Allele Dmel\ctK
| General Information | |||
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| Symbol | Dmel\ctK | Species | D. melanogaster |
| Name | of Krivshenko | FlyBase ID | FBal0002000 |
| Feature type | allele | Associated gene | Dmel\ct |
| Allele class | |||
| Mutagen | spontaneous | ||
Recent Updates
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| Description |
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| FB2013_03 | |||
| FB2013_02 | |||
| All updates | Click here to see a list of all updates to this record from FB2010_08 and on. | ||
Nature of the Allele
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| Allele class | |||
| Mutagen | |||
| Mutations Mapped to the Genome | |||
Type Location Additional Notes References | |||
| Associated Sequence Data | |||
| DDBJ
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EMBL / GenBank | DNA sequence Protein sequence Name | ||
| UniProtKB/Swiss-Prot | |||
| UniProtKB/TrEMBL | |||
| Progenitor genotype | |||
| Nature of the lesion | Statement Reference Insertion of gypsy element 6kb upstream of the 5'-most identified exon, in orientation opposite to that of cut. The gypsy element has a 129bp deletion that extends from the 1st to the 6th octamer repeat (proposed to interact with the su(Hw) product), spanning total of 5 repeats. Insertion of gypsy element at nucleotide position -48. DNA coordinates (FBrf0042060) (0 is gypsy site in ct6): +70.8 to +73.3 | ||
| Caused by insertion | |||
| Cytology | Polytene chromosomes normal. Polytene chromosomes normal. | ||
Phenotypic Data
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Phenotypic Class
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Phenotype Manifest In
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scutellum & macrochaeta scutum & macrochaeta | |||
Detailed Description
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Statement Reference There is a partial loss of the adult wing margin. ct[K] mutant flies show an intermediate "cut" wing phenotype, with partial loss of the wing margin. ctK mutant flies exhibit a scalloped wing phenotype with margin nicks. Hemizygotes have a decreased number of wing margin bristles and show some incision of wing blade tissue. Mutants have a wing nicking phenotype. Shows no interaction with pb mutations. Adult survivors exhibit wing margin defects. Homozygous embryos lack Malpighian tubules and the gut wall is 3--4 fold thicker than wild type at the junction of the posterior and anterior midgut. Flies have scalloped wing margins and fine scutellar bristles. Scalloped wings. Bristles are finer than wild-type. Completely complemented by Df(1)R19. Mutants have a number of cuts on the internal and external sides of the wing (resembling sd mutations). The macrochaetae of the mesonotum and especially the scutellum are of the Minute type. cut wings fine bristles Classified as a group-I lethal because homozygotes show reduced viability and only 10% of fewer heterozygotes with other lethal alleles survive; survivors have weakly cut wings, as well as fine bristles and enlarged and deformed humeral callus, not seen in other cut-wing mutants. | |||
External Data
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| Linkouts | |||
Interactions
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Phenotypic Class
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Enhanced by | |||
Statement Reference ctK has visible phenotype, enhanceable by Nipped-Ba.Sym.Scer\UAS/Nipped-BdsRNA.Sym.Scer\UAS/Scer\GAL4hs.PB | |||
NOT Enhanced by | |||
Statement Reference ctK has visible phenotype, non-enhanceable by CG4203dsRNA.Sym.Scer\UAS/CG4203a.Sym.Scer\UAS/Scer\GAL4hs.PB | |||
Suppressed by | |||
Statement Reference ctK has visible phenotype, suppressible | partially by Scer\GAL4bbg-C96/brmK804R.Scer\UAS.T:Ivir\HA1 | |||
NOT suppressed by | |||
Statement Reference ctK has visible phenotype, non-suppressible by CG4203dsRNA.Sym.Scer\UAS/CG4203a.Sym.Scer\UAS/Scer\GAL4hs.PB | |||
Enhancer of | |||
Statement Reference | |||
Other | |||
Statement Reference | |||
Phenotype Manifest In
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Enhanced by | |||
Statement Reference ctK has wing phenotype, enhanceable by Nipped-Ba.Sym.Scer\UAS/Nipped-BdsRNA.Sym.Scer\UAS/Scer\GAL4hs.PB | |||
NOT Enhanced by | |||
Statement Reference ctK has wing phenotype, non-enhanceable by CG4203dsRNA.Sym.Scer\UAS/CG4203a.Sym.Scer\UAS/Scer\GAL4hs.PB | |||
Suppressed by | |||
Statement Reference ctK has triple row phenotype, suppressible | partially by Scer\GAL4bbg-C96/brmK804R.Scer\UAS.T:Ivir\HA1 | |||
NOT suppressed by | |||
Statement Reference ctK has wing phenotype, non-suppressible by CG4203dsRNA.Sym.Scer\UAS/CG4203a.Sym.Scer\UAS/Scer\GAL4hs.PB | |||
Additional Comments
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Genetic Interactions
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Statement Reference The "cut" wing phenotype observed in ct[K] mutant animals is almost entirely rescued by one copy of mod(mdg4)[ul], and completely rescued in animals carrying two copies of mod(mdg4)[ul].
