FlyBase Allele Report: Dmel\dpp[hr27]

dpp^hr27/dpp^hr56 males that have been raised at 18^oC and then shifted to the restrictive temperature of 29^oC upon eclosion do not have any overt morphological abnormalities in the testes after 7 days at 29^oC and the testes contain germ cells in all stages and in quantities indistinguishable from that of controls.

(Shivdasani and Ingham, 2003)

Approximately 50% of eggs laid by heterozygous females have shortened and abnormally shaped respiratory appendages.

(Dequier et al., 2001)

dpp^d6/dpp^hr27 flies show partial fusions of the L2 and L3 and the L4 and L5 wing veins. The wing is compressed along the A/P axis compared to wild type.

(Yu et al., 2000)

The distance between veins L4 and L5 is reduced in dpp^d5/dpp^hr27 flies.

(Adachi-Yamada et al., 1999)

Embryos are ventralised.

(George and Terracol, 1997)

Embryos show cuticular holes, disorganization of the head skeleton, internalization of the seventh and eighth abdominal segments and the filzkorper and a thoracic constriction. The first and second ventral denticle belts are expanded, particularly at the tips. The filzkorper are fused into a single globular mass, reflecting the loss of dorsal fates that keeps the two anlage distinct in wild type. A significant percentage of surviving transheterozygotes between dpp^hr56 and dpp^hr4 or dpp^hr27 have a split notum.

(Wharton et al., 1996)

Survival of heterozygotes is reduced if also heterozygous for certain alleles of tld.

(Finelli et al., 1994)

dpp^hr27/+ females that are also sax¹/+, sax²/+ or tkv⁷/+ (derived from dpp^hr27/+ males) are generally inviable.

(Nellen et al., 1994)

The presence of tkv⁷ in the mother causes dpp^hr27 to behave as a dominant lethal in the zygote.

(Penton et al., 1994)

Embryos display a moderately ventralised phenotype and the mutation has an haploinsufficient effect, a small fraction of heterozygous zygotes die before reaching adulthood. Maternally contributed tkv alleles cause lethality.

(Terracol and Lengyel, 1994)

Clonal analysis in the developing eye (using the FLP/FRT system) revealed nonautonomous phenotypes with large clones showing posterior-lateral eye parts missing.

(Heberlein et al., 1993)

Dominant lethality less than 50%. Homozygous and transheterozygous embryos were examined with respect to 25 cuticular markers, results demonstrate a graded requirement for dpp along the dorso-ventral axis.

(Wharton et al., 1993)

Moderate ventralised phenotype. Rings of ventral denticle belts differentiate around the entire dorsoventral axis, almost no dorsal hairs are seen and the antennal and maxillary sense organs are missing. Defective movements of the germ band: due to loss of the amnioserosa and because the dorsalmost cells have acquired the lateral fate of the dorsal ectoderm. Dorsal cell fates are deleted and ventrolateral mitotic domains are expanded.

(Arora and Nusslein-Volhard, 1992)

Heterozygotes with class I dpp alleles have a wild type wing phenotype. Heterozygotes with class II allele dpp^d5 exhibit wings with a deletion of the anterior crossvein. Heterozygotes with class II allele dpp^d28 exhibit held out wings that are missing longitudinal and cross veins, deletion of tarsal claws, male terminalia and female analia. Heterozygotes with class III dpp alleles display reduced held out wings that are missing longitudinal and cross veins, capitella-less halteres, reduced eyes, legs missing claws and missing or rotated male terminalia or female analia. Heterozygotes with class IV dpp alleles display the same phenotype as class III heterozygotes with duplication of scutellar bristles, medial cleft of the dorsal notum, duplication of sex combs and partial duplication of the third antennal segment. Heterozygotes with class V dpp alleles rarely eclose, the surviors exhibit the wing, haltere, eye and terminalia defects. At 18^oC the heterozygotes have less severe phenotypes.

