Deletion of control elements.
Inversion breakpoint within the regulatory disk region, effectively deleting some of the regulatory sequences that direct expression in imaginal discs.
Breakpoint allele.
abnormal size (with dppd14)
viable (with dppTgR46.1)
visible (with dppd14), with Scer\GAL4dpp.blk1, dppMFSI.III.UAS.Tag:HA
visible (with dppd14), with Scer\GAL4dpp.blk1, dppMFSI.UAS.Tag:HA
visible (with dppd14), with Scer\GAL4dpp.blk1, dppUAS.cSa.Tag:HA
taste bristle (with dppd12)
wing vein L3 (with dppd14), with Scer\GAL4dpp.blk1, dppMFSI.UAS.Tag:HA
wing vein L3 (with dppd14), with Scer\GAL4dpp.blk1, dppUAS.cSa.Tag:HA
No wing phenotypes are seen in dppd6 heterozygotes.
dppd6/dppd14 flies are viable and have small wings.
dppd6/dppd14 wings are rescued by the expression of dppScer\UAS.cSa.T:Ivir\HA1 under the control of Scer\GAL4dpp.blk1 except for ectopic vein materials around longitudinal vein L3.
The size and morphology of dppd6/dppd14 mutant wings are almost completely rescued by the expression of dppMFSI.Scer\UAS.T:Ivir\HA1 via Scer\GAL4dpp.blk1 except for ectopic vein material around L3.
The size and morphology of dppd6/dppd14 mutant wings are almost completely rescued by the expression of dppMFSI.III.Scer\UAS.T:Ivir\HA1 via Scer\GAL4dpp.blk1 except for ectopic vein material around L3.
dppd6 mutant embryos display an increased number of hematopoietic cells in the lymph gland of embryos compared to wild-type, and the lymph gland is still hypertrophic in third instar larvae and there is an overabundance of plasmatocyte precursors. There is no significant difference in the number of crystal cell precursors in these larvae.
dppd6 mutant larvae fed on S. typhimurium experience a developmental delay of 1 full day from hatching to pupation, and infection results in eventual lethality of these animals. Under normal conditions, dppd6 mutant larvae generate 38% more blood cells per larvae compared to wild-type. There is significantly more (74%) blood cells observed in 3-day fed infected mutant larvae than in non-infected mutant larvae. There is no significant difference in the number of crystal cells between infected mutant and wild-type larvae. In the absence of infection 48% of blood cells in mutant larve are new plasmatocytes, compared to 31% in controls. Within 1 day of infection, the level of new plasmatocytes decreases to 23% and continues to decrease over subsequent days, reduced compared to levels in wild-type infected larvae. Infected dppd6 mutant larvae are significantly impaired in their ability to produce lamellocytes, whereas infected wild-type larvae show >10-fold increase in the percentage of circulating lamellocytes.
Stage 15 dppd6 embryos contain a significantly greater number of zfh1-expressing pericardial cells than normal, but show no change in the number of Mef2 expressing cardiac cells. Hyperplasia of the zfh1-expressing pericardial cells is still seen in stage 17 mutant embryos.
dppd6 embryos show expanded cell proliferation in the dorsal mesoderm during stage 12 compared to wild-type embryos, and cell division in the dorsal mesoderm persists in dppd6 embryos throughout stage 13 (in contrast to wild type, where cell division is absent in the dorsal mesoderm in stage 13).
In the anterior region of the heart (the aorta), mutant first instar larvae show no difference in heart rate or pulse distance compared to controls. However, in the posterior region (the heart proper), the mutant larvae show a considerable reduction in the systolic distance (mutant cells do not approach each other as closely as wild-type cells do) and the diastolic distance (mutant cells do not separate from each other as far as wild-type cells do). The mutant larvae have a 44% reduction in the average pulse distance per beat, an indication that the beat volume is significantly reduced. The heart rate in the posterior region of the heart is comparable to wild type.
Heterozygotes do not have an ectopic wing vein phenotype.
Approximately 50% of eggs laid by heterozygous females have shortened and abnormally shaped respiratory appendages.
dppd6/dppd12 flies have a reduced number of ommatidia. The adults are weak, short-lived (live for 2-3 days) and have abnormalities in the wing, leg, antenna and external genitalia. The tarsal and meta-tarsal segments of all legs are abnormal in dppd6/dppd12 flies, due to loss of claws and fusion of the tarsal segments. The dorsal parts of the leg are sometimes ventralised. The tibia and femur are progressively less affected, while the trochanter and coxa are almost wild type. The number of sex comb bristles is generally higher than normal. dppd6/dppd12 flies have defects in the distal segments of the antenna. The arista is usually absent and a conical projection is present on the third segment (which presumably represents the fused 4th, 5th and 6th antennal segments). Most dppd6/dppd12 males completely lack external genitalia while some have abnormal external genitalia. There is little effect on the female genitalia. The leg and antennal discs of dppd6/dppd12 larvae are deformed.
