Allele Dmel\ck¹³

General Information
Symbol	Dmel\ck¹³	Species	D. melanogaster
Name		FlyBase ID	FBal0001693
Feature type	allele	Created / Updated	2006-08-22/2006-08-22
Associated gene	Dmel\ck

Allele class
Mutagen	ethyl methanesulfonate
Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (O'Donnell et al., 1977, Gubb et al., 1984, Ashburner et al., 1999.10.12)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: R768@. (Kiehart et al., 2004)
Assay mode
Cytology	Polytene chromosomes normal. (Gubb et al., 1984)
Phenotypic Data
Phenotypic Class
	lethal \| recessive (O'Donnell et al., 1977, Gubb et al., 1984) lethal \| embryonic stage \| recessive (Kiehart et al., 2004) semi-lethal \| recessive sterile \| recessive (Kiehart et al., 2004) auditory system defective (Todi et al., 2005) auditory system defective (with ck⁰⁷¹³⁰) (Todi et al., 2005) auditory system defective (with ck⁰⁷¹³⁰), with Scer\GAL4^elav-C155, ck^{tail.Scer\UAS.T:Avic\GFP} (Todi et al., 2005) auditory system defective (with ck⁰⁷¹³⁰), with Scer\GAL4^sqh.PW, ck^{tail.Scer\UAS.T:Avic\GFP} (Todi et al., 2005) auditory system defective, with Scer\GAL4^elav-C155, ck⁰⁷¹³⁰, ck^{tail.Scer\UAS.T:Avic\GFP} (Todi et al., 2005) auditory system defective, with Scer\GAL4^sqh.PW, ck⁰⁷¹³⁰, ck^{tail.Scer\UAS.T:Avic\GFP} (Todi et al., 2005)
Phenotype Manifest In
	Johnston's organ & scolopidium (with ck⁰⁷¹³⁰) (Todi et al., 2005) Johnston's organ & dendritic cap cell (with ck⁰⁷¹³⁰) (Todi et al., 2005) wing hair \| ectopic (Kiehart et al., 2004) arista (Kiehart et al., 2004) macrochaeta (Kiehart et al., 2004) microchaeta (Kiehart et al., 2004) trichogen cell (Kiehart et al., 2004) wing hair (Gubb et al., 1984) Johnston's organ & scolopidium (Todi et al., 2005) Johnston's organ & dendritic cap cell (Todi et al., 2005)
Detailed Description
	Statement Reference Nearly all homozygotes die as embryos. Mutant escapers are not fertile. Hemizygous escapers have a number of defects, including deep ridges in and aberrant projections from the shaft of the macrochaetae on the thorax. Both macrochaetae and microchaetae are stubby, branched, frequently twisted and not uniformly or smoothly tapered. Aristae are shorter and more highly branched than wild type. Wing hairs show a multiple wing hair phenotype. (Kiehart et al., 2004) Class 2 (survival at >1% <5% expected). Hemizygotes escape lethality with a frequency of 1%-2%. Escapers exhibit multiple trichomes on the wing. Escapers show two discrete periods of death, half as larvae and the remainder pupariate and develop into pharate adults, most die in the pupal case, only a few eclose. (Gubb et al., 1984) ck¹³ flies show no response to the pulse song, or to sine frequencies up to 1000 Hz, as measured via sound-evoked potentials from the antennal nerve. ck¹³/ck⁰⁷¹³⁰ transheterozygotes show a significantly reduced response to the pulse song compared to ck⁰⁷¹³⁰/+ controls. The Johnston's organ located at antennal segment 2 shows overall disorganization in ck¹³ mutants, with apical detachment of the scolopidia. The dendritic cap of the scolopidium often fails to enclose one of the cilia and may is sometimes divided into several compartments. However, scolopidial basal attachments to the a2 cuticle are not affected and a2/a3 joint epithelial cells are present, showing that detachment is not due to their degeneration. This phenotype is also observed in ck¹³/ck⁰⁷¹³⁰ transheterozygotes, although to a lesser extent. Expression of ck^{tail.Scer\UAS.T:Avic\GFP} in scolopale cells driven by Scer\GAL4^sqh.PW, or in neurons driven by Scer\GAL4^elav-C155, leads to a reduction in sound-evoked potentials from ck¹³/ck⁰⁷¹³⁰ flies. (Todi et al., 2005) ck¹³ flies are able to exclude markers from the cap cell-scolopale cell junction to the same extent as wild-type flies. (Todi, 2005)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference ck[+]/ck¹³ is a suppressor of visible \| conditional ts phenotype of fz^I.hs (Winter et al., 2001) ck¹³ is a suppressor of visible \| recessive phenotype of dsh¹ (Winter et al., 2001) ck[+]/ck¹³ is a suppressor \| partially of visible phenotype of Scer\GAL4^hs.PB, tow^Scer\UAS.cCa (Chung et al., 2007)
Phenotype Manifest In
Enhancer of
Statement Reference ck[+]/ck¹³ is an enhancer of eye phenotype of Rac1^GMR.PN (Tan et al., 2003)
Suppressor of
Statement Reference ck[+]/ck¹³ is a suppressor of wing hair \| supernumerary phenotype of fz^I.hs (Winter et al., 2001) ck¹³ is a suppressor of wing hair \| supernumerary phenotype of dsh¹ (Winter et al., 2001) ck[+]/ck¹³ is a suppressor \| partially of wing hair \| supernumerary phenotype of Scer\GAL4^hs.PB, tow^Scer\UAS.cCa (Chung et al., 2007)
Additional Comments
Genetic Interactions
Statement Reference The small, rough eye phenotype of Rac1^GMR.PN flies is enhanced by heterozygosity for ck¹³. (Tan et al., 2003) S^48-5/ck¹³ mutants show 5.41%+-2.22 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone. (Gaengel and Mlodzik, 2003) The polarity of hairs surrounding ck[13], d[GC13] clones in the abdomen appear normal. (Mao et al., 2006) The multiple wing hair phenotype caused by expression of tow[Scer\UAS.cCa] under the control of Scer\GAL4[hs.PB] using heat shock at 24 hours after puparium formation is partially suppressed by one copy of ck[13]. (Chung et al., 2007)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	ck¹³ is rescued by ck^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^sqh.PW (Todi et al., 2005)
Partially rescued by	ck¹³ is partially rescued by ck^{Scer\UAS.T:Avic\GFP}/Scer\GAL4^nompA.cK (Todi et al., 2005) ck¹³ is partially rescued by Scer\GAL4^elav-C155/ck^{Scer\UAS.T:Avic\GFP} (Todi et al., 2005)
Comments	Expression of ck^{Scer\UAS.T:Avic\GFP} in the scolopale cells, driven by Scer\GAL4^nompA.cK, or in neurons, driven by Scer\GAL4^elav-C155, leads to a significant partial rescue of the ck¹³ auditory phenotype. (Todi et al., 2005)
Stocks ( 8 )
Kyoto	101563 wg^l-12 ck¹³ pr¹ cn¹ / CyO 108737 y¹; Dp(1;2)sc¹⁹, ck¹³ pr¹ pwn¹/CyO 108128 y¹; P{Car20y}25F ck¹³ P{neoFRT}40A/CyO; kar² ry⁵⁰⁶ 102016 f^36a ; P{f+13}30B ck¹³ pr¹ pwn¹ / CyO ; mwh¹ jv¹ 101288 Adh^D ck¹³ pr¹ cn¹ / CyO, Cy¹ dp^lv1 Adh^nB pr¹ cn²
Bloomington	2202 f^36a; P{f⁺13}30B ck¹³ pr¹ pwn¹/CyO 6329 y¹; Dp(1;2)sc¹⁹, ck¹³ pr¹ pwn¹/CyO 5259 y¹; P{Car20y}25F ck¹³ P{neoFRT}40A/CyO; kar² ry⁵⁰⁶
Notes on Origin
Discoverer