(Spencer et al., 1982)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

dpp^hr27 has visible | recessive phenotype, enhanceable by Scer\GAL4^32B/p38b^DN.UAS

(Adachi-Yamada et al., 1999)

Suppressed by

Statement

Reference

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by lolal^UASp.cQa/Scer\GAL4^VP16.nos.UTR

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by lolal^UASp.cQa

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by Scer\GAL4^VP16.nos.UTR/Med^K738R.UASp

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by Med^K738R.UASp

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by Scer\GAL4^VP16.nos.UTR/lolal^UASp.Tag:HA

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by lolal^UASp.Tag:HA

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by tkv^Q253D.UASp/Scer\GAL4^VP16.nos.UTR

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by tkv^Q253D.UASp

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by sax^{Q263D.UAS.cDa}/Scer\GAL4^VP16.nos.UTR

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by sax^{Q263D.UAS.cDa}

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by Scer\GAL4^VP16.nos.UTR/Med^UASp.cSa

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype, suppressible | partially by Med^UASp.cSa

(Quijano et al., 2016)

Other

Statement

Reference

Mad¹²/Mad[+], dpp^hr27 has partially lethal - majority die | maternal effect phenotype

(Quijano et al., 2016)

Med[+]/Med¹⁵, dpp^hr27 has lethal | maternal effect phenotype

(Quijano et al., 2016)

Df(2R)Pcl11B/+, dpp^hr27 has lethal | maternal effect phenotype

(Quijano et al., 2016)

dpp^hr27, lolal^k02512/lolal[+] has lethal | maternal effect phenotype

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹¹²² has lethal | maternal effect phenotype

(Quijano et al., 2016)

dpp^hr27, lolal[+]/lolal¹⁷²² has partially lethal - majority die | maternal effect phenotype

(Quijano et al., 2016)

dpp^hr27, lolal^G9603/lolal[+] has partially lethal - majority die | maternal effect phenotype

(Quijano et al., 2016)

Df(2R)12/+, dpp^hr27 has partially lethal - majority die | maternal effect phenotype

(Quijano et al., 2016)

dpp^hr27/dpp[+], sax² has lethal | dominant | maternal effect phenotype

(Twombly et al., 2009)

dpp^hr27/dpp[+], sax¹ has lethal | dominant | maternal effect phenotype

(Twombly et al., 2009)

dpp^hr27/dpp[+], FoxK⁴⁴ has increased mortality during development phenotype

(Casas-Tinto et al., 2008)

dpp^hr27/dpp[+], FoxK¹⁶ has increased mortality during development phenotype

(Casas-Tinto et al., 2008)

dpp^hr27, fd68A[+]/FoxK⁴⁴ has increased mortality during development phenotype

(Casas-Tinto et al., 2008)

Mad¹²/Mad[+], dpp^hr27 has lethal | dominant phenotype

(Das et al., 1998)

Mad¹², dpp^hr27/dpp[+] has lethal | dominant | maternal effect phenotype

(Das et al., 1998)

Med¹, dpp^hr27/dpp[+] has lethal | dominant | maternal effect phenotype

(Das et al., 1998)

Med[+]/Med¹, dpp^hr27 has lethal | dominant phenotype

(Das et al., 1998)

dpp^hr27, sax[+]/sax¹ has lethal | dominant phenotype

(Xie et al., 1994)

dpp^hr27, sax[+]/sax² has lethal | dominant phenotype

(Xie et al., 1994)

dpp^hr27/dpp[+], sax² has lethal | dominant phenotype

(Xie et al., 1994)

dpp^hr27/dpp[+], sax¹ has lethal | dominant phenotype

(Xie et al., 1994)

Phenotype Manifest In

Enhanced by

Statement

Reference

dpp^hr27 has embryonic epidermis phenotype, enhanceable by cype[+]/cype⁰³⁷⁷¹/cype⁰³⁷⁷¹

(Szuplewski and Terracol, 2001)

dpp^hr27 has embryonic/first instar larval cuticle phenotype, enhanceable by cype[+]/cype⁰³⁷⁷¹/cype⁰³⁷⁷¹

