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General Information
Symbol
Dmel\EgfrE3
Species
D. melanogaster
Name
FlyBase ID
FBal0003528
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ElpB1, EgfrElpB1, DERelpB1, EgfrEB1
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

T21523131C

Reported nucleotide change:

T43C

Amino acid change:

W15R | Egfr-PA

Reported amino acid change:

W15R

Comment:

One of three nucleotide substitutions affecting the amino acid sequence in the EgfrE3 mutant relative to the reference sequence.

point mutation
Nucleotide change:

G21557607A

Reported nucleotide change:

G3165A

Amino acid change:

A887T | Egfr-PA; A936T | Egfr-PB

Reported amino acid change:

A887T

Comment:

One of three nucleotide substitutions affecting the amino acid sequence in the EgfrE3 mutant relative to the reference sequence.

point mutation
Nucleotide change:

C21523158G

Reported nucleotide change:

C70G

Amino acid change:

L24V | Egfr-PA

Reported amino acid change:

L24V

Comment:

One of three nucleotide substitutions affecting the amino acid sequence in the EgfrE3 mutant relative to the reference sequence.

Associated Sequence Data
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: W15R. Amino acid replacement: L24V. Amino acid replacement: A887T. Figure 1 and the text concerning this allele are at odds - the text is correct (note from N. Baker).

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

EgfrE3/+ produces a small rough eye phenotype compared with wild-type.

Adult wings carrying EgfrE3 mutations exhibit extra wing veins.

EgfrE3/+ adults have extra vein tissue at the distal end of L2.

EgfrE3/+ mutants have eyes that are reduced in size with fewer photoreceptors than wild type. Additionally these mutants have, on average, more than two extra crossveins at the tip of L2 in the wing. EgfrE3/Egfrt1 transheterozygotes show a less severe wing phenotype, with the majority of flies exhibiting either no, or one extra crossvein. This phenotype is reduced further in EgfrE3/Egfrtop-18A transheterozygotes, with the vast majority of flies showing no extra crossveins. Flies that are EgfrE3/+ and also carry the deficiency Df(3L)Ar14-8 have a slightly reduced wing phenotype, with a quarter of flies exhibiting no extra crossveins, while a quarter of these flies still exhibit more than two extra crossveins in the wing.

Eye of EgfrE3/+ heterozygotes is rough. Extra wing veins form.

Few ommatidia are seen in homozygous eye discs, in a background of G2-arrested cells. Some mitotic cells are seen adjacent to differentiating ommatidia. Apoptotic cells are seen in columns 7-15 of the developing eye disc.

Mutant flies develop wing with ectopic wing material. EgfrE3 dominantly causes an abnormal eye phenotype. The eye is rough lacking the occasional outer photoreceptor.

Heterozygous mutant wings have ectopic wing vein material.

Heterozygous mutants do not cause any defects in the ocellar sensory system (OSS) neurogenesis or axon guidance.

Heterozygous flies have rough eyes and show a slight overproduction of wing vein material. The eyes of homozygotes are severely reduced in size and contain very few ommatidia. Homozygotes are poorly viable.

Heterozygotes occasionally lack some outer photoreceptor cells. This phenotype is not significantly altered if the flies are also homozygous for Dsor1Su1 or Dsor1Su7.

Homozygous embryos have slightly more midline glia cells than normal, at least until stage 15. Homozygous first instar larvae have a normal number of midline glia.

Homozygous flies have a few, widely separated ommatidia. Each ommatidium contains a single R8 cell, as in wild-type flies, and this phenotype is not altered in scaWB1/scaOB7 EgfrE3/EgfrE1 double homozygotes.

Extra wing veins close to the wing margin.

Clonal analysis reveals phenotypes in the adult including loss of wing vein, ectopic wing vein, reduced cell size, extra bristles, cell lethality and tergite bristle abnormalities. Phenotype is cell autonomous. rl1/Df(2R)rl10a suppresses the wing phenotype of EgfrE3.

Rough eye phenotype, which is characterised by disorganised ommatidia and reduction in the size of the eye.

Eyes are reduced and roughened.

Eyes contain only 10% of normal ommatidial complement. This is first evident in the imaginal disc where areas of ommatidia are separated by large distances creating completely isolated groups of ommatidia. Axons from these isolated photoreceptor clusters with no near neighbours can project to the larval brain via the optic stalk.

Homozygous clones in the eye disc are rescued by surrounding heterozygous or wild type cells.