Comments
	Phenotypic data included in FBrf0051973. (Roote, 1995.5.4)
Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	CH-52 (O'Donnell et al., 1977) CH52 ck-13 (Gaengel and Mlodzik, 2003) ck¹³ (Mao et al., 2006, Christensen and Cook, 2007.5.8, Christensen and Cook, 2007.3.22, Chung et al., 2007) ck^CH52 (Gubb et al., 1984) ck^P13 (Tan et al., 2003) l(2)br27^CH52 (Ashburner, 1982) stch (Baker, 2006.4.24)
Name Synonym
Secondary FlyBase IDs

References ( 16 )
Research paper	Chung et al., 2007, Genetics 176(2): 891--903 The balance between the novel protein target of wingless and the Drosophila Rho-associated kinase pathway regulates planar cell polarity in the Drosophila wing. [FBrf0202398] Mao et al., 2006, Development 133(13): 2539--2551 Dachs: an unconventional myosin that functions downstream of Fat to regulate growth, affinity and gene expression in Drosophila. [FBrf0193425] Todi et al., 2005, Curr. Biol. 15(9): 862--868 Myosin VIIA defects, which underlie the Usher 1B syndrome in humans, lead to deafness in Drosophila. [FBrf0187338] Kiehart et al., 2004, Genetics 168(3): 1337--1352 Drosophila crinkled, mutations of which disrupt morphogenesis and cause lethality, encodes fly myosin VIIA. [FBrf0180276] Gaengel and Mlodzik, 2003, Development 130(22): 5413--5423 Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6. [FBrf0167499] Tan et al., 2003, Development 130(4): 671--681 Roles of myosin phosphatase during Drosophila development. [FBrf0155719] Winter et al., 2001, Cell 105(1): 81--91 Drosophila Rho-associated kinase (Drok) links Frizzled-mediated planar cell polarity signaling to the actin cytoskeleton. [FBrf0135688] Gubb et al., 1984, Chromosoma 91: 54--64 The genetic analysis of a large transposing element of Drosophila melanogaster. The insertion of a w<up>+</up> rst<up>+</up> TE into the ck locus. [FBrf0040529] Ashburner, 1982, Genetics 101: 447--459 The genetics of a small autosomal region of Drosophila melanogaster containing the structural gene for Alcohol dehydrogenase. [FBrf0038053] O'Donnell et al., 1977, Genetics 86: 553--566 Genetic and cytogenetic analysis of the Adh region in Drosophila melanogaster. [FBrf0030161]
Supplementary material	Todi, 2005, Curr. Biol. 15(9): [title not yet available] [FBrf0191736]
Personal communication to FlyBase	Christensen and Cook, 2007.5.8, Isolation and characterization of Df(2L)BSC299. Isolation and characterization of Df(2L)BSC299. [FBrf0198999] Christensen and Cook, 2007.3.22, Isolation and characterization of Df(2L)BSC254. Isolation and characterization of Df(2L)BSC254. [FBrf0199294] Baker, 2006.4.24, [title not yet available] [title not yet available] [FBrf0191693] Ashburner et al., 1999.10.12, Adh. Adh. [FBrf0112153] Roote, 1995.5.4, Genetics 126: 679 help please. Genetics 126: 679 help please. [FBrf0079819]

Allele Dmel\ck13

Allele Dmel\ck¹³