W15R | Egfr-PA
A887T | Egfr-PA; A936T | Egfr-PB
L24V | Egfr-PA
Amino acid replacement: W15R. Amino acid replacement: L24V. Amino acid replacement: A887T. Figure 1 and the text concerning this allele are at odds - the text is correct (note from N. Baker).
EgfrE3/+ adults have extra vein tissue at the distal end of L2. This phenotype is enhanced by ash2S112411/ash2S112411 - the resulting wings are more reduced and have more ectopic veination than ash2S112411/ash2S112411 animals.
EgfrE3/+, Cct116919/+ double mutants show reduced loss of photoreceptors in the eye compared to EgfrE3/+ single mutants. Additionally, the eyes of the double mutants show a more regular arrangement of ommatidia. The extra crossvein phenotype seen in the wings of EgfrE3/+ flies is partially suppressed in Cct1EP831/+, EgfrE3/+ double mutants, with the majority of these flies exhibiting two, rather than several, extra crossveins. This phenotype is further suppressed in Cct116919/+, EgfrE3/+ double mutants, with the majority of these flies exhibiting no, or only one, extra crossvein in the wing. Further, the expression of Cct1Ala.Scer\UAS under the control of Scer\GAL4sd-SG29.1 also partially suppresses the EgfrE3 wing phenotype. The EgfrE3 allele dominantly enhances the notched wing phenotype seen in N55e11/+ flies.
Heterozygous mutants do not cause any defects in the ocellar sensory system (OSS) neurogenesis or axon guidance.
The eye and wing phenotypes are dominantly suppressed by ShcBG or Shc111-40. The EgfrE3 phenotype is completely suppressed if the flies are also homozygous for ShcBG or Shc111-40 and the viability of these flies is restored.
25 minutes of heat induced stghs.PE3 expression causes mitotic cell appearance in the eye and wing discs. Induction of mitosis in the eye discs leads to a marked reduction of pyknotic bodies that were normally present on the basal side of the discs. Cellular progression into and through apoptosis is disrupted by ectopic stg expression. Heat induced stg expression does not affect the mutant eye phenotype: adults exhibit small elliptical and rough eyes as seen in EgfrE3 homozygotes.
Reduction of the dose of spi causes some suppression of the EgfrE3 phenotype; more ommatidia and increase in the size of the eye. S218 EgfrE3 double heterozygous adults exhibit severely reduced and rough eyes. Most ommatidia have a reduced number of photoreceptor cells, there are also defects in orientation, spacing and pigment cell and bristle numbers. This phenotype is more severe than a simple addition of the dominant effects of Egfr and S. Mutation has no effect on rhohs.sev rough eye phenotype.
Double mutant combinations of EgfrE3/EgfrE3 with netunspecified/netunspecified or px1/px1 have a superadditive phenotype. EgfrE3/EgfrE3 shows a negative interaction in combination with either rhounspecified/rhounspecified or kniunspecified/kniunspecified. EgfrE3/+ shows a negative interaction in combination with either H2/+ or Vnounspecified/+. EgfrE3/EgfrE3 shows a simple additive phenotype with tt1/tt1 and NAx-M1/+. EgfrE3/+ shows a simple additive phenotype with N55e11/+ and DlM1/+. E(spl)rv1 grounspecified EgfrE3/EgfrE3 flies show a simple additive phenotype. The lack of vein phenotype of rhounspecified vnunspecified flies is partially rescued by EgfrE3/EgfrE3.
Expression of Egfr1.Scer\UAS under the control of Scer\GAL4GMR.PF in EgfrE3 flies results in mitosis in all cells in the eye disc. Expression of stghs.PE3 using heat shock in EgfrE3 discs causes all G2-arrested cells to enter mitosis. This mitosis is abnormal, with the cells remaining in mitosis for 4 hours (mitosis normally lasts about 15 minutes). Extensive cell death occurs amongst the mitotically arrested cells from 2-4 hours, so that some of the disc epithelium is lost.