FB2025_01 , released February 20, 2025
Allele: Dmel\eaD1
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General Information
Symbol
Dmel\eaD1
Species
D. melanogaster
Name
FlyBase ID
FBal0003474
Feature type
allele
Associated gene
Dmel\ea
Associated Insertion(s)
Carried in Construct
Also Known As
ea5.13
Key Links
Genomic Maps

Allele class

gain of function allele

Mutagen
Nature of the Allele
Allele class

gain of function allele

(Chasan and Anderson, 1989)
Mutagen
ethyl methanesulfonate
Progenitor genotype
Cytology
Description

Amino acid replacement: V360M.

(Jin and Anderson, 1990)

Nucleotide substitution: G1281A.

(Jin and Anderson, 1990)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
3R:15,330,260..15,330,260 [+]
Nucleotide change:

G15330260A

Reported nucleotide change:

G1281A

Amino acid change:

V360M | ea-PA; V229M | ea-PB

Reported amino acid change:

V360M

(Jin and Anderson, 1990)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In
      Detailed Description
      Statement
      Reference

      Embryos laid by heterozygous females are either lateralised, developing a cuticle with rings of ventral and lateral denticle bands. At gastrulation, embryos laid by heterozygous females fail to invaginate a ventral furrow and have a widened head fold. Embryos laid by eaD1/Df(3R)ea-5022rx1 females are markedly more elongated and have fewer denticle bands than embryos laid by eaD1/+ females.

      (Chang and Morisato, 2002)

      Additional dl protein in gain of function dorsal group backgrounds shifts the nuclear dl gradient ventrally.

      (Govind et al., 1993)

      Lateralized embryos: cuticle is an elongated tube covered with rings of denticles. dpp, zen and twi are only expressed at the poles, only transient expression of sna is seen.

      (Ray et al., 1991)

      Embryos lateralized.

      (Jin and Anderson, 1990)

      Embryos have a lateralized phenotype: differentiate rings of ventral denticles around the entire dorso-lateral circumference and do not form the ventrally derived mesoderm. Heterozygous females are sterile.

      (Chasan and Anderson, 1989)

      Dominant allele. Embryos are lateralised.

      (Tearle and Nusslein-Volhard, 1987)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Suppressed by
      Statement
      Reference

      eaD1 has phenotype, suppressible by Tlrv13

      (Chasan et al., 1992)

      eaD1 has phenotype, suppressible by Tlrv19

      (Chasan et al., 1992)
      Additional Comments
      Genetic Interactions
      Statement
      Reference

      Perivitelline fluid from embryos laid by eaD1 spz2/ea4 spz4 females shows axis-inducing activity when injected into the the perivitelline space of embryos derived from pip1/pip2 females.

      (Chang and Morisato, 2002)
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments

      9 EMS revertants and 2 X ray revertants recovered

      Images (0)
      Mutant
      Wild-type
      Stocks (0)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (2)
      References (12)