General Information
Symbol
Dmel\bsk1
Species
D. melanogaster
Name
FlyBase ID
FBal0001321
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
bsk1
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
G10248232A
Reported nucleotide change:
G?A
Amino acid change:
G225E | bsk-PB; G225E | bsk-PE; G225E | bsk-PF
Reported amino acid change:
G225E
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: G225E.
Mutation is located between subdomains IX and X of the bsk kinase, is conserved in the MAP kinase family and is located in a region required for substrate recognition by JNK protein kinases.
Nucleotide substitution: G?A.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Model Data
Disease Ontology
Models ( 1 )
Disease
Evidence
References
inferred from mutant phenotype
Interactions ( 1 )
Disease
Interaction
References
Comments ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
bsk1 heterozygosity does not significantly affect the mitotic index in the adult midgut epithelium, as compared to controls.
bsk1/bsk1 clones (in adult mushroom body γ lobe) are deficient in axon (but not dendrite) pruning compared to wild type.
bsk1/+ third instar larvae do not show any decrease in the neuromuscular junction bouton number.
bsk1 heterozygous mutants exhibit extreme sensitivity to 1-octen-3-ol. These flies survive for approximately 15 days when exposed to 0.5ppm 1-octen-3-ol, significantly lower than the 18 days-long survival span observed for exposed wild-type flies.
As in wild type, a bsk1 mutant neuroblast clone in the larval brain maintains a single neuroblast and consists of many neuronal progeny.
Single cell bsk1 clones in mushroom bodies have axons with wild type projections.
bsk1/bsk2 mutant embryos exhibit defective dorsal closure. F-actin cable formation in the peripheral amnioserosa is less prominent and the outer (ventral) edge of the peripheral amnioserosa is able to maintain a higher activity of filopodia.
Approximately 60% of bsk1 homozygous embryos shows a 'dorsal open' phenotype.
Mutant embryos have dorsal holes in the cuticle.
Homozygous embryos show a weak dorsal-open phenotype.
Homozygotes show a defect in dorsal closure.
Homozygous embryos show defects in dorsal closure.
Embryos exhibit a dorsal open phenotype.
Embryonic cuticle is partially closed in the dorsal posterior part. Removal of maternal and zygotic bsk (bsk1/Df(2L)flp147E) results in a complete dorsal open phenotype.
Initial phases of dorsal closure are normal, but the first rows under the leading cell row of the dorsal edge show only a partial lengthening cell shape change or no cell shape change at all. The defect is more pronounced in anterior cells. Embryonic cuticle defect is temperature sensitive: At 18oC egg lays show weaker phenotypes. Embryos derived from germline clones show an extreme dorsal open phenotype, with no cell shape change in leading row cells, and dorsal closure never initiates. This phenotype is rescuable by zygotic bsk+. Homozygous clones in the eye reveal no abnormality.
Mutant embryos show a dorsal hole due to a defect in dorsal closure. Dorsoventral patterning is not defective.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
NOT suppressed by
Statement
Reference
bsk1 has lethal phenotype, non-suppressible by acal[+]/lncRNA:acal5
Enhancer of
Statement
Reference
bsk1/bsk[+] is an enhancer of visible phenotype of Pknk06808
bsk1/bsk[+] is an enhancer of partially lethal - majority die phenotype of slprBS06
bsk1/bsk[+] is an enhancer of visible phenotype of by2
NOT Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
bsk1 is a suppressor | partially of lethal phenotype of lncRNA:acal5
bsk1/bsk[+] is a suppressor of neurophysiology defective phenotype of ninaE17, ninaEP37H
bsk1/bsk[+] is a suppressor of neuroanatomy defective phenotype of ninaE17, ninaEP37H
bsk1/bsk[+] is a suppressor | partially of visible phenotype of DrefUAS.cSa, Scer\GAL4GMR.PS
bsk1/bsk[+] is a suppressor | partially of visible phenotype of Rab11mo
bsk1/bsk[+] is a suppressor of visible phenotype of flw1, pucA251.1F3/puc[+]
