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General Information
Symbol
Dmel\Egfrt1
Species
D. melanogaster
Name
FlyBase ID
FBal0003572
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
top1, topQY1, EGFRtop1, egfrQY1, EGFRtop, DERtop1, top, topQY, Egfr1, Egfrtop-1
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C21554848T

Reported nucleotide change:

C406T

Amino acid change:

S58F | Egfr-PA; S107F | Egfr-PB

Reported amino acid change:

S58F

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C406T. Amino acid replacement: S58F.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous embryos show wound closure defects.

Egfrf11/Egfrt1 flies show mild planar cell polarity defects in the eye.

Egfrt1 mutant flies lay ventralised eggs with a central, very thin and short dorsal appendage, or very little dorsal appendage material.

Egfrt1/Egfrf24 animals show loss of the anterior crossvein (100% penetrance) and partial loss of vein L4 in the wing (169% penetrance).

In Egfrf11/Egfrt1 adults, wings lack the anterior cross-vein and a segment of L4.

Egfrt1/Df(2R)Egfr3 flies lack the anterior crossvein and the central portion of wing vein L4.

Mutant adults have rough eyes. A loss of photoreceptor cells is seen as well as many ommatidia with rotation defects. Egfrt1/Egfrtop-EC20 animals display rotation defects in adult ommatidia.

Eggs laid by Egfrt1 homozygous females are strongly ventralised.

In EgfrE3/Egfrt1 mutants, some flies exhibit an extra crossvein at the tip of L2 in the wing.

Homozygous females lay eggs with a ventralised eggshell and embryo.

A fraction of embryos derived from Egfrt1 females gastrulate with two ventral furrows. Cuticles show an expansion of ventral structures.

Egfrt1/Egfrf37 flies show loss of wing vein L4. Egfrt1 homozygotes do not have a wing vein phenotype. Homozygous Egfrt1 wings are 14-20% smaller than wild-type.

Egfrt1/Egfrf11 flies have a rough eye phenotype, a gap in wing vein L4 and the anterior crossvein is missing.

Egfrt1/Egfrf2 phenotypes are similar to ebiP7/ebiE90 and partial female sterility resulting from partially ventralized eggs, wing vein defects, short bristles and rough eyes.

Eggs derived from Egfrt1/Egfrflb-2E07 females are ventralised and have no or only one dorsal appendage.

When Egfrf1/Egfrt1 flies are raised at the non-permissive temperature (29oC)a vein-loss phenotype is seen. There is a large truncation in wing vein L4 which is similar to the Egfrt1/Deficiency phenotype. This phenotype is seen if heat shocking occurs from 6 to 18 hours APF (after puparium formation).

The notum is significantly reduced in size in Egfrtsla/Egfrt1 wing discs raised at the non-permissive temperature.

Eggs laid by Egfrt1 mothers are ventralised, with either a fused single dorsal appendage, or two appendages closer together than seen in wild-type.

Egfrf37/Egfrt1 lack wing vein L4.

Homozygous females or heterozygotes of Egfrt1 with Egfrf11 produce eggs with fused dorsal appendages. Larvae hatch from the eggs, with dorsal-ventral pattern unperturbed.

Females produce eggs with dorsally fused dorsal appendages.

Dorsal appendages are reduced in size and fused, and are shifted slightly posteriorly in Egfrt1/Df(2R)Pu-D17 eggs.

Dsor1 mutant alleles do not suppress the eggshell dorso-ventral polarity defect of the Egfrt1 maternal mutation.

Wings are often indistinguishable from wild-type but may show deletions of the anterior crossveins. Hemizygous flies display a more severe defect, typically missing a section of wing vein L4.

Transheterozygous females with Egfrf2, Egfrf3 or Egfrf8 display egg chambers with gaps in the follicular epithelium that uncover the nurse cells. Eggs derived from these females display weak or intermediate ventralised phenotypes.

Ventralised egg chambers. Egg chambers become elongated in shape, dorsal appendages fuse and shrink and the number of main body follicle cells increases. Overexpression of P{CF2-s} in heterozygotes generates more ventralised eggs.

Homozygotes have mutant egg chambers. Posterior follicle cells of Egfrtop-CA/Egfrt1 transheterozygotes have an abnormal shape and behaviour.

Most Egfrt1/Df(2R)Egfr18 heterozygous embryos have one fused dorsal appendage at the dorsal midline, few have a posterior micropyle and about half contain embryos.

