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General Information
Symbol
Dmel\dncM14
Species
D. melanogaster
Name
FlyBase ID
FBal0002717
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

dnc1/dncM14 adults show a significant reduction in performance index in an aversive olfactory learning assay compared to control flies.

In contrast to wild type, type II neuromuscular junctions do not show an increase in the number of natural synaptopods in response to octopamine in homozygous third instar larvae.

dncM14 homozygous and dncM14/dncML heterozygous mutants exhibit an increase in the number of naturally occuring synaptopods at the type II endings. This increase is eliminated in a Dp(1;2)51b background.

Application of 10¤ůM octopamine fails to increase the number of synaptopods in dncM14 homozygous neuromuscular junctions.

dncM14 mutants display decreased locomotor speed and no response to starvation.

dnc1/dncM14 mutant flies show decreased avoidance of high temperatures.

Nurse cell dumping is incomplete in egg chambers from homozygous and dnc2/dncM14 females. Egg chambers have severely truncated dorsal appendages or the dorsal appendages fail to form altogether. Egg chamber degeneration is detected in stage 6 egg chambers and by stages 10 and 11, 88% of egg chambers are degenerating. Homozygous females lay only an occasional egg.

Nurse cell nuclei are enlarged compared to wild type in dncM14/Df(1)N-64i16 and dnc225/dncM14 females. Rare egg chambers containing 32 cells are seen. Females show egg retention.

Synaptic terminals of axons 1 and 2 (from neurons RP3 and 6/7b respectively) at the neuromuscular junction of muscles 6 and 7 of third instar dncM14/Y larvae have less dense presynaptic and postsynaptic membranes than control synaptic terminals. A similar number of synapses per unit length of terminal is seen compared to controls. Axon 2 terminals show an increase in synapse size. A much greater variability in synapse area is seen compared to controls. The ratio of docked to undocked vesicles is higher than in wild type in axon 1 synapses.

Neurons with a slowly inactivating outward current and down-modulated by cAMP are absent in homozygous larval brains in contrast to wild-type. Neurons with an outward current dominated by a rapidly inactivating outward current and not modulated by cAMP are more abundant in homozygous larval brains compared to wild-type.

The RP3 and 6/7b neurons which innervate longitudinal muscles 6 and 7 have overgrown terminals in dncM14 mutant larvae and establish a larger terminal surface over the muscles than in wild-type larvae.

Virgin females injected with sex peptide (SP) show no decline in receptivity towards courting males, no inhibition of remating associated with the transfer of any non-sperm components at mating (the 'copulation effect') but did show a loss of receptivity due to the receipt of sperm (the 'sperm effect'). There is no significant difference in lifespan between females that have mated once and virgin females. Twice mated females have a significantly shorter lifespan.

Increased bouton number and branching (due to down-regulation of synaptic Fas2 expression) and an increase in quantal content. Expression of CrebB-17Ab.hs does not alter the increase in synaptic structure but does reduce the quantal content to wild type levels. Another characteristic of the dnc phenotype is an alteration of synaptic modulation, wild type facilitation is replaced by depression at stimulation frequencies above 10Hz. Expression of CrebB-17Ab.hs significantly suppresses this aspect of the phenotype.

Mutant increases cAMP synthesis and moderately increases the rate of habituation (using the jump-and-flight escape response). Habituation is extremely rapid in dnc rut double mutants.

Growth cone exploratory movement is nearly arrested. Phenotype can be mimicked by normal neurons when perfused with db-cAMP (dibutyryl cAMP) or forskolin. Motility is also restored by counterbalancing the effects in rut dnc double mutants.

Number of boutons is increased compared to wild type due to reduction in Fas2 expression, but the mean quantal content or the quantal size is not altered reflecting no change in synaptic strength. Bouton number does not increase when Fas2Scer\UAS.cLa is expressed from Scer\GAL4E62-2 in dncM14 larvae.

Heterozygotes with dnc64j15 and dnc69h9 display reduced female fertility, numerous eggs laid generating some progeny. Heterozygotes with dnc81l6 are female sterile, no eggs are laid.

