FlyBase Allele Report: Dmel\E(Pc)[1]

General Information

Symbol

Dmel\E(Pc)¹

Species

D. melanogaster

Name

FlyBase ID

FBal0003311

Feature type

allele

Associated gene

Dmel\E(Pc)

Associated Insertion(s)

Carried in Construct

Also Known As

E(Pc)

Key Links

Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Lindsley and Zimm, 1992, Sato et al., 1983)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

exacerbates hematologic cancer

modeled by hop^Tum

(Bailetti et al., 2019)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy | recessive

(Tea and Luo, 2011)

decreased cell number | recessive

(Tea and Luo, 2011)

increased cell number | somatic clone | spermatogenesis

(Feng et al., 2017)

increased cell number | spermatogenesis, with E(Pc)^JF03101, Scer\GAL4^NP1624

(Feng et al., 2017)

lethal (with Df(2R)BSC160)

(Kahsai et al., 2016)

lethal | recessive

(Lindsley and Zimm, 1992, Sato et al., 1983)

neoplasia | spermatogenesis, with E(Pc)^JF03101, Scer\GAL4^NP1624

(Feng et al., 2017)

radiation resistant | dominant

(Holmes et al., 2006)

suppressor of variegation | dominant, with Dp(1;Y)Bar^SCV

(Sinclair et al., 1998)

suppressor of variegation | dominant, with In(1)w^m4

(Sinclair et al., 1998)

suppressor of variegation | dominant, with In(2R)bw^VDe2

(Sinclair et al., 1998)

suppressor of variegation | dominant, with T(2;3)Sb^V

(Sinclair et al., 1998)

Phenotype Manifest In

adult antennal lobe projection neuron DL1 adPN

(Tea and Luo, 2011)

cyst progenitor cell | increased number, with E(Pc)^JF03101, Scer\GAL4^NP1624

(Feng et al., 2017)

cyst progenitor cell | somatic clone

(Feng et al., 2017)

male germline cell | somatic clone | increased number

(Feng et al., 2017)

Detailed Description

Statement

Reference

In mosaic testes, E(Pc)¹ cyst stem cell clones lead to differentiation defects, with a high proportion of cells abnormally co-expressing Zfh-1 (marker of early stage cyst cells) and Eya (marker of later stage cyst cells). Moreover, in a few testes, extra DAPI-bright cells intermingle with Zfh-1-positive cells, suggesting an excess of early germ cells.

The excess early germ cell phenotype of Scer\GAL4^NP1624>E(Pc)^JF03101 is significant;y enhanced by E(Pc)¹ heterozygosity.

(Feng et al., 2017)

In E(Pc)¹ mutants, PBac{GH146-GAL4.B}-marked DL1 olfactory projection neurons mistarget some of their dendrites to the dorsolateral glomerulus DA41 in the anterior of the antennal lobe instead of the DL1 glomerulus in the posterior of the antennal lobe as in wild type. However, arborization of mutant axons is not obviously altered.

PBac{GH146-GAL4.B}-marked anterodorsal and lateral projection neuron neuroblast clones in dom^LL05537 mutants have a very mild reduction in cell number and their dendrites do no t traget to the full set of proper glomeruli.

PBac{GH146-GAL4.B}-marked ventral projection neuron neuroblast clones in dom^LL05537 mutants exhibit incomplete targeting throughout the antennal lobe.

(Tea and Luo, 2011)

Heterozygotes show a significant increase in double strand break (DSB) repair via homologous recombination and a corresponding decrease in DSB repair by nonhomologous end-joining compared to controls in a somatic DSB repair assay, when either w^hd80k17 or w^hd-y+ is used as the recipient allele bearing the target break site.

The genome of E(Pc)¹/+ flies appears to be significantly more stable following exposure to irradiation (seen as induction of fewer bristles with a Minute phenotype) compared to control flies.

Heterozygous wing discs show an approximately twofold reduction in radiation-induced apoptosis compared to controls.

Heterozygotes show an increased survival rate following irradiation compared to control animals.

(Holmes et al., 2006)

Viable in transheterozygous combination with E(Pc)^rv4, E(Pc)^rv9 or E(Pc)^rv10. Dominantly suppresses position effect variegation (PEV) at the w locus caused by In(1)w^m4, PEV at the B locus caused by Dp(1;Y)B^SCV, PEV at the Sb locus caused by T(2;3)Sb^V and PEV at the bw locus caused by In(2R)bw^VDe2.

(Sinclair et al., 1998)

Mutation changes the level of w expression in ph-p^lac+3 flies; eye colour is darker.

(Fauvarque et al., 1995)

The cuticles of embryos lacking both maternal and zygotic E(Pc) product appear wild type.

(Soto et al., 1995)

No homeotic phenotype in embryos or adults. Strong enhancers of Pc, Pcl and pho mutations (FBrf0040741).

(Cheng et al., 1994)

E(Pc)¹ heterozygotes are cuticularly normal as larvae and adults. E(Pc)¹ is a recessive lethal mutation; homozygotes and hemizygotes die as late embryos or larvae, which appear cuticularly normal.

