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General Information
Symbol
Dmel\cuffWM25
Species
D. melanogaster
Name
FlyBase ID
FBal0002144
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
cuffWM
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

T11886112A

Reported nucleotide change:

T?A

Amino acid change:

M1K | cuff-PA

Reported amino acid change:

M1K

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: M1K.

Nucleotide substitution: T2A.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Eggs laid by cuffWM25, cuffWM25/cuffKG05951 and cuffWM25/cuffQQ37 females show a variable ventralised phenotype.

The oocyte nucleus has defective morphology in 10-20% of stage 9 egg chambers in cuffWM25/cuffKG05951 females, often the DNA seems to localise to the periphery of the nucleus.

In newly eclosed cuffWM25/cuffKG05951 females, highly branched fusomes are observed in regions 1 and 2 of the germaria (as occurs in wild-type females). However, as the mutant females age, there is a sharp increase in the number of germaria without cysts that contain highly branched fusomes; 1 week after eclosion, 54% of the germaria do not have highly branched fusomes and 2 weeks after eclosion, 83% of the germaria do not have highly branched fusomes in the mutant females. The mutant cysts in older female appear to arrest in early stages in the germaria containing spectrosome-like structures. In addition, the overall morphology of germline cysts in region 2b and/or region 3 of the germaria is also disrupted in cuffWM25/cuffKG05951 females; 1 week after eclosion, 72% of the germaria do not have cysts of normal morphology and 2 weeks after eclosion, 84% of the germaria do not have cysts of normal morphology.

Absence of egg chambers or degeneration of early egg chambers.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The segregation distortion seen in males carrying either the SD-5star or SD-LosArenos Dp(2;2)RanGAPSD chromosome variant (in a sensitive Rsp background) is enhanced (the k[[c]] value, or proportion of Dp(2;2)RanGAPSD-bearing progeny as the fraction of total progeny (corrected for viability), is significantly increased) if the males are also carrying a single copy of cuffWM25.

The ventralised phenotype seen in eggs derived from cuffWM25 and cuffWM25/cuffQQ37 females is partially suppressed by lokunspecified. The defects in germline cyst development seen in cuffWM25 females are partially suppressed by lokunspecified; many highly branched fusomes are seen in the double mutant germaria.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (9)