C21554848T
C406T
S58F | Egfr-PA; S107F | Egfr-PB
S58F
Nucleotide substitution: C406T. Amino acid replacement: S58F.
crossvein | increased number (with EgfrE3)
eye (with Df(2R)Egfr3)
eye (with Egfrflb-2E07)
follicle cell (with Egfrf3)
ommatidium (with Egfrf11)
wing vein L4 (with Egfrf11)
wing vein L4 (with Egfrf24)
Homozygous embryos show wound closure defects.
Egfrt1 mutant flies lay ventralised eggs with a central, very thin and short dorsal appendage, or very little dorsal appendage material.
Egfrt1/Egfrflb-2E07, Egfrt1/Egfrflb-2E07 and Egfrt1/Df(2R)Egfr3 animals have rough eyes.
Egfrt1/Df(2R)Egfr3 flies lack the anterior crossvein and the central portion of wing vein L4.
Mutant adults have rough eyes. A loss of photoreceptor cells is seen as well as many ommatidia with rotation defects. Egfrt1/Egfrtop-EC20 animals display rotation defects in adult ommatidia.
Eggs laid by Egfrt1 homozygous females are strongly ventralised.
Homozygous females lay eggs with a ventralised eggshell and embryo.
A fraction of embryos derived from Egfrt1 females gastrulate with two ventral furrows. Cuticles show an expansion of ventral structures.
Eggs derived from Egfrt1/Egfrflb-2E07 females are ventralised and have no or only one dorsal appendage.
When Egfrf1/Egfrt1 flies are raised at the non-permissive temperature (29oC)a vein-loss phenotype is seen. There is a large truncation in wing vein L4 which is similar to the Egfrt1/Deficiency phenotype. This phenotype is seen if heat shocking occurs from 6 to 18 hours APF (after puparium formation).
Eggs laid by Egfrt1 mothers are ventralised, with either a fused single dorsal appendage, or two appendages closer together than seen in wild-type.
Females produce eggs with dorsally fused dorsal appendages.
Dorsal appendages are reduced in size and fused, and are shifted slightly posteriorly in Egfrt1/Df(2R)Pu-D17 eggs.
Wings are often indistinguishable from wild-type but may show deletions of the anterior crossveins. Hemizygous flies display a more severe defect, typically missing a section of wing vein L4.
Ventralised egg chambers. Egg chambers become elongated in shape, dorsal appendages fuse and shrink and the number of main body follicle cells increases. Overexpression of P{CF2-s} in heterozygotes generates more ventralised eggs.
Homozygotes have mutant egg chambers. Posterior follicle cells of Egfrtop-CA/Egfrt1 transheterozygotes have an abnormal shape and behaviour.
Most Egfrt1/Df(2R)Egfr18 heterozygous embryos have one fused dorsal appendage at the dorsal midline, few have a posterior micropyle and about half contain embryos.
Eggs laid by homozygous females are ventralised, fused and shortened dorsal appendages and the eggs are longer than wild type. Expression of phlF22.hs causes females to lay ventralised eggs, expression in the anterior follicle cells of homozygotes or heterozygotes with Egfrt2 causes dorsalisation of the egg chamber.
Weak and intermediate ventralised embryos.
Homozygous adult viable.
Clonal analysis reveals phenotypes in the adult including loss of wing vein, ectopic wing vein, reduced cell size, extra bristles, cell lethality and tergite bristle abnormalities. Phenotype is cell autonomous. rl1/Df(2R)rl10a strongly enhances the wing phenotype of Egfrt1/Egfrf37.
Weak ventralization of embryos. Filzkorper are normal. Only one dorsal appendage forms. 51% of laid eggs contain embryos. Mesoderm is wider than usual and cannot be enveloped by remaining epidermis. Two ventral furrows form.
Egfrt1 homozygous females also heterozygous or homozygous for fs(1)K1013 or fs(1)K101 produce eggs with a ventralised phenotype.
Embryos from double mutants with sqdix43 are strongly dorsalised.
Does not suppress the "Ellipse" phenotype. Ommatidia are missing photoreceptor cells, and photoreceptor cell morphology may be abnormal in hemizygotes.
Reduction or absence of dorsal appendages (ventralization) with increase in main body egg shell. Micropyle at both ends of eggs, embryos ventralized.
Viable.
