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General Information
Symbol
Dmel\groE48
Species
D. melanogaster
Name
FlyBase ID
FBal0003339
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
E(spl)E48
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

T26048371A

Amino acid change:

V663D | gro-PA; V663D | gro-PB; V663D | gro-PC; V663D | gro-PD; V674D | gro-PE; V663D | gro-PF; V672D | gro-PG; V653D | gro-PH; V642D | gro-PI; V661D | gro-PJ

Reported amino acid change:

V663D

Comment:

Nucleotide substitution inferred from reported amino acid change. Position of mutation on reference sequence inferred by FlyBase curator.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology

Polytene chromosomes normal.

Nature of the lesion
Statement
Reference

Amino acid replacement: V663D.

Mutations in the m9/m10 coding region.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

35% of heterozygous flies show duplication of the anterior postalar bristle.

gro1/groE48 adult wings show no overt phenotype.

Embryos laid by females containing homozygous germline clones have a strong mutant cuticle phenotype (equivalent to that seen in gro null mutants).

Homozygous clones in the notum produce tufts of bristles.

Heterozygotes have a mild bristle loss phenotype.

Homozygous clones have ectopic microchaetae in the region of the scutellum.

Some zygotically mutant groE48/Df(3R)Espl22 embryos show subtle patterning defects, consistent with mild wg hyperactivation.

Clones fail to differentiate epidermis. Microchaetes are densely packed and adjacent to one another. Macrochaetes are present as dense tufts of abnormal bristles. h22/groE48 double heterozygotes differentiate ectopic microchaete along the wing vein 2.

Clonal analysis revealed that the gro mutant bristle tufting phenotype is epistatic to the H null bristle loss phenotype.

In clones in the wing, the phenotype depends on where the clone lies. In the anterior compartment all clones abutting the wing margin cause local overgrowth and pattern duplications. Those abutting the wing margin and restricted to dorsal or ventral surfaces cause overgrowth of both dorsal and ventral cells. Clones at the wing margin cause loss of sensory organs and mild scalloping of the margin.

Heterozygotes that are also heterozygous for groBFP2 have eye defects that are similar but not identical to groBFP2 homozygotes. Homozygous mutant clones in the eye show ommatidial defects, often having extra photoreceptors.

Weak neurogenic phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference

groE48 has visible | dominant phenotype, suppressible | partially by klu[+]/klu212lR51C

Enhancer of
Statement
Reference

groE48/gro[+] is an enhancer of visible | heat sensitive phenotype of HC2.hs

Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

groE48 has phenotype, enhanceable by kni9

groE48 has phenotype, enhanceable by kni2

Suppressed by
Statement
Reference

groE48 has microchaeta | ectopic | somatic clone phenotype, suppressible by NMcd1

NOT suppressed by
Statement
Reference

groE48 has microchaeta | ectopic | somatic clone phenotype, non-suppressible by NAx-59b

Enhancer of
Statement
Reference

groE48 is an enhancer of phenotype of kni9

groE48 is an enhancer of phenotype of kni2

groE48/gro[+] is an enhancer of wing vein | ectopic | heat sensitive phenotype of HC2.hs

groE48/gro[+] is an enhancer of wing | heat sensitive phenotype of HC2.hs

NOT Enhancer of
Statement
Reference

groE48 is a non-enhancer of wing phenotype of Scer\GAL4bbg-C96, mamN.UAS

Suppressor of
Statement
Reference

groE48 is a suppressor of phenotype of arm4

groE48 is a suppressor of phenotype of arm3

Other
Additional Comments
Genetic Interactions
Statement
Reference

54% of groE48/klu212lR51C double heterozygotes show loss of the presutural bristle.

klu212lR51C/+ partially suppresses the duplication of the anterior postalar bristle seen in groE48 heterozygotes: only 12% of the double heterozygous flies show the duplication.

gro1/groE48 adults heterozygous for sbb6 show an enlarged anterior wing compartment. Ectopic longitudinal wing vein L2 and L3 tissue develops, and transformation of the anterior wing margin bristles into more posteriorly located sensory organs is observed.

The loss of muscles that is seen in embryos expressing HimScer\UAS.cLa under the control of Scer\GAL4twi.PB is suppressed if the embryos are also mutant for groE48/Df(3R)Espl22.

groE48 clones induced in flies expressing HLHm7Scer\UAS.cdCa under the control of Scer\GAL4ap-md544 result in patches of high bristle density in a bald notum.

The partial lethality due to runScer\UAS.cLa; Scer\GAL4nos.PG (3% viable) is partially suppressed by maternal heterozygosity for groE48 (rescues to 30% viable).

The severity of cuticle defects seen in eve1 embryos is increased if the mother is also heterozygous for groE48. The defects are pair rule in nature. The degree of rescue of the eve3 embryonic phenotype conferred by one copy of eveE+L.8.4 is reduced if the mother is heterozygous for groE48. The degree of rescue of the eve3 embryonic phenotype conferred by two copies of eveEGNΔLFK is reduced slightly if the mother is heterozygous for groE48.

NMcd1 groE48 double mutant clones lack the ectopic microchaetae seen in groE48 clones. NAx-59b groE48 double mutant clones do form ectopic microchaetae (as is seen in groE48 single mutant clones).

groE48/osa308 transheterozygous adults have notum to wing transformations.

Eggs from Rpd304556/groE48 mothers exhibit an embryonic lethality significantly higher than from either groE48/+ or Rpd304556/+ mothers. Cuticles from unhatched embryos show a bicaudal phenotype, including a duplicated posterior spiracle, and a mirror image duplication of the posterior abdominal segments in place of the normal anterior segments. Eggs from Df(3L)10H/groE48 mothers exhibit an embryonic lethality significantly higher than from either groE48/+ or Df(3L)10H/+ mothers. 50-60% had no cuticle. Cuticles from 5-10% of unhatched embryos show a duplication of the posterior spiracle accompanied by disorganised denticle belts.

groE48 has no effect on the wing nicking phenotype seen in mamN.Scer\UAS, Scer\GAL4C96 flies.

Suppression of arm mutant phenotypes depends on reduction of maternal contribution from the gro locus. Suppression of panΔN.Scer\UAS mutant phenotype depends on reduction of maternal contribution from the gro locus.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Can be rescued by a grounspecified transgene.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Preiss.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (57)