(Lindsley and Zimm, 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

E(Pc)¹ is an enhancer of visible phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

E(Pc)[+]/E(Pc)¹ is an enhancer of visible | dominant | homeotic phenotype of brm[+]/brm², trx^E2

(Gildea et al., 2000)

E(Pc)[+]/E(Pc)¹ is an enhancer of visible | dominant | homeotic phenotype of ash1¹⁷, trx^B11/trx[+]

(Gildea et al., 2000)

E(Pc)¹ is an enhancer of visible | dominant phenotype of Pc⁴

(Sinclair et al., 1998)

Suppressor of

Statement

Reference

E(Pc)[+]/E(Pc)¹ is a suppressor of visible | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

E(Pc)[+]/E(Pc)¹ is a suppressor of visible | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

E(Pc)¹ is a suppressor | partially of increased cell death phenotype of Dref^UAS.cSa, Scer\GAL4^GMR.PS

(Hirose et al., 2001)

E(Pc)¹ is a suppressor of abnormal eye color phenotype of w^T81

(Netter et al., 1998)

NOT Suppressor of

Statement

Reference

E(Pc)¹ is a non-suppressor of visible phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

Other

Statement

Reference

Bap55^UAS.cTa, E(Pc)¹, Scer\GAL4^GH146.PB has abnormal neuroanatomy phenotype

(Tea and Luo, 2011)

E(Pc)¹, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(Pc)[+]/E(Pc)¹, ash1²² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(Pc)[+]/E(Pc)¹, brm² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(Pc)[+]/E(Pc)¹, trx^B11 has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(Pc)¹, brm² has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(Pc)¹, trx^B11 has visible | dominant | homeotic phenotype

(Gildea et al., 2000)

E(Pc)¹, E(Pc)a¹ has visible | homeotic phenotype

Phenotype Manifest In

Enhancer of

Statement

Reference

E(Pc)[+]/E(Pc)¹ is an enhancer of wing vein | adult stage phenotype of Hsap\SMARCAL1^UAS.cBa, Scer\GAL4^Bx-MS1096

(Morimoto et al., 2016)

E(Pc)¹ is an enhancer of wing margin phenotype of Scer\GAL4^bbg-C96, dom^RNAi.Sym.UAS

(Kwon et al., 2013)

E(Pc)¹ is an enhancer of eye phenotype of CycE^JP

(Brumby et al., 2002)

E(Pc)[+]/E(Pc)¹ is an enhancer of adult abdominal segment 4 | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

E(Pc)[+]/E(Pc)¹ is an enhancer of adult abdominal segment 5 phenotype of E(z)^Trm

(Bajusz et al., 2001)

E(Pc)[+]/E(Pc)¹ is an enhancer of adult metathoracic segment phenotype of brm[+]/brm², trx^E2

(Gildea et al., 2000)

E(Pc)[+]/E(Pc)¹ is an enhancer of adult metathoracic segment phenotype of ash1¹⁷, trx^B11/trx[+]

(Gildea et al., 2000)

E(Pc)¹ is an enhancer of leg phenotype of Pc⁴

(Sinclair et al., 1998)

E(Pc)[+]/E(Pc)¹ is an enhancer of sex comb | ectopic phenotype of Pc⁴

(Sinclair et al., 1998)

E(Pc)¹ is an enhancer of phenotype of Pcl¹¹

(Lindsley and Zimm, 1992)

E(Pc)¹ is an enhancer of phenotype of Pcl¹²

(Lindsley and Zimm, 1992)

E(Pc)¹ is an enhancer of phenotype of pho¹

(Lindsley and Zimm, 1992)

E(Pc)¹ is an enhancer of phenotype of esc¹

(Lindsley and Zimm, 1992)

E(Pc)¹ is an enhancer of phenotype of Pcl^unspecified

(Lindsley and Zimm, 1992)

E(Pc)¹ is an enhancer of phenotype of Pc^unspecified

(Lindsley and Zimm, 1992)

Suppressor of

Statement

Reference

E(Pc)[+]/E(Pc)¹ is a suppressor of wing vein | adult stage phenotype of Marcal1^UAS.cBa, Scer\GAL4^Tub.PU

(Morimoto et al., 2016)

E(Pc)[+]/E(Pc)¹ is a suppressor of adult prothoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

E(Pc)[+]/E(Pc)¹ is a suppressor of adult mesothoracic segment | ectopic phenotype of E(z)^Trm

(Bajusz et al., 2001)

E(Pc)[+]/E(Pc)¹ is a suppressor of adult metathoracic segment phenotype of E(z)^Trm

(Bajusz et al., 2001)

E(Pc)¹ is a suppressor | partially of eye phenotype of Dref^UAS.cSa, Scer\GAL4^GMR.PS

(Hirose et al., 2001)

NOT Suppressor of

Statement

Reference

E(Pc)¹ is a non-suppressor of eye phenotype of Myc^UAS.cZa, Scer\GAL4^GMR.PF

(Secombe et al., 2007)