(Szuplewski and Terracol, 2001)

dpp^hr27 has wing phenotype, enhanceable by Scer\GAL4^32B/p38b^DN.UAS

(Adachi-Yamada et al., 1999)

dpp^hr27 has phenotype, enhanceable by Df(3L)66C-G28/+

(Nicholls and Gelbart, 1998)

dpp^hr27 has phenotype, enhanceable by vri^5R7.2

(George and Terracol, 1997)

dpp^hr27 has phenotype, enhanceable by vri^5R8.4

(George and Terracol, 1997)

dpp^hr27 has phenotype, enhanceable by vri^k03801

(George and Terracol, 1997)

dpp^hr27 has phenotype, enhanceable by vri^k05901

(George and Terracol, 1997)

dpp^hr27 has phenotype, enhanceable by vri^k09602

(George and Terracol, 1997)

dpp^hr27 has phenotype, enhanceable by vri^k09713

(George and Terracol, 1997)

dpp^hr27 has phenotype, enhanceable by vri^k11805

(George and Terracol, 1997)

dpp^hr27 has phenotype, enhanceable by vri^5R4.11

(George and Terracol, 1997)

dpp^hr27 has phenotype, enhanceable by vri^5R5.24

(George and Terracol, 1997)

dpp^hr27 has phenotype, enhanceable by vri^5R5.7

(George and Terracol, 1997)

dpp^hr27 has phenotype, enhanceable by tld¹⁴

(Raftery et al., 1995)

NOT Enhanced by

Statement

Reference

dpp^Sal20, dpp^hr27 has phenotype, non-enhanceable by Df(3L)66C-G28/+

(Nicholls and Gelbart, 1998)

Other

Statement

Reference

Ack⁸⁶, dpp^hr27/dpp[+] has embryo | dorsal closure stage phenotype

(Zahedi et al., 2008)

Scer\GAL4^32B, dpp^hr27, p38b^DN.UAS has wing vein phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^hr27, p38b^DN.UAS has wing vein L4 phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^hr27, p38b^DN.UAS has wing vein L5 phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^d5/dpp^hr27, p38b^DN.UAS has wing vein phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^d5/dpp^hr27, p38b^DN.UAS has wing vein L4 phenotype

(Adachi-Yamada et al., 1999)

Scer\GAL4^32B, dpp^d5/dpp^hr27, p38b^DN.UAS has wing vein L5 phenotype

(Adachi-Yamada et al., 1999)

dpp^hr27, vri³ has wing vein | ectopic phenotype

(George and Terracol, 1997)

dpp^hr27, vri³ has wing phenotype

(George and Terracol, 1997)

dpp^hr27, vri³ has wing vein phenotype

(George and Terracol, 1997)

dpp^hr27, vri³ has thorax phenotype

(George and Terracol, 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The progeny from dpp^hr27/+ fathers exhibit lethality when mothers are either Med¹⁵/+, Df(2R)Pcl11B/+, lolal^k02512/+ or lolal¹¹²²/+, exhibit near lethality when mothers are either lolal^G9603/+ or Df(2R)12/+, and exhibit semi-lethality when mothers are either Mad¹²/+ or lolal¹⁷²²/+, as compared to controls; the lethality of the progeny from dpp^hr27/+ fathers and lolal¹¹²²/+ mothers is partially rescued by the maternal expression of either lolal^{Scer\UAS.P\T.cQa}, lolal^{Scer\UAS.P\T.T:Ivir\HA1}, tkv^{Q253D.Scer\UAS.P\T}, sax^{Q263D.Scer\UAS.cDa}, Med^{Scer\UAS.P\T.cSa} or Med^{K738R.Scer\UAS.P\T}, either under the control of Scer\GAL4^{nos.UTR.T:Hsim\VP16} or, to a lower extent, in the absence of the Gal4 driver.

(Quijano et al., 2016)

Double heterozygous dpp^hr27; fd68A⁴⁴ mutants exhibit almost 80% viability.

