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General Information
Symbol
Dmel\car1
Species
D. melanogaster
Name
FlyBase ID
FBal0001541
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
carnation1
Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C19566417G

Amino acid change:

L26V | car-PA; L26V | car-PB; L26V | car-PC

Reported amino acid change:

L26V

Comment:

One of two amino acid substitutions observed in car1 mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

point mutation
Nucleotide change:

G19567087T

Amino acid change:

G249V | car-PA; G249V | car-PB; G249V | car-PC

Reported amino acid change:

G249V

Comment:

One of two amino acid substitutions observed in car1 mutant. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Mutated residue Gly249 is a conserved residue in car homologues of different species.

Amino acid replacement: L26V.

Amino acid replacement: G249V.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

car1 homozygotes or heterozygotes do not exhibit a significant increase in the proportion of hyperplastic testes, as compared to controls.

car1 mutant males exhibit ~40% less spermatogonial cyst death compared to controls.

car1 mutants exhibit an endosome clustering phenotype.

car1 mutants exhibit retinal degeneration after 9-days of constant light.

Both number and morphology of LysoTracker-positive puncta and lipid droplets during ecdysone-induced autophagy in car1 male wandering third instar larvae is comparable to wild-type.

Mutant flies show some decrease in ommochrome pigment levels and a significant reduction in drosopterin pigment levels compared to wild type.

Mutant males have a shortened adult life span compared to controls, with a mean life span of 17.42 days at 25o.

Hemocyte endosomal degradation is significantly reduced in car1 mutants compared to wild-type cells. This reduction also correlates with an increased severity of eye-colour defect. car1 mutants show a small but significant impairment in endosomal degradation. There is no difference between in the morphology and formation of the multi-vesicular body and the small dense organelle between car1 and wild-type cells. Cells from car1 animals exhibit a block in transition of small dense vesicles to tubular-vesicular compartments. Endosomes in car1 mutant cells exhibit wild-type fusion characteristics and mature into fusion-inaccessible small dense structures.

Brain histology abnormal.

Malpighian tubule colour: pale yellow; hard to distinguish from wild type before third instar.

Eye color autonomous in transplant into wild-type host.

Eye colour: dark ruby. Body shape and proportions seem rounded.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

car1 is a suppressor of small body | pupal stage phenotype of Nf1E2

Other
Phenotype Manifest In
Suppressed by
Statement
Reference

car1 has eye phenotype, suppressible by CysGMR.PK

car1 has ommatidium phenotype, suppressible by CysGMR.PK

car1 has rhabdomere phenotype, suppressible by CysGMR.PK

NOT suppressed by
Statement
Reference

car1 has phenotype, non-suppressible by su(Hw)2

Enhancer of
Statement
Reference
Suppressor of
Statement
Reference

car1/car1 is a suppressor of phenotype of l(2)gd1d7

car1 is a suppressor of eye phenotype of Scer\GAL4GMR.PF, Sk2UAS.cYa

Other
Additional Comments
Genetic Interactions
Statement
Reference

car1/car1, p53E8/+ adults do not exhibit a significant increase in the proportion of hyperplastic testes, as compared to car1/car1 adults, p53E8/+ adults, or wild-type controls.

Homozygosity of car1 suppresses the ectopic N activation phenotype (ectopic expression of wg) in l(2)gd1d7 wing discs.

Expression of rushScer\UAS.T:Avic\GFP under the control of Scer\GAL4arm.PS decreases the fraction of car1 flies eclosing. This effect is rescued by Df(1)rush4, sta+t4.4.

The presence of CysGMR rescues the retinal degeneration seen in car1 after 9-days of constant light.

The reduction in drosopterin pigment levels that is seen in car1 flies is not affected by HPS4W515X.

The reduction in drosopterin pigment levels seen in flies carrying wdsRNA.GMR is not enhanced by car1.

A car1 mutant background suppresses the retinal degeneration seen upon expression of Sk2Scer\UAS.cYa under the control of Scer\GAL4GMR.PF in the developing eye through enhanced lysosomal degradation.

Eye colour: car1; pp eyes are lighter than either single mutant. The levels of red pigment in the double mutants is comparable to that in car1 single mutant eyes and the level of brown pigment is comparable to pp single mutant eyes.

The double mutant, car1 dor1 does not survive beyond the prepupal stage. In cell culture, car1 dor1 cells exhibit the most severe defect in endosomal degradation, compared to either single mutant. There is a small but significant difference between the degradation defect in dor1 car1 and dor1, consistent with a role for both car and dor in endosomal degradation. Endosomes in cells from car1 dor1 mutants, although incapable of a morphological transition to small dense vesicles, are capable of fusion at early times and normal maturation. The presence of Dp(1;Y)1E in car1 mutants blocks the transition from large to small endosomes during maturation.

Lethal focus of the lt car lethal interaction as measured in car1/+; lt1/lt1, car1/0; lt1/lt1 gynandromorphs is in ventral nervous tissue.

Dies as larva when mother car1; ltunspecified/+ or car1/+; ltunspecified and as pupa when mother car1/+; ltunspecified/+.

Eye colour: brownish yellow to brown with bw1. Eye colour: yellow-brown with st1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (2)
Stocks (45)
Notes on Origin
Discoverer
Comments
Comments

Reduced recovery in gynandromorphs with male parts car dor.

Lethal focus domineering; fate maps to ventral nervous system.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (28)