embryonic leading edge cell & actin filament
embryonic leading edge cell & filopodium
embryonic leading edge cell & microtubule
macrochaeta & wing
wing & macrochaeta
wing & triple row | somatic clone
Double-heterozygous flies of the genotype dsh/+ together with either kmr1/+ or kmr2/+ display misoriented hair patterns on the wing, thorax and eyes, while single heterozygotes exhibit phenotypes similar to wild-type controls.
The segment polarity phenotype and the lack of naked cuticle between denticle belts, resulting in a lawn of denticles on the ventral epidermis, characteristic for maternally and zygotically mutant dsh3 embryos is not modified by Scer\GAL4arm.PU-driven expression of any of the following: sggScer\UAS.T:Myr-Src64B,T:Ivir\HA1, sggKK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1, sggScer\UAS.T:Ivir\HA1 or sggKK-MI.Scer\UAS.T:Ivir\HA1.
dsh3 maternal/l(2)gl4 zygotic double mutants display a cuticle which resembles the l(2)gl4 maternal mutant phenotype. Patches of denticles are observed which lack proper alignment, and the ventral epithelium is continuous and formed. Denticle precursors are not properly oriented toward the posterior of cells and denticle placement appears to be random within each cell.
dsh3 fz21/fz21 clones in the pupal wing (32 hours after puparium formation) cause neighbouring cells to point their trichomes towards the clone, as occurs with fz21/fz21 single mutant clones in the pupal wing.
dsh3 Vangstbm-6/Vangstbm-6 clones in the pupal wing (32 hours after puparium formation) cause neighbouring cells to point their trichomes away from the clone, as occurs with Vangstbm-6/Vangstbm-6 single mutant clones in the pupal wing.
Expression of dcoScer\UAS.cKa under the control of Scer\GAL4hs.2sev in a dsh3/+ background completely suppresses the planar cell polarity and photoreceptor number phenotypes seen when dcoScer\UAS.cKa is expressed in a wild-type background.
The rapid loss of smo3 homozygous somatic clone cells from the somatic stem cell population of the germarium is significantly suppressed if the clone cells are also tkvQ199D.Scer\UAS; Scer\GAL4Act5C.PI (Scer\GAL80 method).
Unlike embryos from dsh3 germline clones, embryos from dsh3; sggM11 germ-line clones have no dorsal hole in their cuticles (although their dorsal cuticle is severely puckered), and have a dorso-ventrally polarised and extended leading edge at stage 13.
dshmut6.T:Avic\GFP-EGFP rescues the embryonic lethality of dsh3 animals. The resulting adults have largely wild-type wings and both leg bristles and wing hairs show normal planar polarisation. The rescued adults show a strong ectopic leg joint phenotype.
dsh3 flies carrying dsh1.tAa are viable and have a dsh1 phenotype. Mutant embryos show shortening of the cuticle, fusion of denticle bands and absence the head skeleton and filzkorper. This phenotype is completely rescued by injection of dshcAa or dshΔbPDZ mRNA, partially rescued by injection of dshΔDIX or dshΔEP+ mRNA, and is not rescued by injection of dshΔDEP+, dshDIX, dshbPDZ or dshDEP+ mRNA.