FlyBase Allele Report: Dmel\dpp[d8]

General Information

Symbol

Dmel\dpp^d8

Species

D. melanogaster

Name

FlyBase ID

FBal0003005

Feature type

allele

Associated gene

Dmel\dpp

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

hypomorphic allele - genetic evidence

Mutagen

X ray

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Jazwinska et al., 1999)

Mutagen

X ray

Progenitor genotype

Caused by aberration

In(2L)dpp^d8

Cytology

Description

Breakpoint allele.

(St. Johnston et al., 1990)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal size | adult stage (with dpp^d12), with Ecol\lexA^LG.dpp, Scer\GAL4^hh.PU, Zzzz\VHH^{UAS.morphotrap.ext.mCherry}, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

abnormal size | adult stage (with dpp^d12), with Ecol\lexA^LG.dpp, Zzzz\VHH^{lexAop.morphotrap.ext.mCherry}, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

abnormal size | third instar larval stage (with dpp^d12), with Ecol\lexA^LG.dpp, Scer\GAL4^hh.PU, Zzzz\VHH^{UAS.morphotrap.ext.mCherry}, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

abnormal size | third instar larval stage (with dpp^d12), with Ecol\lexA^LG.dpp, Zzzz\VHH^{lexAop.morphotrap.ext.mCherry}, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

visible

(Maves and Schubiger, 1998, Lecuit et al., 1996, St. Johnston et al., 1990)

visible (with dpp^d12)

(Teleman and Cohen, 2000)

visible (with dpp^hr56)

(Adachi-Yamada et al., 1999)

visible | adult stage (with dpp^d12)

(Harmansa et al., 2015)

visible | adult stage (with dpp^d12), with Ecol\lexA^LG.dpp, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

visible | adult stage (with dpp^d12), with Ecol\lexA^LG.dpp, Scer\GAL4^hh.PU, Zzzz\VHH^{UAS.morphotrap.ext.mCherry}, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

visible | adult stage (with dpp^d12), with Ecol\lexA^LG.dpp, Zzzz\VHH^{lexAop.morphotrap.ext.mCherry}, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

visible | recessive

(Emerald and Roy, 1998)

Phenotype Manifest In

alar lobe

(Lecuit et al., 1996)

eye (with dpp^d10)

(Sopory, 2006)

hypogynial tooth

(Emerald and Roy, 1998)

leg

(Maves and Schubiger, 1998, St. Johnston et al., 1990)

male genitalia

(Emerald and Roy, 1998)

wing

(Maves and Schubiger, 1998, Lecuit et al., 1996)

wing (with dpp^d10)

(Sopory, 2006)

wing (with dpp^d12)

(Teleman and Cohen, 2000, Jazwinska et al., 1999)

wing (with dpp^hr56)

(Adachi-Yamada et al., 1999)

wing blade (with dpp^d12)

(Harmansa et al., 2015)

wing blade (with dpp^d12), with Ecol\lexA^LG.dpp, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

wing blade (with dpp^d12), with Ecol\lexA^LG.dpp, Scer\GAL4^hh.PU, Zzzz\VHH^{UAS.morphotrap.ext.mCherry}, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

wing blade (with dpp^d12), with Ecol\lexA^LG.dpp, Zzzz\VHH^{lexAop.morphotrap.ext.mCherry}, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

wing disc | third instar larval stage (with dpp^d12), with Ecol\lexA^LG.dpp, Scer\GAL4^hh.PU, Zzzz\VHH^{UAS.morphotrap.ext.mCherry}, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

wing disc | third instar larval stage (with dpp^d12), with Ecol\lexA^LG.dpp, Zzzz\VHH^{lexAop.morphotrap.ext.mCherry}, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

wing vein (with dpp^d12), with Ecol\lexA^LG.dpp, dpp^{lexAop.cHa.EGFP}

(Harmansa et al., 2015)

Detailed Description

Statement

Reference

The wings of dpp^d8/dpp^d12 mutants do not develop at all, only rudimentary stubs are formed, this can be rescued by expression of Ecol\lexA^LG.dpp>dpp^{lexAop.cHa.EGFP} : the wings of these adults display venation defects (extra vein material at the anterior crossvein, loss of distal part of L4) and the have slightly elongated shape but their sizes are comparable to that of control wings. The wing size of these rescued flies can in turn be reduced again by hindering the spreading of the dpp morphogen: completely blocking it by co-expressing Zzzz\vhhGFP4^{Ecol\lexAop.morphotrap.ext.T:Disc\RFP-mCherry} at the source, i.e. within the dpp expression domain or reducing it by expressing Zzzz\vhhGFP4^{Scer\UAS.morphotrap.ext.T:Disc\RFP-mCherry} under the control of Scer\GAL4^hh.PU (i.e. in the posterior compartment). The former blocks the wing blade development completely, with the latter the blade size is strongly decreased and the patterning in its posterior part is lost.

Blocking dpp spreading at its source however does not interfere with the uniform proliferation pattern in larval imaginal discs as the density of mitotic cells (pH3-positive) is comparable between the rescue and morphotrap discs. Clonal growth rates are also not generally affected (assessed by utilizing the Raeppli tissue-labelling tool), but in the morphotrap wing discs low numbers of very small clones (1-3) are found next to the A/P boundary and these are not observed in the control discs.

