FB2025_01 , released February 20, 2025
Allele: Dmel\ea1
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General Information
Symbol
Dmel\ea1
Species
D. melanogaster
Name
FlyBase ID
FBal0003455
Feature type
allele
Associated gene
Dmel\ea
Associated Insertion(s)
Carried in Construct
Key Links
Genomic Maps

Allele class

amorphic allele - genetic evidence

gain of function allele

Mutagen
Nature of the Allele
Allele class

amorphic allele - genetic evidence

(Ray et al., 1991, Chasan and Anderson, 1989)

gain of function allele

(Ray et al., 1991)
Mutagen
ethyl methanesulfonate
(Tearle and Nusslein-Volhard, 1987)
Progenitor genotype
Cytology
Description

Point mutation in the catalytic site.

(Lemaitre et al., 1996)

Amino acid replacement: G339R.

(Jin and Anderson, 1990)

Nucleotide substitution: G1218A.

(Jin and Anderson, 1990)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:15,330,197..15,330,197 [+]
Nucleotide change:

G15330197A

Reported nucleotide change:

G1218A

Amino acid change:

G339R | ea-PA; G208R | ea-PB

Reported amino acid change:

G339R

(Jin and Anderson, 1990)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      The mean ln (natural logarithm) circulating hemocyte concentration (CHC) value of ea1/ea3 larvae is statistically indistinguishable from that of control siblings. The mean ln (natural logarithm) circulating hemocyte concentration (CHC) value of homozygous larvae is significantly lower than that of control siblings. The ability of ea1/ea3 larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae. The ability of homozygous larvae to encapsulate L.boulardi eggs is significantly reduced compared to that of control larvae.

      (Sorrentino et al., 2004)

      Bacterially challenged mutants exhibit a wild type Drs response. Pattern of response of CecA1 and CecA2 parallels that of Drs. Dpt and Dro remain fully inducible and pattern of expression of AttA and Def in intermediate. Infection of ea1/ea2 mutants with A.fumigatus causes higher survival rate than wild type, infection with E.coli causes 87% survival 3 days postinfection.

      (Lemaitre et al., 1996)

      Heterozygotes are female sterile.

      (Belvin et al., 1995)

      Injection of snkΔne.T:ea into the central region of homozygous embryos causes a dorsalized phenotype.

      (Smith and DeLotto, 1994)

      Perivitelline fluid from Tl and dl donors was capable of restoring polarized gastrulation movements and cuticular elements. Perivitelline fluid transplantation using eaD8 embryos as donors and ea1/ea2 embryos as recipients demonstrated that the rescue of the ea phenotype results from the transplantation of the ea gene product.

      (Stein and Nusslein-Volhard, 1992)

      Does not interact with RpII140wimp maternal effect.

      (Parkhurst and Ish-Horowicz, 1991)

      Ventralized embryos: rings or patches of ventral denticles along dorsoventral axis. Altered pattern of dpp and zen.

      (Ray et al., 1991)

      Embryos strongly dorsalized.

      (Jin and Anderson, 1990)

      Homozygous embryos have a strongly dorsalized phenotype: dorsal cuticle differentiates around the entire dorso-ventral circumference. ea1/ea7 heterozygotes at 18oC have a moderately dorsalized phenotype: very reduced denticle belts. ea1/ea3 heterozygotes at 18oC have a weakly dorsalized phenotype: ventral denticle belts are of nearly normal width and there are abnormalities in the head skeleton.

      (Chasan and Anderson, 1989)

      strong allele

      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Enhancer of
      Statement
      Reference

      ea1/ea[+] is an enhancer of embryonic epidermis phenotype of cactE10

      (Wakabayashi-Ito et al., 2001)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Embryos derived from Spn27Aex32/Df(2L)BSC7 ; ea1/ea2 females are completely dorsalised.

      (Ligoxygakis et al., 2003)

      Perivitelline fluid from embryos laid by eaD3 spz4/ea1 spz2 females does not show axis-inducing activity when injected into the perivitelline space of embryos derived from pip1/pip2 females.

      (Chang and Morisato, 2002)

      50% of embryos produced by cactE10/+, ea1/+ are weakly dorsalized.

      (Wakabayashi-Ito et al., 2001)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (4)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (1)
      Reported As
      Symbol Synonym
      ea1
      (Alpar et al., 2018, Cho et al., 2010, Cook et al., 2009.2.28, Leclerc et al., 2006, DeLotto, 2001, Jin and Anderson, 1990)
      Name Synonyms
      Secondary FlyBase IDs
        References (25)