Other

Statement

Reference

Bap55^UAS.cTa, E(Pc)¹, Scer\GAL4^GH146.PB has adult antennal lobe projection neuron DL1 adPN phenotype

(Tea and Luo, 2011)

E(Pc)[+]/E(Pc)¹, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(Pc)[+]/E(Pc)¹, brm² has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(Pc)[+]/E(Pc)¹, trx^B11 has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(Pc)¹, ash1²² has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(Pc)¹, brm² has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(Pc)¹, trx^B11 has adult metathoracic segment phenotype

(Gildea et al., 2000)

E(Pc)¹, pho¹ has embryonic head phenotype

(Lindsley and Zimm, 1992)

E(Pc)¹, E(Pc)a¹ has metathoracic leg phenotype

E(Pc)¹, E(Pc)a¹ has embryonic abdominal segment 1 phenotype

E(Pc)¹, E(Pc)a¹ has embryonic abdominal segment 4 phenotype

E(Pc)¹, E(Pc)a¹ has mesothoracic leg phenotype

Additional Comments

Genetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Marcal1^Scer\UAS.cBa under the control of Scer\GAL4^tub.PU is ameliorated by combination with a single copy of E(Pc)¹.

(Morimoto et al., 2016)

The wing nicking phenotype caused by expression of dom^{dsRNA.Sym.Scer\UAS} under the control of Scer\GAL4^bbg-C96 is enhanced if the flies are also carrying E(Pc)¹.

(Kwon et al., 2013)

Expression of Bap55^Scer\UAS.cTa using Scer\GAL4^GH146.PB in a dom^LL05537 background causes dendrites to mistarget to both DA41 and DM6 glomeruli, and results in axons targeting ventrally to the lateral horn.

(Tea and Luo, 2011)

An E(Pc)¹ background weakly suppresses the rough eye phenotype found in flies expressing Dref^Scer\UAS.cSa under the control of Scer\GAL4^GMR.PS.

(Hirose et al., 2001)

No significant modification of the number of sex combs is seen in E(Pc)¹/+ corto⁴²⁰/+ double heterozygotes compared to E(Pc)¹/+ single heterozygotes.

(Lopez et al., 2001)

Weak suppressor of eye pigment phenotype of w^T81.

(Netter et al., 1998)

Dominantly enhances the extra sex combs phenotype of Pc⁴.

(Sinclair et al., 1998)

E(Pc)¹ acts zygotically as a dominant enhancer of the adult homoeotic syndrome of flies heterozygous for Pc mutations or Df(3L)Pc-MK. Heterozygosity for E(Pc)¹ also enhances the adult homoeotic syndrome of Pcl mutations or Pcl deficiency heterozygotes and renders the normally recessive mutations esc¹ and pho¹ slightly pseudodominant, but has no effect on phenotypes associated with Antp^Ns, Antp^73b, Antp^Scx, Scr^Msc, Ubx^Cbx-1, Ubx¹, Ubx^bx-34e or Ubx^abx-1, Ubx^bx-3, Ubx^pbx-1 heterozygotes. E(Pc)¹ has dominant maternal as well as zygotic effect on the severity of the embryonic homoeotic syndrome of Pc³ or Df(3L)Pc-MK homozygotes. Zygotic homozygosity for E(Pc)¹ also enhances the embryonic effects of Pcl¹¹, Df(2R)Pcl-W5 and Pcl¹², and E(Pc)¹; pho¹ doubly homozygous embryos from heterozygous mothers show incomplete head involution, presumably due to a cryptic homoeotic effect.

(Lindsley and Zimm, 1992)

Xenogenetic Interactions

Statement

Reference

The formation of ectopic wing veins observed in adult flies expressing Hsap\SMARCAL1^Scer\UAS.cBa under the control of Scer\GAL4^Bx-MS1096 is ameliorated by combination with a single copy of E(Pc)¹.

(Morimoto et al., 2016)

Complementation and Rescue Data

Fails to complement

E(Pc)²

(Sinclair et al., 1998)

E(Pc)²

(Stankunas et al., 1998)

Comments

Images (0)

Mutant

Wild-type

Stocks (5)

Bloomington

3142

esc⁵ E(Pc)¹/SM5

3056

cn¹ E(Pc)¹ bw¹/SM5

34491

y¹ w¹; P{neoFRT}40A P{FRT(w^hs)}G13 cn¹ E(Pc)¹ bw¹/CyO

Kyoto

106875

esc⁵ E(Pc)¹/SM5

106808

cn¹ E(Pc)¹ bw¹/SM5

Notes on Origin

Discoverer

Comments

Weak derepression of en expression.

(Moazed and O'Farrell, 1992)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

E(Pc)

(Bajusz et al., 2001, Campbell et al., 1995, Sato et al., 1983)

E(Pc)¹

(Bailetti et al., 2019, Leatham-Jensen et al., 2019, Feng et al., 2018, Feng et al., 2017, Kwon et al., 2013, Tea and Luo, 2011, Christensen et al., 2008.10.20, Secombe et al., 2007, Christensen and Cook, 2006.8.29, Holmes et al., 2006, Hirose et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (30)

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