Allele Dmel\CycA³

General Information
Symbol	Dmel\CycA³	Species	D. melanogaster
Name		FlyBase ID	FBal0002201
Feature type	allele	Associated gene	Dmel\CycA
Also Known As	l(3)183, cycA¹⁸³

Allele class	amorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	amorphic allele - genetic evidence (Greaves et al., 1999)
Mutagen	ethyl methanesulfonate (Lehner and O'Farrell, 1989)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference A large deletion that removes the complete CycA locus and extends into flanking genes. (Lehner, 2013.1.1) Deletion of CycA coding sequence. (Lehner et al., 1991) Small deletion that removes cyclin sequences. (Lehner and O'Farrell, 1989) molecular deletion
Caused by aberration	Df(3L)CycA³ (Lehner, 2013.1.1)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| recessive (Lehner and O'Farrell, 1989) mitotic cell cycle defective (Berger et al., 2005, Luer et al., 1997, Lehner et al., 1991) mitotic cell cycle defective \| embryonic stage (with CycA⁵) (Reber et al., 2006) neuroanatomy defective (Berger et al., 2005)
Phenotype Manifest In
	dorsal neurohemal organ (Luer et al., 1997) embryonic epidermis (Lehner et al., 1991) embryonic epidermis (with CycA⁵) (Reber et al., 2006) EW neuron (Novotny et al., 2002) GW neuron (Novotny et al., 2002) mitotic cell cycle (Knoblich and Lehner, 1993) neuroblast NB6-4 of abdomen (Akiyama-Oda et al., 2000) neuroblast NB6-4 of thorax (Akiyama-Oda et al., 2000, Berger et al., 2005) neuroblast NB7-3 (Novotny et al., 2002)
Detailed Description
	Statement Reference CycA⁵/CycA³ embryos show a failure of epidermal cells to undergo mitosis 16. (Reber et al., 2006) In CycA³ mutant embryos, division of the NB6-4t lineage is arrested afer the first or second division. However, the first division of this lineage produces one glial cell and one neuronal cell as in wild-type. (Berger et al., 2005) Only one, two or three cells are found in the position of the NB7-3 clusters (8%, 75% and 17% respectively) compared to the four seen in wild-type. (Novotny et al., 2002) In mutant embryos, two to four progeny cells of NB6-4T appear in each hemisegment, as opposed to the 5 to 6 cells seen in wild-type. In the abdominal segment of this mutant, more than half of NB6-4A divided once into 2 cells. The first cell division. the onset of glial differentiation and cell fate bifurcation occur normally in these embryos. (Akiyama-Oda et al., 2000) Mitosis is arrested during the 16th cell cycle. Cells are stillable to differentiate and dorsal medial cells carrying bilateral cytoplasmic extensions are clearly identifiable in late embryos. Scer\GAL4^sim.PS-mediated expression of spi^s.Scer\UAS increases the number of dorsal medial cells, the implantation of one or two midline cells from these donors into CycA mutant embryos fails to induce additional dorsal medial cells. (Luer et al., 1997) In embryos double mutant for CycA³, no mitotic figures are observed at mitosis 15. BrdU incorporations reveal that the block is in the preceding G₂. (Knoblich and Lehner, 1993) CycA^hari/CycA³ heterozygotes exhibit a 'hari' phenotype. (Ueda et al., 1992) Entry into mitosis 16 does not occur in the mutant cells of the epidermis. Cells do not exhibit complete cell cycle arrest. (Lehner et al., 1991)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference CycA³ is an enhancer of visible phenotype of Scer\GAL4^en-e16E, arm^Scer\UAS.cWa (Greaves et al., 1999)
NOT Enhancer of
Statement Reference CycA³ is a non-enhancer of visible phenotype of Scer\GAL4^en-e16E, aos::shg^{i.Scer\UAS.T:Hsap\MYC} (Greaves et al., 1999)
Suppressor of
