G2457739A
G2133A
E316K | dpp-PA; E316K | dpp-PB; E316K | dpp-PC; E316K | dpp-PE
E316K
Polytene chromosomes normal.
Amino acid replacement: E316K.
Mutation falls in the N-terminal pro region of the dpp protein.
Nucleotide substitution: G2133A.
adult thorax & adult epidermis
denticle belt & thorax
wing vein L2 (with dppd6)
wing vein L4 (with dppd6)
dpphr27/dpphr56 males that have been raised at 18oC and then shifted to the restrictive temperature of 29oC upon eclosion do not have any overt morphological abnormalities in the testes after 7 days at 29oC and the testes contain germ cells in all stages and in quantities indistinguishable from that of controls.
Approximately 50% of eggs laid by heterozygous females have shortened and abnormally shaped respiratory appendages.
Embryos are ventralised.
Embryos show cuticular holes, disorganization of the head skeleton, internalization of the seventh and eighth abdominal segments and the filzkorper and a thoracic constriction. The first and second ventral denticle belts are expanded, particularly at the tips. The filzkorper are fused into a single globular mass, reflecting the loss of dorsal fates that keeps the two anlage distinct in wild type. A significant percentage of surviving transheterozygotes between dpphr56 and dpphr4 or dpphr27 have a split notum.
Survival of heterozygotes is reduced if also heterozygous for certain alleles of tld.
Embryos display a moderately ventralised phenotype and the mutation has an haploinsufficient effect, a small fraction of heterozygous zygotes die before reaching adulthood. Maternally contributed tkv alleles cause lethality.
Clonal analysis in the developing eye (using the FLP/FRT system) revealed nonautonomous phenotypes with large clones showing posterior-lateral eye parts missing.
Dominant lethality less than 50%. Homozygous and transheterozygous embryos were examined with respect to 25 cuticular markers, results demonstrate a graded requirement for dpp along the dorso-ventral axis.
Moderate ventralised phenotype. Rings of ventral denticle belts differentiate around the entire dorsoventral axis, almost no dorsal hairs are seen and the antennal and maxillary sense organs are missing. Defective movements of the germ band: due to loss of the amnioserosa and because the dorsalmost cells have acquired the lateral fate of the dorsal ectoderm. Dorsal cell fates are deleted and ventrolateral mitotic domains are expanded.
Heterozygotes with class I dpp alleles have a wild type wing phenotype. Heterozygotes with class II allele dppd5 exhibit wings with a deletion of the anterior crossvein. Heterozygotes with class II allele dppd28 exhibit held out wings that are missing longitudinal and cross veins, deletion of tarsal claws, male terminalia and female analia. Heterozygotes with class III dpp alleles display reduced held out wings that are missing longitudinal and cross veins, capitella-less halteres, reduced eyes, legs missing claws and missing or rotated male terminalia or female analia. Heterozygotes with class IV dpp alleles display the same phenotype as class III heterozygotes with duplication of scutellar bristles, medial cleft of the dorsal notum, duplication of sex combs and partial duplication of the third antennal segment. Heterozygotes with class V dpp alleles rarely eclose, the surviors exhibit the wing, haltere, eye and terminalia defects. At 18oC the heterozygotes have less severe phenotypes.
dpphr27 has visible | recessive phenotype, enhanceable by Scer\GAL432B/p38bDN.UAS
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by lolalUASp.cQa/Scer\GAL4VP16.nos.UTR
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by lolalUASp.cQa
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by Scer\GAL4VP16.nos.UTR/MedK738R.UASp
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by MedK738R.UASp
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by Scer\GAL4VP16.nos.UTR/lolalUASp.Tag:HA
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by lolalUASp.Tag:HA
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by tkvQ253D.UASp/Scer\GAL4VP16.nos.UTR
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by tkvQ253D.UASp
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by saxQ263D.UAS.cDa/Scer\GAL4VP16.nos.UTR
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by saxQ263D.UAS.cDa
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by Scer\GAL4VP16.nos.UTR/MedUASp.cSa
