Allele Dmel\Doa^HD

General Information
Symbol	Dmel\Doa^HD	Species	D. melanogaster
Name		FlyBase ID	FBal0002719
Feature type	allele	Associated gene	Dmel\Doa
Also Known As	Doa^HD1, Doa^HD2

Map ( GBrowse )
Allele class
Mutagen	PM hybrid dysgenesis, transposable element activity
Recent Updates
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FB2013_03	Genes Doa
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	PM hybrid dysgenesis transposable element activity (Rabinow and Birchler, 1989, Yun et al., 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References sequence variant 3R:24,715,654..24,716,869 linked_to=EcoRI-BamHI_rfrag comment=mapped to restriction fragment in Figure 4 of FBrf0074875, position of restriction fragment on reference sequence inferred by FlyBase curator evidence=experimental (Yun et al., 1994)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The copia{} element is inserted 177 nucleotides 3' to the first non-coding exon of Doa and 231 nucleotides 5' to the 55kD transcript's initiating AUG. (Kpebe and Rabinow, 2008) An uncharacterised 5kb transposable element in inserted within an intron. (Yun et al., 1994)
Caused by insertion	copia{}Doa^HD (Yun et al., 1994, Kpebe and Rabinow, 2008)
Cytology	Polytene chromosomes normal. (Rabinow and Birchler, 1989)
Phenotypic Data
Phenotypic Class
	chemical resistant \| dominant (James et al., 2009) lethal (with Df(3R)3450) (Yun et al., 2000) lethal (with Doa^01705b) (Yun et al., 2000) lethal \| recessive (Rabinow et al., 1993, Rabinow and Birchler, 1989) sex-determination defective (with Doa⁵) (Kpebe and Rabinow, 2008) visible \| dominant (Rabinow and Birchler, 1990)
Phenotype Manifest In