Double heterozygous dpp^hr27; fd68A¹⁶ mutants exhibit almost 80% viability.

(Casas-Tinto et al., 2008)

dpp^hr27/+ ; Ack⁸⁶/Ack⁸⁶ embryos show dorsal closure defects at a relatively low frequency, but which is significantly higher than the frequency seen in either dpp^hr27/+ or Ack⁸⁶/Ack⁸⁶ single mutant embryos.

(Zahedi et al., 2008)

100% of the expected cype⁰³⁷⁷¹/dpp^hr27 adult progeny are recovered from a cross of dpp^hr27/+ females to cype⁰³⁷⁷¹/+ males. Only 4% of the expected cype⁰³⁷⁷¹/dpp^hr27 adult progeny are recovered from a cross of cype⁰³⁷⁷¹/+ females to dpp^hr27/+ males. The lethality is embryonic and the embryos are weakly to moderately ventralised.

(Szuplewski and Terracol, 2001)

The wing phenotype of dpp^d5/dpp^hr27 flies is strongly enhanced by two copies of p38b^DN.Scer\UAS expressed under the control of one copy of Scer\GAL4^32B; veins L4 and L5 are partially fused and the distance between veins L2 and L3 is reduced.

(Adachi-Yamada et al., 1999)

Med¹ shows a dominant maternal effect interaction with dpp; when Med¹/+ females are crossed to dpp^hr27/+ males, all progeny carrying dpp^hr27 die. This lethality is rescued by Med^Ubi-p63E.PD also partly by Mad^{Ubi-p63E.T:Hsap\MYC}. Mad¹² shows a dominant maternal effect interaction with dpp; when Mad¹²/+ females are crossed to dpp^hr27/+ males, all progeny carrying dpp^hr27 die. This lethality is rescued by Mad^{Ubi-p63E.T:Hsap\MYC} also partly by Med^Ubi-p63E.PD.

(Das et al., 1998)

When in combination with vri mutations, vri^k05901 lethal revertant lines or Df(2L)tkv2 lethality is greatly increased and embryos are more strongly ventralised. Escaper vri³ dpp^hr27 flies exhibit wings with extra vein material between L2 and L3, atrophic wings and a split thorax.

(George and Terracol, 1997)

Shows a dominant lethal interaction with sax¹ and sax² : flies transheterozygous for dpp^hr27 or dpp^hr93 and sax¹ or sax² fail to eclose.

(Xie et al., 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Allele class: hin-r

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

58784

dpp^hr27 cn¹ bw¹/CyO, P{dpp-P23}RP1

Notes on Origin

Discoverer

Comments

Based on considerations of degree of dominant lethality, dpp alleles can be placed in an allelic series. Progressing from weakest to most severe the series is: dpp^e87 < dpp^e90 < dpp^hr56 < dpp^hr4 < dpp^hr92 < dpp^hr27 < dpp^hr93 < dpp^H94 < dpp^H95 < dpp^H96 = dpp^H91 = dpp^H46.

(Wharton et al., 1993)

dpp^hr56 shows interallelic complementation at 18^oC with dpp^hr27.

(Wharton et al., 1996)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (7)

Reported As

Symbol Synonym

dpp²⁷

(Iwaki and Lengyel, 2002, Harbecke and Lengyel, 1995, Spencer et al., 1982)

dpp^Hr27

(Casas-Tinto et al., 2008)

dpp^hin-r27

(Ferguson and Anderson, 1992, Irish and Gelbart, 1987)

dpp^hin

(Carneiro et al., 2006)

dpp^hr27

(Tu et al., 2020, Quijano et al., 2016, Stinchfield et al., 2012, Twombly et al., 2009, Miles et al., 2008, Zahedi et al., 2008, Bangi and Wharton, 2006)

dpp^hr2

(Twombly et al., 2009)

hin-r27

(Arora and Nusslein-Volhard, 1992)

Name Synonyms

Secondary FlyBase IDs

References (38)

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