Eggs laid by homozygous females are ventralised, fused and shortened dorsal appendages and the eggs are longer than wild type. Expression of phlF22.hs causes females to lay ventralised eggs, expression in the anterior follicle cells of homozygotes or heterozygotes with Egfrt2 causes dorsalisation of the egg chamber.

Weak and intermediate ventralised embryos.

Homozygous adult viable.

Clonal analysis reveals phenotypes in the adult including loss of wing vein, ectopic wing vein, reduced cell size, extra bristles, cell lethality and tergite bristle abnormalities. Phenotype is cell autonomous. rl1/Df(2R)rl10a strongly enhances the wing phenotype of Egfrt1/Egfrf37.

Weak ventralization of embryos. Filzkorper are normal. Only one dorsal appendage forms. 51% of laid eggs contain embryos. Mesoderm is wider than usual and cannot be enveloped by remaining epidermis. Two ventral furrows form.

Egfrt1 homozygous females also heterozygous or homozygous for fs(1)K1013 or fs(1)K101 produce eggs with a ventralised phenotype.

Embryos from double mutants with sqdix43 are strongly dorsalised.

Heterozygous rhove-1 dominantly enhances the homozygous phenotype. Heterozygous rhoMod suppresses the homozygous phenotype.

Does not suppress the "Ellipse" phenotype. Ommatidia are missing photoreceptor cells, and photoreceptor cell morphology may be abnormal in hemizygotes.

Reduction or absence of dorsal appendages (ventralization) with increase in main body egg shell. Micropyle at both ends of eggs, embryos ventralized.

An enlarged mass of mesodermal cells invaginates on the ventral side of the embryo, the enlarged mesoderm is often organised into two ventral furrows. Organisation is lost in later stages of development and a mass of mesodermal cells fills the ventral half of the embryo. Respiratory appendages are fused and inserted directly on the dorsal midline.

maternal-effect lethal

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

Egfrt1 has visible phenotype, enhanceable by E(Egfr)B56B56

Egfrt1 has visible phenotype, enhanceable by E(Egfr)C12C12

Egfrt1 has visible phenotype, enhanceable by E(Egfr)C22C22

Egfrt1 has visible phenotype, enhanceable by groC105

Egfrt1 has visible phenotype, enhanceable by vnC221

Egfrt1 has visible phenotype, enhanceable by HC24

Egfrt1 has visible phenotype, enhanceable by HC57

Egfrt1 has visible phenotype, enhanceable by HC73

Suppressed by
Statement
Reference

Egfrt1/Egfrf24 has visible phenotype, suppressible by kek1RA5/kek1RM2

Egfrt1/Egfrf11 has visible phenotype, suppressible by sty[+]/styS73

Egfrt1/Egfrf11 has visible phenotype, suppressible by sty[+]/styS88

Enhancer of
Statement
Reference

Egfr[+]/Egfrt1 is an enhancer of visible phenotype of CycEJP

Egfr[+]/Egfrt1 is an enhancer of visible phenotype of rgγ6

Egfrt1 is an enhancer of visible phenotype of gro1

Egfrt1 is an enhancer of visible phenotype of rl1

NOT Enhancer of
Statement
Reference

Egfr[+]/Egfrt1 is a non-enhancer of visible phenotype of Scer\GAL4en-e16E, kermitGS2053

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

Egfr[+]/Egfrt1 is a non-suppressor of visible phenotype of Scer\GAL4en-e16E, kermitGS2053

Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Egfrf3/Egfrt1 has follicle cell phenotype, enhanceable by da[+]/da2

Egfrt1 has phenotype, enhanceable by ebiunspecified/ebi[+]

Egfrt1 has wing vein L4 phenotype, enhanceable by HC21

Egfrt1 has wing vein L2 phenotype, enhanceable by E(Egfr)B56B56

Egfrt1 has wing vein L4 phenotype, enhanceable by E(Egfr)B56B56

Egfrt1 has wing vein L5 phenotype, enhanceable by E(Egfr)B56B56

Egfrt1 has wing vein L4 phenotype, enhanceable by E(Egfr)C12C12

Egfrt1 has wing vein L4 phenotype, enhanceable by E(Egfr)C22C22

Egfrt1 has wing vein L4 phenotype, enhanceable by groC105

Egfrt1 has anterior crossvein phenotype, enhanceable by groC105

Egfrt1 has wing vein L4 phenotype, enhanceable by vnC221

Egfrt1 has wing phenotype, enhanceable by HC24

Egfrt1 has wing phenotype, enhanceable by HC57

Egfrt1 has wing phenotype, enhanceable by HC73

Egfrt1/Egfrtop-CA has phenotype, enhanceable by rl10b

Egfrt1/Egfrf11 has phenotype, enhanceable by H2/H[+]

