Allele Dmel\dsx⁹

General Information
Symbol	Dmel\dsx⁹	Species	D. melanogaster
Name		FlyBase ID	FBal0003162
Feature type	allele	Associated gene	Dmel\dsx

Allele class
Mutagen
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Deletion beginning at amino acid 65 and extending towards the C terminal of the protein. (Erdman and Burtis, 1993)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference Df(2R)en-B/ix³, dsx[+]/dsx⁹ has viable phenotype (Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹/dsx¹ has viable phenotype (Garrett-Engele et al., 2002) dsx⁹/dsx¹, ix³ has viable phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002)
Phenotype Manifest In
Other
Statement Reference Df(2R)en-B/+, dsx⁹/dsx¹ has abdominal sternite 6 phenotype (Garrett-Engele et al., 2002) Df(2R)en-B/+, dsx⁹/dsx¹ has abdominal tergite 6 \| female phenotype (Garrett-Engele et al., 2002) Df(2R)en-B/+, dsx⁹/dsx¹ has epiproct phenotype (Garrett-Engele et al., 2002) Df(2R)en-B/+, dsx⁹/dsx¹ has gonopod thorn bristle phenotype (Garrett-Engele et al., 2002) Df(2R)en-B/+, dsx⁹/dsx¹ has prothoracic metatarsus \| female phenotype (Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹/dsx¹ has abdominal sternite 6 \| male phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹/dsx¹ has abdominal tergite 6 \| female phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹/dsx¹ has anal plate phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹/dsx¹ has epiproct phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹/dsx¹ has gonopod thorn bristle phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹/dsx¹ has prothoracic metatarsus phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹/dsx¹ has sex comb phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹ has abdominal sternite 6 \| male phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹ has abdominal tergite 6 \| female phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹ has anal plate phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹ has epiproct phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹ has gonopod thorn bristle phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹ has prothoracic metatarsus phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) Df(2R)en-B/ix³, dsx⁹ has sex comb phenotype (Garrett-Engele et al., 2002, Garrett-Engele et al., 2002) dsx⁹/dsx¹, ix³/ix[+] has abdominal sternite 6 phenotype (Garrett-Engele et al., 2002) dsx⁹/dsx¹, ix³/ix[+] has abdominal tergite 6 \| female phenotype (Garrett-Engele et al., 2002) dsx⁹/dsx¹, ix³/ix[+] has epiproct phenotype (Garrett-Engele et al., 2002) dsx⁹/dsx¹, ix³/ix[+] has gonopod thorn bristle phenotype (Garrett-Engele et al., 2002) dsx⁹/dsx¹, ix³/ix[+] has prothoracic metatarsus \| female phenotype (Garrett-Engele et al., 2002) dsx⁹/dsx¹, ix³ has abdominal sternite 6 phenotype (Garrett-Engele et al., 2002) dsx⁹/dsx¹, ix³ has abdominal tergite 6 \| female phenotype (Garrett-Engele et al., 2002) dsx⁹/dsx¹, ix³ has epiproct phenotype (Garrett-Engele et al., 2002) dsx⁹/dsx¹, ix³ has gonopod thorn bristle phenotype (Garrett-Engele et al., 2002) dsx⁹/dsx¹, ix³ has prothoracic metatarsus \| female phenotype (Garrett-Engele et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference ix; dsx transheterozygous progeny from ix³/+; dsx¹/+ females crossed to Df(2R)en-B/+; dsx⁹/+ males show little or no masculinisation of females, or feminisation of males, as judged by: Mean number of vaginal teeth; percentage of females with fused dorsal-lateral anal plates; percentage width of the 6th tergite that is darkly pigmented; number of bristles in the last transverse row in the foreleg basitarsus (note this row forms the sex comb in males); or number of bristles on the sixth sternite. ix³/Df(2R)en-B; dsx⁹/dsx¹ double mutants show masculinisation of females, as judged by the following criteria: The mean number of vaginal teeth is reduced to 6.45 +/- 2.11. 