Allele Dmel\Egfr^f1

General Information
Symbol	Dmel\Egfr^f1	Species	D. melanogaster
Name		FlyBase ID	FBal0003529
Feature type	allele	Associated gene	Dmel\Egfr
Also Known As	flb^1F26, flb^IF26, Egfr^1F26, Egfr^ts

Map ( GBrowse )
Allele class	temperature conditional amorphic allele - genetic evidence, heat sensitive loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	heat sensitive loss of function allele (Clifford and Schupbach, 1992) temperature conditional amorphic allele - genetic evidence (Raz and Shilo, 1992)
Mutagen	ethyl methanesulfonate (Tearle and Nusslein-Volhard, 1987, Clifford and Schupbach, 1989, Clifford and Schupbach, 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:17,445,791..17,445,791 evidence=experimental na_change=C17445791T pr_change=P1113L\|Egfr-PA,P1162L\|Egfr-PB reported_na_change=C3844T reported_pr_change=P1113L (Raz et al., 1991, Clifford and Schupbach, 1994)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name X78920 CAA55523

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: C3844T. Amino acid replacement: P1113L. (Clifford and Schupbach, 1994) Amino acid replacement: P1112L. (Raz and Shilo, 1992) Nucleotide substitution: C3335T. Amino acid replacement: P1112L. (numbering relates to the type II 5' splicing alternative, FBrf0043895). This amino acid residue is in the tyrosine kinase domain. (Raz et al., 1991)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| embryonic stage (Lanoue and Jacobs, 1999, Baker and Rubin, 1992) lethal \| embryonic stage \| heat sensitive (Raz and Shilo, 1993, Clifford and Schupbach, 1992, Yarnitzky et al., 1997) lethal \| embryonic stage \| recessive (Schejter and Shilo, 1989, Clifford and Schupbach, 1989) lethal \| embryonic stage \| recessive \| heat sensitive (Clifford and Schupbach, 1994) lethal \| heat sensitive (Szuts et al., 1998) visible \| heat sensitive (Guichard et al., 1999)
Phenotype Manifest In
	abdominal 1 ventral denticle belt (Raz and Shilo, 1993) abdominal 2 dorsal acute muscle 1 (Buff et al., 1998) abdominal 2 lateral longitudinal muscle 1 (Buff et al., 1998) abdominal 2 ventral acute muscle 2 (Buff et al., 1998) abdominal 2 ventral denticle belt (Raz and Shilo, 1993) abdominal 3 dorsal acute muscle 1 (Buff et al., 1998) abdominal 3 lateral longitudinal muscle 1 (Buff et al., 1998) abdominal 3 ventral acute muscle 2 (Buff et al., 1998) abdominal 3 ventral denticle belt (Raz and Shilo, 1993) abdominal 4 dorsal acute muscle 1 (Buff et al., 1998) abdominal 4 lateral longitudinal muscle 1 (Buff et al., 1998) abdominal 4 ventral acute muscle 2 (Buff et al., 1998) abdominal 4 ventral denticle belt (Raz and Shilo, 1993) abdominal 5 dorsal acute muscle 1 (Buff et al., 1998) abdominal 5 lateral longitudinal muscle 1 (Buff et al., 1998) abdominal 5 ventral acute muscle 2 (Buff et al., 1998) abdominal 5 ventral denticle belt (Raz and Shilo, 1993) abdominal 6 dorsal acute muscle 1 (Buff et al., 1998) abdominal 6 lateral longitudinal muscle 1 (Buff et al., 1998) abdominal 6 ventral acute muscle 2 (Buff et al., 1998) abdominal 6 ventral denticle belt (Raz and Shilo, 1993) abdominal 7 dorsal acute muscle 1 (Buff et al., 1998) abdominal 7 lateral longitudinal muscle 1 (Buff et al., 1998) abdominal 7 ventral acute muscle 2 (Buff et al., 1998) abdominal 7 ventral denticle belt (Raz and Shilo, 1993) Bolwig organ \| heat sensitive (Dumstrei et al., 2002) circumesophageal tract \| precursor \| heat sensitive (Page, 2003) commissure (Raz and Shilo, 1992) denticle belt (Szuts et al., 1997, Rogge et al., 1995) embryo (Lanoue and Jacobs, 1999) embryonic/larval cuticle (Szuts et al., 1997) embryonic/larval midgut \| embryonic stage (Szuts et al., 1998) embryonic/larval muscle system (Yarnitzky et al., 1997) embryonic/larval somatic muscle (Buff et al., 1998) embryonic central brain \| heat sensitive (Page, 2003) embryonic head \| heat sensitive (Dumstrei et al., 2002) embryonic neuroblast (Yagi et al., 1998) embryonic optic lobe \| heat sensitive (Dumstrei et al., 2002) embryonic optic lobe primordium \| heat sensitive (Dumstrei et al., 2002) endoderm (Szuts et al., 1998) epidermis (Raz and Shilo, 1992) extended germ band embryo (Raz and Shilo, 1992) eye (Kurada and White, 1998) genital disc primordium (Chen et al., 2005) head (Raz and Shilo, 1992) Keilin's organ (Raz and Shilo, 1993) longitudinal connective (Raz and Shilo, 1992) midgut constriction 1 (Szuts et al., 1998) midgut constriction 2 (Szuts et al., 1998) midgut constriction 3 (Szuts et al., 1998) midline glial cell (Raz and Shilo, 1992) RP2 motor neuron (Yagi et al., 1998) ventral thoracic disc primordium (Kubota et al., 2000) wing vein L4 \| heat sensitive (Guichard et al., 1999)
Detailed Description
	Statement Reference In Egfr^f1 stage 15 embryos, the genital disc precursor cells are completely missing. (Chen et al., 2005) When mutants are raised to the non-permissive temperature at ES 9, then a strong loss of midbrain phenotype is seen. When Egfr is removed at late ES 12, there is no gross loss of midbrain, however the circumesophageal connectives are sometimes disrupted. (Page, 2003) Heat pulses between 3 and 6 hours of development in Egfr^f1 embryos result in defects in head development, but leave the visual system intact. Heat pulses at 6-8 hours of development result in non-disjunction of the Bolwig's organ and optic lobe. Heat pulses from 8-10 hours of development do not affect optic placode morphogenesis but result in a reduction in Bolwig's neurons. (Dumstrei et al., 2002) Leg disc development is severely affected when the temperature is increased to the restrictive temperature at 6 hours after egg laying (AEL). Only a mild defect is found in leg discs when embryos are shifted to the restrictive temperature at 7 hours AEL. (Kubota et al., 2000) When Egfr^f1/Egfr^t1 flies are raised at the non-permissive temperature (29^oC)a vein-loss phenotype is seen. There is a large truncation in wing vein L4 which is similar to the Egfr^t1/Deficiency phenotype. This phenotype is seen if heat shocking occurs from 6 to 18 hours APF (after puparium formation). (Guichard et al., 1999) Egfr^f1 embryos grown at 25^oC generates a hypomorphic, embryonic lethal phenotype. (Lanoue and Jacobs, 1999) Embryos shifted to 29^oC at 6-7 hours of development fail to develop the LL1 muscle precursor. Embryos shifted to 29^oC at 5-6 hours of development fail to develop the LL1, DA1 and VA2 muscle precursors. (Buff et al., 1998) Shifting mutant embryos to the restrictive temperature after germ band retraction causes abnormal midgut development. (Szuts et al., 1998) sna-positive neuroblasts are often missing in homozygous embryos. Loss of RP2 motor neurons is also seen. (Yagi et al., 1998) Homozygous embryos shifted to the restrictive temperature (25^oC) at 6 hours of development give rise to larvae with residual denticle belts in their abdomens, often with 2-4 disordered rows of tapered denticles per belt. The regions of naked cuticle in between the denticle belts tend to be wider than in wild-type embryos. (Szuts et al., 1997) Embryonic muscle pattern is severely