Allele Dmel\awd^K

General Information
Symbol	Dmel\awd^K	Species	D. melanogaster
Name	Killer of prune	FlyBase ID	FBal0000811
Feature type	allele	Associated gene	Dmel\awd

Map ( GBrowse )
Allele class	neomorphic allele - genetic evidence
Mutagen
Recent Updates
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FB2012_01
FB2011_10
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Nature of the Allele
Allele class	neomorphic allele - genetic evidence (Zinyk et al., 1993)
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3R:27,571,153..27,571,153 evidence=experimental pr_change=P97S reported_pr_change=P97S na_change=C27560519T (Lascu et al., 1992)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: S97P. (Timmons et al., 1995) Amino acid replacement: P?S. (Timmons and Shearn, 1995) Amino acid replacement: P97S. (Lascu et al., 1992) The levels of awd transcript are normal in (1) pn, (2) awd^K/+, and (3) pn; awd^K/+ flies; however, only the first two genotypes accumulate normal levels of the awd polypeptide, the last having little or none, despite the presence of one copy of awd⁺.
Carried on aberration	C(3R)RM-SH4b
Cytology
Phenotypic Data
Phenotypic Class
	viable (Timmons et al., 1995, Sturtevant, 1955)
Phenotype Manifest In

Detailed Description
	Statement Reference Homozygous ovaries occasionally show degenerating egg chambers at early stages (5% of ovarioles), but no significant epithelial defects are seen in the follicle cells. (Woolworth et al., 2009) Homozygous larvae develop normally. (Timmons et al., 1995) A small number of pn; awd^K larvae reach second instar. A maternal effect can shift the phenocritical period for one larval stage. This effect is temperature sensitive: low temperature, more larvae reach third instar. The perineurium, glia and lymph gland are the main cellular targets of the pn; awd^K interaction. (Hackstein, 1992) No phenotypic effects, either when homozygous or heterozygous. (Sturtevant, 1955) No phenotypic effects, either when homozygous or heterozygous; however, it is a dominant conditional lethal, which is lethal in combination with pn, i.e., pn/Y; awd^K/+ males or pn/pn; awd^K/+ females. awd^K does not interact with any other eye-color mutant. Interacts lethally with all standard alleles of pn tested; however, pn^ts-ek insensitive to awd^K at permissive temperatures, whereas nine other temperature-sensitive alleles of pn are insensitive under all conditions. Lethal phase of pn; awd^K genotypes begins in early second larval instar and lasts until after the time that normal larvae pupate. Using pn^ts-ek, the temperature-sensitive period of the pn component of the lethal interaction determined to begin in second instar and last until eclosion. pn; awd^K sons of pn mothers appear to die earlier than those of pn/+ mothers, suggesting a maternal effect of pn⁺, which is revealed in the presence of awd^K (FBrf0023024).
External Data
Linkouts
Interactions
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Phenotypic Class
NOT Enhancer of
Statement Reference awd^K/awd^K is a non-enhancer of paralytic \| heat sensitive phenotype of shi^CK2 (Krishnan et al., 2001, Krishnan et al., 2001)
Suppressor of
Statement Reference awd^K is a suppressor of lethal phenotype of hop^Tum (Zinyk et al., 1993)
Other
Statement Reference awd^K, pn¹ has lethal \| second instar larval stage phenotype (Sturtevant, 1955, Sturtevant, 1955) awd^K, pn¹ has lethal phenotype (Krishnan et al., 2001, Timmons and Shearn, 1996, Krishnan et al., 2001, Timmons and Shearn, 1996) awd^K, pn² has lethal \| second instar larval stage phenotype (Sturtevant, 1955, Sturtevant, 1955) awd^K, pn² has lethal phenotype (Timmons and Shearn, 1996, Timmons and Shearn, 1996) awd^K, pn³ has lethal \| second instar larval stage phenotype (Sturtevant, 1955, Sturtevant, 1955) awd^K, pn³ has lethal phenotype (Timmons and Shearn, 1996, Timmons and Shearn, 1996) awd^K, pn^12C has lethal phenotype (Timmons and Shearn, 1996, Timmons and Shearn, 1996) awd^K, pn^18a has lethal phenotype (Timmons and Shearn, 1996, Timmons and Shearn, 1996) awd^K, pn³⁸ has lethal phenotype (Timmons and Shearn, 1996, Timmons and Shearn, 1996) awd^K, pn^77C33 has lethal \| dominant phenotype (Timmons et al., 1995) awd^K, pn^77C33 has lethal phenotype (Timmons and Shearn, 1996, Timmons and Shearn, 1996, Timmons et al., 1995) awd^K, pn^A has lethal phenotype (Timmons and Shearn, 1996, Timmons and Shearn, 1996) awd^K, pn^pw2 has lethal phenotype (Timmons and Shearn, 1996, Timmons and Shearn, 1996) awd^K, pn^unspecified has lethal \| dominant \| larval stage phenotype awd^K, pn^unspecified has lethal \| dominant phenotype (Timmons and Shearn, 1995) awd^K/awd^KRS6, pn¹ has lethal phenotype (Krishnan et al., 2001, Krishnan et al., 2001) awd^K/awd^MSM95, pn¹ has lethal phenotype (Krishnan et al., 2001, Krishnan et al., 2001)
Phenotype Manifest In
Suppressor of
Statement Reference awd^K is a suppressor of hemocyte phenotype of hop^Tum (Zinyk et al., 1993) awd^K is a suppressor of melanotic mass phenotype of hop^Tum (Zinyk et al., 1993)
Additional Comments
Genetic Interactions
Statement Reference Lethality of awd^K/+, pn^77C33 double mutants is not due to an inadequate level of awd NDP kinase activity. (Timmons et al., 1995) Rescues lethality of hop^Tum, alleviating premature formation of circulating haemocytes and melanotic mass formation in temperature- dependent fashion. (Zinyk et al., 1993)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	awd^K is rescued by awd^+m.cPa (Provost et al., 2006)
Comments	Allelism of awd^K demonstrated by two observations. First, induced revertants of awd^K, as recovered on the basis of their failure to interact lethally with pn, are lethal in all pairwise combinations (FBrf0020566) and in combination with awd^b3. Second transformants carrying awd^K DNA are able to rescue awd^b3 lethality and are able to kill pn in the presence of homozygous awd⁺ (FBrf0051397).
Stocks ( 14 )
Bloomington	461 ca¹ awd^K 3143 Df(1)bb158, y¹/Dp(1;Y)y⁺/C(1)*; ca¹ awd^K 4242 C(1)DX, y¹ f¹/Dp(1;Y)y⁺; ca¹ awd^K 1296 C(1)M3, y¹/Dp(1;Y)y⁺; P{wA^R}tsh^4-3; awd^K 388 S¹/In(2L)Cy, In(2R)Cy, Cy¹ E(S)¹ nimA¹; awd^K 1018 C(1)M3, y¹/Dp(1;Y)y⁺; P{A92}tshⁱ⁷¹; ca¹ awd^K
Kyoto	101584 ca¹ awd^K 105923 ca¹ awd^K 106876 Df(1)bb158, y¹/Dp(1;Y)y⁺/C(1)*; ca¹ awd^K 130387 CyO/Pu²; In(3LR)HR33/T(Y;3)B116, B^S: ca¹ awd^K 106268 C(1)M3, y¹/Dp(1;Y)y⁺; P{wA^R}tsh^4-3; awd^K 105884 S¹/In(2L)Cy, In(2R)Cy, Cy¹ E(S)¹ nimA¹; awd^K
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Notes on Origin
Discoverer	Sturtevant, Jan. 1954.

