Allele Dmel\Nrt^M2

General Information
Symbol	Dmel\Nrt^M2	Species	D. melanogaster
Name		FlyBase ID	FBal0002234
Feature type	allele	Associated gene	Dmel\Nrt
Also Known As	dab^M2

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Gertler et al., 1989, Liebl et al., 2003)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3L:16,768,310..16,768,310 reported_na_change=T?A reported_pr_change=L464@ evidence=experimental pr_change=L464\|Nrt-PA,L464\|Nrt-PB na_change=T16768310A (Liebl et al., 2003)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: L464@. Nucleotide substitution: T?A. (Liebl et al., 2003)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage \| recessive (Bennett and Hoffmann, 1992)
Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference Abl¹/Df(3L)st-j7, Nrt^M2 has lethal phenotype, suppressible \| partially by Scer\GAL4³¹/Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS} (Fogerty et al., 1999) Abl¹/Df(3L)st-j7, Nrt^M2 has lethal phenotype, suppressible \| partially by Scer\GAL4³¹/Abl::Hsap\ABL1::Hsap\BCR^{P210.Scer\UAS} (Fogerty et al., 1999) Abl¹/Df(3L)st-j7, Nrt^M2 has lethal phenotype, suppressible \| partially by Scer\GAL4³¹/Abl¹/Abl^{K417N.Scer\UAS}/Df(3L)st-j7 (Fogerty et al., 1999) Abl¹/Df(3L)st-j7, Nrt^M2 has lethal phenotype, suppressible \| partially by Scer\GAL4³¹/Abl^Scer\UAS.cFa (Fogerty et al., 1999)
Enhancer of
Statement Reference Nrt^M2 is an enhancer of lethal phenotype of Abl¹ (Gertler et al., 1989) Nrt^M2 is an enhancer of lethal phenotype of Abl² (Gertler et al., 1989)
Other
Statement Reference Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS}, Abl¹/Df(3L)st-j7, Nrt^M2, Scer\GAL4³¹ has visible phenotype (Fogerty et al., 1999) Abl::Hsap\ABL1::Hsap\BCR^{P210.Scer\UAS}, Abl¹/Df(3L)st-j7, Nrt^M2, Scer\GAL4³¹ has visible phenotype (Fogerty et al., 1999) Abl¹/Df(3L)st-j7, Nrt^M2 has lethal phenotype (Fogerty et al., 1999) Abl¹/Df(3L)st-j7, Nrt^M54/Nrt^M2 has neuroanatomy defective phenotype (Liebl et al., 2003, Liebl et al., 2003)
Phenotype Manifest In
Other
Statement Reference Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS}, Abl¹/Df(3L)st-j7, Nrt^M2, Scer\GAL4³¹ has eye phenotype (Fogerty et al., 1999) Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS}, Abl¹/Df(3L)st-j7, Nrt^M2, Scer\GAL4³¹ has ommatidium phenotype (Fogerty et al., 1999) Abl::Hsap\ABL1::Hsap\BCR^{P210.Scer\UAS}, Abl¹/Df(3L)st-j7, Nrt^M2, Scer\GAL4³¹ has eye phenotype (Fogerty et al., 1999) Abl::Hsap\ABL1::Hsap\BCR^{P210.Scer\UAS}, Abl¹/Df(3L)st-j7, Nrt^M2, Scer\GAL4³¹ has ommatidium phenotype (Fogerty et al., 1999) Abl¹/Df(3L)st-j7, Nrt^M2 has axon phenotype (Gertler et al., 1993, Gertler et al., 1993) Abl¹/Df(3L)st-j7, Nrt^M2 has commissure phenotype (Gertler et al., 1993, Gertler et al., 1993) Abl¹/Df(3L)st-j7, Nrt^M54/Nrt^M2 has ventral nerve cord commissure phenotype (Liebl et al., 2003, Liebl et al., 2003)
Additional Comments
Genetic Interactions
