FlyBase Allele Report: Dmel\Cdk1[E1-23]

General Information

Symbol

Dmel\Cdk1^E1-23

Species

D. melanogaster

Name

FlyBase ID

FBal0001573

Feature type

allele

Associated gene

Dmel\Cdk1

Associated Insertion(s)

Carried in Construct

Also Known As

cdc2^E1-23

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Tio et al., 2001, Sigrist et al., 1995, Sigrist et al., 1995, Clegg et al., 1993, Stern et al., 1993)

Mutagen

ethyl methanesulfonate

(Clegg et al., 1993, Stern et al., 1993)

Progenitor genotype

Cytology

Description

Amino acid replacement: G206D. Nucleotide substitution: G?A.

(Clegg et al., 1993)

Missense mutation: glycine replaced by aspartic acid in a position that is completely invariant in known cdc2 homologs.

(Stern et al., 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

2L:10,385,176..10,385,176 [-]

Nucleotide change:

G10385176A

Reported nucleotide change:

G?A

Amino acid change:

G206D | Cdk1-PA

Reported amino acid change:

G206D

Comment:

The Cdk1^E1-23 sequence is in GB:S66807.

(Clegg et al., 1993, Stern et al., 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

S66807

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

axon | larval stage (with Cdk1^B47)

(Li et al., 2021)

imaginal disc

(Stern et al., 1993)

imaginal disc | larval stage

(Clegg et al., 1993)

larva

(Stern et al., 1993)

larval brain

(Clegg et al., 1993)

larval multidendritic class IV neuron (with Cdk1^B47)

(Li et al., 2021)

Detailed Description

Statement

Reference

Cdk1^B47/Cdk1^E1-23 show decreased class IV da neuron axonal regeneration.

(Li et al., 2021)

Lethal phase is during the larval/pupal stage. Homozygous embryos develop essentially normally. RP2 neuron duplication and large RP2 neurons are seen in only 0.25% and 0.8% of cases respectively.

(Tio et al., 2001)

Larvae contain no imaginal discs. Brain development is abnormal.

(Clegg et al., 1993)

Die after pupariation at the larval-pupal interface. Larvae take 1-2 days longer than wild type to reach the wandering stage. Imaginal discs are missing or rudimentary and larval brains are reduced in size. Endoreplication and polytenization of larval cells is normal, as are salivary gland size and intensity of DNA staining of their polytene nuclei.

(Stern et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

cdc2[+]/Cdk1^E1-23, CycD¹ has viable phenotype

(Emmerich et al., 2004)

Cdk1^E1-23, CycD¹ has viable phenotype

(Emmerich et al., 2004)

Phenotype Manifest In

Enhanced by

Statement

Reference

Cdk1^E1-23 has larval cuticle & larval abdomen phenotype, enhanceable by Cks30A^HG24/Cks30A^RA74

(Swan et al., 2005)

Enhancer of

Statement

Reference

Cdk1^E1-23 is an enhancer of larval cuticle & larval abdomen phenotype of Cks30A^HG24/Cks30A^RA74

(Swan et al., 2005)

Additional Comments

Genetic Interactions

Statement

Reference

Cks30A^RA74/Cks30A^HG24 mutants, in combination with cdc2^E1-23 exhibit very strong abdominal cuticle defects.

(Swan et al., 2005)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

Cdk1^E1-23/Df(2L)J27 is partially rescued by Cdk1^P131S

(Sigrist et al., 1995)

Cdk1^E1-23/Df(2L)J27 is partially rescued by Cdk1^A171T

(Sigrist et al., 1995)

Cdk1^E1-23/Df(2L)J27 is partially rescued by Cdk1^G43E

(Sigrist et al., 1995)

Not rescued by

Cdk1^E1-23/Df(2L)J27 is not rescued by Cdk1^G177E

(Sigrist et al., 1995)

Comments

Lethality of heterozygotes with Df(2L)J27 can be partially rescued by expression of cdc2^A171T, cdc2^G43E or cdc2^P131S at 18^oC, levels of rescue drop at higher temperatures.

(Sigrist et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6629

Cdk1^E1-23 Adc^b-1 pr¹ cn¹/CyO

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 1 )

Crossreferences

GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.

S66807

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Cdk1^E1-23

(Li et al., 2021, Liaw and Chiang, 2019, Swan et al., 2005, Emmerich et al., 2004)

Dmcdc2^E1-23

(Sigrist et al., 1995)

cdc2^E1-23

(Wang and Baker, 2015, Chen et al., 2008, Christensen and Cook, 2007.10.29, Christensen and Cook, 2006.8.30)

cdk1^E1-23

(Deichsel et al., 2024, Deichsel et al., 2024, Baumgardt et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (16)

Report Sections

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