The "cut" wing phenotype observed in ct[K] mutant animals is suppressed in a mod(mdg4)[T6] mutant background. ctK SMC1exc46 heterozygous double mutants exhibit a reduced number of wing margin nicks compared to ctK mutants.
Sse13m heterozygosity does not affect the wing margin nick phenotype of ctK mutants.
sanKG04816 heterozygosity does not affect the wing margin nick phenotype of ctK mutants.
eco1 heterozygosity does not affect the wing margin nick phenotype of ctK mutants.
ctK mutants heterozygous for pds5e3 exhibit a slightly increased number of wing margin nicks compared to ctK heterozygotes, although this is not significantly different from that seen in ctK single mutants.
ctK mutants heterozygous for pds5e6 exhibit a significantly decreased level of wing margin nicking compared to ctK single mutants. Expression of wgScer\UAS.cLa under the control of Scer\GAL4C96 suppresses the loss of wing blade tissue, including the slender recurved chemosensory wing margin bristles, seen in ctK flies, but does not rescue the loss of the stout mechanosensory wing margin bristles. The wing nicking phenotype seen in ct[K] mutant males is not affected by expression of CG4203[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[hs.PB].
The severity of the wing nicking phenotype seen in ct[K] mutant males is increased by expression of Nipped-B[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[hs.PB].
The severity of the wing nicking phenotype seen in ct[K] mutant males is decreased by expression of SA[dsRNA.Sym.Scer\UAS] under the control of Scer\GAL4[hs.PB]. ctK phenotype is completely suppressed by mod(mdg4)ul. | |||
Xenogenetic Interactions
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Statement Reference | |||
Complementation & Rescue Data
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| Partially rescued by | |||
| Comments | Expression of mod(mdg4)[Scer\UAS.cGa] or mod(mdg4)[ΔQ.Scer\UAS] under the control of Scer\GAL4[Act5C] in a ct[K],mod(mdg4)[ul] mutant background suppresses the effect that mod(mdg4)[ul] has on ct[K] wings, and the wings that form lack regions of the wing margin and are similar to ct[K] wings. | ||
Stocks
( 2 ) | |||
| Bloomington | 171 | ||
| Kyoto | 105768 | ||
Notes on Origin
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| Discoverer | Krivshenko. | ||
Comments
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Weak lethal class I ct allele. A cut lethal I phenotypic class allele. | |||
External Crossreferences & Linkouts
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| Other Crossreferences | |||
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Synonyms & Secondary IDs
( 3 ) | |||
| Reported As | |||
| Symbol Synonym | ctk | ||
| Name Synonym | of Krivshenko | ||
| Secondary FlyBase IDs | |||
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References
( 23 ) | |||
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Recent research papers (0)
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| All research papers listed in FlyBase were published before 2011 | |||
Recent Updates
External Crossreferences & Linkouts