|Feature type||allele||Associated gene||Dmel\Egfr|
|Also Known As||Elp1, egfrElp, Elp, Ellipse1, dEGFRElp, DERElp1, EgfrE|
|Map ( GBrowse )|
|Allele class||hypermorphic allele - genetic evidence, gain of function allele|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
comment=One of two nucleotide substitutions affecting the amino acid sequence in the EgfrE1 mutant relative to the reference sequence. A L21W change is also reported in FBrf0105872 but a W already exists at this location in the reference sequence.
|Associated Sequence Data|
|Nature of the lesion|
Amino acid replacement: S5R. Amino acid replacement: L21W. Amino acid replacement: A887T. Figure 1 and the text concerning this allele are at odds - the text is correct (note from N. Baker).
(Rusconi et al., 2004, Li and Li, 2003, Kumar et al., 1998, Shamloula et al., 2002, Lamb et al., 1998, Spencer and Cagan, 2003, Ashton-Beaucage et al., 2010, Baker and Rubin, 1992, Brunner et al., 1994, Kotarski et al., 1998, Rogge et al., 1991, Read et al., 2005, Spencer et al., 1998, Iyadurai et al., 2008, Haberman et al., 2010)
|Phenotype Manifest In|
(Kolodkin et al., 1994, Kumar et al., 1998, Shamloula et al., 2002, Ghiglione et al., 2003, Lamb et al., 1998, Spencer and Cagan, 2003, Polaczyk et al., 1998, Baker and Rubin, 1992, Brunner et al., 1994, Kotarski et al., 1998, Rogge et al., 1991, Frankfort and Mardon, 2004, Read et al., 2005, Spencer et al., 1998, Sullivan and Rubin, 2002, Iyadurai et al., 2008, Haberman et al., 2010)
Mutant flies have an extra wing vein phenotype with an additional vein fragment above vein L2 and some vein deltas.
Heterozygotes have extra wing vein material in the wing.
In Egfr[E1]/+ adults, the number of ommatidia with pale-type R8 cells and yellow-type R7 cells is significantly increased. Many of these are found in pale-yellow hybrid ommatidia with a yellow-type R7 and a pale-type R8.
Heterozygotes have a rough eye phenotype, with elliptical eyes and disarranged ommatidial arrays, and wing vein L2 shows an abnormal broadening at the wing margin.
EgfrE1 homozygous adults are small and have greatly reduced numbers of ommatidia with large gaps of pigmented tissue between them. Heterozygous adults have normal eyes.
Heterozygous adults have smaller eyes than normal which have moderate rough phenotype.
Wings of heterozygous and homozygous flies have extra wing vein material.
Heterozygotes have smaller eyes than normal which are mispatterned.
Heterozygous mutants do not cause any defects in the ocellar sensory system (OSS) neurogenesis or axon guidance.
Heterozygotes have smaller eyes than normal and lack some ommatidia, producing an irregular arrangement of facets.
Heterozygotes have rough eyes. This phenotype is suppressed towards wild-type in transheterozygous combination with Egfrf24. This phenotype is suppressed towards wild-type in transheterozygous combination with Egfrtsla at 28oC but not at 18oC. Heterozygotes show greatly reduced ommatidial cluster formation in the morphogenetic furrow of the eye disc.
Heterozygotes have a rough eye phenotype.
Heterozygotes exhibit roughening of patches on both eyes. Homozygotes exhibit strong blistering, including loss of pigmentation, in patches on both eyes. Rhabdomere assembly is disordered with extra material at the apical ends of the ommatidia. Ommatidia pattern is disordered.
Heterozygotes have rough eyes.
Homozygous flies have a few, widely separated ommatidia. Each ommatidium contains a single R8 cell, as in wild-type flies, and this phenotype is not altered in scaWB1/scaOB7 EgfrE3/EgfrE1 double homozygotes.
Heterozygotes have a mild rough eye phenotype due to a disrupted ommatidial array.
Displays locomotor activity rhythm with an approximately 24h period.
Extra wing veins close to the wing margin.
Rough eye phenotype: eyes are small and rough at the perimeter, ommatidia and hairs are in an irregular pattern.
