FlyBase Allele Report: Dmel\cno[2]

General Information

Symbol

Dmel\cno²

Species

D. melanogaster

Name

FlyBase ID

FBal0001748

Feature type

allele

Associated gene

Dmel\cno

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Tearle and Nusslein-Volhard, 1987)

Progenitor genotype

Cytology

Description

Amino acid replacement: Q1310term.

(Gurley et al., 2023)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:5,176,178..5,176,178 [-]

Nucleotide change:

C5176178T

Amino acid change:

Reported amino acid change:

Q1310term

Comment:

Site and nature of nucleotide substitution in mutant inferred by FlyBase curator based on reported amino acid change.

(Gurley et al., 2023)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

cuticle

(Gurley et al., 2023)

ectoderm | embryonic stage

(Boettner and Van Aelst, 2007)

ectoderm | embryonic stage (with cno^mis1)

(Boettner and Van Aelst, 2007)

embryo

(Gurley et al., 2023, Boettner and Van Aelst, 2007)

embryo (with cno^mis1)

(Boettner and Van Aelst, 2007)

embryo | dorsal closure stage

(Boettner et al., 2003)

embryo | dorsal closure stage (with cno^mis1)

(Boettner et al., 2003)

embryo | embryonic stage 14 (with cno^mis1)

(Boettner et al., 2003)

embryonic/first instar larval cuticle | embryonic stage

(Boettner and Van Aelst, 2007)

embryonic/first instar larval cuticle | embryonic stage (with cno^mis1)

(Boettner and Van Aelst, 2007)

eye

(Miyamoto et al., 1995)

eye photoreceptor cell (with cno^mis1)

(Chen et al., 2000)

ommatidium (with cno^mis1)

(Fetting et al., 2009, Gaengel and Mlodzik, 2003, Chen et al., 2000)

Detailed Description

Statement

Reference

cno²/+ heterozygous embryos do not display significant increase in the number of hemisegments with abnormal number of neurons in the RP2 lineage compared to wild-type.

(Keder et al., 2015)

Many cno²/cno^mis1 ommatidia mis-rotate; the variance (s.d.) in degree of rotation is greater than in wild type in adult eyes. Most of the ommatidia over rotate.

(Fetting et al., 2009)

54% of cno²/cno^mis1 embryos hatch, 23% have their cuticle closed dorsally (with 16% having a head involution defect), 22% have the cuticle partially open and 1% have a large dorsal hole in the cuticle (covering at least half of the dorsal aspect). cno² embryos show defects ranging from small anterior holes in the cuticle (19%) to large holes covering almost the entire dorsal aspect of the embryo (81%). The leading edge cytoskeleton is assembled largely as in wild-type embryos and an initial stretching of the lateral ectodermal cells takes place during the dorsal closure stage in cno² embryos. However, at later stages of dorsal closure, the mutant embryos show a detachment of the lateral ectoderm from the amnioserosa; the ectoderm retracts, with the cells resuming a non-elongated shape and the amnioserosa shrivels.

(Boettner et al., 2003)

cno^mis1/cno² animals exhibit an ommatidial rotation phenotype in the adult eye which is also seen in third instar eye imaginal discs.

(Gaengel and Mlodzik, 2003)

cno^mis1/cno² flies have mostly normal facets in the eye, with a small proportion of facets missing photoreceptors.

(Chen et al., 2000)

Transheterozygotes cno^mis1/cno² have a roughened eye structure.

(Miyamoto et al., 1995)

strongest allele

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

cno^mis1/cno² has increased mortality during development phenotype, enhanceable by PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has abnormal cell migration | embryonic stage phenotype, enhanceable by PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has increased mortality during development phenotype, enhanceable by zip²/zip^Ebr

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has abnormal cell migration | embryonic stage phenotype, enhanceable by zip²/zip^Ebr

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has increased mortality during development phenotype, enhanceable by PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has abnormal cell migration | embryonic stage phenotype, enhanceable by PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has some die during embryonic stage phenotype, enhanceable by Rap1^CD5/Rap1^CD5

(Boettner et al., 2003)

NOT Enhanced by

Statement

Reference

cno² has abnormal cell migration | embryonic stage phenotype, non-enhanceable by Scer\GAL4^VP16.PDZ-GEF/PDZ-GEF^UAS.cBa