Egfrt1/Egfrf11 has phenotype, enhanceable by NAx-M1/N[+]

Egfrt1/Egfrf11 has phenotype, enhanceable by Vno1/Vno[+]

NOT Enhanced by
Statement
Reference
Suppressed by
Statement
Reference

Egfrt1/Egfrf11 has ommatidium phenotype, suppressible | partially by fz[+]/fz21

Egfrt1/Egfrf11 has ommatidium phenotype, suppressible | partially by fz23/fz[+]

Df(2R)Egfr3/Egfrt1 has eye phenotype, suppressible | partially by kek1RA5/kek1[+]

Egfrt1/Egfrf24 has eye phenotype, suppressible | partially by kek1RA5/kek1[+]

Egfrflb-2E07/Egfrt1 has eye phenotype, suppressible | partially by kek1RA5/kek1[+]

Egfrflb-2E07/Egfrt1 has eye phenotype, suppressible | partially by kek1[+]/kek1RM2

Egfrt1/Egfrf11 has anterior crossvein phenotype, suppressible | partially by ash2[+]/ash2I1

Egfrt1/Egfrf11 has wing vein L4 phenotype, suppressible | partially by ash2[+]/ash2I1

Egfrt1 has ventral furrow | ectopic phenotype, suppressible by spz[+]/spzunspecified

Egfrt1 has ventral furrow | supernumerary phenotype, suppressible by spz[+]/spzunspecified

Egfrt1/Egfrf11 has wing vein L4 phenotype, suppressible by sty[+]/styS73

Egfrt1/Egfrf11 has anterior crossvein phenotype, suppressible by sty[+]/styS73

Egfrt1/Egfrf11 has eye phenotype, suppressible by sty[+]/styS73

Egfrt1/Egfrf11 has wing vein L4 phenotype, suppressible by sty[+]/styS88

Egfrt1/Egfrf11 has anterior crossvein phenotype, suppressible by sty[+]/styS88

Egfrt1/Egfrf11 has eye phenotype, suppressible by sty[+]/styS88

Egfrt1 has phenotype, suppressible by bs03267

Egfrt1/Egfrf11 has phenotype, suppressible by DlM1/Dl[+]

NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference

Egfr[+]/Egfrt1 is an enhancer of eye phenotype of CycEJP

Egfrt1 is an enhancer of ommatidium phenotype of S48-5

Egfr[+]/Egfrt1 is an enhancer of egg phenotype of Scer\GAL4VP16.nos.UTR, kek1UASp.cGa

Egfr[+]/Egfrt1 is an enhancer of eye phenotype of rgγ6

Egfrt1/Egfrf1 is an enhancer of wing vein phenotype of rhove-1

Egfrt1 is an enhancer of ocellus phenotype of gro1

Egfrt1 is an enhancer of adult head & microchaeta phenotype of gro1

Egfrt1 is an enhancer of ocellus phenotype of rl1

Egfrt1 is an enhancer of adult head & microchaeta phenotype of rl1

Egfrt1 is an enhancer of wing phenotype of rl1

Egfrt1 is an enhancer of wing phenotype of gro1

Egfrt1 is an enhancer of prothoracic leg phenotype of rl1

Egfrt1 is an enhancer of prothoracic leg phenotype of gro1

NOT Enhancer of
Statement
Reference

Egfr[+]/Egfrt1 is a non-enhancer of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053

Egfr[+]/Egfrt1 is a non-enhancer of embryonic epidermis phenotype of cactE10

Egfrt1 is a non-enhancer of embryonic epidermis phenotype of fus1

Suppressor of
Statement
Reference

Egfr[+]/Egfrt1 is a suppressor of adult midgut phenotype of BursZ5569

Egfr[+]/Egfrt1 is a suppressor of intestinal stem cell | adult stage phenotype of BursZ5569

Egfr[+]/Egfrt1 is a suppressor of wing vein | ectopic phenotype of Scer\GAL4Tub.PU, cswN308D.UASp

Egfr[+]/Egfrt1 is a suppressor of wing phenotype of NrgGPI.UAS, Nrgl3, Scer\GAL4MS1075