disrupted. (Yarnitzky et al., 1997) Homozygotes exhibit complete absence of ventral denticle belts. (Rogge et al., 1995) Homozygotes show a severe embryonic lethal phenotype at 29^oC and a weak embryonic lethal phenotype at 18^oC. Enhances the female sterility and adult morphological defects of Egfr^t1. Rarely survives as transheterozygote with the semi-viable Egfr^top-CA allele. (Clifford and Schupbach, 1994) Embryos shifted from the restrictive temperature to the permissive temperature after 5 hours of development display defective germband retraction and defective dorsal/ventral patterning. The cell fate of ventral cells is shifted to lateral cells. (Raz and Shilo, 1993) Partially suppresses the "Ellipse" phenotype at both restrictive and permissive temperatures. (Baker and Rubin, 1992) Homozygotes show a weak zygotic embryonic lethal phenotype at the permissive temperature (18^oC). They show a moderate or severe embryonic lethal phenotype, with all denticles being reduced in size at the restrictive temperature (29^oC). (Clifford and Schupbach, 1992) At the permissive temperature, homozygous Egfr^f1 embryos do not hatch, but the cuticle and CNS is similar to wild-type. At the restrictive temperature the cuticle and CNS show a severe phenotype, comparable to null Egfr alleles: the germ band does not retract, and the longitudinal axon tracts of the CNS are disarrayed. Temperature shifts at different times show that the phenotype can be dissected into at least five temporally and spatially distinct components, which affect many processes, including germ band retraction, CNS development, differentiation of cuticle secreting epidermal cells, differentiation of head structures, and differentiation and survival of midline glial cells. (Raz and Shilo, 1992) Homozygotes and hemizygotes display a weak 'flb' phenotype. Embryos produced from heteroallelic combination with Egfr^t1 have a severe ventralised phenotype, reduction in size of their dorsal appendage. (Clifford and Schupbach, 1989) Weak lethal phenotype: embryos lack some head and telson structures and have some development of the ventral cuticle. (Schejter and Shilo, 1989) weak allele
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Egfr[+]/Egfr^f1 is an enhancer of visible \| heat sensitive phenotype of peb¹ (Wilk et al., 2004) Egfr^f1 is an enhancer of visible phenotype of gro¹ (Price et al., 1997)
Phenotype Manifest In
Enhanced by
Statement Reference Egfr^f1, S^IIN has somatic muscle \| embryonic stage phenotype, enhanceable by spi¹ (Buff et al., 1998) Egfr^f1, spi¹ has somatic muscle \| embryonic stage phenotype, enhanceable by S[+]/S^IIN (Buff et al., 1998) Egfr^f1 has somatic muscle \| embryonic stage phenotype, enhanceable by S^IIN (Buff et al., 1998)
Suppressed by
Statement Reference Egfr^f1 has denticle belt phenotype, suppressible \| partially by aop^e2d (Rogge et al., 1995)
Enhancer of
Statement Reference Egfr[+]/Egfr^f1 is an enhancer of eye \| heat sensitive phenotype of peb¹ (Wilk et al., 2004) Egfr^f1 is an enhancer of eye phenotype of gro¹ (Price et al., 1997) Egfr^f1 is an enhancer of eye phenotype of W^GMR.PG/W^GMR.PG (Kurada and White, 1998) Egfr^f1 is an enhancer of interommatidial bristle phenotype of gro¹ (Price et al., 1997) Egfr^f1 is an enhancer of ommatidium phenotype of gro¹ (Price et al., 1997) Egfr^f1 is an enhancer of phenotype of csw^lf (Firth et al., 2000) Egfr^f1 is an enhancer of prothoracic leg phenotype of gro¹ (Price et al., 1997) Egfr^t1/Egfr^f1 is an enhancer of wing vein phenotype of rho^ve-1 (Guichard et al., 1999)