Homozygous eye discs have fewer ommatidia than wild-type. Outgrowth of photoreceptor axons is disturbed, occurring in many directions. Cell death is abundant in the region approximately corresponding to columns 10-15 in wild-type. The pattern of cell division in the eye disc is altered. In the adult eye the ommatidia are separated by numerous pigment cells and mechanosensory bristles. Wing veins show thickening and plexus formation. The eye and wing phenotype is more extreme in the homozygote than the heterozygote.

A morphogenetic furrow crosses the eye disc in homozygotes, but very few ommatidia form. Adults have smaller eyes than normal, with a small number of ommatidia, many of which have the normal cellular constitution. Ommatidia are separated by pigment cells and bristle organs.

The array of ommatidia is less regular in heterozygotes than in wild-type flies, and there is a slight disturbance in the wing vein pattern. Homozygotes have much smaller eyes than normal, containing many fewer ommatidia, and some regions lack ommatidia entirely. These regions contain cells resembling the pigment cells that normally surround each ommatidium, and also mechanosensory bristles. Most ommatidia contain the normal number and arrangement of cells.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

EgfrE3 has visible | dominant phenotype, enhanceable by geminin[+]/geminink03202b

EgfrE3 has visible | dominant phenotype, enhanceable by Gap1[+]/RasGAP1B2

EgfrE3 has visible | dominant phenotype, enhanceable by ed[+]/edlF20

EgfrE3 has visible | dominant phenotype, enhanceable by ed[+]/ed1X5

EgfrE3/EgfrE1 has visible phenotype, enhanceable by aosW11

Suppressed by
Statement
Reference

EgfrE3 has visible | dominant phenotype, suppressible by brmK804R.Tag:HA/brm[+]

EgfrE3 has visible | dominant phenotype, suppressible by brm[+]/brm2

EgfrE3 has visible | dominant phenotype, suppressible by kay2

EgfrE3 has visible | semidominant phenotype, suppressible by ShcBG/Shc[+]

EgfrE3 has visible | semidominant phenotype, suppressible by Shc111-40/Shc[+]

EgfrE3/EgfrE1 has visible phenotype, suppressible by Nl1N-ts1

EgfrE3/EgfrE1 has visible phenotype, suppressible by aosW11

NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

EgfrE3/Egfr[+] is an enhancer of visible phenotype of Mkp31/Mkp32, Scer\GAL4[-]

Suppressor of
Statement
Reference

EgfrE3/Egfr[+] is a suppressor of visible phenotype of CycEJP

Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

EgfrE3 has eye phenotype, enhanceable by geminin[+]/geminink03202b

EgfrE3 has wing phenotype, enhanceable by ash2S112411/ash2S112411

EgfrE3 has wing vein | supernumerary phenotype, enhanceable by ed[+]/ed1X5

EgfrE3 has eye phenotype, enhanceable by Gap1[+]/RasGAP1B2

EgfrE3 has eye phenotype, enhanceable by ed[+]/edlF20

EgfrE3 has eye phenotype, enhanceable by ed[+]/ed1X5

EgfrE3/EgfrE1 has wing phenotype, enhanceable by aosW11

EgfrE3/EgfrE1 has wing vein | ectopic phenotype, enhanceable by aosW11

EgfrE3 has wing vein phenotype, enhanceable by ed1X5

EgfrE3 has wing vein | ectopic phenotype, enhanceable by rlSem

EgfrE3 has eye phenotype, enhanceable by rl[+]/rlSem

Suppressed by
Statement
Reference

EgfrE3 has eye phenotype, suppressible by brmK804R.Tag:HA/brm[+]

EgfrE3 has eye phenotype, suppressible by brm[+]/brm2

EgfrE3 has crossvein | supernumerary phenotype, suppressible by Pcyt116919/Cct1[+]

EgfrE3 has eye photoreceptor cell phenotype, suppressible by Pcyt116919/Cct1[+]

EgfrE3 has crossvein | supernumerary phenotype, suppressible by Cct1[+]/Scer\GAL4[-]/Pcyt1EP831

EgfrE3 has eye phenotype, suppressible by kay2

EgfrE3 has wing | ectopic phenotype, suppressible by kay2

EgfrE3 has wing | ectopic phenotype, suppressible by kay1

EgfrE3 has wing vein | ectopic phenotype, suppressible by Sps1k11320

EgfrE3 has eye phenotype, suppressible by ShcBG/Shc[+]

EgfrE3 has wing phenotype, suppressible by ShcBG/Shc[+]

EgfrE3 has eye phenotype, suppressible by Shc111-40/Shc[+]

EgfrE3 has wing phenotype, suppressible by Shc111-40/Shc[+]