Detailed Description
	Statement Reference Doa[HD] heterozygous adults show significantly reduced sensitity to paraquat. (James et al., 2009) In Doa[HD]/Doa[s2784] mutants where the Doa[HD] allele is from the mother blackened areas are seen on the wings. When the Doa[s2784] allele comes from the mother the mutants die at or prior to the pupal stage. In Doa[HD]/Doa[5] mutants where the Doa[HD] allele is from the mother there is a strong transformation of females towards males, with male colouration of tergites 5 and 6, male sex combs on the prothoracic leg, and a normal but rotated female genital plate. When the Doa[5] allele comes from the mother there are no survivors. (Kpebe and Rabinow, 2008) Heterozygotes with Doa⁷ have ommatidia of varying size and disorganization of the interommatidial bristles. Random interruption of the normal lattice of pigment cells and random vacuolization of the retina and pigment cells are restored to at least normal levels (suppression of w^a). (Yun et al., 1994) Crosses between Doa^HD and Doa⁹ heterozygotes indicate that Doa is not an embryonic lethal, and is probably lethal during early larval stages. Rare Doa^HD/Doa⁶ or Doa^HD/Doa⁷ escapers of lethality are found at a frequency of approximately 0.5%. Doa^HD/Doa⁶ escapers are more frequently female than male (ratio 4:1) and the male escapers are sterile despite having motile sperm. (Rabinow and Birchler, 1989) Heterozygotes with Doa⁶ or Doa⁷ survive rarely and when they do, w^a is darkened to nearly wild-type color; w^sp55 reacts in the opposite manner, becoming nearly white; eyes of escapers have disarranged facets. Males of these heteroallelic combinations involving Doa⁶ are sterile despite having motile sperm; females and both sexes involving Doa⁷ are weakly fertile.
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Doa^HD, E(gl)3C^unspecified has fertile phenotype (Yun et al., 2000, Yun et al., 2000) Doa^HD, E(gl)3C^unspecified has viable phenotype (Yun et al., 2000, Yun et al., 2000) Doa^HD/Doa^Dem, Ef1γ^GE29510 has visible \| dominant phenotype (Fan et al., 2010)
Phenotype Manifest In
NOT Enhanced by
Statement Reference Doa⁷/Doa^HD has phenotype, non-enhanceable by gl² (Yun et al., 2000) Doa⁷/Doa^HD has phenotype, non-enhanceable by gl^60j (Yun et al., 2000) Doa⁷/Doa^HD has phenotype, non-enhanceable by Ras85D^e2F (Yun et al., 2000) Doa⁷/Doa^HD has phenotype, non-enhanceable by rl¹ (Yun et al., 2000) Doa⁷/Doa^HD has phenotype, non-enhanceable by rl^Sem (Yun et al., 2000) Doa⁷/Doa^HD has phenotype, non-enhanceable by sina¹ (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-enhanceable by Egfr^E1 (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-enhanceable by gl² (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-enhanceable by gl^60j (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-enhanceable by Ras85D^e2F (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-enhanceable by rl¹ (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-enhanceable by rl^Sem (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-enhanceable by sina¹ (Yun et al., 2000)
NOT suppressed by
Statement Reference Doa⁷/Doa^HD has phenotype, non-suppressible by gl² (Yun et al., 2000) Doa⁷/Doa^HD has phenotype, non-suppressible by gl^60j (Yun et al., 2000) Doa⁷/Doa^HD has phenotype, non-suppressible by Ras85D^e2F (Yun et al., 2000) Doa⁷/Doa^HD has phenotype, non-suppressible by rl¹ (Yun et al., 2000) Doa⁷/Doa^HD has phenotype, non-suppressible by rl^Sem (Yun et al., 2000) Doa⁷/Doa^HD has phenotype, non-suppressible by sina¹ (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-suppressible by Egfr^E1 (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-suppressible by gl² (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-suppressible by gl^60j (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-suppressible by Ras85D^e2F (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-suppressible by rl¹ (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-suppressible by rl^Sem (Yun et al., 2000) Doa^HD/Doa^Dem has phenotype, non-suppressible by sina¹ (Yun et al., 2000)
Enhancer of
Statement Reference Doa^HD is an enhancer of phenotype of w^sp55 (Rabinow and Birchler, 1989)
NOT Enhancer of
Statement Reference Doa^HD is a non-enhancer of phenotype of w^a2 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^a3 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^a4 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^a57i11 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^a58l (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^a59k1 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^apl (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^bf2 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^bf (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^bl (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^Bwx (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^co (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^col (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^e2 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^E (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^e (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^ec3 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^h (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of wⁱ (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^IR1 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^IR2 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^IR3 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^IR4 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^IR5 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^IR6 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^mo (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^r (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^sat (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^sp2 (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^sp81d (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of w^zm (Rabinow and Birchler, 1989) Doa^HD is a non-enhancer of phenotype of y² (Rabinow and Birchler, 1989)
