A Database of Drosophila Genes & Genomes

FB2008_06, released July 3, 2008
 

Allele Dmel\Bc1

General Information
SymbolDmel\Bc1SpeciesD. melanogaster
NameFlyBase IDFBal0001073
Feature typealleleCreated / Updated2006-08-22/2006-08-22
Associated geneDmel\Bc
Allele class
Mutagenethyl methanesulfonate
hide Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Grell, 1969, Lemaitre et al., 1995)
Mapped Features and Mutations
Type
Symbol & Location
Additional Notes
References
 
 
 
 
Associated Sequence Data
DDBJ /
EMBL /
GenBank
DNA sequence
Protein sequence
Name
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Progenitor genotype
      Nature of the lesion
      Statement
      Reference
       
       
      Assay mode
      Cytology
      hide Phenotypic Data
      hide Phenotypic Class
      viable
      (Lemaitre et al., 1995, )
      lethal | recessive
      (Grell, 1969)
      visible | dominant
      (Grell, 1969)
      visible | semidominant
      (Lemaitre et al., 1995, )
      visible | conditional ts
      (Lemaitre et al., 1995)
      fertile
       
      tumorigenic | dominant
      (Lemaitre et al., 1995, Lemaitre et al., 1995)
      hide Phenotype Manifest In
      crystal cell
      (Lebestky et al., 2000, Sorrentino et al., 2002, Lebestky et al., 2000)
      hemocyte
      (Rizki and Rizki, 1981, Lemaitre et al., 1995)
      hemolymph
      (Rizki and Rizki, 1981)
      lymph gland
      (Rizki and Rizki, 1981)
      hide Detailed Description
      Statement
      Reference
      Quadrate crystalline bodies in crystal cells of hemolymph replaced by amorphous melanotic mass. Black cells appear in 11-hour embryos in Bc1/Bc1 and in late first-instar larvae in Bc1/+. Crystal cells replaced by black cells during first instar in Bc1/+. Numerousblack cells visible through the integument of larvae, pupae and of head, thorax and abdomen in adults. Bc1/Bc1 larvae have no phenol oxidase activity and larval hemolymph fails to darken upon exposure to air; Bc1/+ intermediate between +/+ and Bc1/Bc1 in these respects.
       
      Bc1 causes paracrystalline inclusions in hemocytes of larvae. Heat-induction blackens the crystal cells in the Bc1 hemolymph and lymph glands.
      (Rizki and Rizki, 1981)
      No haemolymph prophenoloxidase. Eggs of the parasitoid strain L.boulardi are encapsulated by host haemocytes but the cellular capsules do not melanize and harden.
      (Rizki and Rizki, 1990)
      Heterozygotes have numerous pigmented cells under the cuticle of the head, thorax and abdomen. This phenotype is very striking in larvae and pupae. In larvae, most of the black cells appear to be attached to the larval integument, but some are floating freely in the body cavity.
      (Grell, 1969)
      Homozygous and heterozygous larvae have circulating melanised crystals. At 21oC larvae have a high frequency of melanotic tumours and a higher them normal haemocyte count.
      (Lemaitre et al., 1995)
      Crystal cells show premature melanization of crystalline inclusions.
      (Lebestky et al., 2000)
      Crystal cells show premature melanization of crystalline inclusions.
      (Lebestky et al., 2000)
      Blackened crystal cells are not visible in Bc1/+ larvae raised at 29oC though are visible at 27oC.
      (Sorrentino et al., 2002)
      The Bc1 mutant causes spontaneous melanisation of the crystal cells.
      (Bataille et al., 2005)
      Spontaneous melanisation of the hemolymph (assayed by dissecting larvae and exposing them to air) is much lower in Bc[1] larvae than in wild type.
      (Castillejo-Lopez and Haecker, 2005)
      hide Interactions
      hide Phenotypic Class
      hide Phenotype Manifest In
      hideSuppressed by
      Statement
      Reference
      Bc1 has hemocyte phenotype, suppressible by lz36f17
      (Rizki and Rizki, 1981)
      Bc1 has hemolymph phenotype, suppressible by lz36f17
      (Rizki and Rizki, 1981)
      Bc1 has lymph gland phenotype, suppressible by lz36f17
      (Rizki and Rizki, 1981)
      Bc1 has hemocyte phenotype, suppressible by lzBS+46
      (Rizki and Rizki, 1981)
      Bc1 has hemolymph phenotype, suppressible by lzBS+46
      (Rizki and Rizki, 1981)
      Bc1 has lymph gland phenotype, suppressible by lzBS+46
      (Rizki and Rizki, 1981)
      Bc1 has hemocyte phenotype, suppressible by lzD
      (Rizki and Rizki, 1981)
      Bc1 has hemolymph phenotype, suppressible by lzD
      (Rizki and Rizki, 1981)
      Bc1 has lymph gland phenotype, suppressible by lzD
      (Rizki and Rizki, 1981)
      Bc1 has hemocyte phenotype, suppressible by lzrfg
      (Rizki and Rizki, 1981)
      Bc1 has hemolymph phenotype, suppressible by lzrfg
      (Rizki and Rizki, 1981)
      Bc1 has lymph gland phenotype, suppressible by lzrfg
      (Rizki and Rizki, 1981)
      Bc1 has hemocyte phenotype, suppressible by lzs
      (Rizki and Rizki, 1981)
      Bc1 has hemolymph phenotype, suppressible by lzs
      (Rizki and Rizki, 1981)
      Bc1 has lymph gland phenotype, suppressible by lzs
      (Rizki and Rizki, 1981)
      hide Additional Comments
      hide Genetic Interactions
      Statement
      Reference
      Five out of 15 alleles of lz suppress the Bc1 phenotype.
      (Rizki and Rizki, 1981)
      hide Xenogenetic Interactions
      Statement
      Reference
      hide Complementation & Rescue Data
      Comments
      hide Stocks ( 52 )
      Bloomington
      1362
      Bc1/CyO
      1036
      Bc1 fj1 wt1
      4456
      F(2L)1/F(2R)1, cn1 Bc1
      4131
      l(2)24Dc1/In(2LR)Gla, wgGla-1 Bc1
      4132
      l(2)24Dd1/In(2LR)Gla, wgGla-1 Bc1
      4133
      l(2)24De1/In(2LR)Gla, wgGla-1 Bc1
      2078
      y1 w*; CyO, H{PDelta2-3}HoP2.1/Bc1
      Kyoto
      106286
      Bc1/CyO
      106179
      Bc1 fj1 wt1
      101534
      l(2)24De2 / Bc1 Gla1
      101535
      l(2)24De3 / Bc1 Gla1
      101536
      l(2)24De4 / Bc1 Gla1
      hide Notes on Origin
      Discoverer
      hide Comments
      Was originally recovered with a closely linked lethal mutation.
       
