Open Close
General Information
Symbol
Dmel\bas1
Species
D. melanogaster
Name
FlyBase ID
FBal0001018
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

On mechanical agitation, bas1 flies show a stereotypical behavioral sequence of initial spasm, paralysis, delayed spasm and recovery of normal posture. The two spasms are manifested by collapse of the body, high-frequency wing flapping, leg extension, and fully curved abdomen. Seizing females often lay eggs. Electroconvulsive stimulation delivered to the brain of bas1 flies reproducibly induces the bang-sensitive repertoire of seizure behaviour; such electroconvulsive seizures can actually be induced in wild-type flies but this requires more extreme stimulus intensities than for bas1 flies. The failure and recovery of the giant fibre (GF) pathway during seizure can be detected by recording the dorsal longitudinal muscle (DLM) physiological response. Following 1 Hz brain stimulation, bas1 mutants have a significantly longer period of DLM response failure compared to wild type. Additionally, the maximal initial discharge induction is shifted toward lower stimulus intensities in the mutant flies. Delivery of a second stimulus after the onset of the delayed discharge (DD) phase of the DLM response causes a brief suppression of DD and a slight delay in response recovery, an effect that is more pronounced in bas1 mutants than wild type. Delivery of a second stimulus after seizure recovery induces a refractory period during which DLM response failure is shortened and the DD stage is earlier and for a shorter duration; this refractory period is shorter in bas1 flies than in wild type.

Bang-sensitive mutant. Flies usually show abnormal spontaneous activity ("seizures") in the dorsal longitudinal muscle (DLM) lasting approximately 0.5-3 seconds after the delivery of an electrical buzz (50-400 msec) to the brain. Stimulation of the giant fibre (GF) usually fails to evoke DLM potentials following the buzz. This failure lasts for 46 +/- 20 seconds. There is a close correlation between the seizure and failure phenotypes; if a seizure occurs, a failure also occurs in greater than 95% of cases, while failures without seizures occurred in approximately 10% of cases. GF evoked responses by the DLM are abnormal during recovery from the buzz. After recovery, there is a refactory period during which a buzz is less effective at inducing seizures and failures.

Physiological defect: a striking reduction of spike frequency in the anterior postalar (APA) and anterior notopleural (ANP) after mechanical stimulus.

Slow recovery from the prolonged depolarization after potential (PDA). Affect retinula cell physiology.

Bang sensitive paralytic mutant. At 38oC bas mutants become paralysed, without banging, within 12-20 seconds.

Striking culture vial sharply on hard surface immobilizes bas flies for 30-40 seconds. Rapid recovery followed by refractory period of an hour. Suppressed by mlenap-ts1 at permissive temperatures; at temperatures above 37.5oC bas flies quickly paralyzed; no detectable physiological abnormalities at neuromuscular junction in bas larvae (Ganetzky and Wu). In experiments on photoreceptor function, recovery of prolonged depolarization afterpotentials (induced by strong blue light) abnormally slow following exposure to orange light (Homyk and Pye, 1989).

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

bas1 has paralytic phenotype, enhanceable by parabss1

bas1 has bang sensitive phenotype, enhanceable by parabss1

NOT Enhanced by
Statement
Reference

bas1 has behavior defective phenotype, non-enhanceable by Df(2L)TW1/+

NOT suppressed by
Statement
Reference

bas1 has behavior defective phenotype, non-suppressible by Df(2L)TW1/+

Enhancer of
Statement
Reference

bas1 is an enhancer of paralytic phenotype of parabss1

bas1 is an enhancer of bang sensitive phenotype of parabss1

Phenotype Manifest In
Enhanced by
Statement
Reference
Enhancer of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Df(2L)TW1/+ does not significantly alter the seizure phenotype of bas1 hemizygotes.

Following mechanical agitation, bss1 bas1 double mutant flies have a prolonged paralysis and take longer to recover following spasm than single mutants. These double mutants also have an enhanced susceptibility to electroconvulsion compared to single mutants with a prolonged DLM response failure. The sensitivity for response failure induction and maximal initial discharge induction is similar in the double mutants to bss1 single mutants. Delivery of a second stimulus after the onset of DD causes a greater suppression of DD and a more delayed response recovery in double mutants than either single mutant. Additionally, delivery of a second stimulus after seizure recovery induces a shorter refractory period in bss1 bas1 double mutants. The jump muscle TTMs have an earlier response recovery following electroconvulsive stimuli, compared to DLMs; this effect is much more pronounced in bss1 bas1 mutants than in wild-type flies. Gynandromorph analysis shows that the recovery time of DLM responses to test stimuli applied to the brain is progressively lengthened as the number of bss1 bas1 mutant neurons in the GF pathway increases.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

No apparent defect in the larval neuromuscular junction preparation. Behavioral phenotype suppressed by mlenap-ts1, even at permissive temperatures.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (6)