(Boettner and Van Aelst, 2007)

cno² has abnormal cell migration | embryonic stage phenotype, non-enhanceable by PDZ-GEF^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^VP16.PDZ-GEF

(Boettner and Van Aelst, 2007)

Suppressed by

Statement

Reference

cno² has lethal | embryonic stage phenotype, suppressible by Scer\GAL4^ptc-559.1/bsk^UAS.cBa

(Boettner et al., 2003)

NOT suppressed by

Statement

Reference

cno² has lethal - all die during embryonic stage phenotype, non-suppressible by Scer\GAL4^VP16.PDZ-GEF/PDZ-GEF^UAS.cBa

(Boettner and Van Aelst, 2007)

cno² has lethal - all die during embryonic stage phenotype, non-suppressible by PDZ-GEF^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^VP16.PDZ-GEF

(Boettner and Van Aelst, 2007)

cno² has abnormal cell migration | embryonic stage phenotype, non-suppressible by Scer\GAL4^VP16.PDZ-GEF/PDZ-GEF^UAS.cBa

(Boettner and Van Aelst, 2007)

cno² has abnormal cell migration | embryonic stage phenotype, non-suppressible by PDZ-GEF^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^VP16.PDZ-GEF

(Boettner and Van Aelst, 2007)

cno² has lethal | embryonic stage phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Rap1^{V12.UAS.Tag:MYC}

(Boettner et al., 2003)

Enhancer of

Statement

Reference

cno^mis1/cno² is an enhancer of increased mortality during development phenotype of PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² is an enhancer of increased mortality during development phenotype of PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² is an enhancer of increased mortality during development phenotype of zip²/zip^Ebr

(Boettner and Van Aelst, 2007)

cno² is an enhancer of abnormal cell polarity phenotype of S^48-5

(Gaengel and Mlodzik, 2003)

Other

Statement

Reference

cno², wts^x1/wts[+] has abnormal neuroanatomy | embryonic stage phenotype

(Keder et al., 2015)

cno², ex¹/ex[+] has abnormal neuroanatomy | embryonic stage phenotype

(Keder et al., 2015)

Mer[+]/Mer⁴, cno² has abnormal neuroanatomy | embryonic stage phenotype

(Keder et al., 2015)

cno², mats^e03077/mats[+] has abnormal neuroanatomy | embryonic stage phenotype

(Keder et al., 2015)

cno², sav[+]/sav³ has abnormal neuroanatomy | embryonic stage phenotype

(Keder et al., 2015)

cno², yki[+]/yki^B5 has abnormal neuroanatomy | embryonic stage phenotype

(Keder et al., 2015)

cno², ft^G-rv/ft[+] has abnormal neuroanatomy | embryonic stage phenotype

(Keder et al., 2015)

cno^mis1/cno², zip^Ebr has visible | adult stage phenotype

(Boettner and Van Aelst, 2007)

Phenotype Manifest In

Enhanced by

Statement

Reference

cno^mis1/cno² has ommatidium phenotype, enhanceable by ed[+]/ed^1X5

(Fetting et al., 2009)

cno^mis1/cno² has ectoderm | embryonic stage phenotype, enhanceable by PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryo phenotype, enhanceable by PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has ectoderm | embryonic stage phenotype, enhanceable by PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryonic/first instar larval cuticle | embryonic stage phenotype, enhanceable by PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryonic/first instar larval cuticle | embryonic stage phenotype, enhanceable by zip²/zip^Ebr

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has ectoderm | embryonic stage phenotype, enhanceable by zip²/zip^Ebr

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryo phenotype, enhanceable by PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryonic/first instar larval cuticle | embryonic stage phenotype, enhanceable by PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has embryo | dorsal closure stage phenotype, enhanceable by Rap1^CD5/Rap1^CD5

(Boettner et al., 2003)

NOT Enhanced by

Statement

Reference

cno² has embryo phenotype, non-enhanceable by Scer\GAL4^VP16.PDZ-GEF/PDZ-GEF^UAS.cBa

