Allele Dmel\bs²

General Information
Symbol	Dmel\bs²	Species	D. melanogaster
Name		FlyBase ID	FBal0001305
Feature type	allele	Associated gene	Dmel\bs

Allele class
Mutagen
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference
Cytology
Phenotypic Data
Phenotypic Class
	viable (with bs⁰³²⁶⁷) (Roch et al., 1998) visible (Rendina et al., 2010) visible (with bs⁰³²⁶⁷) (Roch et al., 1998) visible \| recessive (Baonza and Garcia-Bellido, 1999, )
Phenotype Manifest In
	wing wing (with bs⁰³²⁶⁷) (Roch et al., 1998) wing vein (Sturtevant and Bier, 1995) wing vein \| ectopic (Baonza and Garcia-Bellido, 1999, Marenda et al., 2004, Terriente-Félix and de Celis, 2009, Rendina et al., 2010) wing vein \| ectopic (with bs⁰³²⁶⁷) (Roch et al., 1998)
Detailed Description
	Statement Reference Homozygous flies have ectopic wing vein tissue. (Rendina et al., 2010) bs[2] and bs[2]/In(2LR)Px[4] mutants do not increase sleep after social enrichment. Circadian rhythms are intact in bs[2] mutants. (Donlea et al., 2009) Homozygous bs[2] mutant wings show ectopic wing vein tissue in the distal regions of the L3, L4 and most L5 veins. (Terriente-Félix and de Celis, 2009) Wings from bs[2] heterozygotes are normal. (Lyulcheva et al., 2008) Homozygotes have extra wing veins. (Baonza and Garcia-Bellido, 1999) bs⁰³²⁶⁷/bs² is a viable combination that displays almost entirely wing vein tissue in the wing. (Roch et al., 1998) Extra vein mutation. (Sturtevant and Bier, 1995) More extreme allele than bs¹. RK2.
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference bs² has visible \| recessive phenotype, enhanceable by emc¹/emc¹¹ (Baonza and Garcia-Bellido, 1999) bs² has visible phenotype, enhanceable by Bap170^hfl1/Bap170[+] (Rendina et al., 2010)
Enhancer of
Statement Reference bs[+]/bs² is an enhancer of visible phenotype of if³ (Wessendorf et al., 1992)
Suppressor of
Statement Reference bs[+]/bs² is a suppressor of visible phenotype of LIMK1^Scer\UAS.cCa, Scer\GAL4^en-e16E (Chen et al., 2004) bs² is a suppressor \| partially of visible phenotype of csw^lf (Firth et al., 2000) bs⁰³²⁶⁷/bs² is a suppressor of visible phenotype of rho⁹/rho^ve-1, vn^M2/vn¹ (Roch et al., 1998)
Phenotype Manifest In
Enhanced by
Statement Reference bs² has wing vein \| ectopic phenotype, enhanceable by Bap170^hfl1/Bap170[+] (Rendina et al., 2010) bs² has wing vein \| ectopic phenotype, enhanceable by emc¹/emc¹¹ (Baonza and Garcia-Bellido, 1999) bs² has wing vein \| ectopic phenotype, enhanceable by osa^C819/Scer\GAL4^MD-638 (Terriente-Félix and de Celis, 2009)
Suppressed by
Statement Reference bs² has wing vein \| ectopic phenotype, suppressible \| partially by brm² (Marenda et al., 2004) bs² has wing vein \| ectopic phenotype, suppressible by osa³⁰⁸/brm² (Terriente-Félix and de Celis, 2009)
NOT suppressed by
Statement Reference bs² has phenotype, non-suppressible by su(Hw)² (Lindsley and Grell, 1968)
Enhancer of
Statement Reference bs[+]/bs² is an enhancer of wing phenotype of if³ (Wessendorf et al., 1992) bs[+]/bs² is an enhancer of wing vein \| ectopic phenotype of Snr1^E1 (Marenda et al., 2004) bs² is an enhancer of wing vein \| ectopic phenotype of rho^hs.PSt (Sturtevant and Bier, 1995)
Suppressor of
Statement Reference bs[+]/bs² is a suppressor of wing phenotype of LIMK1^Scer\UAS.cCa, Scer\GAL4^en-e16E (Chen et al., 2004) bs[+]/bs² is a suppressor of wing phenotype of Scer\GAL4^Bx-MS1096, pico^{Scer\UAS.T:Zzzz\His6,T:Hsap\MYC} (Lyulcheva et al., 2008) bs² is a suppressor \| partially of wing vein phenotype of csw^lf (Firth et al., 2000) bs² is a suppressor of wing vein phenotype of rho^ve-1 (Sturtevant and Bier, 1995) bs⁰³²⁶⁷/bs² is a suppressor of wing vein phenotype of rho⁹/rho^ve-1, vn^M2/vn¹ (Roch et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference Bap170[hfl1]/+ enhances the ectopic wing vein phenotype of bs[2] homozygotes. (Rendina et al., 2010) The ectopic wing vein phenotype observed in homozygous bs[2] mutant wings is suppressed in animals doubly heterozygous for osa[308] and brm[2]. Overexpression of osa[C819] by Scer\GAL4[MD-638] enhances the ectopic veins observed in bs[2] mutant animals. (Terriente-Félix and de Celis, 2009) bs²/+ suppresses the mutant wing phenotype caused by expression of LIMK1^Scer\UAS.cCa under the control of Scer\GAL4^en-e16E (the % of wings with normal morphology at 18^oC is increased from 9% to 81%). (Chen et al., 2004) The bs² extra wing vein phenotype is enhanced by emc¹/emc¹¹. emc¹ clones in bs² wings differentiate as vein everywhere in the wing blade covering intervein regions as vein. (Baonza and Garcia-Bellido, 1999) bs⁰³²⁶⁷/bs²; rho⁹, vn^M2/rho^ve-1, vn¹ triple mutants show an almost wild type wing vein pattern. (Roch et al., 1998) Enhancement of rho^hs.PSt ectopic vein phenotype and suppression of the rho^ve-1 loss of vein phenotype. (Sturtevant and Bier, 1995) Enhances the wing blister phenotype of if³ flies. (Wessendorf et al., 1992)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	403 sp² bs²
Kyoto	105894 sp² bs²
Notes on Origin
Discoverer	Bridges, 24 Nov. 1925.

