|Feature type||allele||Associated gene||Dmel\dpp|
|Also Known As||dpp12|
|Allele class||hypomorphic allele - genetic evidence|
What does this section display?
This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms).
What does this section not display?
This section does not currently display links that were removed or gene model changes.
Click the icon below to subscribe to this FlyBase record and receive updates automatically through your feed reader.
|All updates||Click here to see a list of all updates to this record from FB2010_08 and on.|
|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
Deletion of control elements.
Disrupts 3' regions controlling disc expression.
|Caused by aberration|
|Phenotype Manifest In|
The wings of dppd5/dppd12 mutants are so small that the identification of patterning defects is obscured.
dppd12/dppd6 flies show a considerable loss of taste bristles but do not show a loss of pseudotrachae or a significant change in gross mediproboscis morphology.
dppd5/dppd12 females have normal vaginal plates and tergite eight, showing discretely reduced analia in a few cases. dppd5/dppd12 males have extensively reduced terminalia and the penis apparatus can be duplicated or triplicated.
The wing blade is almost entirely missing in dppd8/dppd12 animals. Some wing hinge structures are present.
dppd6/dppd12 flies have a reduced number of ommatidia. The adults are weak, short-lived (live for 2-3 days) and have abnormalities in the wing, leg, antenna and external genitalia. The tarsal and meta-tarsal segments of all legs are abnormal in dppd6/dppd12 flies, due to loss of claws and fusion of the tarsal segments. The dorsal parts of the leg are sometimes ventralised. The tibia and femur are progressively less affected, while the trochanter and coxa are almost wild type. The number of sex comb bristles is generally higher than normal. dppd6/dppd12 flies have defects in the distal segments of the antenna. The arista is usually absent and a conical projection is present on the third segment (which presumably represents the fused 4th, 5th and 6th antennal segments). The leg and antennal discs of dppd6/dppd12 larvae are deformed. Most dppd6/dppd12 males completely lack external genitalia while some have abnormal external genitalia. There is little effect on the female genitalia.
The external genitalia of dppd6/dppd12 and dppd8/dppd12 males are reduced to a small ring like structure. The external genitalia of dpp10638/dppd12 males are highly defective and the penis apparatus is less defective. dppd6/dppd12 and dpp10638/dppd12 females show duplication of the thorn bristles accompanied by an absence of long bristles. dppd8/dppd12 females show a complete duplication of the thorn bristles of the vaginal plate.
Heterozygotes are phenotypically wild-type.
Homozygous clones induced in first instar larvae produce abnormalities in the dorsal side of the leg. Pattern elements from the dorsal side are missing, and may or may not be replaced with a duplicate version of the remaining ventral part of the leg. Duplications are arranged as mirror images. Homozygous clones doubly mutant for dppd12 and wgl-17 show defects in both the ventral and dorsal sides of the leg. The frequency of duplications is reduced compared to the single mutant homozygous dppd12 or wgl-17 clones.
dppd8/dppd12 dics wing pouch is small, almost no wing tissue remains, except structures for the proximal hinge region. The proximal costa remains in the anterior compartment and a reduced alula in the posterior. Partial rescue of the wing phenotype is achieved by tkvQ253D.Scer\UAS.cLa expression driven by Scer\GAL4dpp.blk1.
Heterozygotes with dppd5 have reduced wings. dppd5/dppd12 heterozygotes have reduced wings. Some dppd12 ptcG20/dppd5 ptc16 individuals eclose and show a partial rescue of the ptc phenotype (decrease in the numbers of bristles on the notum, legs and antennae and number of teeth in the male sex comb and a reduction in size of overgrown structures).
A posterior clone runs along the antero-posterior boundary until it reaches a gap in wing vein 4 (a region sensitive to lack of dpp) where the border of the clone becomes irregular.
medulla neuropil ommatidia In dppd12/dppd6 or dppd12/dppd14 flies the medulla neuropil is reduced to a size similar to that produced by inactivating wg prior to 44hr AEL. The number of ommatidia is correspondingly reduced.
Rudimentary imaginal discs.
|NOT Enhancer of|
dppd12 is a suppressor of increased cell number | somatic clone phenotype of BacA\p35Scer\UAS.cHa, Scer\GAL4tub.PU
|NOT Suppressor of|
dppd12/dpp[+] is a non-suppressor of large body phenotype of Scer\GAL429BD, dallysec.Scer\UAS.T:Hsap\MYC
dppd12/dpp[+] is a non-suppressor of visible phenotype of I-2Scer\UAS.T:Zzzz\His6,T:Hsap\MYC, Scer\GAL4vg.PM, putScer\UAS.cMa
dppd12 is a non-suppressor of cell death defective | somatic clone phenotype of BacA\p35Scer\UAS.cHa, Scer\GAL4tub.PU
dppd12 is a non-suppressor of planar polarity defective phenotype of Scer\GAL4hs.2sev, Tak1Scer\UAS.cMa
|Phenotype Manifest In|
|NOT Enhancer of|
dppd12/dpp[+] is a non-enhancer of wing phenotype of I-2Scer\UAS.T:Zzzz\His6,T:Hsap\MYC, Scer\GAL4vg.PM, putScer\UAS.cMa
|NOT Suppressor of|
dppd12/dpp[+] is a non-suppressor of wing phenotype of I-2Scer\UAS.T:Zzzz\His6,T:Hsap\MYC, Scer\GAL4vg.PM, putScer\UAS.cMa
The glial overmigration phenotype seen in spin eye discs is significantly suppressed if the animals are also heterozygous for dpp[d12].
