Allele Dmel\aub^HN2

General Information
Symbol	Dmel\aub^HN2	Species	D. melanogaster
Name		FlyBase ID	FBal0000798
Feature type	allele	Associated gene	Dmel\aub
Also Known As	aub^HN

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Schupbach and Wieschaus, 1991)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2L:10,998,669..10,998,669 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental na_change=C10998669T pr_change=Q622\|aub-PA,Q551\|aub-PC reported_pr_change=Q622@ (Harris and Macdonald, 2001)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name AF334408 AAK43547

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: Q622@. (Harris and Macdonald, 2001)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Schupbach and Wieschaus, 1991, Chen et al., 2007) lethal \| maternal effect \| recessive (Wilson et al., 1996) mitotic cell cycle defective \| female (with aub^N11) (Pek and Kai, 2011)
Phenotype Manifest In
	chorion (with aub^QC42) (Chen et al., 2007) cystoblast (with aub^N11) (Pek and Kai, 2011) dorsal appendage (with aub^QC42) (Klattenhoff et al., 2007) embryo \| maternal effect \| posterior (Wilson et al., 1996) embryonic/larval cuticle \| embryonic stage \| maternal effect (with aub^N11) (Rouget et al., 2010) female germline stem cell (with aub^N11) (Pek and Kai, 2011)
Detailed Description
	Statement Reference Germline stem cells and cystoblasts of aub[N11]/aub[HN] ovaries show delays in prometaphase and chromosome segregation defects. (Pek and Kai, 2011) No embryos derived from aub[N11]/aub[HN2] females produce a cuticle. (Rouget et al., 2010) Eggs laid by aub[QC42]/aub[HN] females show a variable ventralised phenotype. (Chen et al., 2007) aub^HN/aub^QC42 eggs show fused dorsal appendages in 40% of cases, no appendages in 12% of cases and the wild-type number of appendages in 48% of cases. (Klattenhoff et al., 2007) Perinuclear nuage appears smoother than normal in aub^HN/aub^QC42 nurse cells, but nuage particles are unaffected. (Snee and Macdonald, 2004) 2% of eggs from homozygous mothers are fertilized and embryos lack abdominal segments and pole cells, i.e. show a classic posterior group mutant phenotype. (Wilson et al., 1996) Most extreme eggs are spindle shaped without dorsal appendages. Other eggs have fused dorsal appendages or of normal morphology but remain unfertilized. (Schupbach and Wieschaus, 1991) female-sterile
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT Suppressor of
Statement Reference aub^HN2/aub^N11 is a non-suppressor of eye color defective phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Pelisson et al., 2007)
Phenotype Manifest In
Suppressed by
Statement Reference aub^HN2/aub^QC42 has chorion phenotype, suppressible \| partially by lok^unspecified (Chen et al., 2007) aub^HN2/aub^QC42 has dorsal appendage phenotype, suppressible by lok^p6/lok^p6/lok^p6/lok^p6 (Klattenhoff et al., 2007) aub^HN2/aub^QC42 has dorsal appendage phenotype, suppressible by mei-41^D3/mei-41^D3 (Klattenhoff et al., 2007)
Enhancer of
Statement Reference aub[+]/aub^HN2 is an enhancer of abdominal segment phenotype of nos^{+1+3.T:MS2\MCP.BS}, nos^BN (Becalska et al., 2011)
Suppressor of
Statement Reference aub^HN2/aub^N11 is a suppressor of pole cell \| ectopic phenotype of osk^short.bcd (Harris and Macdonald, 2001) aub^HN2 is a suppressor of phenotype of osk^{bcd.3UTR.T:Zzzz\His6} (Wilson et al., 1996, Wilson et al., 1996)
NOT Suppressor of
Statement Reference aub^HN2/aub^N11 is a non-suppressor of eye phenotype of w^dsRNA.GMR/w^dsRNA.GMR (Pelisson et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference nos[BN],nos[+1+3.T:MS2\MCP.BS]/nos[BN] embryos carrying a aub[HN]/+ mutation display a reduction in abdominal segmentation, forming an average of 3-4 abdominal segments. (Becalska et al., 2011) The ventralised phenotype seen in eggs derived from aub[QC42]/aub[HN] females is partially suppressed by lok[unspecified]. (Chen et al., 2007) The fused dorsal appendage phenotype of aub^HN/aub^QC42 eggs is suppressed in lok^p6; aub^HN/aub^QC42 eggs with 98% of double mutants showing wild-type appendage morphology. The mei-41^D3 mutation suppresses the fused dorsal appendage phenotype of aub^HN/aub^QC42 eggs; 85% of mei-41^D3; aub^HN/aub^QC42 eggs show wild-type appendage morphology. (Klattenhoff et al., 2007) The yellowish eye colour caused by expression of one copy of w[dsRNA.GMR] in a wild-type background is not altered if the flies are also carrying aub[HN2]/aub[N11]. (Pelisson et al., 2007) Ectopic pole cells are not produced at the anterior of embryos derived from aub^N11/aub^HN ; osk^short.bcd females. (Harris and Macdonald, 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	8517 aub^HN2 cn¹ bw¹/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	aub^HN2 (Zhang et al., 2011, Anne et al., 2007, Anne and Mechler, 2005, Tomari et al., 2004, Cook et al., 2004, Findley et al., 2003, Kennerdell et al., 2002, Harris and Macdonald, 2001, Wilson et al., 1996, Wilson et al., 1996, Schupbach and Wieschaus, 1991, Klattenhoff et al., 2007, Grimaud et al., 2006, Vagin et al., 2006, Lim and Kai, 2007, Pek and Kai, 2011, Snee and Macdonald, 2004, Anand and Kai, 2012, Lim and Kai, 2007, Pelisson et al., 2007, Lim et al., 2009, Li et al., 2009, Patil and Kai, 2010, Pek et al., 2009, Creed et al., 2010, Nagao et al., 2010, Becalska et al., 2011, Pek and Kai, 2011, Rouget et al., 2010, Zamparini et al., 2011, Pöyhönen et al., 2012) aub^HN (Ashraf et al., 2006, Chen et al., 2007, Chen et al., 2007, Shpiz et al., 2009) aubHN2 (Brennecke et al., 2007)
Name Synonym
Secondary FlyBase IDs