Small, rough eyes.
70 to 100% of homozygous males show loss of tarsi and claws and have small wings and eyes. 35 to 70% of homozygous males show duplication of leg structures, and 10 to 35% of homozygous males have duplicated antennal structures.
Homozygotes exhibit greatly reduced wings and eyes and loss of tarsal claws at the tips of the legs. Transheterozygotes with dpphr4 have wings approximately half size and normal legs and eyes. When in combination with Df(2L)C28/Mad+ the transheterozygotes exhibit more severe phenotype, further reduction in wing blade, slight reduction in the eye and loss of tarsal claws. Trisomy for Mad (Dp(2;3)JS20) partially ameliorates transheterozygote phenotype, wings are of normal size and show residual vein fusions.
The medulla neuropil is reduced to a size similar to that produced by inactivating wg prior to 44hr AEL. The number of ommatidia is correspondingly reduced. The number of outer proliferative center cells incorporating BrdU is markedly decreased in the Fas2 expressing domain of wg and dpp early third instar brains.
Adults lack distal portion of appendages.
dppd6/dpp[+] is a non-enhancer of visible phenotype of I-2UAS.Tag:polyHis,Tag:MYC, Scer\GAL4vg.PM, putUAS.cMa
dppd6/dpp[+] is a suppressor of abnormal neuroanatomy phenotype of spin10403
dppd6/dpp[+] is a suppressor | partially of visible phenotype of Scer\GAL4[-], SnooGS-C517T
dppd6/dpp[+] is a suppressor | partially of visible phenotype of Scer\GAL4Tub.PU, SnooGS-C517T
dppd6/dpp[+] is a suppressor of visible phenotype of Scer\GAL4Tub.PU, cswN308D.UASp
dppd6 is a suppressor of visible phenotype of upd1GMR.PB
dppd6/dpp[+] is a non-suppressor of visible phenotype of I-2UAS.Tag:polyHis,Tag:MYC, Scer\GAL4vg.PM, putUAS.cMa
Df(2R)en-SFX31/+, dppd6 has visible | dominant | adult stage phenotype
dppd6, en[+]/en54 has visible | dominant | adult stage phenotype
dpps4/dppd6, gbb1 has partially lethal phenotype
dpphr4/dppd6 has wing phenotype, enhanceable by tkvUAS.cNa/Scer\GAL4Tub.PU
dppd6/dppd12 has sex comb | increased number phenotype, enhanceable by B1
dppd6/dppd12 has tarsal segment phenotype, enhanceable by B1
dppd6/dppd12 has fourth segment of antenna phenotype, enhanceable by B1
dppd6/dppd12 has fifth segment of antenna phenotype, enhanceable by B1
dppd6/dppd12 has male genitalia phenotype, enhanceable by B1
dppd6/dppd12 has ommatidium phenotype, enhanceable by B1
dppd6 has tarsal segment phenotype, enhanceable by mxcG43
dpps4/dppd6 has anterior crossvein phenotype, suppressible by gbb1
dppd6 has eye phenotype, non-suppressible by gbbUAS.cSa/Scer\GAL4dpp.blk1
dppd6 has wing phenotype, non-suppressible by gbbUAS.cSa/Scer\GAL4dpp.blk1
dppd6 has eye phenotype, non-suppressible by gbbUAS.cSb/Scer\GAL4dpp.blk1
dppd6 has wing phenotype, non-suppressible by gbbUAS.cSb/Scer\GAL4dpp.blk1
dpphr4/dppd6 is an enhancer of wing phenotype of Scer\GAL4Tub.PU, tkvUAS.cNa
dppd6 is an enhancer of anterior crossvein phenotype of cv-2k03511/Df(2R)Pu-D17
dppd6/dpp[+] is a non-enhancer of wing phenotype of I-2UAS.Tag:polyHis,Tag:MYC, Scer\GAL4vg.PM, putUAS.cMa
dppd6/dpp[+] is a suppressor of glial cell phenotype of spin10403
dppd6/dpp[+] is a suppressor | partially of wing vein | ectopic phenotype of Scer\GAL4Tub.PU, SnooGS-C517T
dppd6/dpp[+] is a suppressor | partially of wing vein | ectopic phenotype of Scer\GAL4[-], SnooGS-C517T
dppd6/dpp[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL4Tub.PU, cswN308D.UASp
dppd6 is a suppressor of eye phenotype of upd1GMR.PB
dppd6 is a non-suppressor of embryonic/larval lymph gland | embryonic stage phenotype of zfh12
dppd6 is a non-suppressor of hemocyte | embryonic stage phenotype of zfh12
dppd6/dpp[+] is a non-suppressor of wing phenotype of I-2UAS.Tag:polyHis,Tag:MYC, Scer\GAL4vg.PM, putUAS.cMa
dppd6, ix1 has terminalia phenotype
dppd5/dppd6, ix1 has terminalia phenotype
dppd-ho/dppd6, ix1 has terminalia phenotype
Df(2R)en-SFX31/+, dppd6 has anterior crossvein phenotype
dppd6, en[+]/en54 has anterior crossvein phenotype
B1, dppd6/dppd12 has antennal segment 2 sense organ phenotype
B1, dppd6/dppd12 has second segment of antenna phenotype
B1, dppd6/dppd12 has antennal segment 3 sense organ phenotype
B1, dppd6/dppd12 has third segment of antenna phenotype
B1, dppd6/dppd12 has leg sensillum phenotype
dppd5/dppd6, sax1 has prothoracic unguis phenotype
dppd5/dppd6, sax1 has mesothoracic unguis phenotype
dppd5/dppd6, sax1 has metathoracic unguis phenotype
dppd5/dppd6, sax2 has prothoracic unguis phenotype
dppd5/dppd6, sax2 has mesothoracic unguis phenotype
dppd5/dppd6, sax2 has metathoracic unguis phenotype
Loss of the anterior crossvein is seen in 74% of dppd6 Df(2R)en-SFX31 double heterozygous flies.