bsk1/bsk[+] is a suppressor | partially of lethal | recessive phenotype of flw6
bsk1/bsk[+] is a suppressor | partially of visible phenotype of Scer\GAL4hs.2sev, Tak1UAS.cMa
bsk1/bsk[+] is a suppressor | partially of visible phenotype of Rala35d
bsk1 is a suppressor of visible phenotype of RetMEN2B.GMR
bsk1 is a suppressor of visible phenotype of RetMEN2A.GMR
bsk1 is a suppressor of lethal | pupal stage phenotype of Cskj1D8/CskS030003
bsk1/bsk[+] is a suppressor of visible phenotype of Scer\GAL469B, nmoc5-1.UAS
bsk1/bsk[+] is a suppressor | partially of planar polarity defective phenotype of peb1
bsk1/bsk[+] is a suppressor of visible phenotype of Rac1GMR.PN
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
Statement
Reference
bsk1/bsk[+] is an enhancer of wing phenotype of Pknk06808
bsk1/bsk[+] is an enhancer of wing margin bristle phenotype of Pknk06808
bsk1/bsk[+] is an enhancer of wing phenotype of by2
NOT Enhancer of
Statement
Reference
bsk1/bsk[+] is a non-enhancer of eye | somatic clone phenotype of gfr1
bsk1/bsk[+] is a non-enhancer of photoreceptor cell & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549
bsk1 is a non-enhancer of wing phenotype of Scer\GAL4dpp.PH, eyaUAS.cHa
bsk1 is a non-enhancer of phenotype of Rho1rev220
Suppressor of
Statement
Reference
bsk1/bsk[+] is a suppressor | partially of eye phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, hepCA.UAS
bsk1/bsk[+] is a suppressor of wing disc phenotype of CskGD9345, Scer\GAL4ptc-559.1
bsk1 is a suppressor of wing phenotype of DaxxEY09290, Scer\GAL4Bx-MS1096
bsk1/bsk[+] is a suppressor of eye phenotype of Mmus\Gria1Lc.UAS, Scer\GAL4hs.2sev
bsk1/bsk[+] is a suppressor of eye phenotype of Hsap\APPAβ42.GMR.Tag:SS(rPENK)
bsk1/bsk[+] is a suppressor of germline cell | embryonic stage 15 phenotype of raw155.27/raw134.47
bsk1/bsk[+] is a suppressor | partially of wing phenotype of HIV-1\VpuUAS.cLa, Scer\GAL4dpp.blk1
bsk1/bsk[+] is a suppressor | partially of eye phenotype of DrefUAS.cSa, Scer\GAL4GMR.PS
bsk1/bsk[+] is a suppressor of eye phenotype of Scer\GAL4GMR.PS, TgA.UAS
bsk1/bsk[+] is a suppressor of ommatidium phenotype of Scer\GAL4GMR.PS, TgA.UAS
bsk1/bsk[+] is a suppressor of eye phenotype of hepCA.sev.Tag:MYC
bsk1/bsk[+] is a suppressor of eye phenotype of Rac1GMR.PN
bsk1/bsk[+] is a suppressor | partially of eye phenotype of Rab11mo
bsk1/bsk[+] is a suppressor of ommatidium phenotype of Scer\GAL4hs.2sev, nmoUAS.cUa
bsk1/bsk[+] is a suppressor of wing phenotype of flw1, pucA251.1F3/puc[+]
bsk1 is a suppressor of phenotype of flwG0172/flwG0172
bsk1/bsk[+] is a suppressor | partially of eye phenotype of Scer\GAL4hs.2sev, Tak1UAS.cMa
bsk1/bsk[+] is a suppressor | partially of microchaeta phenotype of Rala35d
bsk1/bsk[+] is a suppressor of macrochaeta phenotype of Rala35d
bsk1 is a suppressor of eye phenotype of RetMEN2B.GMR
bsk1 is a suppressor of eye phenotype of RetMEN2A.GMR
bsk1/bsk[+] is a suppressor of posterior crossvein phenotype of Scer\GAL469B, nmoc5-1.UAS
bsk1/bsk[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL469B, nmoc5-1.UAS
bsk1/bsk[+] is a suppressor | partially of ommatidium phenotype of peb1
bsk1/bsk[+] is a suppressor of ommatidium phenotype of Rac1GMR.PN
bsk1 is a suppressor of ommatidium phenotype of Rac1V12.hs.sev
bsk1/bsk[+] is a suppressor of scutum & macrochaeta phenotype of RalaS25N.UAS, Scer\GAL4sca-537.4
NOT Suppressor of
Statement
Reference
bsk1/bsk[+] is a non-suppressor of eye | somatic clone phenotype of gfr1
bsk1/bsk[+] is a non-suppressor of photoreceptor cell & axon phenotype of Scer\GAL4GMR.PF/Scer\GAL4GMR.PF, msnEP549
bsk1 is a non-suppressor of eye | ectopic phenotype of Scer\GAL4dpp.blk1, eyaUAS.cHa
bsk1 is a non-suppressor of wing phenotype of Scer\GAL4dpp.PH, eyaUAS.cHa
bsk1 is a non-suppressor of phenotype of Rho1rev220
Other
Additional Comments
Genetic Interactions
Statement
Reference
bsk1 heterozygosity suppresses the increased mitotic index in the adult midgut epithelium induced by the adulthood-only expression of UvragHMS01357 under the control of Scer\GAL4esg-NP5130 (and Gal80[ts], for the temporal control of expression).