In wing discs where dpp spreading is blocked within the dpp expression domain, the growth of the lateral part of the wing compartments shows similar width increase and similar growth rate as in the control discs with dpp spreading unhindered. However, the proliferation of the medial cell population (marked by low brk) is abolished, so the overall size of wing disc is visibly decreased compared to controls.

Similarly, hindering the dpp spreading at its (anterior) source reduces the size of the posterior wing pouch by more than 60%, blocking the dpp spreading in the posterior compartment (by expressing the morphotrap under the control of Scer\GAL4^hh.PU) decreased the posterior pouch size by about 40%.

(Harmansa et al., 2015)

dpp^d8/dpp^d10 mutants exhibit small eyes and reduced or absent wings.

(Sopory, 2006)

The wing blade is almost entirely missing in dpp^d8/dpp^d12 animals. Some wing hinge structures are present.

(Teleman and Cohen, 2000)

dpp^d8/dpp^hr56 flies show a small notching of the wing tip in less than 10% of cases.

(Adachi-Yamada et al., 1999)

dpp^d8/dpp^d12 flies have rudimentary wings.

(Jazwinska et al., 1999)

The external genitalia of dpp^d8/dpp^d12 males are reduced to a small ring like structure. dpp^d8/dpp^d12 females show a complete duplication of the thorn bristles of the vaginal plate.

(Emerald and Roy, 1998)

dpp^d6/dpp^d8 transheterozygotes have defects in both wing and leg development. Mutants can produce ventral wing hinge structures in transdetermined legs.

(Maves and Schubiger, 1998)

dpp^d8/dpp^d12 dics wing pouch is small, almost no wing tissue remains, except structures for the proximal hinge region. The proximal costa remains in the anterior compartment and a reduced alula in the posterior. Partial rescue of the wing phenotype is achieved by tkv^{Q253D.Scer\UAS.cLa} expression driven by Scer\GAL4^dpp.blk1.

(Lecuit et al., 1996)

Adults lack distal portion of appendages.

(St. Johnston et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

dpp^hr56/dpp^d8 has visible phenotype, enhanceable by puc^E69/puc[+]

(Adachi-Yamada et al., 1999)

dpp^hr56/dpp^d8 has visible phenotype, enhanceable by hep[+]/hep^CA

(Adachi-Yamada et al., 1999)

Enhancer of

Statement

Reference

dpp^d8 is an enhancer of visible phenotype of bi¹

(Grimm and Pflugfelder, 1996)

Phenotype Manifest In

Enhanced by

Statement

Reference

dpp^hr56/dpp^d8 has wing phenotype, enhanceable by puc^E69/puc[+]

(Adachi-Yamada et al., 1999)

dpp^hr56/dpp^d8 has wing phenotype, enhanceable by hep[+]/hep^CA

(Adachi-Yamada et al., 1999)

Enhancer of

Statement

Reference

dpp^d8 is an enhancer of wing phenotype of bi¹

(Grimm and Pflugfelder, 1996)

NOT Suppressor of

Statement

Reference

dpp^d8 is a non-suppressor of wing phenotype of Scer\GAL4^71B, tkv^CA.UAS

(Adachi-Yamada et al., 1999)

Additional Comments

Genetic Interactions

Statement

Reference

The dpp^d8/dpp^hr56 phenotype is significantly enhanced by a single copy of puc^E69 or hep^CA, and in the case of hep^CA a wide region of the wing blade is lost, leaving only the base of the wing.

(Adachi-Yamada et al., 1999)

brk^M68 clones induced in a dpp^d8/dpp^d12 background lead to outgrowths of wing blade material indicating that normal target gene activation occurs without normal dpp levels.

(Jazwinska et al., 1999)

Very few wg^Act5C.PS; dpp^d6/dpp^d8 flies exhibit wg-induced transdetermination, legs with wing tissue.

(Maves and Schubiger, 1998)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

dpp^d10/dpp^d8 is rescued by dpp^{UAS.Tag:HA,Tag:MYC}/Scer\GAL4^dpp.blk1

(Sopory, 2006)

Partially rescued by

dpp^d10/dpp^d8 is partially rescued by dpp^{S2KK.UAS.Tag:HA,Tag:MYC}/Scer\GAL4^dpp.blk1

(Sopory, 2006)

dpp^d12/dpp^d8 is partially rescued by dpp^UAS.T.EGFP/Scer\GAL4^dpp.blk1

(Teleman and Cohen, 2000)

Comments

dpp^{Scer\UAS.T.T:Avic\GFP-EGFP} rescues the wing phenotype of dpp^d8/dpp^d12 flies when expressed under the control of Scer\GAL4^dpp.blk1; wing size and pattern are almost normal except that ectopic vein material is seen near vein 3 and the spacing between veins 3 and 4 is slightly increased.

(Teleman and Cohen, 2000)

Belongs to the disk-III class of alleles.

(St. Johnston et al., 1990)

Allele class: d-III

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Comments

dpp class III allele.

(Segal and Gelbart, 1985)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

dpp⁸

(Segal and Gelbart, 1985)

dpp^d8

(Simon et al., 2024, Matsuda et al., 2021, Zecca and Struhl, 2021, Harmansa et al., 2017, Harmansa et al., 2015, Wartlick et al., 2011, Bollenbach et al., 2008, Sopory, 2006, Grimm and Pflugfelder, 1996)

Name Synonyms

Secondary FlyBase IDs

References (20)

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