EgfrE3 has wing vein phenotype, suppressible by emcD/emcD

EgfrE3/EgfrE1 has eye phenotype, suppressible by Nl1N-ts1

EgfrE3/EgfrE1 has eye phenotype, suppressible by aosW11

EgfrE3 has wing phenotype, suppressible by Jra1

EgfrE3 has wing phenotype, suppressible by JraIA109

EgfrE3 has phenotype, suppressible by spi1

EgfrE3 has phenotype, suppressible by dacP

EgfrE3 has phenotype, suppressible by H2/H[+]

EgfrE3 has phenotype, suppressible by Vnounspecified/Vno[+]

NOT suppressed by
Statement
Reference

EgfrE3 has eye disc | larval stage phenotype, non-suppressible by Hsap\CDKN1AGMR.PH

EgfrE3 has eye phenotype, non-suppressible by kayhs.PZ/kay2

EgfrE3 has wing | ectopic phenotype, non-suppressible by kayhs.PZ/kay2

Enhancer of
Statement
Reference

EgfrE3/Egfr[+] is an enhancer of wing phenotype of ash2S112411

EgfrE3/Egfr[+] is an enhancer of wing vein | ectopic phenotype of ash2S112411

EgfrE3/Egfr[+] is an enhancer of wing vein | supernumerary phenotype of Mkp31/Mkp32, Scer\GAL4[-]

EgfrE3/Egfr[+] is an enhancer of wing phenotype of N55e11

NOT Enhancer of
Statement
Reference

EgfrE3/Egfr[+] is a non-enhancer of axon & ocellus sensory structure phenotype of Scer\GAL4sca-537.4, htlDN.UAS.cMb

EgfrE3 is a non-enhancer of wing disc phenotype of ftk07918

Suppressor of
Statement
Reference

EgfrE3/Egfr[+] is a suppressor of eye phenotype of CycEJP

EgfrE3 is a suppressor of axon & ocellus sensory structure | conditional ts phenotype of Nrgl10

EgfrE3 is a suppressor of axon & mechanosensory neuron & adult head | conditional ts phenotype of Nrgl10

EgfrE3 is a suppressor of phenotype of vnddd-13

EgfrE3 is a suppressor of phenotype of vnL6

EgfrE3/Egfr[+] is a suppressor of phenotype of Vnounspecified

EgfrE3/Egfr[+] is a suppressor of phenotype of H2

EgfrE3/Egfr[+] is a suppressor of phenotype of kniunspecified

NOT Suppressor of
Statement
Reference

EgfrE3/Egfr[+] is a non-suppressor of axon & mechanosensory neuron & adult head phenotype of Scer\GAL4sca-537.4, htlDN.UAS.cMb

EgfrE3 is a non-suppressor of wing disc phenotype of ftk07918

Other
Additional Comments
Genetic Interactions
Statement
Reference

The small rough eye phenotype of EgfrE3/+ is suppressed by expression of the brmK804R.T:Ivir\HA1 genomic construct.

The small rough eye phenotype of EgfrE3/+ is suppressed by brm2/+.

geminink03202b/+ enhances the EgfrE3/+ small rough eye phenotype.

EgfrE3/+ adults have extra vein tissue at the distal end of L2. This phenotype is enhanced by ash2S112411/ash2S112411 - the resulting wings are more reduced and have more ectopic veination than ash2S112411/ash2S112411 animals.

EgfrE3/+, Cct116919/+ double mutants show reduced loss of photoreceptors in the eye compared to EgfrE3/+ single mutants. Additionally, the eyes of the double mutants show a more regular arrangement of ommatidia. The extra crossvein phenotype seen in the wings of EgfrE3/+ flies is partially suppressed in Cct1EP831/+, EgfrE3/+ double mutants, with the majority of these flies exhibiting two, rather than several, extra crossveins. This phenotype is further suppressed in Cct116919/+, EgfrE3/+ double mutants, with the majority of these flies exhibiting no, or only one, extra crossvein in the wing. Further, the expression of Cct1Ala.Scer\UAS under the control of Scer\GAL4sd-SG29.1 also partially suppresses the EgfrE3 wing phenotype. The EgfrE3 allele dominantly enhances the notched wing phenotype seen in N55e11/+ flies.

The number of ommatidia in the eyes of EgfrE1/EgfrE3 adults is not altered if the flies are also expressing BacA\p35GMR.PH, but the number of pigment cells is increased in these animals.

The addition of SelDk11320/+ to EgfrE3/+ animals leads to a complete suppression of the ectopic wing phenotype in 66% of wings and a partial suppression in the rest.

