Immunolocalization of dnc protein to thin sections of adult brain shows that it is largely concentrated in the mushroom bodies. Within mushroom bodies, the γ lobes stain more intensely than the α or β lobes, peduncle staining intensity varies with position and calyx expression is uniform. An intermediate level of staining is seen in most neuropil structures including the inner optic lobe neuropil, protocerebrum, suboesophageal ganglion, antennal lobes, and central complex. A conspicuous lack of staining is found in the photoreceptor cell layer, lamina, antennal nerve, and median bundle. The pattern of dnc2 protein was indistinguishable in heads from the wild type pattern.
The egg laying and hatching defects of Gprk206936 homozygous females are partially suppressed by a single copy of dnc2. The defect in tethering of the nurse cell nuclei seen in Gprk206936 homozygous egg chambers is partially suppressed by a single copy of dnc2. dnc2/dnc2 females carrying Gprk206936/+ lay 1.3 eggs/female/hour (wild-type value is 2.9 eggs/female/hour) of which 32.3% hatch. dnc2/dncM14 females carrying Gprk206936/+ lay 1.5 eggs/female/hour of which 31.6% hatch. The egg chamber degeneration of dnc2 homozygotes and dnc2/dncM14 females is partially suppressed by Gprk206936/+.