Suppressor of
Statement Reference Egfr^f1 is a suppressor of phenotype of rho^hs.sev (Freeman, 1994)
NOT Suppressor of
Statement Reference Egfr^f1 is a non-suppressor of denticle phenotype of wg^l-17 (Szuts et al., 1997)
Additional Comments
Genetic Interactions
Statement Reference When Egfr^f1/Egfr^t1; rho^ve-1/rho^ve-1 flies are raised at the permissive temperature (18^oC) a wing vein phenotype is seen that is similar to (but slightly more severe than) rho^ve-1/rho^ve-1 alone; wing veins L4 and L5 have prominent distal truncations, L2 and L3 remain largely intact. When raised at the non-permissive temperature (29^oC) there is an enhancement of the wing vein phenotype with all wing veins showing major truncations. This phenotype is seen if heat shocking occurs from 0 to 24 hours APF (after puparium formation). (Guichard et al., 1999) Embryos raised at 18^oC have a mild muscle phenotype, which is dominantly enhanced by S^IIN. The mild Egfr^f1 muscle phenotype is not affected by one copy of spi¹ alone, but spi¹ shows additional enhancement of the muscle phenotype in combination with S^IIN. (Buff et al., 1998) Enhances the eye reduction phenotype caused by W^GMR.PG. (Kurada and White, 1998) Egfr^f1; gro¹/gro¹ flies display an enhanced gro phenotype: ectopic compound eyes, fused or bifurcated legs. These phenotypes are almost exclusively restricted to males. The ectopic eyes are organised into arrays of ommatidia. They are frequently adjacent to the endogenous eye but separated by a discrete border and exhibit defects in ommatidial packing and the distribution of interommatidial bristles. (Price et al., 1997) Does not alter the denticle phenotype of wg^l-17 larvae. (Szuts et al., 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer
	Selected as: Embryonic lethal.
Comments
	Class I allele. (Clifford and Schupbach, 1994) Temperature shift analysis identifies two distinct times at which Egfr is required for embryonic development. Germline clone analysis indicates that there is very little, if any, requirement for Egfr in the germline. (Clifford and Schupbach, 1992) Weak allele. (Schejter and Shilo, 1989)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 18 )
Reported As
Symbol Synonym	egfr¹ (Dubois et al., 2001) Egfr^1F26 (Firth et al., 2000, Yarnitzky et al., 1997) Egfr^1f26 (Guichard et al., 1999) EgfR^f1 (Wilk et al., 2004) Egfr^f1 (Jiang and Edgar, 2009) Egfr^f (Rogge et al., 1995) Egfr^top-1F26kb Egfr^ts (Brennan and Moses, 2000, Guichard et al., 1999) flb flb^1F26 (Szuts et al., 1998, Buff et al., 1998, Golembo et al., 1996, Gabay et al., 1996, Golembo et al., 1996, Schweitzer et al., 1995, Raz and Shilo, 1993, Raz and Shilo, 1992, Baker and Rubin, 1992, Shilo and Raz, 1991, Raz et al., 1991, Schejter and Shilo, 1989, ) flb^IF26 (Chen et al., 2005, Tian et al., 2004, Lanoue and Jacobs, 1999, Kurada and White, 1998, Yagi et al., 1998, Szuts et al., 1997, Yagi and Hayashi, 1997, Rogge et al., 1995) flb^IF (Tearle and Nusslein-Volhard, 1987) flb^IIF26 (Lanoue and Jacobs, 1999) top^1F26 (Clifford and Schupbach, 1994) top^1F26kb (Clifford and Schupbach, 1989) top^1F (Roth and Schupbach, 1994) top^IF26 (Clifford and Schupbach, 1992)
Name Synonym	faint little ball
Secondary FlyBase IDs
	FBal0033663
References ( 40 )

Generate a list of	All references relating to this allele ( 40 )

List References by type	Research paper ( 36 ) Review ( 3 ) Stock list ( 1 )
Recent research papers (0)
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Allele Dmel\Egfrf1

Allele Dmel\Egfr^f1