Allele Dmel\Dr^Mio

General Information
Symbol	Dmel\Dr^Mio	Species	D. melanogaster
Name	Microphthalmia	FlyBase ID	FBal0003110
Feature type	allele	Created / Updated	2006-05-15/2006-05-15
Associated gene	Dmel\Dr

Allele class	gain of function
Mutagen	nitrogen mustard
Nature of the Allele
Allele class	gain of function (Tearle et al., 1994, Mozer and Easwarachandran, 1999)
Mutagen	nitrogen mustard
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference 3S18 insertion 27kb upstream of the start of the Dr transcript. (Mozer, 2001)
Assay mode
Caused by insertion	3S18{}Dr^Mio (Mozer, 2001, Mozer, 1995)
Carried on aberration	TM6B-TD (FlyBase Curators, 2006-, Bloomington Drosophila Stock Center, 2001.8.28)
Cytology
Phenotypic Data
Phenotypic Class
	viable (with Df(3R)KE) (Mozer, 2001) viable (with Dr^ltt-EMS) (Mozer, 2001) viable \| reduced (Tearle et al., 1994) lethal \| recessive (Renfranz and Benzer, 1989) lethal \| embryonic stage \| recessive (Tearle et al., 1994) visible \| dominant (Tearle et al., 1994, Mozer, 2001) locomotor behavior defective (Tearle et al., 1994) uncoordinated (with Dr¹) (Tearle et al., 1994) uncoordinated (with Dr^1E1) (Tearle et al., 1994) uncoordinated (with Dr^1E2) (Tearle et al., 1994) uncoordinated (with Dr^1E3) (Tearle et al., 1994) uncoordinated (with Dr^ME10) (Tearle et al., 1994) uncoordinated (with Dr^ME11) (Tearle et al., 1994) uncoordinated (with Dr^ME4) (Tearle et al., 1994) uncoordinated (with Dr^ME8) (Tearle et al., 1994) uncoordinated (with Dr^ME9) (Tearle et al., 1994) uncoordinated (with Dr^RXT1) (Tearle et al., 1994) uncoordinated (with Dr^RXT10) (Tearle et al., 1994) uncoordinated (with Dr^RXT12) (Tearle et al., 1994) uncoordinated (with Dr^RXT15) (Tearle et al., 1994) cell death increase \| dominant (Mozer, 2001)
Phenotype Manifest In
	macrochaeta (Tearle et al., 1994) eye (Tearle et al., 1994, Mozer, 2001) ommatidium (Mozer, 2001) five-cell ommatidial precursor (Mozer, 2001)
Detailed Description
	Statement Reference homozygous lethal Adult eyes are very reduced and rough with about 30 facets. Some rhabdomeres within ommatidia are fused. Eye disc size is reduced. (Renfranz and Benzer, 1989) The progression of the morphogenetic furrow in the developing eye disc arrests. The eye disc appears normal at the time of arrest. dpp expression is abolished (as assayed with a dpp-lacZ fusion gene). (Heberlein et al., 1993) Severe disruption of eye development. Heterozygotes with Dr¹ are semi-lethal, 10-30% adults survive to eclosion, surviving adults exhibit reduced eye, deranged bristle pattern on the thorax, abdomen, legs and wing margins and locomotor defects so the flies could hardly move. A similar phenotype is seen when heterozygous with Dr¹ revertant alleles and stg alleles. Heterozygotes with Wedge¹ only exhibit the reduced eye phenotype. Interact in trans with lesions in stg causing loss and derangement of bristles and loss of neuromuscular coordination. (Tearle et al., 1994) Mutants display locomotor activity rhythms with circadian periods, though with reduced penetrance (small sample size). Average period length of the locomotor activity is rather short. (Vosshall and Young, 1995) Heterozygotes have severely reduced eyes, containing less than 30 ommatidia. The shape of the mutant eye resembles an inverted drop. No patterning defects are seen in the retina. Five-cell preclusters are not seen in the heterozygous eye disc at the time when multiple rows of developing ommatidia are seen in wild-type discs, and instead a single row of mature ommatidial clusters is seen. Massive cell death associated with absence of furrow progression is seen. (Mozer, 2001)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Dr^Mio has phenotype, suppressible \| partially by amos^Roi-1 (Heberlein et al., 1993)
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 15 )
Bloomington	406 Dr^Mio/TMS, P{Δ2-3}99B 491 Dr^Mio/TM6, Ubx⁺ 3644 TM3, Sb¹/TM6B, Tb¹ Dr^Mio 3218 TM3, P{ftz-lacZ.ry⁺}TM3, Sb¹ ry^/Dr^Mio 1255 TM6B, Dr^Mio Tb⁺/Ubx^Cbx-1 Ubx¹ gl³ 7 P{hsFLP}1, y¹ w¹¹¹⁸; Dr^Mio/TM3, ry^ Sb¹ 1289 C(1)M4, y²; TM6B, Tb¹ Dr^Mio/In(3R)Dl^B, Dl^B 6663 w¹¹¹⁸; Dr^Mio/TM3, P{GAL4-twi.G}2.3, P{UAS-2xEGFP}AH2.3, Sb¹ Ser¹ 1189 emc^D st¹ in¹ kni^ri-1 p^p Dr^Mio/TM6C, ca¹
Kyoto	107153 TM3, Sb¹/TM6B, Tb¹ Dr^Mio 106888 T(2;3)CyO-TM2, CyO, l(2)DTS513¹: TM2/Dr^Mio 105671 P{hsFLP}1, y¹ w¹¹¹⁸; Dr^Mio/TM3, ry^* Sb¹
	More stocks available...
Notes on Origin
Discoverer	Sobels, 22nd Oct. 1957. Sobels.
	Associated with: stg^Dr-Mio. (Mozer and Easwarachandran, 1999)
Synonyms & Secondary IDs ( 4 )
Reported As
Symbol Synonym	Dr^Mio Mio
Name Synonym	Microphthalmia Miopthalmia (Vosshall and Young, 1995)
Secondary FlyBase IDs

