Open Close
General Information
Symbol
Dmel\capu1
Species
D. melanogaster
Name
FlyBase ID
FBal0001537
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
capuRK12, capuRK, cappuccinoRK
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

T3873840A

Amino acid change:

L768Q | capu-PA; L758Q | capu-PB; L916Q | capu-PD; L989Q | capu-PE; L1070Q | capu-PF; L641Q | capu-PG; L816Q | capu-PH; L1007Q | capu-PI; L798Q | capu-PJ

Reported amino acid change:
Comment:

Site of nucleic acid difference inferred by FlyBase curator based on reported amino acid change; CDS from reference sequence longer by 1 amino acid than published CDS in GB:U34258 and thus the mutation coordinates differ by 1; a sequence difference between the reference sequence and the published sequence account for the L->Q versus L->H change predicted for this mutation

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: L767H.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Scer\GAL4nos.UTR.T:Hsim\VP16>capuScer\UAS.P\T.RA.T:Avic\GFP expression rescues the capu1 mutant background in that the mesh is present and streaming does not begin prematurely in 100% of egg chambers. 36% of the eggs laid by the capu1 mutant females expressing capuScer\UAS.P\T.RA.T:Avic\GFP hatch.

56% of eggs laid by capu1 mutant females expressing Scer\GAL4nos.UTR.T:Hsim\VP16>capuScer\UAS.P\T.RA hatch.

18% of eggs laid by capu1 mutant females expressing Scer\GAL4nos.UTR.T:Hsim\VP16>capuL1048A.Scer\UAS.P\T.RA.T:Avic\GFP hatch.

capu1 mutant females expressing Scer\GAL4nos.UTR.T:Hsim\VP16>capuI706A.Scer\UAS.P\T.RA.T:Avic\GFP are sterile. None of their eggs hatch. No actin mesh is detectable in the mutant oocytes and streaming starts prematurely.

capu2L-281-6/capu1 females produce dorsalised eggs.

Stage 10 homozygous egg chambers contain binucleate cells. These binucleate cells are not detectable in the germarium or early stages of oogenesis. Fragments of ring canals are seen.

The oocyte nucleus is still located in an anterior position in a stage 8 egg chamber in homozygous females.

40-70% of embryos laid by homozygous females have abnormal eggshells. Abnormal microtubule distribution in stage 8 and 9 egg chambers (distribution resembles that of stage 10 egg chambers), long and thick tubulin fibres are seen wrapping around the cortex of mutant oocytes. Egg chambers undergo premature microtubule based cytoplasmic streaming.

Dorsoventral and anteroposterior patterning is disrupted.

Variable expansion (dorsalization) of dorsal appendages, at expense of main body shell. Absence of polar granules, pole cells and abdominal segments in embryos.

Homozygous embryos derived from homozygous females have no polar granules, fail to form pole cells, have deletions of abdominal structures and defects associated with dorsal/ventral pattern formation.

Homozygous mothers produce dorsalized egg shells and embryos. The first sign of dorsalization in embryos can be seen during gastrulation, when the ventral furrow is reduced or absent. Dorsalization in the egg chamber is evident in the shape of the follicle cells. While the number of follicle cells around the main body of the egg shell is reduced, dorsal appendages are expanded, often fused dorsally and/or extended ventrally. The embryos lack polar granules and pole cells, and show cellularization defects. Embryos show strong abdominal segmentation defects similar to those produced by mutations in the grandchildless-knirps or posterior class of maternal effect loci, abdominal segments are deleted.

Homozygous femles lay eggs which sometimes (5-10%) have a 'pointed cap' (cappuccino) of dorsal appendage material sitting over the anterior end of the egg, instead of two distinct dorsal appendages. Such eggs are similar to eggs formed by the female-sterile mutation fs(1)K10 but the extent of dorsal appendage material on capu eggs is much more variable than that of fs(1)K10 eggs. No polar granules are found in such eggs. Mutant females produce embryos lacking polar granules, pole cells and normal abdominal segmentation. In combination with BicD, however, abdominal segmentation does develop in the anterior half of the embryo. Improper localization of abdominal determinants also indicated by the lack of posterior localization of vasa protein. Cellularization of the blastoderm irregularly defective, with nuclei of different sizes and densities. Resemble embryos formed by other grandchildless-knirps-like mutations, such as vas or tud, but in addition, some of the embryos from capu1 also appear dorsalized. Mosaic studies demonstrate germ-line function of capu.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Other
Phenotype Manifest In
Enhancer of
Statement
Reference

capu[+]/capu1 is an enhancer of egg phenotype of Ets97Dtne-1

NOT Enhancer of
Statement
Reference

capu[+]/capu1 is a non-enhancer of mitotic domain 1 | embryonic cycle 14 phenotype of CycB+t10

NOT Suppressor of
Statement
Reference

capu[+]/capu1 is a non-suppressor of mitotic domain 1 | embryonic cycle 14 phenotype of CycB+t10

Other
Additional Comments
Genetic Interactions
Statement
Reference

Stage 8 oocytes from capu1; Rho11B and capu1/+, spir2F/+ mothers exhibit premature ooplasmic streaming and subcortical arrays of microtubules. Stable microtubules are restricted to the cortex, as in wild-type oocytes, but differ in the lack of reduction of microtubules at the posterior pole.

ctC145/+ ; capu1/+ double heterozygotes produce egg chambers with binucleate cells. The majority of egg chambers contain a single binucleate cell, although occasionally more than one binucleate cell per egg chamber is seen. The binucleate cells can occur anywhere among the nurse cells and contain remnants of ring canals. The binucleate cells are not seen prior to stage 5.

Egg chambers transheterozygous for Rho1rev220 and capu mutants show disruptions of the ovarian cytoskeleton and ring canals similar to that seen in females heterozygous for Rho1rev220 and RpII140wimp.

One copy of capu1 decreases the number of eggs laid by Ets97Dtne-1/Df(3R)ro80b females.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

capu1 is partially rescued by capuUASp.RA.GFP

capu1 is partially rescued by capuUASp.cQa

capu1 is partially rescued by capuL1048A.UASp.RA.GFP

Comments

Scer\GAL4nos.UTR.T:Hsim\VP16>capuScer\UAS.P\T.RA.T:Avic\GFP expression rescues the capu1 mutant background in that the mesh is present and streaming does not begin prematurely in 100% of egg chambers. 36% of the eggs laid by the capu1 mutant females expressing capuScer\UAS.P\T.RA.T:Avic\GFP hatch.

56% of eggs laid by capu1 mutant females expressing Scer\GAL4nos.UTR.T:Hsim\VP16>capuScer\UAS.P\T.RA hatch.

18% of eggs laid by capu1 mutant females expressing Scer\GAL4nos.UTR.T:Hsim\VP16>capuL1048A.Scer\UAS.P\T.RA.T:Avic\GFP hatch.

capu1 mutant females expressing Scer\GAL4nos.UTR.T:Hsim\VP16>capuI706A.Scer\UAS.P\T.RA.T:Avic\GFP are sterile. None of their eggs hatch. No actin mesh is detectable in the mutant oocytes and streaming starts prematurely.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Posterior localization of vas and CycB transcripts is completely abolished.

Localization of vas protein to pole cells and polar granules abolished.

Moderate capu allele.

Prevents the posterior accumulation of G-iα65A protein.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (36)