Heterozygous rhove-1 dominantly suppresses the homozygous and heterozygous phenotype. Heterozygous rhoWk, rhoMod and rhoStg enhance the heterozygous phenotype.
Homozygous eye discs have fewer ommatidia than wild-type. Outgrowth of photoreceptor axons is disturbed, occurring in many directions. Cell death is abundant in the region approximately corresponding to columns 10-15 in wild-type. In the adult eye the ommatidia are separated by numerous pigment cells and mechanosensory bristles. Wing veins show thickening and plexus formation. Enhances the wing phenotype of Df(1)N-54l9, N264-39, Dl9P, and DlKX6 mutations, and the eye phenotype of Nspl-1.
Mild roughening of the eye.
Homozygotes have fewer ommatidia than wild-type.
The array of ommatidia is less regular in heterozygotes than in wild-type flies, and there is a slight disturbance in the wing vein pattern. Homozygotes have much smaller eyes than normal, containing many fewer ommatidia, and some regions lack ommatidia entirely. These regions contain cells resembling the pigment cells that normally surround each ommatidium, and also mechanosensory bristles. Most ommatidia contain the normal number and arrangement of cells. Only approximately one tenth of the normal number of preclusters differentiate in the eye imaginal discs.
Eyes of EgfrE1/+ heterozygotes rough and more oval than wild type; also display a slight disturbance of the wing-vein pattern. Homozygotes have smaller eyes with many fewer ommatidia and some regions lack them entirely; those ommatidia that are formed contain the normal number and arrangement of cells; the regions without ommatidia contain cells that resemble pigment cells and mechanosensory bristles; only about one tenth the normal number of preommatidial cell clusters differentiate behind the morphogenetic furrow. EgfrE1 in heterozygous combination with a deficiency or null mutation for Egfr is normal in phenotype indicating that EgfrE1 alleles are hypermorphic.
|NOT Enhanced by|
|NOT suppressed by|
|NOT Enhancer of|
|NOT Suppressor of|
|Phenotype Manifest In|
|NOT Enhanced by|
|NOT suppressed by|
EgfrE1 has photoreceptor cell R8 of pale ommatidium phenotype, non-suppressible | somatic clone by rhoPΔ5/rhoPΔ5
Egfr[+]/EgfrE1 is an enhancer of wing vein | ectopic phenotype of Pi3K68DKD.Scer\UAS.T:Hsap\MYC, Scer\GAL469B
|NOT Enhancer of|
|NOT Suppressor of|
Expression of CG4096[KK108644] under the control of Scer\GAL4[Act5C.PU] enhances the extra wing vein phenotype of Egfr[E1] flies. Expression of Sulf1[Scer\UAS.cBa] under the control of Scer\GAL4[Act5C.PU] suppresses the extra wing vein phenotype of Egfr[E1] flies.
The extra wing vein phenotype seen in Egfr[E1]/+ flies is dominantly suppressed by rl or mago.
In Egfr[E1]/+ adults, the number of ommatidia with pale-type R8 cells and yellow-type R7 cells is significantly increased. Many of these are found in pale-yellow hybrid ommatidia with a yellow-type R7 and a pale-type R8. This phenotype is suppressed by sev/sev, but not by rho[PΔ5] homozygous somatic clones.
Dhc64C[8-1] dominantly enhances the eye phenotype of Egfr[E1]/+ flies, resulting in a reduced, narrower eye with fewer ommatidia. The abnormal broadening of wing vein L2 at the wing margin which is seen in Egfr[E1]/+ flies is suppressed by Gl/+.
An Egfr[E1] heterozygous background gives a dominant enhancement to the ato/Df(3R)p13 eye phenotype at 25[o]C.
A Ero1L[23T] heterozygous background does not affect the ectopic wing vein formation phenotype found in Egfr[E1] homozygous mutants.
Adult eyes containing sensE2 homozygous somatic clones induced in an EgfrE1/+ background are smaller than wild-type. Within the clone there are reduced numbers of ommatidia, as well as gaps of tissue between ommatidia, a phenotype similar to that seen in EgfrE1 homozygotes. sensScer\UAS.cNa; Scer\GAL4ey.PB partially rescues eye size and ommatidial number, and completely suppresses the formation of undifferentiated regions in EgfrE1 homozygous adult eyes.
