Allele Dmel\ash2¹

General Information
Symbol	Dmel\ash2¹	Species	D. melanogaster
Name		FlyBase ID	FBal0000757
Feature type	allele	Associated gene	Dmel\ash2
Also Known As	ash2⁷⁰³

Allele class	amorphic allele - genetic evidence
Mutagen	N-methyl-N'-nitro-N-nitrosoguanidine
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Shearn, 1989, LaJeunesse and Shearn, 1995)
Mutagen	N-methyl-N'-nitro-N-nitrosoguanidine (LaJeunesse and Shearn, 1995, Adamson and Shearn, 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference 3kb insertion. (Adamson and Shearn, 1996)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| larval stage \| recessive lethal \| pupal stage (with ash2¹⁴) (Adamson and Shearn, 1996) lethal \| pupal stage \| recessive (Adamson and Shearn, 1996) lethal \| recessive (LaJeunesse and Shearn, 1995)
Phenotype Manifest In
	antenna (Adamson and Shearn, 1996) genitalia (Adamson and Shearn, 1996) haltere (Adamson and Shearn, 1996) metathoracic leg (Adamson and Shearn, 1996) metathoracic leg (with ash2⁹) (Adamson and Shearn, 1996) proboscis (Adamson and Shearn, 1996) prothoracic leg (Adamson and Shearn, 1996) prothoracic segment \| dorsal (Adamson and Shearn, 1996)
Detailed Description
	Statement Reference Lethality occurs after puparium formation. Mutations elicits a wide range of homeotic transformations: proboscis to leg and/or antenna, antenna to leg, dorsal prothorax to wing, first leg to second leg, haltere to wing, third leg to second leg and genitalia to leg and/or antenna. ash2¹/ash2⁹ transheterozygote third legs display overgrowth and supernumerary leg. (Adamson and Shearn, 1996) Lethality acts during the L2/L3 larval instars.
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference ash2¹⁸/ash2¹ has lethal phenotype, enhanceable by sktl[+]/sktl^Δ15 (Cheng and Shearn, 2004)
NOT Enhancer of
Statement Reference ash2[+]/ash2¹ is a non-enhancer of visible \| homeotic phenotype of Dsp1¹ (Decoville et al., 2001)
Suppressor of
Statement Reference ash2¹ is a suppressor of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, dm^Scer\UAS.cZa (Secombe et al., 2007)
Other
Statement Reference ash2¹⁸/ash2¹, sktl[+]/sktl^Δ15 has developmental rate defective phenotype (Cheng and Shearn, 2004) ash2¹⁸/ash2¹, sktl^Δ15 has developmental rate defective phenotype (Cheng and Shearn, 2004)
Phenotype Manifest In
Enhancer of
Statement Reference ash2¹/ash1¹ is an enhancer of adult abdominal segment 4 \| ectopic phenotype of E(z)^Trm (Bajusz et al., 2001) ash2¹/ash1¹ is an enhancer of adult abdominal segment 5 phenotype of E(z)^Trm (Bajusz et al., 2001) ash2¹/ash1¹ is an enhancer of adult mesothoracic segment \| ectopic phenotype of E(z)^Trm (Bajusz et al., 2001) ash2¹/ash1¹ is an enhancer of adult metathoracic segment phenotype of E(z)^Trm (Bajusz et al., 2001) ash2¹/ash1¹ is an enhancer of adult prothoracic segment phenotype of E(z)^Trm (Bajusz et al., 2001) ash2¹ is an enhancer of eye phenotype of CycE^JP (Brumby et al., 2002) ash2¹ is an enhancer of phenotype of Antp^Ns (Adamson and Shearn, 1996)
NOT Enhancer of
Statement Reference ash2[+]/ash2¹ is a non-enhancer of haltere phenotype of Dsp1¹ (Decoville et al., 2001) ash2[+]/ash2¹ is a non-enhancer of wing \| ectopic phenotype of Dsp1¹ (Decoville et al., 2001) ash2¹ is a non-enhancer of eye phenotype of Scer\GAL4^ey.PH, osa^Scer\UAS.cCa (Collins et al., 1999) ash2¹ is a non-enhancer of wing margin phenotype of Scer\GAL4^vg.PM, sgg^S9A.Scer\UAS (Collins and Treisman, 2000) ash2¹ is a non-enhancer of wing phenotype of Scer\GAL4^vg.PM, sgg^S9A.Scer\UAS (Collins and Treisman, 2000)
Suppressor of
Statement Reference ash2¹ is a suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, dm^Scer\UAS.cZa (Secombe et al., 2007)
NOT Suppressor of
Statement Reference ash2¹ is a non-suppressor of eye phenotype of Scer\GAL4^ey.PH, osa^Scer\UAS.cCa (Collins et al., 1999) ash2¹ is a non-suppressor of wing margin phenotype of Scer\GAL4^vg.PM, sgg^S9A.Scer\UAS (Collins and Treisman, 2000) ash2¹ is a non-suppressor of wing phenotype of Scer\GAL4^OK386, nej^Scer\UAS.cMa (Bantignies et al., 2000) ash2¹ is a non-suppressor of wing phenotype of Scer\GAL4^vg.PM, sgg^S9A.Scer\UAS (Collins and Treisman, 2000)
Other
Statement Reference Df(3R)red-P93, ash2¹ has haltere phenotype (Shearn, 1989) Df(3R)red-P93, ash2¹ has metathoracic leg phenotype (Shearn, 1989)
Additional Comments
Genetic Interactions
Statement Reference sktl^Δ15/+ ; ash2¹/ash2¹⁸ larvae have delayed development and die at an earlier stage than ash2¹/ash2¹⁸ larvae. (Cheng and Shearn, 2004) ash2¹/+ does not significantly enhance the frequency of transformation of halteres into wings seen in Dsp1¹/Y flies. (Decoville et al., 2001) The addition of ash2¹ does not effect the variable eye size phenotype seen in osa^Scer\UAS.cCa/Scer\GAL4^ey.PH flies. (Collins et al., 1999) Flies heterozygous for both Df(3R)red-P93 and ash2¹ express a weak transformation of halteres to wings or third legs to second legs. (Shearn, 1989)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	ash2^I1 (Amoros et al., 2002)
Comments
Stocks ( 2 )
Bloomington	4584 red¹ e¹ ash2¹/TM6B, Tb¹
Kyoto	107807 red¹ e¹ ash2¹/TM6B, Tb¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	703 ash2¹ (Ji et al., 2012, Secombe et al., 2007, Christensen et al., 2008.4.15, Vázquez et al., 2008) ash2⁷⁰³ (Cheng and Shearn, 2004, Adamson and Shearn, 1996) ash-2⁷⁰³ (Shearn, 1989) mad¹ mad⁷⁰³
Name Synonym
Secondary FlyBase IDs