The voltage-activated transient K+ current (IA) in the larval muscle fibres of dncM14 animals is increased compared to wild-type. The amplitude of the transient and delayed plateau outward K+ currents (IT and IS respectively) in the larval muscle fibres of dncM14 animals are increased compared to wild-type. The amplitude of these currents is drastically reduced, to almost normal levels, if the fibres are treated with caffeine.

Transheterozygote females dncM14/rut2 and dncM14/rut3 deposit eggs that lack germ cells and display discrete morphological defects.

Mutants exhibit enhanced motor terminal aborization, this suggests cAMP levels influence the size of motor axon projections.

rut2 dncM14 flies learn about half as well as wild-type controls.

Eggs are laid.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference

dnc2/dncM14 has egg chamber phenotype, suppressible | partially by Gprk2[+]/Gprk206936

dncM14 has egg chamber phenotype, suppressible | partially by Gprk2[+]/Gprk206936

dncM14 has phenotype, suppressible by rut1

NOT suppressed by
Statement
Reference

dncM14/dnc221b has nurse cell | supernumerary phenotype, non-suppressible by rut[+]/rut2

dncM14/dnc221b has nurse cell & nucleus phenotype, non-suppressible by rut[+]/rut2

Suppressor of
Statement
Reference

dncM14 is a suppressor of NMJ bouton | supernumerary phenotype of sei2

dncM14 is a suppressor of neuromuscular junction phenotype of sei2

dncM14 is a suppressor of NMJ bouton | supernumerary phenotype of slo1

dncM14 is a suppressor of neuromuscular junction phenotype of slo1

dncM14/dnc[+] is a suppressor | partially of nurse cell & nucleus phenotype of Gprk206936

Additional Comments
Genetic Interactions
Statement
Reference

dncM14/+ suppresses the increase in bouton number per muscle area at the neuromuscular junction seen in larvae expressing FakdsRNA.Scer\UAS under the control of Scer\GAL4elav.PU.

A dncM14 mutant background severely suppresses the frequency of type B and type M satellites (small boutons) in dncM14; slo1 double mutants.

A dncM14 mutant background reduces the frequency of type M satellites and mature boutons in dncM14; sei2 double mutants.

The egg laying and hatching defects of Gprk206936 homozygous females are partially suppressed by a single copy of dncM14. The defect in tethering of the nurse cell nuclei seen in Gprk206936 homozygous egg chambers is partially suppressed by a single copy of dncM14. dncM14/dncM14 females carrying Gprk206936/+ lay 1.0 eggs/female/hour (wild-type value is 2.9 eggs/female/hour) of which 38.3% hatch. dnc2/dncM14 females carrying Gprk206936/+ lay 1.5 eggs/female/hour of which 31.6% hatch. The egg chamber degeneration of dncM14 homozygotes and dnc2/dncM14 females is partially suppressed by Gprk206936/+.

rut2 partially rescues the egg laying defects of dnc221b/dncM14, but fails to rescue the nurse cell nuclear morphology and 32-cell cyst defects.

Significantly suppresses the increase in average spontaneous miniature excitatory junctional current amplitude seen in larvae expressing Pka-R1BDK.Scer\UAS under the control of Scer\GAL4Mhc.PW.

Double mutants with stnA7 die within the pupal case.

The enhanced motor terminal aborization seen in dncM14 mutants is counterbalanced by addition of rut1. The effects of Sh and eag mutants can be potentiated by mutations in dnc and the enhancement can be counterbalanced by rut1. The effect of extreme hyperexcitability in eag Sh double mutants cannot be potentiated by mutations in dnc.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of dncScer\UAS.cCa exclusively in octopaminergic neurons under the control of Scer\GAL4Tdc2.PC rescues the increase in synaptopods at neuromuscular junction type II endings seen in dncM14 homozygous and dncM14/dncML heterozygous mutants.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Mohler.

Comments
Comments

Enzyme activity is 4% of wild-type.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (39)