FlyBase Allele Report: Dmel\bel[6]

General Information

Symbol

Dmel\bel⁶

Species

D. melanogaster

Name

FlyBase ID

FBal0001099

Feature type

allele

Associated gene

Dmel\bel

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Amino acid replacement: Q288term.

(Ihry et al., 2012)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:8,657,400..8,657,400 [-]

Nucleotide change:

C8657400T

Amino acid change:

Q288term | bel-PA; Q288term | bel-PB

Reported amino acid change:

Q288term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Ihry et al., 2012)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of Sertoli cell-only syndrome

CEA with bel^neo30

(Kotov et al., 2016)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates fragile X-associated tremor/ataxia syndrome

modeled by Zzzz\CGG^90.UAS.EGFP

(Linsalata et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cyst progenitor cell | adult stage | increased number (with bel^neo30)

(Kotov et al., 2016)

dorsal appendage (with bel^neo30)

(Johnstone et al., 2005)

egg chorion (with bel^neo30)

(Johnstone et al., 2005)

germinal proliferation center hub | adult stage (with bel^neo30)

(Kotov et al., 2016)

male germline cell | adult stage

(Kibanov et al., 2011)

male germline cell | adult stage (with bel^neo30)

(Kotov et al., 2016)

male germline stem cell | adult stage (with bel^neo30)

(Kotov et al., 2016)

male germline stem cell | third instar larval stage (with bel^neo30)

(Kotov et al., 2016)

spermatocyte | adult stage (with bel^neo30)

(Kotov et al., 2016)

spermatocyte cell spectrosome | adult stage (with bel^neo30)

(Kotov et al., 2016)

spermatocyte fusome | adult stage (with bel^neo30)

(Kotov et al., 2016)

spermatogonium | adult stage (with bel^neo30)

(Kotov et al., 2016)

spermatogonium | third instar larval stage (with bel^neo30)

(Kotov et al., 2016)

testis | adult stage

(Kibanov et al., 2011)

testis | third instar larval stage (with bel^neo30)

(Kotov et al., 2016)

Detailed Description

Statement

Reference

bel^neo30/bel⁶ mutant testes display a germ cell loss phenotype, with complete absence of vas-stained germline stem cells (GSCs) attached to the hub at the apical testis tip (but sometimes testes contain residual vas-stained germ cells that exhibit morphological defects or size abnormalities such as giant nuclei), compared to controls (bel/+ heterozygotes); half of mutant testes do not contain germ cells at all. bel^neo30/bel⁶ mutant testes do not contain wild type spectrosomes and fusomes, showing complete loss or disrupted fusome structure. Mutant bel^neo30/bel⁶ testes show severe depletion of germinal content (including GSCs, spermatogonial cells and spermatocytes). bel^neo30/bel⁶ mutants show expanded expression of hub markers and significantly more somatic cyst stem cells in the testes than controls. Older (6-7 days old) mutant male testes show an overpopulation of somatic cells and an absence of germ cells, compared to controls. bel^neo30/bel⁶ germline cells (but not somatic cyst cells) in the testis show increased rates of apoptosis compared to controls.

Overall distribution of germinal and somatic cells and hub structure in third instar larval bel^neo30/bel⁶ mutant testes is similar to controls, however mutants show significant testis size atrophy and a reduced number of GSCs; mutant spermatogonia often have abnormally large cellular and nuclear volumes, possibly due to delay in G2 growth phase.

(Kotov et al., 2016)

Adult bel⁶/bel^neo30 transheterozygotes have severely reduced testes compared to wild-type and have complete loss of germ cells prior to the spermatocyte growth stage.

(Kibanov et al., 2011)

Approximately 80% of cells in anaphase in homozygous clones in the wing disc contain lagging chromosomes.

(Pek and Kai, 2011)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

bel⁶ is a suppressor of abnormal eye color phenotype of w^RNAi.GMR

(Zhou et al., 2008)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Flies carrying w^IR.GMR in which the eyes are homozygous for bel⁶ have small, rough eyes. The eyes contain patches of increased pigmentation compared to w^IR.GMR single mutant flies.

(Zhou et al., 2008)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

bel^M2

(Matthews, 2001.4.12)

bel^M3

(Matthews, 2001.4.12)

bel^M4

(Matthews, 2001.4.12)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4024

kni^ri-1 rn^roe-1 bel⁶ p^p/TM3, Sb¹

Kyoto

107560

kni^ri-1 rn^roe-1 bel⁶ p^p / TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

Bel⁶

(Linsalata et al., 2019)

bel⁶

(Tsai et al., 2024, Olesnicky et al., 2018, Kotov et al., 2016, Lo et al., 2016, Ihry et al., 2012, Kibanov et al., 2011, Poulton et al., 2011, Zhou et al., 2008)

belle⁶

(Pek and Kai, 2011)

belle^prl34

l(3)85Ac⁷

l(3)85Ac^prl34

(Jones and Rawls, 1988)

l(3)bel^prl34

(Johnstone et al., 2005)

Name Synonyms

Secondary FlyBase IDs

FBal0010200

References (13)

Report Sections

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