A bsk1 heterozygous background is sufficient to ameliorate the lipid droplet accumulation displayed in sicilyE mutants. A bsk1 heterozygous background is sufficient to ameliorate the lipid droplet accumulation displayed in Aats-metFB mutants. A bsk1 heterozygous background is sufficient to ameliorate the lipid droplet accumulation displayed in MarfB mutants.
bsk1 significantly suppresses the dorsal closure defects and lethality seen in homozygous acal5 mutant embryos. One copy of acal5 does not suppress the dorsal closure defects and lethality seen in homozygous bsk1 mutant embryos.
Trpml1/+,bsk1/+ double heterozygotes show decreased number of NMJ boutons in third instar larvae compared to either Trpml1/+ or bsk1/+ single heterozygotes. RagC-DEY11726/+,bsk1/+ double heterozygotes show decreased number of NMJ boutons in third instar larvae compared to either RagC-DEY11726/+ or bsk1/+ single heterozygotes.
Retinal degeneration and light-response defects are strongly suppressed in ninaEP37H, ninaE17, bsk1/+ flies.
bsk1 partially suppresses the larval neuroblast proliferation defects seen in fzy5032 mutant clones.
A bsk1 heterozygous mutant background suppresses the actin remodelling and subsequent basolateral invasion of epithelial cells seen in flies expressing CskGD9345 in a stripe of cells at the anterior/posterior boundary of the larval wing disc under the control of Scer\GAL4ptc-559.1.
The Pknk06808 wing phenotype is enhanced by bsk1/+.
The reduced wing phenotype caused by expression of DLPEY09290 under the control of Scer\GAL4Bx-MS1096 is suppressed by bsk1.
bsk1/+ suppresses the frequency of ensheathment defects in raw134.47/raw155.27 mutants.
The rough eye phenotype caused by expression of DrefScer\UAS.cSa under the control of Scer\GAL4GMR.PS is weakly but significantly suppressed by bsk1/+.
The enlarged eye phenotype seen in mosaic animals in which the eye is homozygous for gfr1 is not modified by bsk1/+.
A bsk1/+ background suppressed the rough eye phenotype found upon expression of TgA.Scer\UAS under the control of Scer\GAL4GMR.PS.
The eye defects seen in Rab11mo homozygotes are partially suppressed by bsk1/+.
The cleft notum phenotype caused by expression of Nf-YAdsRNA.231-399.Scer\UAS.WIZ under the control of Scer\GAL4pnr-MD237 is enhanced by bsk1/+.
The wing defects seen in flw1/Y ; pucA251.1F3/+ flies are suppressed to wild type by bsk1/+.
bsk1/+ significantly suppresses the defects in left-right asymmetry of the anterior midgut that are seen in homozygous pucGS16811 embryos. Expression of bskDN.Scer\UAS under the control of Scer\GAL4Gap1-NP3392 significantly suppresses the defects in left-right asymmetry of the anterior midgut that are seen in homozygous pucGS16811 embryos. This suppression is stronger if the embryos are also carrying bsk1/+. 81% of homozygous late stage 15 embryos show left-right asymmetry of tilting of the nuclei of the circular visceral muscle (CVM), as occurs in wild-type embryos at this stage. However, 14% of the mutant embryos show no difference between the tilting of the nuclei of the CVM cells on the right and left sides of the presumptive first midgut constriction and 5% show an inversion of the normal asymmetry, with nuclei in the left-ventral region being tilted more than those in the right-ventral region.