Egfr[+]/Egfrt1 is a suppressor of wing phenotype of NrgGPI.UAS, Scer\GAL4MS1075

Egfrt1 is a suppressor of wing phenotype of NrgGPI.UAS, Scer\GAL4MS1075

Egfr[+]/Egfrt1 is a suppressor of wing phenotype of Nrg167.UAS, Nrgl3, Scer\GAL4MS1075

Egfr[+]/Egfrt1 is a suppressor of wing phenotype of Nrg167.UAS, Scer\GAL4MS1075

Egfrt1 is a suppressor of wing phenotype of Nrg167.UAS, Scer\GAL4MS1075

Egfrt1 is a suppressor | partially of ommatidium phenotype of aosrlt

Egfrt1 is a suppressor of phenotype of svp1.sev

Egfrt1 is a suppressor of phenotype of svp2.sev

Egfrt1/Egfrf11 is a suppressor of phenotype of kn1

NOT Suppressor of
Statement
Reference

Egfr[+]/Egfrt1 is a non-suppressor of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053

Egfrt1 is a non-suppressor of ommatidium phenotype of ecspok

Egfr[+]/Egfrt1 is a non-suppressor of egg chorion phenotype of Egfr::kek1KEΔCG.UAS.GFP, Scer\GAL4CY2

Other
Additional Comments
Genetic Interactions
Statement
Reference

Egfrt1/+ suppresses the intestinal stem cell hyperproliferation seen in BursZ5569/BursZ5569 mutants.

The presence of a Egfrt1 background significantly suppresses ectopic wing vein formation found in cswY279C.Scer\UAS (Scer\GAL4tub) mutants.

A Egfrt1 background suppresses the cswY279C.Scer\UAS (Scer\GAL4tub) rough eye phenotype, normalizing the numbers of R7 and ommatidial rotation.

The planar cell polarity defects seen in Egfrf11/Egfrt1 eyes are partially suppressed by fz21/+ or fz23/+.

The ommatidial rotation defects observed in ecspok hemizygous adult eyes are not suppressed by Egfrt1/+.

Heterozygous kek1RM2 or kek1RA5 partially suppresses the rough eye phenotype seen Egfrflb-2E07/Egfrt1 animals. kek1RM2/kek1RA5 fully suppresses the phenotype. Heterozygous kek1RA5 partially suppresses the rough eye phenotype seen Egfrf24/Egfrt1 animals. Heterozygous kek1RA5 partially suppresses the rough eye phenotype seen Df(2R)Egfr3/Egfrt1 animals.

The loss of wing vein L4 seen in Egfrt1/Egfrf24 animals is suppressed if they are also mutant for kek1 (kek1RA5/kek1RM2). The dorsalised chorion phenotype caused by expression of Egfr::kek1KEΔCG.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4CY2 is not suppressed if the flies are also heterozygous for Egfrt1.

The loss of anterior cross-vein and L4 gap seem in Egfrf11/Egfrt1 wings are partially rescue by ash2I1/+.

The defective wing phenotype, seen in both Nrg167.Scer\UAS and NrgGPI.Scer\UAS mutants expressed under the control of Scer\GAL4MS1075, is partially suppressed in a Egfrt1 or Egfrt1/+ background. Egfrt1 also suppresses the wing phenotype when either Nrg167.Scer\UAS or NrgGPI.Scer\UAS is expressed in a Nrgl3 background.

S48-5/Egfrt1 mutants show 41.52%+-7.24 misrotated ommatidia compared to 9.55%+-1.43 seen in S48-5 mutants alone.

The ventralized phenotype of eggs laid by Egfrt1 homozygous females is unaffected by Egfr::kek1Scer\UAS.cGa; Scer\GAL4T155. The penetrance and expressivity of the weak ventralization phenotype seen in eggs laid by kek1Scer\UAS.P\T.cGa; Scer\GAL4nos.UTR.T:Hsim\VP16 females is strongly enhanced by heterozygosity for Egfrt1 (64% of eggs are ventralized, 8% strongly ventralised).

The extra crossvein wing phenotype of Cct1Ala.Scer\UAS mutants is enhanced when Cct1Ala.Scer\UAS is overexpressed (driven by Scer\GAL4sd-SG29.1) in Egfrt1/+ flies.

