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General Information
Symbol
Dmel\Egfrf7
Species
D. melanogaster
Name
FlyBase ID
FBal0003535
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
flb2W74, top2W74
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C21558205T

Reported nucleotide change:

C3763T

Amino acid change:

T1086I | Egfr-PA; T1135I | Egfr-PB

Reported amino acid change:

T1086I

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C3763T. Amino acid replacement: T1086I.

Single amino acid substitution in the kinase domain.

Nucleotide substitution: C3253T. Amino acid replacement: T1087I. This threonine residue in the tyrosine kinase domain is conserved in all members of the EGF receptor class.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

microchaeta & abdominal sternite

microchaeta & abdominal tergite

Detailed Description
Statement
Reference

In the temperature sensitive combination Egfrtop-CA/Egfrf7 the pleura expands at the expense of the sternites and tergites.

All RP2 and RP2sib neurons are missing.

At 25oC, approximately 90% of embryos fail to undergo head involution. Development of the cuticle and central nervous system and germband retraction are also abnormal. At 28oC, homozygous embryos have a "faint little ball" phenotype with poor cuticle formation.

Mutant embryos show absence of dorsal trunk and reduced visceral branches. Dorsal branch and lateral trunks are normal. The dorsal trunk is rescued in a put135 mutant background.

25-49% of Egfrtop-CA/Egfrf7 flies survive to adulthood (FBrf0049928). The anterior dorsal, posterior dorsal, ventral and spiracular histoblast nests contain fewer cells than in wild-type pupae 6 hours after pupariation. The dorsal histoblast nests remain separate 24 hours after pupariation, in contrast to wild-type where they are fused. The histoblast nests in the anterior segments are less abnormal than those in posterior segments. Adults sometimes have holes in the abdominal cuticle and lack spiracles in the abdominal segments. In less severely affected adults chaetae appear abnormal and the distribution of hairs on the tergite, sternite and pleura is irregular and sparse. The thickness of the cuticle is reduced and spiracles are abnormal.

Homozygotes show a severe embryonic lethal phenotype at 29oC and a weak embryonic lethal phenotype at 18oC. Enhances the female sterility and adult morphological defects of Egfrt1. Rarely survives as transheterozygote with the semi-viable Egfrtop-CA allele.

At 29oC Malpighian tubules in homozygous embryos exhibit a very reduced number of cells. Numbers are close to those of wild type when raised at 18oC. Temperature shift experiments reveal Egfr is required for normal tubule development from 3.25 hours AEL until after 8 hours AEL.

Homozygotes and hemizygotes display a weak 'flb' phenotype. Embryos produced from heteroallelic combination with Egfrt1 have a severe ventralised phenotype, reduction in size of their dorsal appendage.

Weak lethal phenotype: embryos lack some head and telson structures and have some development of the ventral cuticle.

weak allele

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement
Reference

Egfrf7 has head phenotype, suppressible by Dsor1Su1

Egfrf7 has head phenotype, suppressible by Dsor1Su4

Suppressor of
Statement
Reference

Egfrf7 is a suppressor of phenotype of rhohs.sev

Additional Comments
Genetic Interactions
Statement
Reference

Failure to undergo head involution at 25oC is partly suppressed if both parents are hemi- or homozygous for either Dsor1Su1 or Dsor1Su4. Lack of cuticle formation at 28oC is partly suppressed by maternal expression of Dsor1Su1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Selected as: Embryonic lethal.

Comments
Comments

Mutation of Egfr that coordinately affects all gene activities, a class I lesion. The allelic series for class I lesions: Egfrt2 = Egfrt1 < Egfrtop-EC20 < Egfrf7 = Egfrf1 = Egfrflb-2E07 < Egfrtop-EE39 = Egfrtop-ED26 = Egfrf5 < Egfrf9 = Egfrf10 = Egfrf2 = Egfrtop-EE42 = Egfrf11 = Egfrf24 = Egfrf3 = Df(2R)Egfr3.

Less than 10% of wild type number of ventral epidermal cells expressing P{lacZ}BP28 are evident in mutant embryos. oc expression is greatly reduced along the midline.

Weak mutation.

External Crossreferences and Linkouts ( 2 )
Crossreferences
GenBank Nucleotide - A collection of sequences from several sources, including GenBank, RefSeq, TPA, and PDB.
GenBank Protein - A collection of sequences from several sources, including translations from annotated coding regions in GenBank, RefSeq and TPA, as well as records from SwissProt, PIR, PRF, and PDB.
Synonyms and Secondary IDs (8)
References (16)