FlyBase Allele Report: Dmel\DNaseII[lo]

General Information

Symbol

Dmel\DNaseII^lo

Species

D. melanogaster

Name

FlyBase ID

FBal0002709

Feature type

allele

Associated gene

Dmel\DNaseII

Associated Insertion(s)

Carried in Construct

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Progenitor genotype

Cytology

Description

Amino acid replacement: S?N.

(Evans et al., 2002)

Nucleotide substitution: G668A. Amino acid replacement: S223N.

(Evans et al., 2002)

Nucleotide substitution: G?A. Amino acid replacement: S223N.

(Mukae et al., 2002)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3R:18,020,639..18,020,639 [+]

Nucleotide change:

G18020639A

Reported nucleotide change:

G668A

Amino acid change:

S223N | DNaseII-PA

Reported amino acid change:

S223N

(Evans et al., 2002)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

Detailed Description

Statement

Reference

DNaseII^lo homozygotes do not exhibit a significant increase in the proportion of hyperplastic testes, as compared to wild-type controls.

(Napoletano et al., 2017)

DNaseII^lo mutant males exhibit ~40% less spermatogonial cyst death compared to controls.

(Yacobi-Sharon et al., 2013)

Homozygous DNaseII^lo mutants develop normally to the adult stage.

(Liu et al., 2012)

DNaseII^lo homozygous egg chambers from animals subjected to starvation conditions to induce mid-oogenesis programmed cell death initiate degeneration normally. However, late stages egg chambers are opaque and show dispersed fragments of nurse cell DNA. DNaseII^lo germ-line clone derived egg chambers from animals subjected to starvation conditions to induce mid-oogenesis programmed cell death display a milder phenotype than homozygotes.

49% of DNaseII^lo stage 14 egg chambers show persisting nurse cell nuclei, compared to 7% in controls.

75% of DNaseII^lo/Df(3R)sr16 stage 14 egg chambers show persisting nurse cell nuclei. The persisting DNA is often smeared, unlike the discrete nuclei observed in wild-type mutants.

75% of DNaseII^lo germ line clone stage 14 egg chambers show persistant nurse cell nuclei.

(Bass et al., 2009)

Mutant embryos show an increase in the number of apoptotic cells compared to controls.

(Kupsco et al., 2006)

DNaseII^lo mutant flies are fertile, but their egg production rate is significantly reduced. Apoptotic DNA fragmentation is enhanced in DNaseII^lo mutant embryos and adult ovaries, compared to wild-type. The ovaries of DNaseII^lo mutant flies accumulate large amounts of acridine positive material in vesicles. These vesicles can be stained with Feulgen, suggesting accumulation of undigested DNA. A similar phenotype is seen in DNaseII^lo/Df(3R)P14 flies.

(Mukae et al., 2002)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

DNaseII^lo has abnormal cell number | maternal effect | embryonic stage phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Orc2^GL01560

(Tarayrah-Ibraheim et al., 2021)

DNaseII^lo has abnormal cell number | maternal effect | embryonic stage phenotype, suppressible by AIF^UAS.Tag:MYC/Scer\GAL4^{VP16.nanos.UTR}

(Tarayrah-Ibraheim et al., 2021)

DNaseII^lo has viable phenotype, suppressible by eya¹

(Liu et al., 2012)

DNaseII^lo has viable phenotype, suppressible by eya²

(Liu et al., 2012)

Other

Statement

Reference

DNaseII^lo, wun2^N14 has decreased cell number | maternal effect | embryonic stage phenotype

(Tarayrah-Ibraheim et al., 2021)

Phenotype Manifest In

Suppressed by

Statement

Reference

DNaseII^lo has primordial germ cell | maternal effect | embryonic stage phenotype, suppressible by Scer\GAL4^{VP16.nanos.UTR}/Orc2^GL01560

(Tarayrah-Ibraheim et al., 2021)

DNaseII^lo has primordial germ cell | maternal effect | embryonic stage phenotype, suppressible by AIF^UAS.Tag:MYC/Scer\GAL4^{VP16.nanos.UTR}

(Tarayrah-Ibraheim et al., 2021)

Other

Statement

Reference

DNaseII^lo, wun2^N14 has primordial germ cell | maternal effect | embryonic stage | decreased number phenotype

(Tarayrah-Ibraheim et al., 2021)

Additional Comments

Genetic Interactions

Statement

Reference

DNaseII^lo/+, p53^E8/+ adults do not exhibit a significant increase in the proportion of hyperplastic testes, as compared to p53^E8/+ adults, or wild-type controls.

(Napoletano et al., 2017)

Flies carrying homozygous eya¹ and DNaseII^lo mutations die at the pupal stage.

Flies carrying homozygous eya² and DNaseII^lo mutations die at the pupal stage.

(Liu et al., 2012)

68% of Drep-1^P; DNaseII^lo double mutant stage 14 egg chambers show persisting nurse cell nuclei, enhanced compared to each single mutant phenotype. The persisting DNA is smeared as observed in DNaseII^lo mutants, unlike the discrete nuclei observed in wild-type and Drep-1^P single mutants. 3% of egg chambers display a dumpless phenotype where nurse cell cytoplasm has not been transferred to the oocyte.

(Bass et al., 2009)

Apoptotic DNA fragmentation is not detectable in Rep1^P, DNaseII^lo double mutant embryos or adult ovaries. The ovaries of double mutant Rep1^P, DNaseII^lo flies accumulate large amounts of acridine positive material.

(Mukae et al., 2002)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

DNaseII^lo is rescued by Scer\GAL4^{VP16.nanos.UTR}/DNaseII^UAS.cTa

(Tarayrah-Ibraheim et al., 2021)

DNaseII^lo is rescued by DNaseII^UAS.cEa/Scer\GAL4^Act5C.PI

(Evans et al., 2002)

Comments

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