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype, suppressible | partially by MedUASp.cSa
Mad12/Mad[+], dpphr27 has partially lethal - majority die | maternal effect phenotype
Med[+]/Med15, dpphr27 has lethal | maternal effect phenotype
Df(2R)Pcl11B/+, dpphr27 has lethal | maternal effect phenotype
dpphr27, lolalk02512/lolal[+] has lethal | maternal effect phenotype
dpphr27, lolal[+]/lolal1122 has lethal | maternal effect phenotype
dpphr27, lolal[+]/lolal1722 has partially lethal - majority die | maternal effect phenotype
dpphr27, lolalG9603/lolal[+] has partially lethal - majority die | maternal effect phenotype
Df(2R)12/+, dpphr27 has partially lethal - majority die | maternal effect phenotype
dpphr27/dpp[+], sax2 has lethal | dominant | maternal effect phenotype
dpphr27/dpp[+], sax1 has lethal | dominant | maternal effect phenotype
dpphr27/dpp[+], FoxK44 has increased mortality during development phenotype
dpphr27/dpp[+], FoxK16 has increased mortality during development phenotype
dpphr27, fd68A[+]/FoxK44 has increased mortality during development phenotype
Mad12, dpphr27/dpp[+] has lethal | dominant | maternal effect phenotype
Med1, dpphr27/dpp[+] has lethal | dominant | maternal effect phenotype
dpphr27 has embryonic epidermis phenotype, enhanceable by cype[+]/cype03771/cype03771
dpphr27 has embryonic/first instar larval cuticle phenotype, enhanceable by cype[+]/cype03771/cype03771
dpphr27 has wing phenotype, enhanceable by Scer\GAL432B/p38bDN.UAS
dpphr27 has phenotype, enhanceable by Df(3L)66C-G28/+
dppSal20, dpphr27 has phenotype, non-enhanceable by Df(3L)66C-G28/+
Ack86, dpphr27/dpp[+] has embryo | dorsal closure stage phenotype
Scer\GAL432B, dpphr27, p38bDN.UAS has wing vein phenotype
Scer\GAL432B, dpphr27, p38bDN.UAS has wing vein L4 phenotype
Scer\GAL432B, dpphr27, p38bDN.UAS has wing vein L5 phenotype
Scer\GAL432B, dppd5/dpphr27, p38bDN.UAS has wing vein phenotype
Scer\GAL432B, dppd5/dpphr27, p38bDN.UAS has wing vein L4 phenotype
Scer\GAL432B, dppd5/dpphr27, p38bDN.UAS has wing vein L5 phenotype
The progeny from dpphr27/+ fathers exhibit lethality when mothers are either Med15/+, Df(2R)Pcl11B/+, lolalk02512/+ or lolal1122/+, exhibit near lethality when mothers are either lolalG9603/+ or Df(2R)12/+, and exhibit semi-lethality when mothers are either Mad12/+ or lolal1722/+, as compared to controls; the lethality of the progeny from dpphr27/+ fathers and lolal1122/+ mothers is partially rescued by the maternal expression of either lolalScer\UAS.P\T.cQa, lolalScer\UAS.P\T.T:Ivir\HA1, tkvQ253D.Scer\UAS.P\T, saxQ263D.Scer\UAS.cDa, MedScer\UAS.P\T.cSa or MedK738R.Scer\UAS.P\T, either under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 or, to a lower extent, in the absence of the Gal4 driver.
100% of the expected cype03771/dpphr27 adult progeny are recovered from a cross of dpphr27/+ females to cype03771/+ males. Only 4% of the expected cype03771/dpphr27 adult progeny are recovered from a cross of cype03771/+ females to dpphr27/+ males. The lethality is embryonic and the embryos are weakly to moderately ventralised.
The wing phenotype of dppd5/dpphr27 flies is strongly enhanced by two copies of p38bDN.Scer\UAS expressed under the control of one copy of Scer\GAL432B; veins L4 and L5 are partially fused and the distance between veins L2 and L3 is reduced.
Med1 shows a dominant maternal effect interaction with dpp; when Med1/+ females are crossed to dpphr27/+ males, all progeny carrying dpphr27 die. This lethality is rescued by MedUbi-p63E.PD also partly by MadUbi-p63E.T:Hsap\MYC. Mad12 shows a dominant maternal effect interaction with dpp; when Mad12/+ females are crossed to dpphr27/+ males, all progeny carrying dpphr27 die. This lethality is rescued by MadUbi-p63E.T:Hsap\MYC also partly by MedUbi-p63E.PD.
When in combination with vri mutations, vrik05901 lethal revertant lines or Df(2L)tkv2 lethality is greatly increased and embryos are more strongly ventralised. Escaper vri3 dpphr27 flies exhibit wings with extra vein material between L2 and L3, atrophic wings and a split thorax.
Allele class: hin-r
Based on considerations of degree of dominant lethality, dpp alleles can be placed in an allelic series. Progressing from weakest to most severe the series is: dppe87 < dppe90 < dpphr56 < dpphr4 < dpphr92 < dpphr27 < dpphr93 < dppH94 < dppH95 < dppH96 = dppH91 = dppH46.
dpphr56 shows interallelic complementation at 18oC with dpphr27.