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General Information
Symbol
Dmel\dpphr27
Species
D. melanogaster
Name
FlyBase ID
FBal0003067
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
dpp27, dpphin-r27
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
G2457739A
Reported nucleotide change:
G2133A
Amino acid change:
E316K | dpp-PA; E316K | dpp-PB; E316K | dpp-PC; E316K | dpp-PE
Reported amino acid change:
E316K
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Polytene chromosomes normal.
Nature of the lesion
Statement
Reference
Amino acid replacement: E316K.
Mutation falls in the N-terminal pro region of the dpp protein.
Nucleotide substitution: G2133A.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
dppd6/dpphr27 mutants have wings that are significantly reduced in size and that are missing L3 and L5.
dpphr27/dpphr56 males that have been raised at 18oC and then shifted to the restrictive temperature of 29oC upon eclosion do not have any overt morphological abnormalities in the testes after 7 days at 29oC and the testes contain germ cells in all stages and in quantities indistinguishable from that of controls.
Approximately 50% of eggs laid by heterozygous females have shortened and abnormally shaped respiratory appendages.
dppd6/dpphr27 flies show partial fusions of the L2 and L3 and the L4 and L5 wing veins. The wing is compressed along the A/P axis compared to wild type.
The distance between veins L4 and L5 is reduced in dppd5/dpphr27 flies.
Embryos are ventralised.
Embryos show cuticular holes, disorganization of the head skeleton, internalization of the seventh and eighth abdominal segments and the filzkorper and a thoracic constriction. The first and second ventral denticle belts are expanded, particularly at the tips. The filzkorper are fused into a single globular mass, reflecting the loss of dorsal fates that keeps the two anlage distinct in wild type. A significant percentage of surviving transheterozygotes between dpphr56 and dpphr4 or dpphr27 have a split notum.
Survival of heterozygotes is reduced if also heterozygous for certain alleles of tld.
dpphr27/+ females that are also sax1/+, sax2/+ or tkv7/+ (derived from dpphr27/+ males) are generally inviable.
The presence of tkv7 in the mother causes dpphr27 to behave as a dominant lethal in the zygote.
Embryos display a moderately ventralised phenotype and the mutation has an haploinsufficient effect, a small fraction of heterozygous zygotes die before reaching adulthood. Maternally contributed tkv alleles cause lethality.
Clonal analysis in the developing eye (using the FLP/FRT system) revealed nonautonomous phenotypes with large clones showing posterior-lateral eye parts missing.
Dominant lethality less than 50%. Homozygous and transheterozygous embryos were examined with respect to 25 cuticular markers, results demonstrate a graded requirement for dpp along the dorso-ventral axis.
Moderate ventralised phenotype. Rings of ventral denticle belts differentiate around the entire dorsoventral axis, almost no dorsal hairs are seen and the antennal and maxillary sense organs are missing. Defective movements of the germ band: due to loss of the amnioserosa and because the dorsalmost cells have acquired the lateral fate of the dorsal ectoderm. Dorsal cell fates are deleted and ventrolateral mitotic domains are expanded.
Heterozygotes with class I dpp alleles have a wild type wing phenotype. Heterozygotes with class II allele dppd5 exhibit wings with a deletion of the anterior crossvein. Heterozygotes with class II allele dppd28 exhibit held out wings that are missing longitudinal and cross veins, deletion of tarsal claws, male terminalia and female analia. Heterozygotes with class III dpp alleles display reduced held out wings that are missing longitudinal and cross veins, capitella-less halteres, reduced eyes, legs missing claws and missing or rotated male terminalia or female analia. Heterozygotes with class IV dpp alleles display the same phenotype as class III heterozygotes with duplication of scutellar bristles, medial cleft of the dorsal notum, duplication of sex combs and partial duplication of the third antennal segment. Heterozygotes with class V dpp alleles rarely eclose, the surviors exhibit the wing, haltere, eye and terminalia defects. At 18oC the heterozygotes have less severe phenotypes.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by MedUASp.cSa
