220515] Show all research papers ...

Allele Dmel\ash122

Allele Dmel\ash1²²