Statement Reference 63% of segments have commissure defects in the central nervous system of Abl¹ Nrt^M54/Nrt^M2 Df(3L)st-j7 embryos. (Liebl et al., 2003) The lethality of Abl¹/Nrt^M2 Df(3L)st-j7 is partially rescued by Abl^Scer\UAS.cFa or Abl^{K417N.Scer\UAS}, expressed under the control of Scer\GAL4³¹. (Fogerty et al., 1999) fax^M7 Abl¹/In(3L)std11 and fax^M12 Abl¹/In(3L)std11 individuals are lethal due to disruptions in the CNS longitudinal and commissural axons. The presence of Nrt^M2 does not affect the lethality. Dosage sensitive interactions exist between Nrt^M2, fax^M7 and fax^M12. Abl^+mTnabl is unable to rescue the lethality of fax^- Nrt^- individuals. (Hill et al., 1995) Abl¹/Df(3L)st-j7 Nrt^M2 double mutant causes absence of most intersegmental longitudinal axon bundles and most commissural axon bundles. The lethality of Nrt^M2 Df(3L)st-j7/Abl¹ animals is rescued by four copies of P{Dab.G} to almost full viability. (Gertler et al., 1993) Nrt^M2 Df(3L)st-j7/In(3L)std11 double mutant embryos show muscle absences and detachments. (Bennett and Hoffmann, 1992) Enhances the Abl¹ and Abl² mutant phenotype from pupal lethal to embryonic or larval lethality by an haploinsufficiency dependent on Abl (HDA). (Gertler et al., 1989)
Xenogenetic Interactions
Statement Reference The lethality of Abl¹/Nrt^M2 Df(3L)st-j7 is partially rescued by Abl::Hsap\ABL1::Hsap\BCR^{P210.Scer\UAS} or Abl::Hsap\ABL1::Hsap\BCR^{P185.Scer\UAS} expressed under the control of Scer\GAL4³¹. (Fogerty et al., 1999)
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer
	Induced on: Df(3L)st-j7. The Nrt^M2 mutant allele was originally thought to be a mutation in the Dab gene (see FBrf0058531 and FBrf0084025), but sequencing of the chromosome indicates that it is a lesion in the Nrt gene. (Liebl et al., 2003) Induced on: Df(3L)st-j7. (Gertler et al., 1989)
Comments
	Haploinsufficiency dependent upon an Abl mutant background (HDA). (Hill et al., 1995) Complements: l(3)73Bc^unspecified. (Gertler et al., 1989)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 7 )
Reported As
Symbol Synonym	Dab¹ dab^M2 (Liebl et al., 2003, Gertler et al., 1989) dab^m2 (Fogerty et al., 1999, Hill et al., 1995, Bennett and Hoffmann, 1992, Belote et al., 1990) Dab^m2 (Gertler et al., 1993) M2 (Gertler et al., 1989, ) nrt^M2 (Liebl et al., 2003) Nrt^M2
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Liebl et al., 2003, Development 130(14): 3217--3226 Interactions between the secreted protein Amalgam, its transmembrane receptor Neurotactin and the Abelson tyrosine kinase affect axon pathfinding. [FBrf0162067] Fogerty et al., 1999, Oncogene 18(1): 219--232 Dominant effects of the bcr-abl oncogene on Drosophila morphogenesis. [FBrf0106495] Hill et al., 1995, Genetics 141(2): 595--606 Genetic interactions between the Drosophila Abelson, Abl, tyrosine kinase and failed axon connections (Fax), a novel protein in axon bundles. [FBrf0084025] Gertler et al., 1993, Genes Dev. 7(3): 441--453 Dosage-sensitive modifiers of Drosophila Abl tyrosine kinase function: prospero, a regulator of axonal outgrowth, and disabled, a novel tyrosine kinase substrate. [FBrf0058531] Bennett and Hoffmann, 1992, Development 116(4): 953--966 Increased levels of the Drosophila Abelson tyrosine kinase in nerves and muscles: subcellular localization and mutant phenotypes imply a role in cell-cell interactions. [FBrf0055913] Belote et al., 1990, Genetics 125: 783--793 Cytogenetic analysis of chromosome region 73AD of Drosophila melanogaster. [FBrf0051976] Gertler et al., 1989, Cell 58: 103--113 Drosophila abl tyrosine kinase in embryonic CNS axons: a role in axonogenesis is revealed through dosage-sensitive interactions with disabled. [FBrf0049327]

Allele Dmel\NrtM2

Allele Dmel\Nrt^M2