Statement Reference CycA³/CycA[+] is a suppressor \| partially of lethal \| partially phenotype of dally^ΔP-305 (Nakato et al., 2002) CycA³/CycA[+] is a suppressor \| partially of visible phenotype of dally⁰⁶⁴⁶⁴ (Nakato et al., 2002) CycA³/CycA[+] is a suppressor of lethal \| partially phenotype of dally⁰⁶⁴⁶⁴ (Nakato et al., 2002) CycA³ is a suppressor of visible phenotype of rux³ (Thomas et al., 1994)
NOT Suppressor of
Statement Reference CycA³/CycA[+] is a non-suppressor of increased cell death phenotype of shtd¹ (Tanaka-Matakatsu et al., 2007) CycA³ is a non-suppressor of visible phenotype of Scer\GAL4^en-e16E, aos::shg^{i.Scer\UAS.T:Hsap\MYC} (Greaves et al., 1999)
Phenotype Manifest In
Suppressed by
Statement Reference CycA³ has mitotic cell cycle phenotype, suppressible by CycB^hs.PK (Knoblich and Lehner, 1993)
Enhancer of
Statement Reference CycA³ is an enhancer of wing phenotype of Scer\GAL4^en-e16E, arm^Scer\UAS.cWa (Greaves et al., 1999)
NOT Enhancer of
Statement Reference CycA³ is a non-enhancer of wing phenotype of Scer\GAL4^en-e16E, aos::shg^{i.Scer\UAS.T:Hsap\MYC} (Greaves et al., 1999)
Suppressor of
Statement Reference CycA³/CycA[+] is a suppressor \| partially of antenna phenotype of dally⁰⁶⁴⁶⁴ (Nakato et al., 2002) CycA³/CycA[+] is a suppressor \| partially of lamina anlage & mitotic cell cycle phenotype of dally⁰⁶⁴⁶⁴ (Nakato et al., 2002) CycA³/CycA[+] is a suppressor \| partially of wing vein L5 phenotype of dally⁰⁶⁴⁶⁴ (Nakato et al., 2002) CycA³/CycA[+] is a suppressor of ommatidium phenotype of dally⁰⁶⁴⁶⁴ (Nakato et al., 2002) CycA³ is a suppressor of eye phenotype of rux³ (Thomas et al., 1994)
NOT Suppressor of
Statement Reference CycA³/CycA[+] is a non-suppressor of eye phenotype of shtd¹ (Tanaka-Matakatsu et al., 2007) CycA³/CycA[+] is a non-suppressor of ommatidium phenotype of shtd¹ (Tanaka-Matakatsu et al., 2007) CycA³ is a non-suppressor of wing phenotype of Scer\GAL4^en-e16E, aos::shg^{i.Scer\UAS.T:Hsap\MYC} (Greaves et al., 1999)
Additional Comments
Genetic Interactions
Statement Reference The lethality seen in dally⁰⁶⁴⁶⁴ homozygotes is completely suppressed if they are also heterozygous for CycA³. The penetrance of the eye, antenna and wing defects seen in dally⁰⁶⁴⁶⁴ homozygotes is reduced if they are also heterozygous for CycA³. The severity of the dally⁰⁶⁴⁶⁴ eye defects are also reduced in the double mutants. The lethality seen in dally^ΔP-305 homozygotes is reduced if they are also heterozygous for CycA³. The ability of dally⁰⁶⁴⁶⁴ lamina precursor neurons to enter a second division cycle is largely restored if they are also carrying CycA³. (Nakato et al., 2002) No effect on the faf eye phenotype. (Huang and Fischer-Vize, 1996) Dominantly restores 81% of ommatidia of rux³ to wild type. (Thomas et al., 1994) The mitotic cycle phenotype can be rescued by heat shock induced expression of CycB^hs.PK. (Knoblich and Lehner, 1993)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	CycA³ is rescued by CycA^hs.PK (Knoblich and Lehner, 1993)
Comments	This phenotype can be rescued by heat shock induced expression of CycA^hs.PK. (Knoblich and Lehner, 1993)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	cycA³ (Nakato et al., 2002, Luer et al., 1997) CycA³ (Lehner, 2013.1.1) cycA¹⁸³ (Akiyama-Oda et al., 2000, Greaves et al., 1999) CycA¹⁸³ (Reber et al., 2006) cycA^l(3)183 (Thomas et al., 1994) l(3)183 (Ohlmeyer and Schupbach, 2003, Huang and Fischer-Vize, 1996, Ueda et al., 1992, Lehner et al., 1991, Lehner and O'Farrell, 1989, Tanaka-Matakatsu et al., 2007, Lehner, 2013.1.1)
Name Synonym
Secondary FlyBase IDs