References ( 15 )
Research paper	Mozer, 2001, Dev. Biol. 233(2): 380--393 Dominant Drop mutants are gain-of-function alleles of the muscle segment homeobox gene (msh) whose overexpression leads to the arrest of eye development. [FBrf0136848] Mozer and Easwarachandran, 1999, Dev. Biol. 213(1): 54--69 Pattern formation in the absence of cell proliferation: tissue-specific regulation of cell cycle progression by string (stg) during Drosophila eye development. [FBrf0111437] Vosshall and Young, 1995, Neuron 15(2): 345--360 Circadian rhythms in Drosophila can be driven by period expression in a restricted group of central brain cells. [FBrf0083505] Tearle et al., 1994, Molec. gen. Genet. 244(4): 426--434 The dominant Drop eye mutations of Drosophila melanogaster define two loci implicated in normal eye development. [FBrf0076958] Heberlein et al., 1993, Cell 75(5): 913--926 The TGF homolog dpp and the segment polarity gene hedgehog are required for propagation of a morphogenetic wave in the Drosophila retina. [FBrf0064498] Renfranz and Benzer, 1989, Dev. Biol. 136(2): 411--429 Monoclonal antibody probes discriminate early and late mutant defects in development of the Drosophila retina. [FBrf0049495] Sobels, 1958, D. I. S. 32: 84--86 [New mutants report.] [FBrf0063881]
Review	Bonini and Fortini, 1999, BioEssays 21(12): 991--1003 Surviving Drosophila eye development: integrating cell death with differentiation during formation of a neural structure. [FBrf0122971] Thomas and Wassarman, 1999, Trends Genet. 15(5): 184--190 A fly's eye view of biology. [FBrf0108464]
Personal communication to FlyBase	Bloomington Drosophila Stock Center, 2001.8.28, Balancer Variants. Balancer Variants. [FBrf0138610]
Abstract	Mozer et al., 2001, Europ. Dros. Res. Conf. 17: N23 Dominant Drop (Dr) mutants are gain of function alleles of the muscle segment homeobox gene (msh) whose over-expression leads to the arrest of eye development. [FBrf0138187] Mozer, 1997, Doe, Hall, 1997: 133 The Drop-proximal (Drprox) gene negatively regulates neuronal cell fate determination during eye development. [FBrf0099410] Mozer, 1995, A. Dros. Res. Conf. 36: 215A Cell cycle regulation in visual system development by the Drop gene. [FBrf0079057] Mozer, 1995, Posakony, White, 1995: 120 Dominant small eye mutations in the Drop locus show genetic interactions with string and roughex and identify a novel cell cycle control gene. [FBrf0084206]
FlyBase analysis	FlyBase Curators, 2006-, Balancer summary information. Balancer summary information. [FBrf0189689]

Allele Dmel\DrMio

Allele Dmel\Dr^Mio