References ( 38 )

Generate a list of	All references relating to this allele ( 38 )

List References by type	Research paper ( 34 ) Supplementary material ( 3 ) Erratum ( 1 )
Recent research papers ( 7 )
	Anand and Kai, 2012, EMBO J. 31(4): 870--882 The tudor domain protein Kumo is required to assemble the nuage and to generate germline piRNAs in Drosophila. [FBrf0217541] Pöyhönen et al., 2012, G3 (Bethesda) 2(3): 331--338 Homology-dependent silencing by an exogenous sequence in the Drosophila germline. [FBrf0217712] Becalska et al., 2011, Dev. Biol. 349(1): 46--52 Aubergine is a component of a nanos mRNA localization complex. [FBrf0212348] Pek and Kai, 2011, Proc. Natl. Acad. Sci. U.S.A. 108(29): 12007--12012 DEAD-box RNA helicase Belle/DDX3 and the RNA interference pathway promote mitotic chromosome segregation. [FBrf0214405] Pek and Kai, 2011, Curr. Biol. 21(1): 39--44 A role for vasa in regulating mitotic chromosome condensation in Drosophila. [FBrf0212718] Zamparini et al., 2011, Development 138(18): 4039--4050 Vreteno, a gonad-specific protein, is essential for germline development and primary piRNA biogenesis in Drosophila. [FBrf0214783] Zhang et al., 2011, Mol. Cell 44(4): 572--584 Heterotypic piRNA Ping-Pong Requires Qin, a Protein with Both E3 Ligase and Tudor Domains. [FBrf0216809] Show all research papers ...

Allele Dmel\aubHN2

Allele Dmel\aub^HN2