Suppressor of
Statement Reference Doa^HD is a suppressor of phenotype of w^a (Rabinow and Birchler, 1989) Doa^HD is a suppressor of phenotype of w^aM (Rabinow and Birchler, 1989) Doa^HD is a suppressor of phenotype of w^aR84h (Rabinow and Birchler, 1989)
NOT Suppressor of
Statement Reference Doa^HD is a non-suppressor of phenotype of w^a2 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^a3 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^a4 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^a57i11 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^a58l (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^a59k1 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^apl (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^bf2 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^bf (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^bl (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^Bwx (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^co (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^col (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^e2 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^E (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^e (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^ec3 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^h (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of wⁱ (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^IR1 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^IR2 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^IR3 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^IR4 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^IR5 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^IR6 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^mo (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^r (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^sat (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^sp2 (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^sp81d (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of w^zm (Rabinow and Birchler, 1989) Doa^HD is a non-suppressor of phenotype of y² (Rabinow and Birchler, 1989)
Other
Statement Reference Doa^HD/Doa^Dem, Ef1γ^GE29510 has crossvein phenotype (Fan et al., 2010) Doa^HD/Doa^Dem, Ef1γ^GE29510 has eye phenotype (Fan et al., 2010) Doa^HD/Doa^Dem, Ef1γ^GE29510 has female genitalia phenotype (Fan et al., 2010) Doa^HD/Doa^Dem, Ef1γ^GE29510 has posterior crossvein \| male phenotype (Fan et al., 2010) Doa^HD/Doa^Dem, Ef1γ^GE29510 has wing phenotype (Fan et al., 2010) Doa^HD/Doa^Dem, Ef1γ^GE29510 has wing vein \| ectopic phenotype (Fan et al., 2010)
Additional Comments
Genetic Interactions
Statement Reference Doa[HD] Ef1γ[GE29510]/Doa[Dem] flies show a number of defects, including ectopic wing venation, failure of either of the crossveins to attach (particularly the posterior crossvein in males), aberrantly formed crossveins, occasional eye roughness, curved wings and rarely, partial rotation of the female genitalia. (Fan et al., 2010) Doa^HD in combination with E(gl)3C^unspecified results in flies with normal eyes, wings and bristles. No suppression of w^a is seen in these flies. (Yun et al., 2000) Rare Doa^HD/Doa⁶ or Doa^HD/Doa⁷ escapers show extreme suppression of the w^a phenotype, such that eyes, Malpighian tubules, ocelli and testes are essentially wild-type in colour. They have disordered eye facets and scutellar bristles are often missing or abnormal. Male escapers carrying w^sp55 have bleach-white eyes. (Rabinow and Birchler, 1989)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer
	The "HD1" and "HD2" alleles have an insertion in an identical location. They both potentially originate from a single event during mass crosses. (Yun et al., 1994)
Comments
	Does not interact with gypsy induced alleles at several loci (f¹, y² and ct⁶). (Rabinow and Birchler, 1989)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	Doa¹ Doa² Doa^HD1 (Yun et al., 1994, Rabinow et al., 1993, Rabinow and Birchler, 1990, Rabinow and Birchler, 1989, ) Doa^HD2 (Yun et al., 1994, Rabinow et al., 1993, Rabinow and Birchler, 1989) Doa^HD (James et al., 2009, Fan et al., 2010, Kpebe and Rabinow, 2008)
Name Synonym
Secondary FlyBase IDs
	FBal0002720
References ( 10 )
Research paper	Fan et al., 2010, Genetics 184(1): 141--154 Drosophila translational elongation factor-1{gamma} is modified in response to DOA kinase activity and is essential for cellular viability. [FBrf0209673] James et al., 2009, Free Radical Biol. Med. 46(6): 821--827 Superoxide dismutase is regulated by LAMMER kinase in Drosophila and human cells. [FBrf0207123] Kpebe and Rabinow, 2008, Genetics 179(4): 1973--1987 Dissection of darkener of apricot kinase isoform functions in Drosophila. [FBrf0205853] Morris et al., 2003, Genetics 164(4): 1435--1446 Identification and analysis of mutations in bob, Doa and eight new genes required for oocyte specification and development in Drosophila melanogaster. [FBrf0162144] Yun et al., 2000, Genetics 156(2): 749--761 The LAMMER protein kinase encoded by the Doa locus of Drosophila is required in both somatic and germline cells and is expressed as both nuclear and cytoplasmic isoforms throughout development. [FBrf0130184] Yun et al., 1994, Genes Dev. 8(10): 1160--1173 The Doa locus encodes a member of a new protein kinase family and is essential for eye and embryonic development in Drosophila melanogaster. [FBrf0074875] Rabinow et al., 1993, Genetics 134(4): 1175--1185 Mutations at the darkener of apricot locus modulate transcript levels of copia and copia-induced mutations in Drosophila melanogaster. [FBrf0058584] Rabinow and Birchler, 1990, Genet. Res. (Camb.) 55: 141--151 Interactions among modifiers of retrotransposon-induced alleles of the white locus of Drosophila melanogaster. [FBrf0051869] Rabinow and Birchler, 1989, EMBO J. 8: 879--889 A dosage-sensitive modifier of retrotransposon-induced alleles of the Drosophila white locus. [FBrf0049635]
Abstract	Staatz et al., 2005, A. Dros. Res. Conf. 46: 373A Doa regulates TrxR-1⁴⁸¹ and Sod-2. [FBrf0183505]

Allele Dmel\DoaHD

Allele Dmel\Doa^HD