85 % of females have fused dorsal-lateral anal plates (as wild type; note that even those with fused plates exhibit variable degrees of partial fusion as judged against fusion in wild-type females). The mean percentage width of the 6th tergite that is darkly pigmented is increased to 95% (+/- 0.4%). The number of bristles in the last transverse row in the foreleg basitarsus is increased to: 7.25 +/- 0.54 ( this row forms the sex comb in males). ix³/Df(2R)en-B; dsx⁹/dsx¹ double mutant females do not show significant reductions in the number of bristles on the sixth sternite (20.75 +/- 1.83). ix³/Df(2R)en-B; dsx⁹/dsx¹ double mutants show feminisation of males, as judged by the following criteria: An average of 6.85 (+/- 2.74) ectopic vaginal teeth form. Fusion of dorso-lateral anal plates is seen in 80% of males. The number of bristles in the last transverse row in the foreleg basitarsus is decreased to 7.25 +/-0.54 (this row forms the sex comb in males). The average number of bristles on the sixth sternite increases dramatically from near zero to 18.2 +/- 1.58. ix³/Df(2R)en-B; dsx⁹/dsx¹ double mutant males do not show significant masculinisation of pigmentation of the 6th tergite, which remains 96% (+/-3%) darkly pigmented. ix³/Df(2R)en-B mutant females heterozygous for dsx⁹ or dsx¹ show masculinisation, as judged by the following criteria: Mean number of vaginal teeth is reduced to 9.7 +/- 5.29. 90 % of females have fused dorsal-lateral anal plates (as wild type; note that even those with fused plates exhibit variable degrees of partial fusion as judged against fusion in wild-type females). The mean percentage width of the 6th tergite that is darkly pigmented is increased to 92% (+/-6%). The number of bristles in the last transverse row in the foreleg basitarsus is increased to: 6.88 +/- 0.65 (this row forms the sex comb in males). ix³/Df(2R)en-B mutant females transheterozygous for dsx⁹/+ or dsx¹/+ do not show significant reductions in the number of bristles on the sixth sternite (21.1 +/-1.68) ix³/Df(2R)en-B mutant males heterozygous for dsx⁹ or dsx¹ do not show feminisation, as judged by the following criteria: Mean number of vaginal teeth; percentage of males with fused dorsal-lateral anal plates; percentage width of the 6th tergite that is darkly pigmented; number of bristles in the last transverse row in the foreleg basitarsus (note this row forms the sex comb in males); or number of bristles on the sixth sternite. dsx⁹/dsx¹ mutant females heterozygous for ix³ or Df(2R)en-B show masculinisation, as judged by the following criteria: Mean number of vaginal teeth is reduced to 6.0 +/-3.55. 65 % of females have fused dorsal-lateral anal plates (as wild type; note that even those with fused plates exhibit variable degrees of partial fusion as judged against fusion in wild-type females). The mean percentage width of the 6th tergite that is darkly pigmented is increased to 96% (+/-5%). The number of bristles in the last transverse row in the foreleg basitarsus is increased to: 7.75 +/- 0.78 (this row forms the sex comb in males). The average number of bristles on the sixth sternite is slightly decreased to 19.5 +/- 1.91. dsx⁹/dsx¹ mutant males heterozygous for ix³ or Df(2R)en-B show feminisation, as judged by the following criteria: An average of 6 (+/- 4.19) ectopic vaginal teeth form. Fusion of dorso-lateral anal plates is seen in 70% of males. The number of bristles in the last transverse row in the foreleg basitarsus is decreased to 7.45 +/-0.6 (this row forms the sex comb in males). The average number of bristles on the sixth sternite increases dramatically from near zero to 17.3 +/- 2.06. dsx⁹/dsx¹ mutant males heterozygous for ix³ or Df(2R)en-B do not show significant masculinisation of pigmentation of the 6th tergite, which remains 95% (+/-4%) darkly pigmented. (Garrett-Engele et al., 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer	Garen.

Comments
	No sequence specific DNA binding activity by encoded product in gel mobility shift assays. (Erdman and Burtis, 1993)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	dsx⁹ dsx^127-1 (Erdman and Burtis, 1993) dsx¹²⁷ (Garrett-Engele et al., 2002, )
Name Synonym
Secondary FlyBase IDs

References ( 2 )
Research paper	Garrett-Engele et al., 2002, Development 129(20): 4661--4675 intersex, a gene required for female sexual development in Drosophila, is expressed in both sexes and functions together with doublesex to regulate terminal differentiation. [FBrf0151927] Erdman and Burtis, 1993, EMBO J. 12(2): 527--535 The Drosophila doublesex proteins share a novel zinc finger related DNA binding domain. [FBrf0058162]

Allele Dmel\dsx9

Allele Dmel\dsx⁹