Egfrt1/Egfrt1 ; cicfet-T6/cicfet-E11 females lay eggs with a dorsalised embryo (like cicfet-T6/cicfet-E11 single mutant females) and an eggshell that shows a combination of cic and Egfr mutant phenotypes; the dorsal appendages are broadened (as in cic mutants) but are positioned closer to the dorsal midline (as in Egfr mutants).

Embryos derived from Egfrt1 ; spzunspecified/+ females fail to invaginate ventral furrows and their cuticles show a reduction in ventral fates as compared to embryos laid by Egfrt1 single mutant females.

This loss of wing vein L4 seen in Egfrt1/Egfrf37 flies is suppressed by emc1/emc11. emcD enhances the loss of wing veins seen in Egfrt1/Egfrf37 flies. Egfrt1 emcD double homozygotes show loss of wing veins.

The Egfrt1/Egfrf11 phenotypes are dominantly suppressed by styS73 or styS88.

The Egfrt1/Egfrflb-2E07 phenotype is partially suppressed by kek1RA5/kek1RM2.

When Egfrf1/Egfrt1; rhove-1/rhove-1 flies are raised at the permissive temperature (18oC) a wing vein phenotype is seen that is similar to (but slightly more severe than) rhove-1/rhove-1 alone; wing veins L4 and L5 have prominent distal truncations, L2 and L3 remain largely intact. When raised at the non-permissive temperature (29oC) there is an enhancement of the wing vein phenotype with all wing veins showing major truncations. This phenotype is seen if heat shocking occurs from 0 to 24 hours APF (after puparium formation).

Egfrt1/Egfrt1; HC21/H+ flies exhibit reduced numbers of macrochaetae, most noticeably on the head. Egfrt1/Df(2R)Egfr18; gro1/gro1 flies display a combination of Egfr and gro phenotypes. The L4 wing vein defect is restored and the ocellar bristles are present but disarrayed. In many cases supernumerary ocellar bristles and/or interocellar bristles are present producing a patch of bristles where the ocelli would normally have been. The ocelli are either reduced or eliminated. Most males display one or more ectopic eye structure dorso-medial to the normal eyes. These structures are small and contain discrete ommatidia but no interommatidial bristles. The lens material of these ectopic eyes is fused into a uniform glaze and they are recessed into the head cuticle.

Weak suppression of the dosage-dependent (two or more copies of P{sev-svp1} or P{sev-svp2}) transformation of cone cells into R7 photoreceptors and at a lower frequency R7 cells into outer photoreceptors.

rl10b causes strong dominant enhancement of the Egfrtop-CA/Egfrt1 transheterozygous phenotype.

Double mutants of Df(2R)rl10a and Egfrt1 show disruption of the longitudinal vein L4.

dacP, Egfrt1 double homozygotes have a reduced, rough eye phenotype that exceeds that of either mutant alone or the expected additive effects of the two mutants.

Double mutant combinations of Egfrt1/Egfrf11 with rhounspecified/rhounspecified, vnunspecified/vnunspecified, ciunspecified/ciunspecified, astunspecified/astunspecified, tt1/tt1, Vnounspecified/+, H2/+, vvlunspecified/+ or NAx-M1/+ have a superadditive phenotype. Double mutant combinations of Egfrt1/Egfrf11 with kn1/kn1 or netunspecified/netunspecified show mutual suppression, with both the Egfr and vein phenotypes being normalised. Egfrt1/Egfrf11 shows a negative interaction in combination with either DlM1/+ or tkvunspecified/tkvunspecified. Egfrt1/Egfrf11 shows a simple additive phenotype with dppunspecified/dppunspecified, kniunspecified/kniunspecified, tg2/tg2, cv-2unspecified/cv-2unspecified and N55e11/+. E(spl)rv1 grounspecified Egfrt1/Egfrf11 flies show a simple additive phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Weak Egfr allele.

Mutation of Egfr that coordinately affects all gene activities, a class I lesion. The allelic series for class I lesions: Egfrt2 = Egfrt1 < Egfrtop-EC20 < Egfrf7 = Egfrf1 = Egfrflb-2E07 < Egfrtop-EE39 = Egfrtop-ED26 = Egfrf5 < Egfrf9 = Egfrf10 = Egfrf2 = Egfrtop-EE42 = Egfrf11 = Egfrf24 = Egfrf3 = Df(2R)Egfr3.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (21)
Reported As
Symbol Synonym
Egfrtop-QY1
Name Synonyms
Secondary FlyBase IDs
    References (86)