Comments
	P-element insertion carrying awd^K construct rescued the lethality of awd^b3 and an insertion into the third chromosome was alone sufficient to cause an interaction with pn. (Biggs et al., 1988) Fate mapping in pn/pn/+; awd^K/+ gynandromorphs places the focus of the lethal interaction in the midventral part of the blastoderm, not a focus of pteridine biosynthesis. awd^K eye discs transplanted into pn hosts develop autonomously as do the reciprocal transplants (FBrf0011893).
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	awd^K awd^Kpn (Provost et al., 2006, Krishnan et al., 2001, Timmons and Shearn, 1995, Timmons and Shearn, 1995) awd^K-pn (Dearolf, 1998, de la Rosa et al., 1995, Watson et al., 1994, Venkatesh and Teng, 1992, Hackstein, 1992, Teng et al., 1991, Biggs et al., 1988, Woolworth et al., 2009) Kpn (Provost et al., 2006, Timmons et al., 1995, ) K-pn (Sturtevant, 1955, Lascu et al., 1992, ) unnamed (Inoue et al., 1996)
Name Synonym	Killer of prune (Provost et al., 2006, )
Secondary FlyBase IDs

References ( 22 )

Generate a list of	All references relating to this allele ( 22 )

List References by type	Research paper ( 16 ) Review ( 3 ) Abstract ( 2 ) Letter ( 1 )
Recent research papers (0)
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Allele Dmel\awdK

Allele Dmel\awd^K