Heterozygosity for bsk1 partially suppresses the mutant eye phenotype caused by expression of Tak1Scer\UAS.cMa under the control of Scer\GAL4hs.2sev.
Trans-heterozygotic combination between lkb1X5 and bsk1 induces a significant dorsal open phenotype in unhatched first instar larvae.
Shows no interaction with Mpk21.
Homozygosity for icsBG02577 suppresses the bsk1 'dorsal open' phenotype, reducing its incidence from approximately 60% to approximately 30%.
The frequency of dorsal holes in the cuticle seen in bsk1 embryos is partially suppressed by Sac1L2F/+. bsk1 shows no genetic interactions with Sac12107 or Sac1BG02228.
The weak dorsal-open phenotype seen in homozygous bsk1 embryos is enhanced by Cka05836/Cka05836.
Heterozygosity for bsk1 can suppress the wing-vein defects, as well as wing shape and size defects seen in Scer\GAL469B, nmoc5-1.Scer\UAS adults.
The defects in photoreceptor cell projection patterns seen in larvae overexpressing msnEP549 under the control of Scer\GAL4GMR.PF are not affected by one copy of bsk1.
Mosaic egg chambers in which the follicle cells are homozygous for kay2 in a bsk1/+ background degenerate during stage 9 and show a complete failure of posteriorwards migration of the main body follicle cells (migration of the border cells appears unaffected). Mosaic egg chambers in which the follicle cells are homozygous for kayS135103 in a bsk1/+ background degenerate during stage 9 and show a complete failure of posteriorwards migration of the main body follicle cells (migration of the border cells appears unaffected).
The proportion of eyaScer\UAS.cHa; Scer\GAL4dpp.PH flies with blistered wings (76%) is relatively unaffected by heterozygosity for bsk1 (69%), as is the proprotion with only mild wing defects 24% compared to (31%). (All data from experiments with a single, 'strong' P{UAS-eya.H} insertion; n=approximately 300 in each case).
When bsk1 fathers are crossed with Pkn06736 germline clone mothers, 76% of the embryos showed a dorsal closure phenotype, an enhancement of the dorsal closure phenotype caused by Pkn06736 germline clones alone.
bsk1/+ msn102/+ embryos often have a dorsal open phenotype, in contrast to bsk1 or msn102 single heterozygotes, which rarely show this phenotype.
The bsk1 dorsal open phenotype can be significantly rescued by heat induced expression of JraAsp.hs.sev.
Phenotype is enhanced by heat induced expression of JrabZIP.hs.sev.T:Ivir\HA1. Loss of a functional copy of aop suppresses the bsk1/Df(2L)flp147E dorsal closure phenotype.
Xenogenetic Interactions
Statement
Reference
A bsk1/+ background rescues the reduced adult eye size phenotype found in Scer\GAL4hs.2sev->Mmus\Gria1Lc.Scer\UAS flies. A hepr75 bsk1 background rescues the reduced adult eye size phenotype found in Scer\GAL4hs.2sev->Mmus\Gria1Lc.Scer\UAS flies. Quantification of the ommatidia density suggests that ~47% of spreading apoptosis in R cells can be rescued by a hepr75 bsk1 double mutant background. A bsk1 heterozygous background, in the presence of BacA\p35GMR.PH, generates additional rescue of the Scer\GAL4hs.2sev->Mmus\Gria1Lc.Scer\UAS eye phenotype compared to BacA\p35GMR.PH alone.
bsk1/+ partially suppresses the wing defects caused by expression of HIV-1\VpuScer\UAS.cLa under the control of Scer\GAL4dpp.blk1.
The ability of animals expressing Tn\neoRScer\UAS.T:Avic\GFP under the control of Scer\GAL4h.PU to survive in the presence of a concentration of G418 which is lethal to wild-type controls is partially suppressed if they are also heterozygous for bsk1.
The neuronal degeneration phenotype and decreased survival of animals expressing Hsap\HDQ93.ex1p.Scer\UAS under the control of Scer\GAL4elav-C155 is modestly suppressed in a bsk1/+ mutant background, but the effect is not statistically significant.
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments
Cuticle phenotypes reveal an allelic series: bskJ27 < bsk1 < bsk2 < bskflp147E.
External Crossreferences and Linkouts ( 3 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
UniProt/Swiss-Prot - Manually annotated and reviewed records of protein sequence and functional information
Synonyms and Secondary IDs (9)
References (81)