Loss of the anterior crossvein is seen in dppd6 en54 double heterozygous flies.
The frequency of the SnooGS-C517T/SnooGS-C517T ectopic wing vein phenotype is significantly suppressed if the flies are also carrying one copy of dppd6.
The frequency of the ectopic wing vein phenotype caused by expression of SnooGS-C517T under the control of Scer\GAL4tub is significantly suppressed if the flies are also carrying one copy of dppd6.
Flies that express tkvScer\UAS.cNa, under the control of Scer\GAL4tub, in a dpphr4/dppd6 background, have smaller wings than flies expressing the transgene in a wild-type background and than dpphr4/dppd6 flies that do not express tkvScer\UAS.cNa.
The severity of the leg defects seen in dppd6/dppd16 flies is enhanced by B1. There is an overall shortening in length along the proximal-distal axis of the leg and complete or partial fusion or even total loss of tarsal segments. The tibia and femur are deformed, showing shortening, bulging and disorganised bristle patterns. The coxa and trochanter are not so strongly affected. The sex comb of the prothoracic leg, when present, is always duplicated in these flies. The antenna phenotype of dppd6/dppd16 flies is enhanced by B1; the arista, 4th and 5th segments are completely absent and the 2nd and 3rd segments are widened and deformed and have defects in the bristle pattern. B1 ; dppd6/dppd16 flies completely lack ommatidia. All B1 ; dppd6/dppd16 males completely lack external genitalia.
Very few wgAct5C.PS; dppd6/dppd8 flies exhibit wg-induced transdetermination, legs with wing tissue.
Scer\GAL4dpp.blk1 driven expression of gbbScer\UAS.cSa or gbbScer\UAS.cSb is unable to rescue the mutant phenotype.
dppd6 is rescued by dppUAS.cSa/Scer\GAL4dpp.blk1
dppd6/dppd14 is partially rescued by Scer\GAL4dpp.blk1/dppUAS.cSa.Tag:HA
dppd6/dppd14 is partially rescued by Scer\GAL4dpp.blk1/dppMFSI.UAS.Tag:HA
dppd6/dppd14 is partially rescued by dppMFSI.III.UAS.Tag:HA/Scer\GAL4dpp.blk1
dppd6/dppd14 is not rescued by dppMFSII.UAS.Tag:HA
dppd6/dppd14 wings are rescued by the expression of dppScer\UAS.cSa.T:Ivir\HA1 under the control of Scer\GAL4dpp.blk1 except for ectopic vein materials around longitudinal vein L3.
The size and morphology of dppd6/dppd14 mutant wings are almost completely rescued by the expression of dppMFSI.Scer\UAS.T:Ivir\HA1 via Scer\GAL4dpp.blk1 except for ectopic vein material around L3.
The size and morphology of dppd6/dppd14 mutant wings are almost completely rescued by the expression of dppMFSI.III.Scer\UAS.T:Ivir\HA1 via Scer\GAL4dpp.blk1 except for ectopic vein material around L3.
The expression of dppMFSII.Scer\UAS.T:Ivir\HA1 via Scer\GAL4dpp.blk1 fails to rescue the wing phenotype of dppd6/dppd14 mutants.
Eye and wing phenotypes can be rescued by Scer\GAL4dpp.blk1 driving the expression of dppScer\UAS.cSa.
Belongs to the disk-III class of alleles.
Allele class: d-III
dpp class III allele.
Clonal analysis shows a requirement for dpp+ function in cells adjacent to the anterior-posterior compartment boundary of the wing primordium.
Intermediate disk dpp allele.