FB2025_01 , released February 20, 2025
Allele: Dmel\cact1
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General Information
Symbol
Dmel\cact1
Species
D. melanogaster
Name
FlyBase ID
FBal0001507
Feature type
allele
Associated gene
Dmel\cact
Associated Insertion(s)
Carried in Construct
Also Known As
cact99
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(Ashburner et al., 1999.10.12, Terracol and Lengyel, 1994, Roth et al., 1991, Tearle and Nusslein-Volhard, 1987)
Progenitor genotype
Cytology
Description
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      cact1/cact4 larvae do not show R2-R5 photoreceptor axon targeting defects in the developing visual system.

      (Minidorff et al., 2007)

      Mutant third instar larvae have melanotic nodules found in the hemocoel or in association with He-positive lymph glands. They also show gut melanisation not accompanied by tissue overgrowth or hemocyte encapsulation.

      (Minakhina and Steward, 2006)

      cact1/cact4 mutant embryos exhibit a strong ventralised phenotype.

      (Gordon et al., 2005)

      Pupal lethal when heterozygous with the MBT chromosome.

      (Riede, 2001)

      Weakly ventralised embryos. When maternal or zygotic tkv levels are reduced most of the embryos die and show an increased level of ventralisation.

      (Terracol and Lengyel, 1994)

      Strong ventralizing phenotype: lack of all dorsally and laterally derived structures and the expansion of the ventral epidermis around the entire circumference. Expansion of twi expression in terminal regions.

      (Roth et al., 1991)

      Embryos from homozygous mothers appear 'ventralized'; at differentiation they form only a narrow strip of dorsal cuticle, whereas ventral setal belts are expanded and encircle in irregular fashion most of the embryonic periphery. At gastrulation the germband hardly extends at all and the posterior midgut invaginates close to the posterior pole. Cephalic furrow is more pronounced than in wildtype.

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Enhancer of
      Statement
      Reference

      cact[+]/cact1, Scer\GAL4GMR.PF is an enhancer of increased cell death phenotype of Hsap\CHMP2BIntron5.UAS, Scer\GAL4GMR.PF

      (Ahmad et al., 2009)
      NOT Enhancer of
      Statement
      Reference

      cact[+]/cact1 is a non-enhancer of visible | heat sensitive phenotype of peb1

      (Wilk et al., 2004)
      NOT Suppressor of
      Statement
      Reference

      cact[+]/cact1 is a non-suppressor of visible | heat sensitive phenotype of peb1

      (Wilk et al., 2004)
      Other
      Statement
      Reference

      cact1, srn88 has lethal | pupal stage phenotype

      (Riede, 2001)

      cact1, efe89 has lethal | embryonic stage phenotype

      (Riede, 2001)

      Df(3R)mbtP, cact1 has lethal | pupal stage phenotype

      (Riede, 2001)
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      cact[+]/cact1, Scer\GAL4GMR.PF is an enhancer of eye phenotype of Hsap\CHMP2BIntron5.UAS, Scer\GAL4GMR.PF

      (Ahmad et al., 2009)
      NOT Enhancer of
      Statement
      Reference

      cact4/cact1 is a non-enhancer of embryo | ventral phenotype of Scer\GAL4VP16.nanos.UTR, wntDUAS.cGa

      (Gordon et al., 2005)

      cact[+]/cact1 is a non-enhancer of eye | heat sensitive phenotype of peb1

      (Wilk et al., 2004)
      NOT Suppressor of
      Statement
      Reference

      cact[+]/cact1 is a non-suppressor of eye | heat sensitive phenotype of peb1

      (Wilk et al., 2004)
      Other
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      wntDScer\UAS.cGa,Scer\GAL4nos.UTR.T:Hsim\VP16 cact4/cact1 mutant embryos exhibit a strongly dorsalised phenotype identical to wntDScer\UAS.cGa,Scer\GAL4nos.UTR.T:Hsim\VP16 alone.

      (Gordon et al., 2005)

      6% of embryos derived from cype03771/+ ; cact1/+ females crossed to wild-type males die. 80% of the dead embryos are weakly ventralised (the head is not involuted, the posterior segments are internalised and the Filzkorper are disorganised) and 20% are moderately ventralised (the ventral denticle belt is extended laterally, Filzkorper are reduced and disorganised, the head is not involuted and the head and thorax are extended and convoluted).

      (Szuplewski and Terracol, 2001)
      Xenogenetic Interactions
      Statement
      Reference

      The presence of a copy of cact1 significantly enhances the Hsap\CHMP2BIntron5.Scer\UAS Scer\GAL4GMR.PF phenotype.

      (Ahmad et al., 2009)
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (2)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      References (22)