(Boettner and Van Aelst, 2007)

cno² has embryonic/first instar larval cuticle | embryonic stage phenotype, non-enhanceable by Scer\GAL4^VP16.PDZ-GEF/PDZ-GEF^UAS.cBa

(Boettner and Van Aelst, 2007)

cno² has ectoderm | embryonic stage phenotype, non-enhanceable by Scer\GAL4^VP16.PDZ-GEF/PDZ-GEF^UAS.cBa

(Boettner and Van Aelst, 2007)

cno² has embryo phenotype, non-enhanceable by PDZ-GEF^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^VP16.PDZ-GEF

(Boettner and Van Aelst, 2007)

cno² has embryonic/first instar larval cuticle | embryonic stage phenotype, non-enhanceable by PDZ-GEF^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^VP16.PDZ-GEF

(Boettner and Van Aelst, 2007)

cno² has ectoderm | embryonic stage phenotype, non-enhanceable by PDZ-GEF^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^VP16.PDZ-GEF

(Boettner and Van Aelst, 2007)

cno^mis1/cno² has ommatidium phenotype, non-enhanceable by faf^FO8/faf[+]

(Chen et al., 2000)

Suppressed by

Statement

Reference

cno^mis1/cno² has ommatidium phenotype, suppressible by fred[+]/fred^H24

(Fetting et al., 2009)

cno² has embryo | dorsal closure stage phenotype, suppressible by Scer\GAL4^ptc-559.1/bsk^UAS.cBa

(Boettner et al., 2003)

NOT suppressed by

Statement

Reference

cno² has embryo phenotype, non-suppressible by Scer\GAL4^VP16.PDZ-GEF/PDZ-GEF^UAS.cBa

(Boettner and Van Aelst, 2007)

cno² has embryonic/first instar larval cuticle | embryonic stage phenotype, non-suppressible by Scer\GAL4^VP16.PDZ-GEF/PDZ-GEF^UAS.cBa

(Boettner and Van Aelst, 2007)

cno² has ectoderm | embryonic stage phenotype, non-suppressible by Scer\GAL4^VP16.PDZ-GEF/PDZ-GEF^UAS.cBa

(Boettner and Van Aelst, 2007)

cno² has embryo phenotype, non-suppressible by PDZ-GEF^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^VP16.PDZ-GEF

(Boettner and Van Aelst, 2007)

cno² has embryonic/first instar larval cuticle | embryonic stage phenotype, non-suppressible by PDZ-GEF^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^VP16.PDZ-GEF

(Boettner and Van Aelst, 2007)

cno² has ectoderm | embryonic stage phenotype, non-suppressible by PDZ-GEF^{UAS.Tag:Myr(Src42A)}/Scer\GAL4^VP16.PDZ-GEF

(Boettner and Van Aelst, 2007)

cno² has embryo | dorsal closure stage phenotype, non-suppressible by Scer\GAL4^ptc-559.1/Rap1^{V12.UAS.Tag:MYC}

(Boettner et al., 2003)

Enhancer of

Statement

Reference

cno^mis1/cno² is an enhancer of embryo phenotype of PDZ-GEF^C1/PDZ-GEF^k13720

(Boettner and Van Aelst, 2007)

cno^mis1/cno² is an enhancer of embryo phenotype of PDZ-GEF^k13720/PDZ-GEF^C2

(Boettner and Van Aelst, 2007)

cno²/cno[+] is an enhancer of eye phenotype of mbt^P3

(Menzel et al., 2007)

cno² is an enhancer of ommatidium phenotype of S^48-5

(Gaengel and Mlodzik, 2003)

NOT Enhancer of

Statement

Reference

cno²/cno[+] is a non-enhancer of ommatidium phenotype of faf^FO8/faf^BX3

(Chen et al., 2000)

Suppressor of

Statement

Reference

cno² is a suppressor | partially of wing phenotype of PDZ-GEF^UAS.EGFP, Scer\GAL4^en.PU

(Boettner and Van Aelst, 2007)

cno² is a suppressor | partially of wing vein L2 phenotype of PDZ-GEF^UAS.EGFP, Scer\GAL4^en.PU

(Boettner and Van Aelst, 2007)

cno² is a suppressor | partially of wing vein L3 phenotype of PDZ-GEF^UAS.EGFP, Scer\GAL4^en.PU