Egfr[E1]/+ enhances the formation of an ectopic crossvein in flies expressing Pi3K68D[KD.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[69B] at 25[o]C. Flies expressing Pi3K68D[Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[69B] in a Egfr[E1]/+ background at 25[o]C show loss of the anterior and partial loss of the posterior crossvein.
The rough eye phenotype of EgfrE1/+ flies is strongly enhanced by homozygosity for kek1unspecified. These flies also have small eyes and more extra vein material than EgfrE1/+ flies.
edk01102/+ strongly enhances the EgfrE1/+ phenotype, resulting in eyes that are approximately 1/3 the size of those of EgfrE1/+ flies.
EgfrE1/+ flies carrying Pp2B-14Dact.GMR and CanBGMR.PS have a rough eye phenotype similar to that caused by Pp2B-14Dact.GMR and CanBGMR.PS in an Egfr+ background.
The number of ommatidia in the eyes of EgfrE1/EgfrE3 adults is not altered if the flies are also expressing BacA\p35GMR.PH, but the number of pigment cells is increased in these animals.
Expression of p38bDN.Scer\UAS under the control of Scer\GAL4unspecified has no significant effect on the wing phenotype caused by EgfrE1.
argosW11 enhances the extra wing vein phenotype of EgfrE1/EgfrE3 transheterozygotes but suppresses the eye phenotype. Nl1N-ts1 also suppresses the eye phenotype of EgfrE1/EgfrE3.
The EgfrE1/+ rough eye phenotype is completely suppressed by cora12 or cora15. The EgfrE1/+ phenotype is partially suppressed by cora1, cora2 or cora13; the eye is wild type in size with some roughening. The EgfrE1/+ phenotype is partially suppressed by cora4, cora5, cora6 or cora14; the eye is smaller than wild type with some roughening. The EgfrE1/+ rough eye phenotype is slightly suppressed by cora3, cora7, cora8 or cora10. The EgfrE1/+ rough eye phenotype is not dominantly suppressed by cora9 or cora11.
Suppresses the viability of vnddd-13, the proliferation defects in vnL6 and (partially) the small disc phenotype of vnγ4.
Introduction of one copy of aope2d significantly enhances the EgfrE1/+ phenotype, eyes are severely defective, reduced in size and very rough. aop1 and aop2 give a similar enhancement of the mutant eye phenotype.
The heterozygous extra wing vein phenotype is enhanced when transheterozygous with rlSem. Flies heterozygous for EgfrE1 and rlSem have smaller eyes than EgfrE1 heterozygotes alone.
Eye of double heterozygotes with S1 are dramatically reduced with respect to those of either heterozygote alone. When heterozygous for Df(2L)ast2 (removing S and ast) the reduced phenotype becomes much more pronounced.
|Complementation & Rescue Data|
|Stocks ( 12 )|
|Notes on Origin|
E.H. Grell, Feb. 1957.
Phenotype is highly sensitive to the wild-type genetic background.
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 19 )|
(Garcia-Alonso et al., 2000, Kumar and Moses, 2000, Chen and Chien, 1999, Adachi-Yamada et al., 1999, Lesokhin et al., 1999, Lim et al., 1997, Schweitzer et al., 1995, Baker and Zitron, 1995, Baker and Rubin, 1992, Rogge et al., 1991, Diaz-Benjumea and Garcia-Bellido, 1990, Baker and Rubin, 1989, Almudi et al., 2009, Iyadurai et al., 2008)
(Frankfort and Mardon, 2002, Garcia-Alonso, 1999, Gallio et al., 1999, Kumar and Moses, 1998, Lamb and Fehon, 1997, Moses et al., 1997, Bach et al., 2003, Baker et al., 2002, Shamloula et al., 2002, Tang et al., 2001, Hsu et al., 1998, Verheyen et al., 1996, Rogge et al., 1995, Doberstein et al., 1995, Raz et al., 1991, )
|Secondary FlyBase IDs|
|References ( 82 )|
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|Recent research papers ( 1 )|
|Recent reviews (0)|
|All reviews listed in FlyBase were published before 2011|