Heterozygous mutants do not cause any defects in the ocellar sensory system (OSS) neurogenesis or axon guidance.

The eye and wing phenotypes are dominantly suppressed by ShcBG or Shc111-40. The EgfrE3 phenotype is completely suppressed if the flies are also homozygous for ShcBG or Shc111-40 and the viability of these flies is restored.

Null alleles of ru interact dominantly with EgfrE3.

The ectopic wing vein phenotype of EgfrE3/+ flies is slightly enhanced by emc1/emc11 and slightly suppressed by emcD/emcD.

argosW11 enhances the extra wing vein phenotype of EgfrE1/EgfrE3 transheterozygotes but suppresses the eye phenotype. Nl1N-ts1 also suppresses the eye phenotype of EgfrE1/EgfrE3.

Appearance of ectopic wing tissue in the adult wing is suppressed by loss of a functional copy of Jra (Jra1 or JraIA109).

25 minutes of heat induced stghs.PE3 expression causes mitotic cell appearance in the eye and wing discs. Induction of mitosis in the eye discs leads to a marked reduction of pyknotic bodies that were normally present on the basal side of the discs. Cellular progression into and through apoptosis is disrupted by ectopic stg expression. Heat induced stg expression does not affect the mutant eye phenotype: adults exhibit small elliptical and rough eyes as seen in EgfrE3 homozygotes.

Suppresses the viability of vnddd-13, the proliferation defects in vnL6 and (partially) the small disc phenotype of vnγ4.

The heterozygous extra wing vein phenotype is enhanced when transheterozygous with rlSem. Flies heterozygous for EgfrE3 and rlSem have smaller eyes than EgfrE3 heterozygotes alone.

Reduction of the dose of spi causes some suppression of the EgfrE3 phenotype; more ommatidia and increase in the size of the eye. S218 EgfrE3 double heterozygous adults exhibit severely reduced and rough eyes. Most ommatidia have a reduced number of photoreceptor cells, there are also defects in orientation, spacing and pigment cell and bristle numbers. This phenotype is more severe than a simple addition of the dominant effects of Egfr and S. Mutation has no effect on rhohs.sev rough eye phenotype.

Enhances the wing phenotype of Df(1)N-54l9, N264-39, Dl9P, and DlKX6 mutations, and the eye phenotype of Nspl-1.

Double mutant combinations of EgfrE3/EgfrE3 with netunspecified/netunspecified or px1/px1 have a superadditive phenotype. EgfrE3/EgfrE3 shows a negative interaction in combination with either rhounspecified/rhounspecified or kniunspecified/kniunspecified. EgfrE3/+ shows a negative interaction in combination with either H2/+ or Vnounspecified/+. EgfrE3/EgfrE3 shows a simple additive phenotype with tt1/tt1 and NAx-M1/+. EgfrE3/+ shows a simple additive phenotype with N55e11/+ and DlM1/+. E(spl)rv1 grounspecified EgfrE3/EgfrE3 flies show a simple additive phenotype. The lack of vein phenotype of rhounspecified vnunspecified flies is partially rescued by EgfrE3/EgfrE3.

Xenogenetic Interactions
Statement
Reference

Expression of Hsap\CDKN1AGMR.PH in homozygous EgfrE3 mutants does not prevent the apoptosis of undifferentiated cells in the eye disc that is seen in the EgfrE3 mutant background.

Complementation and Rescue Data
Comments

Expression of Egfr1.Scer\UAS under the control of Scer\GAL4GMR.PF in EgfrE3 flies results in mitosis in all cells in the eye disc. Expression of stghs.PE3 using heat shock in EgfrE3 discs causes all G2-arrested cells to enter mitosis. This mitosis is abnormal, with the cells remaining in mitosis for 4 hours (mitosis normally lasts about 15 minutes). Extensive cell death occurs amongst the mitotically arrested cells from 2-4 hours, so that some of the disc epithelium is lost.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Clonal analysis shows that normal ommatidia are less likely to develop if precluster cells R2, R3, R4, R5 or R8 are EgfrE3/EgfrE3.

Egfr protein expression in the eye imaginal discs of EgfrE3 homozygous third instar larvae has been compared with wild-type expression. The pattern of cell divisions posterior to the morphogenetic furrow is altered in EgfrE3 homozygotes.

No interaction with P{sev-svp1} or P{sev-svp2} exists.

External Crossreferences and Linkouts ( 5 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
Synonyms and Secondary IDs (17)
References (54)