L26V | car-PA; L26V | car-PB; L26V | car-PC
G249V | car-PA; G249V | car-PB; G249V | car-PC
Eye colour: car1; pp eyes are lighter than either single mutant. The levels of red pigment in the double mutants is comparable to that in car1 single mutant eyes and the level of brown pigment is comparable to pp single mutant eyes.
The double mutant, car1 dor1 does not survive beyond the prepupal stage. In cell culture, car1 dor1 cells exhibit the most severe defect in endosomal degradation, compared to either single mutant. There is a small but significant difference between the degradation defect in dor1 car1 and dor1, consistent with a role for both car and dor in endosomal degradation. Endosomes in cells from car1 dor1 mutants, although incapable of a morphological transition to small dense vesicles, are capable of fusion at early times and normal maturation. The presence of Dp(1;Y)1E in car1 mutants blocks the transition from large to small endosomes during maturation.