Open Close
General Information
Symbol
Dmel\dppd-blk
Species
D. melanogaster
Name
FlyBase ID
FBal0002996
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dppblk
Key Links
Allele class
Mutagen
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

Deletion from position 106 to 111.4 on the molecular map (FBrf0051824).

5kb deletion of sequences 17kb 3' of the dpp transcribed region.

Deletion spanning the disc region II and III, insertion of 1.9kb hobo element at the deletion site.

hobo

Insertion components
H{}dpp-d-blk
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Mutants have a very small, rough eye with few facets.

Homozygotes have small eyes.

Mutant show partial inhibition of the morphogenetic furrow, and reduced photoreceptor differentiation, resulting in a small eye phenotype in adults.

A morphogenetic furrow initiates only in the centre of mutant eye discs. Although it progresses anteriorly in a normal fashion, it fails to spread laterally. Homozygous flies have small eyes.

Small, rough eyes.

Ommatidial array is slightly disorganised but an equator is still discernible separating 10 or more rows of dorsal ommatidia from 2-3 rows of ventral ommatidia. Head cuticle replaces portions of the retinal field near the dorsal and ventral margins (the effect is more pronounced ventrally). Rotational polarity is maintained in dorsal and ventral portions. Asymmetric retinal differentiation occurs with retinal development (failure of ommatidial differentiation in the ventral eye disc), it is not a consequence of cell death. Anterior-dorsal ptcS2 clone in dppd-blk eye disc causes ectopic differentiation ahead of the morphogenetic furrow, this wave of ectopic differentiation propogates into neighbouring ptc+ tissue. A ventral margin clone in dppd-blk eye disc causes ectopic differentiation that propogates into ventral ptc+ tissue. Clone spanning a large portion of ventral epithelium and ventral posterior margin rescues ventral differentiation to a relatively normal pattern.

dally06464 homozygotes show a slight reduction in the eye which is more severe when they are also heterozygous for dppd-blk.

Homozygotes have greatly reduced compound eyes consisting of only a few ommatidia. The eye is largely replaced by frons cuticle, with ectopic frons cuticle lying between the orbital cuticle and the remaining ommatidia, and also between the shingle cuticle and ommatidia.

Eye discs have a similar appearance to those of hypomorphic Mad alleles. The furrow initiates in a restricted area at the posterior edge and dpp expression is not maintained along the posterior margin.

Allows furrow movement only in the central region of the eye disc, loss of one copy of wg is sufficient to increase the size of the eye.

Reduction in number of eye facets. Mutant phenotype can be rescued by P{TnJMB7}.

Eye reduced in size.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference

dppd-blk has visible phenotype, suppressible by l(2)rQ313[+]/snamarQ313

dppd-blk has visible phenotype, suppressible by snamaPX1/mnm[+]

Enhancer of
Statement
Reference

dppd-blk/dpp[+] is an enhancer of visible | recessive phenotype of dally06464

Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

dppd-blk has eye phenotype, enhanceable by trr1/trr[+]

dppd-blk has eye phenotype, enhanceable by B[+]/B1

dppd-blk has eye phenotype, enhanceable by osa308

Suppressed by
Statement
Reference

dppd-blk has eye phenotype, suppressible by l(2)rQ313[+]/snamarQ313

dppd-blk has ommatidium phenotype, suppressible by l(2)rQ313[+]/snamarQ313

dppd-blk has eye phenotype, suppressible by snamaPX1/mnm[+]

dppd-blk has ommatidium phenotype, suppressible by snamaPX1/mnm[+]

dppd-blk has eye phenotype, suppressible | partially by spenk13624/spenk07721

NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

dppd-blk/dpp[+] is an enhancer of eye phenotype of dally06464

Suppressor of
Statement
Reference

dppd-blk is a suppressor of phenotype of wgl-12

Other
Statement
Reference

dally[+]/dally06464, dppd-blk has eye phenotype

Additional Comments
Genetic Interactions
Statement
Reference

The dppd-blk phenotype is partially rescued by eyaScer\UAS.cBa expressed under the control of Scer\GAL4dpp.blk1. Specifically, the morphogenetic furrow spreads in a normal fashion dorsally along the lateral margin to form an almost complete dorsal half of an eye. The morphogenetic furrow does not spread ventrally along the margin. The small size of dppd-blk/dppd12 eye discs is partially rescued by eyaScer\UAS.cBa expressed under the control of Scer\GAL4dpp.blk1 and greater numbers of developing ommatidia are seen. The dppd-blk small eye phenotype is enhanced by soScer\UAS.cPa expressed under the control of either Scer\GAL4dpp.blk1 or Scer\GAL4ey.PH, which often results in a complete lack of eye development. Expression of dacScer\UAS.cSa under the control of Scer\GAL4ey.PH results in a variable decrease in the size of dppd-blk eyes. Expression of eyScer\UAS.cHa under the control of Scer\GAL4ey.PH interferes with dppd-blk eye development to a variable extent, often resulting in smaller eyes.

Flies heterozygous for both dally06464 and dppd-blk show eye abnormalities never observed in flies heterozygous for dally06464 or dppd-blk alone.

The eye disc phenotype is partially rescued by MadUbi-p63E.T:Hsap\MYC.

In wgl-12, dppd-blk eye discs the dppd-blk mutation completely abolishes the effects of loss of wg expression (inwardly directed furrow movement is not present).

In double mutants of bunrI043 and the eye specific allele dppd-blk the frequency of survivors is decreased relative to the single bunrI043 mutant and the eye is extremely reduced due to differentiation of a very small number of ommatidia.

Scer\GAL4dpp.blk1 driven expression of gbbScer\UAS.cSb or gbbScer\UAS.cSa is unable to rescue the mutant phenotype.

Transheterozygotes with eya4 are normal.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Small eye phenotype can be rescued by dppTnJMB7 and Scer\GAL4dpp.blk1 driving the expression of dppScer\UAS.cSa.

Allele class: d-blk

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

The strain dppd-blk is capable of promoting the mobilization of hobos. The strain contains numerous internally deleted hobos and two 3.0kb copies. The ability to promoter mobilisation results from the action of the 3.0kb elements. One of these elements was cloned and used to produce plasmid "pHFL1".

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (33)