sax/+ enhances the trans-heterozygous dpp[d12] Df(2L)JS17 phenotype characterised by missing tarsal claws and abnormal wing venation.
gbb1, dppd12/+ clones induced in the anterior of the wing leads to a greater loss of the L4-L5 intervein than gbb1 clones but no greater loss of the L5 wing vein. The wing pouch of dppd12/dpphr56 wing discs is reduced to essentially a dorsal/ventral margin with no detectable vein primordia.
dppd12 heterozygosity does not reduce animal size when dallysec.Scer\UAS.T:Hsap\MYC is driven by Scer\GAL429BD.
The small size of dppd-blk/dppd12 eye discs is partially rescued by eyaScer\UAS.cBa expressed under the control of Scer\GAL4dpp.blk1 and greater numbers of developing ommatidia are seen.
brkM68 clones induced in a dppd8/dppd12 background lead to outgrowths of wing blade material indicating that normal target gene activation occurs without normal dpp levels.
The severity of the leg defects seen in dppd6/dppd16 flies is enhanced by B1. There is an overall shortening in length along the proximal-distal axis of the leg and complete or partial fusion or even total loss of tarsal segments. The tibia and femur are deformed, showing shortening, bulging and disorganised bristle patterns. The coxa and trochanter are not so strongly affected. The sex comb of the prothoracic leg, when present, is always duplicated in these flies. The antenna phenotype of dppd6/dppd16 flies is enhanced by B1; the arista, 4th and 5th segments are completely absent and the 2nd and 3rd segments are widened and deformed and have defects in the bristle pattern. B1 ; dppd6/dppd16 flies completely lack ommatidia. All B1 ; dppd6/dppd16 males completely lack external genitalia.
The gbb4/gbb4 and gbb1/gbb4 phenotypes are dominantly enhanced by dppd12; there is a greater loss of wing vein L4, wing vein L2 and the anterior crossvein than in gbb single mutant flies. There is also loss of intervein tissue, especially between veins L2 and L3 and between veins L4 and L5, resulting in a reduction in the size of the wing. A gap at the distal end of vein L3 is seen in 3% of cases. A reduction in the loss of wing vein L5 is seen in these flies. Viability is reduced. gbb1 dppd12/gbb4 + flies show truncations and/or fusions of the distal-most tarsal segments of the male prothoracic leg.
X-ray irradiated wing disc clones expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub in dppd12 mutants contain a mixture of two types of cell: `live' cells, which have not initiated apoptosis due to the irradiation, and `undead' cells (between 20 and 70% of the clone), which have initiated apoptosis, but have failed to complete programmed cell death. No such cells persist outside the clones in which BacA\p35Scer\UAS.cHa is expressed. X-ray irradiated wing disc clones expressing BacA\p35Scer\UAS.cHa under the control of Scer\GAL4tub in dppd12 wgl-17 mutants contain a mixture of two types of cell: `live' cells, which have not initiated apoptosis due to the irradiation, and `undead' cells (between 20 and 70% of the clone), which have initiated apoptosis, but have failed to complete programmed cell death. No such cells persist outside the clones in which BacA\p35Scer\UAS.cHa is expressed. Unlike BacA\p35Scer\UAS.cHa (Scer\GAL4tub) wing disc clones, neither BacA\p35Scer\UAS.cHa (Scer\GAL4tub) dppd12 nor BacA\p35Scer\UAS.cHa (Scer\GAL4tub) dppd12 wgl-17 clones appear to be associated with extra proliferation or growth after stress (such as X-ray irradiation).
|Complementation & Rescue Data|
|Partially rescued by|
|Not rescued by|
Expression of dppαTub84B.PG in clones in the male terminalia in a dppd5/dppd12 background results in the recovery of terminal structures. The degree of recovery depends on the developmental stage at which the clone is induced. Recovery of terminal structures is accompanied by reduced penis apparatus duplications. The rescue is non-autonomous.
dppScer\UAS.T.T:Avic\GFP-EGFP rescues the wing phenotype of dppd8/dppd12 flies when expressed under the control of Scer\GAL4dpp.blk1; wing size and pattern are almost normal except that ectopic vein material is seen near vein 3 and the spacing between veins 3 and 4 is slightly increased.
Belongs to the disk-V class of alleles.
Allele class: d-V
|Stocks ( 3 )|
|Notes on Origin|
Lacks disc function and retains shortvein function.
Two copies fail to rescue defects of class V heterozygote dppd12/dppd14, individuals fail to form cuticle, most do not eclose from the pupal case, those that do have near full size wings and some legs are missing tarsal segments.
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 5 )|
|Secondary FlyBase IDs|
|References ( 65 )|
|Generate a list of|
|List References by type|
|Recent research papers ( 2 )|