Other
Statement
Reference
Mad12/Mad[+], dpphr27 has lethal | dominant phenotype
Med[+]/Med1, dpphr27 has lethal | dominant phenotype
dpphr27, sax[+]/sax1 has lethal | dominant phenotype
dpphr27, sax[+]/sax2 has lethal | dominant phenotype
dpphr27/dpp[+], sax2 has lethal | dominant phenotype
dpphr27/dpp[+], sax1 has lethal | dominant phenotype
Phenotype Manifest In
Enhanced by
Statement
Reference
dpphr27 has phenotype, enhanceable by Df(3L)66C-G28/+
dpphr27 has phenotype, enhanceable by vri5R7.2
dpphr27 has phenotype, enhanceable by vri5R8.4
dpphr27 has phenotype, enhanceable by vrik03801
dpphr27 has phenotype, enhanceable by vrik05901
dpphr27 has phenotype, enhanceable by vrik09602
dpphr27 has phenotype, enhanceable by vrik09713
dpphr27 has phenotype, enhanceable by vrik11805
dpphr27 has phenotype, enhanceable by vri5R4.11
dpphr27 has phenotype, enhanceable by vri5R5.24
dpphr27 has phenotype, enhanceable by vri5R5.7
dpphr27 has phenotype, enhanceable by tld14
NOT Enhanced by
Statement
Reference
dppSal20, dpphr27 has phenotype, non-enhanceable by Df(3L)66C-G28/+
Other
Additional Comments
Genetic Interactions
Statement
Reference
The progeny from dpphr27/+ fathers exhibit lethality when mothers are either Med15/+, Df(2R)Pcl11B/+, lolalk02512/+ or lolal1122/+, exhibit near lethality when mothers are either lolalG9603/+ or Df(2R)12/+, and exhibit semi-lethality when mothers are either Mad12/+ or lolal1722/+, as compared to controls; the lethality of the progeny from dpphr27/+ fathers and lolal1122/+ mothers is partially rescued by the maternal expression of either lolalScer\UAS.P\T.cQa, lolalScer\UAS.P\T.T:Ivir\HA1, tkvQ253D.Scer\UAS.P\T, saxQ263D.Scer\UAS.cDa, MedScer\UAS.P\T.cSa or MedK738R.Scer\UAS.P\T, either under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 or, to a lower extent, in the absence of the Gal4 driver.
Double heterozygous dpphr27; fd68A44 mutants exhibit almost 80% viability. Double heterozygous dpphr27; fd68A16 mutants exhibit almost 80% viability.
dpphr27/+ ; Ack86/Ack86 embryos show dorsal closure defects at a relatively low frequency, but which is significantly higher than the frequency seen in either dpphr27/+ or Ack86/Ack86 single mutant embryos.
100% of the expected cype03771/dpphr27 adult progeny are recovered from a cross of dpphr27/+ females to cype03771/+ males. Only 4% of the expected cype03771/dpphr27 adult progeny are recovered from a cross of cype03771/+ females to dpphr27/+ males. The lethality is embryonic and the embryos are weakly to moderately ventralised.
The wing phenotype of dppd5/dpphr27 flies is strongly enhanced by two copies of p38bDN.Scer\UAS expressed under the control of one copy of Scer\GAL432B; veins L4 and L5 are partially fused and the distance between veins L2 and L3 is reduced.
Med1 shows a dominant maternal effect interaction with dpp; when Med1/+ females are crossed to dpphr27/+ males, all progeny carrying dpphr27 die. This lethality is rescued by MedUbi-p63E.PD also partly by MadUbi-p63E.T:Hsap\MYC. Mad12 shows a dominant maternal effect interaction with dpp; when Mad12/+ females are crossed to dpphr27/+ males, all progeny carrying dpphr27 die. This lethality is rescued by MadUbi-p63E.T:Hsap\MYC also partly by MedUbi-p63E.PD.
When in combination with vri mutations, vrik05901 lethal revertant lines or Df(2L)tkv2 lethality is greatly increased and embryos are more strongly ventralised. Escaper vri3 dpphr27 flies exhibit wings with extra vein material between L2 and L3, atrophic wings and a split thorax.
Shows a dominant lethal interaction with sax1 and sax2 : flies transheterozygous for dpphr27 or dpphr93 and sax1 or sax2 fail to eclose.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Allele class: hin-r
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments
Based on considerations of degree of dominant lethality, dpp alleles can be placed in an allelic series. Progressing from weakest to most severe the series is: dppe87 < dppe90 < dpphr56 < dpphr4 < dpphr92 < dpphr27 < dpphr93 < dppH94 < dppH95 < dppH96 = dppH91 = dppH46.
dpphr56 shows interallelic complementation at 18oC with dpphr27.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
References (37)