References ( 18 )
Research paper	Ji et al., 2012, G3 (Bethesda) 2(12): 1651--1660 In Vivo Regulation of E2F1 by Polycomb Group Genes in Drosophila. [FBrf0220353] Vázquez et al., 2008, Genetics 180(2): 835--843 γTub23C Interacts Genetically With Brahma Chromatin-Remodeling Complexes in Drosophila melanogaster. [FBrf0206007] Secombe et al., 2007, Genes Dev. 21(5): 537--551 The Trithorax group protein Lid is a trimethyl histone H3K4 demethylase required for dMyc-induced cell growth. [FBrf0193047] Cheng and Shearn, 2004, Genetics 167(3): 1213--1223 The direct interaction between ASH2, a Drosophila trithorax group protein, and SKTL, a nuclear phosphatidylinositol 4-phosphate 5-kinase, implies a role for phosphatidylinositol 4,5-bisphosphate in maintaining transcriptionally active chromatin. [FBrf0179167] Gutierrez et al., 2003, Development 130(2): 343--354 The Drosophila trithorax group gene tonalli(tna) interacts genetically with the Brahma remodeling complex and encodes an SP-RING finger protein. [FBrf0155711] Amoros et al., 2002, Int. J. Dev. Biol. 46(3): 321--324 The ash2 gene is involved in Drosophila wing development. [FBrf0149044] Brumby et al., 2002, EMBO J. 21(13): 3377--3389 Drosophila cyclin E interacts with components of the Brahma complex. [FBrf0151282] Bajusz et al., 2001, Genetics 159(3): 1135--1150 The Trithorax-mimic allele of Enhancer of zeste renders active domains of target genes accessible to polycomb-group-dependent silencing in Drosophila melanogaster. [FBrf0141551] Decoville et al., 2001, Genetics 157(1): 237--244 DSP1, an HMG-like protein, is involved in the regulation of homeotic genes. [FBrf0132331] Lopez et al., 2001, Mol. Genet. Genomics 266(4): 572--583 corto genetically interacts with Pc-G and trx-G genes and maintains the anterior boundary of Ultrabithorax expression in Drosophila larvae. [FBrf0145129] Bantignies et al., 2000, Mol. Cell. Biol. 20(24): 9317--9330 Functional interaction between the coactivator Drosophila CREB-binding protein and ASH1, a member of the trithorax group of chromatin modifiers. [FBrf0131248] Collins and Treisman, 2000, Genes Dev. 14(24): 3140--3152 Osa-containing Brahma chromatin remodeling complexes are required for the repression of Wingless target genes. [FBrf0132322] Collins et al., 1999, EMBO J. 18(24): 7029--7040 Osa associates with the Brahma chromatin remodeling complex and promotes the activation of some target genes. [FBrf0124978] Papoulas et al., 1998, Development 125(20): 3955--3966 The Drosophila trithorax group proteins BRM, ASH1 and ASH2 are subunits of distinct protein complexes. [FBrf0104901] Adamson and Shearn, 1996, Genetics 144(2): 621--633 Molecular genetic analysis of Drosophila ash2, a member of the trithorax group required for imaginal disc pattern formation. [FBrf0090426] LaJeunesse and Shearn, 1995, Mech. Dev. 53(1): 123--139 Trans-regulation of thoracic homeotic selector genes of the Antennapedia and bithorax complexes by the trithorax group genes: absent, small, and homeotic discs 1 and 2. [FBrf0085406] Shearn, 1989, Genetics 121: 517--525 The ash-1, ash-2 and trithorax genes of Drosophila melanogaster are functionally related. [FBrf0049909]
Personal communication to FlyBase	Christensen et al., 2008.4.15, Isolation and characterization of Df(3R)BSC492. Isolation and characterization of Df(3R)BSC492. [FBrf0204520]

Allele Dmel\ash21

Allele Dmel\ash2¹