|Feature type||allele||Associated gene||Dmel\gro|
|Also Known As||E(spl)E48|
|Map ( GBrowse )|
|Allele class||amorphic allele - genetic evidence, loss of function allele, hypomorphic allele - genetic evidence|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
pr_change=V663D | gro-PB; V663D | gro-PA; V663D | gro-PD; V663D | gro-PC; V674D | gro-PE; V663D | gro-PF; V672D | gro-PG; V653D | gro-PH
comment=Nucleotide substitution inferred from reported amino acid change. Position of mutation on reference sequence inferred by FlyBase curator.
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: V663D.
Mutations in the m9/m10 coding region.
Polytene chromosomes normal.
|Phenotype Manifest In|
macrochaeta & scutum
microchaeta & scutum
sense organ & wing
35% of heterozygous flies show duplication of the anterior postalar bristle.
Embryos laid by females containing homozygous germline clones have a strong mutant cuticle phenotype (equivalent to that seen in gro null mutants).
Homozygous clones in the notum produce tufts of bristles.
Heterozygotes have a mild bristle loss phenotype.
Homozygous clones have ectopic microchaetae in the region of the scutellum.
Some zygotically mutant groE48/Df(3R)Espl22 embryos show subtle patterning defects, consistent with mild wg hyperactivation.
Clones fail to differentiate epidermis. Microchaetes are densely packed and adjacent to one another. Macrochaetes are present as dense tufts of abnormal bristles. h22/groE48 double heterozygotes differentiate ectopic microchaete along the wing vein 2.
Clonal analysis revealed that the gro mutant bristle tufting phenotype is epistatic to the H null bristle loss phenotype.
In clones in the wing, the phenotype depends on where the clone lies. In the anterior compartment all clones abutting the wing margin cause local overgrowth and pattern duplications. Those abutting the wing margin and restricted to dorsal or ventral surfaces cause overgrowth of both dorsal and ventral cells. Clones at the wing margin cause loss of sensory organs and mild scalloping of the margin.
Heterozygotes that are also heterozygous for groBFP2 have eye defects that are similar but not identical to groBFP2 homozygotes. Homozygous mutant clones in the eye show ommatidial defects, often having extra photoreceptors.
Weak neurogenic phenotype.
|Phenotype Manifest In|
|NOT suppressed by|
groE48/gro[+] is an enhancer | maternal effect of embryonic/first instar larval cuticle phenotype of eve1
|NOT Enhancer of|
Df(3R)Espl22/groE48 is a suppressor of embryonic/larval muscle system phenotype of HimScer\UAS.cLa, Scer\GAL4twi.PB
54% of gro[E48]/klu[212lR51C] double heterozygotes show loss of the presutural bristle. klu[212lR51C]/+ partially suppresses the duplication of the anterior postalar bristle seen in gro[E48] heterozygotes: only 12% of the double heterozygous flies show the duplication.
gro/gro[E48] adults heterozygous for sbb show an enlarged anterior wing compartment. Ectopic longitudinal wing vein L2 and L3 tissue develops, and transformation of the anterior wing margin bristles into more posteriorly located sensory organs is observed.
The loss of muscles that is seen in embryos expressing HimScer\UAS.cLa under the control of Scer\GAL4twi.PB is suppressed if the embryos are also mutant for groE48/Df(3R)Espl22.
groE48 clones induced in flies expressing HLHm7Scer\UAS.cdCa under the control of Scer\GAL4ap-md544 result in patches of high bristle density in a bald notum.
The partial lethality due to runScer\UAS.cLa; Scer\GAL4nos.PG (3% viable) is partially suppressed by maternal heterozygosity for groE48 (rescues to 30% viable).
The severity of cuticle defects seen in eve1 embryos is increased if the mother is also heterozygous for groE48. The defects are pair rule in nature. The degree of rescue of the eve3 embryonic phenotype conferred by one copy of eveE+L.8.4 is reduced if the mother is heterozygous for groE48. The degree of rescue of the eve3 embryonic phenotype conferred by two copies of eveEGNΔLFK is reduced slightly if the mother is heterozygous for groE48.
NMcd1 groE48 double mutant clones lack the ectopic microchaetae seen in groE48 clones. NAx-59b groE48 double mutant clones do form ectopic microchaetae (as is seen in groE48 single mutant clones).
Eggs from Rpd304556/groE48 mothers exhibit an embryonic lethality significantly higher than from either groE48/+ or Rpd304556/+ mothers. Cuticles from unhatched embryos show a bicaudal phenotype, including a duplicated posterior spiracle, and a mirror image duplication of the posterior abdominal segments in place of the normal anterior segments. Eggs from Df(3L)10H/groE48 mothers exhibit an embryonic lethality significantly higher than from either groE48/+ or Df(3L)10H/+ mothers. 50-60% had no cuticle. Cuticles from 5-10% of unhatched embryos show a duplication of the posterior spiracle accompanied by disorganised denticle belts.
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 6 )|
|Secondary FlyBase IDs|
|References ( 52 )|
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|Recent research papers ( 3 )|