      A closely linked mutation, imd1 is responsible for the severely impaired expression of the antibacterial peptide genes when larvae are bacterially challenged. The mutations are separable by recombination.
      (Lemaitre et al., 1995)
      hide Synonyms & Secondary IDs ( 5 )
      Reported As
      Symbol Synonym
      1046Bc
      (Lemaitre et al., 1995)
      Bc
      (Sorrentino et al., 2002, Lebestky et al., 2000, Lebestky et al., 2000, Braun et al., 1997, Corbo and Levine, 1996, Grell, 1969, Castillejo-Lopez and Haecker, 2005)
      Bc1
      (Kume et al., 2005)
      unnamed
      (Rizki and Rizki, 1981)
      Name Synonym
      Black cells mutant
      (Kambris et al., 2006)
      Secondary FlyBase IDs
        hide References ( 17 )
        Research paper
        Bataille et al., 2005, Development 132(20): 4635--4644
        Resolving embryonic blood cell fate choice in Drosophila: interplay of GCM and RUNX factors. [FBrf0187450]
        Castillejo-Lopez and Haecker, 2005, Biochem. biophys. Res. Commun. 338(2): 1075--1082
        The serine protease Sp7 is expressed in blood cells and regulates the melanization reaction in Drosophila. [FBrf0189827]
        Kume et al., 2005, J. Neurosci. 25(32): 7377--7384
        Dopamine is a regulator of arousal in the fruit fly. [FBrf0187901]
        Galko and Krasnow, 2004, PLoS Biol. 2(8): 1114--1126
        Cellular and genetic analysis of wound healing in Drosophila Larvae. [FBrf0179215]
        Takehana et al., 2004, EMBO J. 23(23): 4690--4700
        Peptidoglycan recognition protein (PGRP)-LE and PGRP-LC act synergistically in Drosophila immunity. [FBrf0180202]
        Sorrentino et al., 2002, Dev. Biol. 243(1): 65--80
        Cellular immune response to parasite infection in the Drosophila lymph gland is developmentally regulated. [FBrf0144807]
        Fossett et al., 2001, Proc. Natl. Acad. Sci. USA 98(13): 7342--7347
        The Friend of GATA proteins U-shaped, FOG-1, and FOG-2 function as negative regulators of blood, heart, and eye development in Drosophila. [FBrf0137102]
        Lebestky et al., 2000, Science 288(5463): 146--149
        Specification of Drosophila hematopoietic lineage by conserved transcription factors. [FBrf0127186]
        Braun et al., 1997, Genetics 147(2): 623--634
        Drosophila immunity: analysis of larval hemocytes by P-element-mediated enhancer trap. [FBrf0098749]
        Corbo and Levine, 1996, Mech. Dev. 55(2): 211--220
        Characterization of an immunodeficiency mutant in Drosophila. [FBrf0086971]
        Lemaitre et al., 1995, Proc. Natl. Acad. Sci. USA 92(21): 9465--9469
        A recessive mutation, immune deficiency (imd), defines two distinct control pathways in the Drosophila host defense. [FBrf0084125]
        Lemaitre et al., 1995, EMBO J. 14(3): 536--545
        Functional analysis and regulation of nuclear import of dorsal during the immune response in Drosophila. [FBrf0080205]
        Rizki and Rizki, 1990, J. Insect Physiol. 36(7): 523--529
        Encapsulation of parasitoid eggs in phenol oxidase-deficient mutants of Drosophila melanogaster. [FBrf0052363]
        Grell, 1969, D. I. S. 44: 46--47
        [New mutants report.] [FBrf0063488]
        Supplementary material
        Kambris et al., 2006, Curr. Biol. 16(8):
        Document S1. Two Figures and Supplemental Experimental Procedures. [FBrf0199186]
        Lebestky et al., 2000, Science 288(5463):
        Specification of Drosophila hematopoietic lineage by conserved transcription factors. [FBrf0129252]
        Abstract
        Rizki and Rizki, 1981, Genetics 97(Suppl. 1): s90
        Alleles of lz as suppressors of the Bc-phene in Drosophila melanogaster. [FBrf0036624]