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General Information
Symbol
Dmel\ecd2
Species
D. melanogaster
Name
FlyBase ID
FBal0003501
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C2265574T

Reported nucleotide change:

C?T

Amino acid change:

Q68term | ecd-PA

Reported amino acid change:

Q67term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q67term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

ecd2 homozygotes, ecd2/Df(3L)R-R2 and ecd2/ecdl(3)23 animals die after a prolonged L2. Most ecd1/ecd2 animals die during the L2/L3 molt, displaying typical molting defects such as double mouth hooks, but some survive into L3. Larval Lethality in ecd2 homozygotes is not rescued by feeding with 20-hydroxyecdysone (50 μg/ml or 250 μg/ml). ecd2 homozygous somatic clones induced at 24-36 hours after egg laying do not survive in wing or eye discs to late third instar. The resulting adults appear normal. However, such clones induced at the onset of the third instar do survive in late third instar eye discs. ecd2 homozygous clones in the ovary cause egg chamber fusions, typically producing egg chambers with double the normal number of germ cells. Egg chambers in which all germ cells are part of ecd2 homozygous germ-line clones arrest development early in oogenesis, showing degeneration of nurse cells, resulting in female sterility.

Hemizygous larvae die 48 to 72 hours after hatching, failing to survive the moult to the second instar stage. ecd1/ecd2 transheterozygotes die as they moult to the third larval instar; most die during ecdysis, with the old cuticle often remaining attached to the posterior end of the abdomen. The number of macrochaetae on the thorax is reduced in ecd1/ecd2 flies kept at 29oC in the hours that follow prepupal formation.

Temperature upshift at egg-laying or early third instar caused larval lethality. Temperature upshift at puparium formation causes pharate adult lethality.

nonconditional allele

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by
Comments

ecdScer\UAS.cGa with Scer\GAL4Feb36, Scer\GAL4Aug21, Scer\GAL4en-e16E or Scer\GAL4hs.2sev does not rescue larval lethality of ecd2/ecd2 animals even as far as the second (L2/L3) molt. By contrast ecdScer\UAS.cGa with Scer\GAL4Act5C.PP rescues ecd2/ecd2 animals to adulthood, and ecdScer\UAS.cGa with 2XScer\GAL4ptc-559.1 partially rescues: larvae molt to the third instar and then form defective but tanned prepupae.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
ecd2
Name Synonyms
Secondary FlyBase IDs
    References (5)