FlyBase Allele Report: Dmel\CkIIβ[And]

General Information

Symbol

Dmel\CkIIβ^And

Species

D. melanogaster

Name

Andante

FlyBase ID

FBal0003276

Feature type

allele

Associated gene

Dmel\CkIIβ

Associated Insertion(s)

Carried in Construct

Also Known As

dy^And

Key Links

Genomic Maps

JBrowse

Allele class

hypomorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

hypomorphic allele - genetic evidence

(Newby et al., 1991)

Mutagen

ethyl methanesulfonate

(Newby and Jackson, 1995, Konopka et al., 1991, Newby et al., 1991)

Progenitor genotype

Cytology

Polytene chromosomes normal

(Newby et al., 1991)

Description

Amino acid replacement: M166I.

(Jauch et al., 2006)

The M166I substitution occurs within an α-helical domain that is shared by all identified CkIIβ isoforms.

(Jauch et al., 2006)

This allele also carries intronic polymorphisms.

(Jauch et al., 2006)

Nucleotide substitution: G3870A. Amino acid replacement: M166I.

(Akten et al., 2003)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:11,794,949..11,794,949 [+]

Nucleotide change:

G11794949A

Reported nucleotide change:

G3870A

Amino acid change:

Reported amino acid change:

M166I

(Akten et al., 2003)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

calyx of adult mushroom body

(Jauch et al., 2006)

calyx of adult mushroom body (with CkIIβ^mbuΔA26-2L)

(Jauch et al., 2006)

egg chorion | maternal effect

(Wong et al., 2011)

Detailed Description

Statement

Reference

Almost 10% of eggs laid by homozygous CkIIβ^And females have ventralised chorion.

(Wong et al., 2011)

CkIIβ^And/Y adults have a longer circadian periodicity of locomotor activity than wild-type controls.

(Akten et al., 2009)

The mushroom body calyx of CkIIβ^And adult fly brains is approximately a quarter of the size of that in wild type flies.

The mushroom body calyx of adult flies transheterozygous for CkIIβ^And and CkIIβ^mbuΔA26-2L is approximately a third of the size of that in wild type flies.

(Jauch et al., 2006)

Mutant hemizygotes have a circadian rhythm of 25.8 hours. CkIIβ^mbuP1/CkIIβ^And transheterozygotes have a circadian rhythm of 24.6 hours.

(Akten et al., 2003)

Shows a circadian long-period phenotype.

(Jackson, 2002.7.26)

Circadian period defects. Gynandromorph mosaics demonstrate there is no correlation between body genotype and circadian rhythmicity. There is correlation, but it is not perfect, between genotype of head cuticle and circadian rhythmicity suggesting circadian function is mediated by cells within the head.

(Newby and Jackson, 1995)

CkIIβ^And combined with per^T exhibits a 1 hour lengthening of the adult periodicities, an additive effect.

(Konopka et al., 1994)

The normal free-running 24 hr periods of the circadian rhythms of eclosion and adult locomotor activity (in constant conditions) are lengthened by 1.5-2 hr/cycle; CkIIβ^And/+ heterozygotes have a period phenotype intermediate between wild-type and mutant homozygotes (Konopka, R. Smith and Orr). CkIIβ^And males are defective in after effects on courtship behavior that are usually induced by prior exposure to mated females or very young males (FBrf0039828). CkIIβ^And lengthens in an additive manner, the periodicities associated with certain other rhythm mutants, i.e., those which by themselves cause shorter- or longer-than-normal locomotor activity periods (viz, per^s, per^L1, per^L2, Clk).

(Hall, 1992)

The period of eclosion rhythm is lengthened by about 1.2 hours in homozygous males and females compared to wild-type. The periods of heterozygotes are only slightly longer than wild-type periods. The period of locomotor rhythm is lengthened compared to wild-type in both heterozygotes and homozygotes. Heterozygotes have locomotor rhythm periods intermediate between that of the homozygotes and wild-type, indicating that this aspect of the phenotype is semidominant. The phase response curves for the circadian eclosion and locomotor activity rhythms, indicating light-induced phase shifts, are also lengthened compared to wild-type. The locomotor activity rhythm is highly temperature compensated, similar to wild-type.

(Konopka et al., 1991)

Circadian periods (eclosion and activity rhythms) are lengthened by 1.5 hrs.

(Newby et al., 1991)

Hemizygous males have a slightly longer than normal song rhythm periodicities.

(Hall, 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

NOT suppressed by

Statement

Reference

CkIIβ^And has abnormal locomotor rhythm phenotype, non-suppressible by S6kII^ign-Δ58-1

(Akten et al., 2009)

Suppressor of

Statement

Reference

CkIIβ^And is a suppressor of abnormal locomotor rhythm phenotype of S6kII^ign-Δ58-1

(Akten et al., 2009)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

The increased periodicity of circadian locomoter acticvity seen in CkIIβ^And/Y adults is not suppressed by S6kII^ign-Δ58-1/Y.

(Akten et al., 2009)

CkIIβ^And lengthens the activity rhythm period of per^s/Y, per^L1/Y, per^L2/Y and per^Clk/Y flies. so^unspecified has no effect on the period of CkIIβ^And flies.

The activity rhythm period of dy^unspecified/CkIIβ^And or m^D/CkIIβ^And flies is indistinguishable from that of CkIIβ^And/+ flies.

(Konopka et al., 1991)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Complements

CkIIβ^mbuΔA6-1

(Akten et al., 2003)

CkIIβ^mbuP1

(Akten et al., 2003)

Fails to complement

CkIIβ^mbuΔA20-1L

(Akten et al., 2003)

CkIIβ^mbuΔA26-2L

(Akten et al., 2003)

Rescued by

CkIIβ^And is rescued by CkIIβ^gDNA

(Akten et al., 2003)

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Konopka, R. Smith and Orr, 1976.

Separable from: dy^Q189stop.

(Jackson, 2002.7.26)

Associated with: dy^Q189stop.

(Konopka et al., 1991)

The 'Andante' mutant chromosome carries two independent EMS-induced mutations, one in dy (dy^Q189stop) and one in CkIIβ (CkIIβ^And). The dy^Q189stop mutation has no effect on rhythmicity, rather the CkIIβ^And allele is responsible for the circadian long-period phenotype of the mutant chromosome.

(Jackson, 2002.7.26)

Alleles previously referred to as "dy[n1]", "dy[n3]" and "dy[n4]" are re-isolates of CkIIβ^And, based on the molecular characterization of polymorphisms in these strains and CkIIβ^And.

(Jackson, 2002.7.26)

Comments

The mushroom body phenotype of CkIIβ^And flies may be separable from the M166I mutation, as a transgenic construct carrying this mutation is able to rescue the mushroom body phenotype of CkIIβ null mutant flies.

(Jauch et al., 2006)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (10)

Reported As

Symbol Synonym

And

(Jackson and Newby, 1993, Konopka et al., 1991, Hall, 1990)

Andante

(Akten et al., 2009)

CkIIβ^And

ck2β^And

(Wong et al., 2011)

dy^And

(Roberts and Jackson, 1997, Newby and Jackson, 1995, Konopka et al., 1994, Newby et al., 1991)

dy^Andante

(Stanewsky, 2003)

dyⁿ¹

(Stanewsky, 2003)

dyⁿ³

(Stanewsky, 2003)

dyⁿ⁴

(Stanewsky, 2003)

Name Synonyms

Andante

(Jackson, 2002.7.26)

DmCK2βAndante

(Jauch et al., 2006)

Secondary FlyBase IDs

FBal0031147
FBal0031149
FBal0031150

References (16)

Report Sections

Open Close