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General Information
Symbol
Dmel\aubHN2
Species
D. melanogaster
Name
FlyBase ID
FBal0000798
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
aubHN
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C10998669T

Amino acid change:

Q622term | aub-PA; Q551term | aub-PC

Reported amino acid change:

Q622term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q622term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Embryos from aubHN2/aubQC42 mothers are completely lethal and frequently display fusion of dorsal appendages.

aubHN2/aubQC42 and aubHN2/aubsting-1 transheterozygotes exhibit crystalline aggregates (of Stellate protein) in spermatocytes, which are absent in aubHN2 single heterozygotes and wild-type controls; aubHN2/aubQC42 transheterozygotes do not display significant changes in the length of third instar larval neuromuscular junctions, as compared to controls.

aubHN2/aubQC42 mutant females exhibit a modest decrease in egg laying capacity (75% of wild type). Very few compound egg chambers (multiple cysts in a single follicular epithelium) are seen.

Germline stem cells and cystoblasts of aubN11/aubHN ovaries show delays in prometaphase and chromosome segregation defects.

Eggs laid by aubHN2/aubQC42 females have dorsal appendage defects; 52.4% have fused dorsal appendages, 24.5% lack dorsal appendages and 23.1% have wild-type dorsal appendages.

No embryos derived from aubN11/aubHN2 females produce a cuticle.

Eggs laid by aubQC42/aubHN females show a variable ventralised phenotype.

aubHN/aubQC42 eggs show fused dorsal appendages in 40% of cases, no appendages in 12% of cases and the wild-type number of appendages in 48% of cases.

Perinuclear nuage appears smoother than normal in aubHN/aubQC42 nurse cells, but nuage particles are unaffected.

2% of eggs from homozygous mothers are fertilized and embryos lack abdominal segments and pole cells, i.e. show a classic posterior group mutant phenotype.

Most extreme eggs are spindle shaped without dorsal appendages. Other eggs have fused dorsal appendages or of normal morphology but remain unfertilized.

female-sterile

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

aub[+]/aubHN2 is an enhancer of abnormal meiotic cell cycle phenotype of RanGAPSd

NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Other
Phenotype Manifest In
Suppressed by
Enhancer of
Statement
Reference

aub[+]/aubHN2 is an enhancer of abdominal segment phenotype of nos+1+3.Tag:MS2, nosBN

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

aubHN2 heterozygosity suppresses the mortality exhibited by flamA, ctA double homozygotes.

Neither aubHN2 heterozygosity nor the combination of aubHN2 heterozygosity and expression of aubScer\UAS.cBa under the control of Scer\GAL4upd1.PU are able to suppress the crystalline aggregates (of Stellate protein) observed in the spermatocytes of Fmr1Δ113M heterozygotes.

AGO1k08121, aubHN2 double homozygotes do not display significant changes in the length of third instar larval neuromuscular junctions, as compared to controls.

CycJnull/Df(3L)Exel6095 (expressing armi+t6.3 to restore armi[+] function) enhances the fertility defects seen in aubHN2/aubQC42 mutant females. The resulting flies lay no eggs and oocyte development is arrested, with egg chambers rarely advancing beyond stage 7-8. Compound egg chambers (multiple cysts in a single follicular epithelium) are seen, and these often include a disorganised layer of follicle cells almost separating two fully developed cysts. This phenotype can be fully suppressed by expression of CycJ+t4.

The segregation distortion seen in males carrying either the SD-5star or SD-LosArenos Dp(2;2)RanGAPSD chromosome variant (in a sensitive Rsp background) is enhanced (the k[[c]] value, or proportion of Dp(2;2)RanGAPSD-bearing progeny as the fraction of total progeny (corrected for viability), is significantly increased) if the males are also carrying a single copy of aubHN2.

The survival of roX1ex33A Df(1)roX2Δ adult males is not significantly altered if they are also heterozygous for aubHN2.

nosBN,nos+1+3.T:MS2\MCP.BS/nosBN embryos carrying a aubHN/+ mutation display a reduction in abdominal segmentation, forming an average of 3-4 abdominal segments.

The dorsal appendage defects seen in eggs laid by aubHN2/aubQC42 females are suppressed if the females are also homozygous for lokp6; 99.2% of the eggs have wild-type dorsal appendages.

The ventralised phenotype seen in eggs derived from aubQC42/aubHN females is partially suppressed by lokunspecified.

The fused dorsal appendage phenotype of aubHN/aubQC42 eggs is suppressed in lokp6; aubHN/aubQC42 eggs with 98% of double mutants showing wild-type appendage morphology.

The mei-41D3 mutation suppresses the fused dorsal appendage phenotype of aubHN/aubQC42 eggs; 85% of mei-41D3; aubHN/aubQC42 eggs show wild-type appendage morphology.

The yellowish eye colour caused by expression of one copy of wdsRNA.GMR in a wild-type background is not altered if the flies are also carrying aubHN2/aubN11.

Ectopic pole cells are not produced at the anterior of embryos derived from aubN11/aubHN ; oskshort.bcd females.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

The maternal effect embryonic lethality and dorsal appendages fusion phenotypes characteristic for embryos from aubHN2/aubQC42 mothers are rescued to a varying extent by expression of either aubScer\UAS.P\T.T:Avic\GFP-m6 or aubAA.Scer\UAS.P\T.T:Avic\GFP-m6 under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the embryos.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 2 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
Synonyms and Secondary IDs (5)
References (65)