References ( 18 )
Research paper	Tanaka-Matakatsu et al., 2007, Dev. Biol. 309(2): 222--235 Mutation of the Apcl homologue shattered disrupts normal eye development by disrupting G1 cell cycle arrest and progression through mitosis. [FBrf0201230] Reber et al., 2006, Development 133(16): 3201--3211 Terminal mitoses require negative regulation of Fzr/Cdh1 by Cyclin A, preventing premature degradation of mitotic cyclins and String/Cdc25. [FBrf0195204] Berger et al., 2005, Nat. Cell Biol. 7(1): 56--62 A critical role for Cyclin E in cell fate determination in the central nervous system of Drosophila melanogaster. [FBrf0184186] Ohlmeyer and Schupbach, 2003, Development 130(25): 6339--6349 Encore facilitates SCF-Ubiquitin-proteasome-dependent proteolysis during Drosophila oogenesis. [FBrf0167518] Nakato et al., 2002, J. Cell Sci. 115(1): 123--130 dally, a Drosophila member of the glypican family of integral membrane proteoglycans, affects cell cycle progression and morphogenesis via a Cyclin A-mediated process. [FBrf0144952] Novotny et al., 2002, Development 129(4): 1027--1036 Hunchback is required for the specification of the early sublineage of neuroblast 7-3 in the Drosophila central nervous system. [FBrf0144815] Akiyama-Oda et al., 2000, Dev. Biol. 222(2): 429--439 Distinct mechanisms triggering glial differentiation in Drosophila thoracic and abdominal neuroblasts 6-4. [FBrf0128371] Greaves et al., 1999, Genetics 153(4): 1753--1766 A screen for identifying genes interacting with armadillo, the Drosophila homolog of beta-catenin. [FBrf0123026] Luer et al., 1997, Development 124(14): 2681--2690 Induction of identified mesodermal cells by CNS midline progenitors in Drosophila. [FBrf0095612] Huang and Fischer-Vize, 1996, Development 122(10): 3207--3216 Undifferentiated cells in the developing Drosophila eye influence facet assembly and require the Fat facets ubiquitin-specific protease. [FBrf0090618] Sauer et al., 1995, Genes Dev. 9(11): 1327--1339 Distinct modes of cyclin E/cdc2c kinase regulation and S-phase control in mitotic and endoreduplication cycles of Drosophila embryogenesis. [FBrf0082560] Thomas et al., 1994, Cell 77(7): 1003--1014 Cell cycle progression in the developing Drosophila eye: roughex encodes a novel protein required for the establishment of G1. [FBrf0074608] Knoblich and Lehner, 1993, EMBO J. 12(1): 65--74 Synergistic action of Drosophila cyclins A and B during the G₂-M transition. [FBrf0058164] Ueda et al., 1992, EMBO J. 11: 2935--2939 Sensory mother cell division is specifically affected in a Cyclin-A mutant of Drosophila melanogaster. [FBrf0055951] Lehner et al., 1991, Cold Spring Harbor Symp. Quant. Biol. 56: 465--475 Exploring the role of Drosophila cyclin A in the regulation of S phase. [FBrf0053446] Lehner and O'Farrell, 1989, Cell 56: 957--968 Expression and function of Drosophila cyclin A during embryonic cell cycle progression. [FBrf0049390] Hoogwerf et al., 1988, Genetics 118: 665--670 A genetic analysis of the rose-gespleten region (68C8-69B5) of Drosophila melanogaster. [FBrf0048238]
Personal communication to FlyBase	Lehner, 2013.1.1, Df(3L)CycA[3]. Df(3L)CycA[3]. [FBrf0220457]

Allele Dmel\CycA3

Allele Dmel\CycA³