Comments
	Wing phenotype caused by same mechanism as that of px. (Waddington, 1940) bs²/Df(2R)Px1 easily separable from +/Df(2R)Px1, especially at or below 19^oC.
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	bs² (Janody and Treisman, 2006, Lyulcheva et al., 2008, Donlea et al., 2009, Terriente-Félix and de Celis, 2009, Rendina et al., 2010)
Name Synonym
Secondary FlyBase IDs

References ( 14 )
Research paper	Rendina et al., 2010, Genetics 186(1): 167--181 Bap170, a Subunit of the Drosophila PBAP Chromatin Remodeling Complex, Negatively Regulates the EGFR Signaling. [FBrf0211845] Donlea et al., 2009, Science 324(5923): 105--108 Use-dependent plasticity in clock neurons regulates sleep need in Drosophila. [FBrf0207700] Terriente-Félix and de Celis, 2009, Dev. Biol. 329(2): 350--361 Osa, a subunit of the BAP chromatin-remodelling complex, participates in the regulation of gene expression in response to EGFR signalling in the Drosophila wing. [FBrf0208056] Lyulcheva et al., 2008, Dev. Cell 15(5): 680--690 Drosophila pico and its mammalian ortholog lamellipodin activate serum response factor and promote cell proliferation. [FBrf0206242] Janody and Treisman, 2006, Development 133(17): 3349--3357 Actin capping protein alpha maintains vestigial-expressing cells within the Drosophila wing disc epithelium. [FBrf0193788] Chen et al., 2004, Curr. Biol. 14(4): 309--313 Rho-LIM kinase signaling regulates ecdysone-induced gene expression and morphogenesis during Drosophila metamorphosis. [FBrf0174477] Marenda et al., 2004, Dev. Biol. 267(2): 279--293 The Drosophila Brahma (SWI/SNF) chromatin remodeling complex exhibits cell-type specific activation and repression functions. [FBrf0174517] Firth et al., 2000, Genetics 156(2): 733--748 Identification of genomic regions that interact with a viable allele of the Drosophila protein tyrosine phosphatase corkscrew. [FBrf0129810] Baonza and Garcia-Bellido, 1999, Mech. Dev. 80(2): 133--146 Dual role of extramacrochaetae in cell proliferation and cell differentiation during wing morphogenesis in Drosophila. [FBrf0107601] Roch et al., 1998, Development 125(10): 1823--1832 Genetic interactions and cell behaviour in blistered mutants during proliferation and differentiation of the Drosophila wing. [FBrf0102828] Sturtevant and Bier, 1995, Development 121(3): 785--801 Analysis of the genetic hierarchy guiding wing vein development in Drosophila. [FBrf0076146] Wessendorf et al., 1992, Cold Spring Harbor Symp. Quant. Biol. 57: 241--248 Genetic interactions with integrins during wing morphogenesis in Drosophila. [FBrf0064744] Waddington, 1940, J. Genet. 41: 75--139 The genetic control of wing development in Drosophila. [FBrf0005070]
Book	Lindsley and Grell, 1968, Publs Carnegie Instn 627: 469pp Genetic variations of Drosophila melanogaster. [FBrf0020044]

Allele Dmel\bs2

Allele Dmel\bs²