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General Information
Symbol
Dmel\dor1
Species
D. melanogaster
Name
FlyBase ID
FBal0002729
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
deep orange1
Mutagen
Nature of the Allele
Mutagen
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

G1668297A

Amino acid change:

C979Y | dor-PA

Reported amino acid change:
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: C979Y.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutant males and females have a shortened adult life span compared to controls, with a mean life span of 8.96 and 10.07 days respectively at 25o.

Hemocyte endosomal degradation is significantly reduced in dor1 mutants compared to wild-type cells. This reduction also correlates with an increased severity of eye-colour defect (with increased severity in dor1 compared to dor4). Distinct from cells in wild-type and car1 animals, the large endosomes in mutant dor1 alleles appear blocked in progression to later stages. Endosomes in cells from dor1 mutants, although incapable of a morphological transition to small dense vesicles, are capable of fusion at early times and normal maturation.

Heterozygotes with T(1;2)dorvar7 are fully viable at 25oC but have reduced viability at 18oC. Heterozygotes have variegated eyes and show a range of morphological abnormalities including rough eyes, reduced bristles on the thorax and abnormal (wavy) wings. Eye phenotype is more extreme at 25oC and bristle phenotype is more extreme at 18oC.

PEV variegation induced by In(1LR)pn2a causes yellow spots on the eye surface. Variegating phenotype is suppressed by adding Y chromosome.

Morphological abnormalities: 3 types of dor-variegated eyes: 1) dark single facets or spots on the normal pigmented background. 2)dor-coloured sectors. 3) gradual transition from dark-pigmented area to dor-piment area. Position effect variegation results in crumpled, singed wings in 45-85% dor1/T(1;2)dorvar7 females.

Eye colour: orange at 25oC. viable

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhancer of
Statement
Reference
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The double mutant, car1 dor1 does not survive beyond the prepupal stage. In cell culture, car1 dor1 cells exhibit the most severe defect in endosomal degradation, compared to either single mutant. There is a small but significant difference between the degradation defect in dor1 car1 and dor1, consistent with a role for both car and dor in endosomal degradation. Endosomes in cells from car1 dor1 mutants, although incapable of a morphological transition to small dense vesicles, are capable of fusion at early times and normal maturation.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The eye colour phenotype of dor1 flies is rescued by dor1-1002.Scer\UAS or dor400-1002.Scer\UAS expressed under the control of Scer\GAL4GMR.PF. The eye colour phenotype of dor1 flies is not rescued by dorΔ401-800.Scer\UAS or dor1-802.Scer\UAS expressed under the control of Scer\GAL4GMR.PF, and the level of eye pigmentation in these flies is further reduced compared to dor1 single mutant flies.

Images (2)
Stocks (2)
Notes on Origin
Discoverer

E. D. King.

Comments
Comments

Strong dor mutation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
Reported As
Name Synonyms
deep orange1
Secondary FlyBase IDs
    References (15)