(Boettner and Van Aelst, 2007)

Other

Statement

Reference

cno², wts^x1/wts[+] has larval RP2 motor neuron | embryonic stage phenotype

(Keder et al., 2015)

cno², ex¹/ex[+] has larval RP2 motor neuron | embryonic stage phenotype

(Keder et al., 2015)

Mer[+]/Mer⁴, cno² has larval RP2 motor neuron | embryonic stage phenotype

(Keder et al., 2015)

cno², mats^e03077/mats[+] has larval RP2 motor neuron | embryonic stage phenotype

(Keder et al., 2015)

cno², sav[+]/sav³ has larval RP2 motor neuron | embryonic stage phenotype

(Keder et al., 2015)

cno², yki[+]/yki^B5 has larval RP2 motor neuron | embryonic stage phenotype

(Keder et al., 2015)

cno², ft^G-rv/ft[+] has larval RP2 motor neuron | embryonic stage phenotype

(Keder et al., 2015)

cno^mis1/cno², zip^Ebr has wing phenotype

(Boettner and Van Aelst, 2007)

Additional Comments

Genetic Interactions

Statement

Reference

The proportion of hemisegments with abnormal number of neurons in the asymmetrically dividing RP2 neural lineage is significantly increased in embryos that are double heterozygous for cno² and any of the following: wts^x1, mats^e03077, sav³, yki^B5, ft^G-rv, ex¹ or Mer⁴. No such significant increase is observed in embryos double heterozygous for cno² and hpo^KC202.

(Keder et al., 2015)

ed^1X5 dominantly enhances the phenotype of mis-rotation of ommatidia that is seen in cno²/cno^mis1 animals.

fred^H24 dominantly suppresses the phenotype of mis-rotation of ommatidia that is seen in cno²/cno^mis1 animals.

(Fetting et al., 2009)

cno²/+ enhances the rough eye phenotype due to mbt^P3/Y.

(Menzel et al., 2007)

Homozygosity for R^CD5 enhances the cno²/cno^mis1 phenotype; only 4% of cno^mis1 R^CD5/cno² R^CD5 double mutant embryos develop into larvae or have a completely closed cuticle, 37% have large anterior holes in the cuticle and 59% are completely open dorsally. The dorsal closure defects seen in cno² embryos are not rescued by co-expression of expressing R^{V12.Scer\UAS.T:Hsap\MYC} under the control of Scer\GAL4^ptc-559.1. Expression of bsk^Scer\UAS.cBa under the control of Scer\GAL4^ptc-559.1 results in a significant but partial rescue of the dorsal closure defects seen in cno² embryos.

(Boettner et al., 2003)

S^48-5/cno² mutants show 40.56%+-7.99 misrotated ommatidia compared to 9.55%+-1.43 seen in S^48-5 mutants alone.

(Gaengel and Mlodzik, 2003)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Partially rescued by

cno² is partially rescued by Scer\GAL4^ptc-559.1/cno^{RA1mut+RA2mut.UAS}

(Boettner et al., 2003)

cno² is partially rescued by Scer\GAL4^ptc-559.1/cno^ΔN.UAS

(Boettner et al., 2003)

cno² is partially rescued by Scer\GAL4^ptc-559.1/cno^UAS.cBa

(Boettner et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

3107

ru¹ hry¹ Diap1^th-1 st¹ cno² cu¹ sr¹ e^s ca¹/TM3, Sb¹ Ser¹

Kyoto

106848

ru¹ hry¹ Diap1^th-1 st¹ cno² cu¹ sr¹ e^s ca¹/TM3, Sb¹ Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

canoe^9K

(Arora et al., 1995)

cno-2

(Gaengel and Mlodzik, 2003)

cno²

(Gurley et al., 2023, Dollar et al., 2016, Keder et al., 2015, Goossens et al., 2011, Slováková and Carmena, 2011, Fetting et al., 2009, Boettner and Van Aelst, 2007, Menzel et al., 2007)

cno^9K104

(Miyamoto et al., 1995)

cno^9K

(Tearle and Nusslein-Volhard, 1987)

dlhA¹

Name Synonyms

Secondary FlyBase IDs

FBal0002690

References (16)

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