An enlarged mass of mesodermal cells invaginates on the ventral side of the embryo, the enlarged mesoderm is often organised into two ventral furrows. Organisation is lost in later stages of development and a mass of mesodermal cells fills the ventral half of the embryo. Respiratory appendages are fused and inserted directly on the dorsal midline.
maternal-effect lethal
Egfrt1 has visible phenotype, enhanceable by E(Egfr)B56B56
Egfrt1 has visible phenotype, enhanceable by E(Egfr)C12C12
Egfrt1 has visible phenotype, enhanceable by E(Egfr)C22C22
Egfrt1/Egfrf11 has abnormal planar polarity phenotype, suppressible | partially by fz[+]/fz21
Egfrt1/Egfrf11 has abnormal planar polarity phenotype, suppressible | partially by fz23/fz[+]
Egfr[+]/Egfrt1 is a non-enhancer of visible phenotype of Scer\GAL4en-e16E, kermitGS2053
Egfr[+]/Egfrt1 is a suppressor | partially of hyperplasia phenotype of Scer\GAL4NP5130, Src42ACA.UAS
Egfr[+]/Egfrt1 is a suppressor of increased occurrence of cell division | adult stage phenotype of BursZ5569
Egfr[+]/Egfrt1 is a suppressor of visible phenotype of Scer\GAL4Tub.PU, cswN308D.UASp
Egfrt1 is a suppressor | partially of abnormal cell polarity phenotype of aosrlt
Egfr[+]/Egfrt1 is a non-suppressor of visible phenotype of Scer\GAL4en-e16E, kermitGS2053
Egfrf3/Egfrt1 has follicle cell phenotype, enhanceable by da[+]/da2
Egfrt1 has phenotype, enhanceable by ebiunspecified/ebi[+]
Egfrt1 has wing vein L4 phenotype, enhanceable by HC21
Egfrt1 has wing vein L2 phenotype, enhanceable by E(Egfr)B56B56
Egfrt1 has wing vein L4 phenotype, enhanceable by E(Egfr)B56B56
Egfrt1 has wing vein L5 phenotype, enhanceable by E(Egfr)B56B56
Egfrt1 has wing vein L4 phenotype, enhanceable by E(Egfr)C12C12
Egfrt1 has wing vein L4 phenotype, enhanceable by E(Egfr)C22C22
Egfrt1 has anterior crossvein phenotype, enhanceable by E(Egfr)C22C22
Egfrt1 has wing vein L4 phenotype, enhanceable by groC105
Egfrt1 has anterior crossvein phenotype, enhanceable by groC105
Egfrt1 has wing vein L4 phenotype, enhanceable by vnC221
Egfrt1/Egfrtop-CA has phenotype, enhanceable by rl10b
Egfrt1/Egfrf11 has phenotype, enhanceable by rhounspecified/rhounspecified
Egfrt1/Egfrf11 has phenotype, enhanceable by vnunspecified/vnunspecified
Egfrt1/Egfrf11 has phenotype, enhanceable by ciunspecified/ciunspecified
Egfrt1/Egfrf11 has phenotype, enhanceable by astunspecified/astunspecified
Egfrt1/Egfrf11 has phenotype, enhanceable by Vnounspecified/Vno[+]
Egfrt1/Egfrf11 has phenotype, enhanceable by vvlunspecified/vvl[+]
Egfrt1 has egg phenotype, non-enhanceable by Scer\GAL4T155/Egfr::kek1UAS.cGa
Egfrt1/Egfrf11 has ommatidium phenotype, suppressible | partially by fz[+]/fz21
Egfrt1/Egfrf11 has ommatidium phenotype, suppressible | partially by fz23/fz[+]
Egfrflb-2E07/Egfrt1 has eye phenotype, suppressible by kek1RA5/kek1RM2
Df(2R)Egfr3/Egfrt1 has eye phenotype, suppressible | partially by kek1RA5/kek1[+]
Egfrflb-2E07/Egfrt1 has eye phenotype, suppressible | partially by kek1RA5/kek1[+]
Egfrflb-2E07/Egfrt1 has eye phenotype, suppressible | partially by kek1[+]/kek1RM2
Egfrt1/Egfrf24 has wing vein L4 phenotype, suppressible by kek1RA5/kek1RM2
Egfrt1/Egfrf11 has anterior crossvein phenotype, suppressible | partially by ash2[+]/ash2I1
Egfrt1/Egfrf11 has wing vein L4 phenotype, suppressible | partially by ash2[+]/ash2I1
Egfrt1 has embryonic/first instar larval cuticle phenotype, suppressible | partially by spz[+]/spzunspecified
Egfrt1 has ventral furrow | ectopic phenotype, suppressible by spz[+]/spzunspecified
Egfrt1 has ventral furrow | increased number phenotype, suppressible by spz[+]/spzunspecified
Egfrf37/Egfrt1 has wing vein L4 phenotype, suppressible by emc1/emc11
Egfrt1/Egfrf11 has wing vein L4 phenotype, suppressible by sty[+]/styS73
Egfrt1/Egfrf11 has anterior crossvein phenotype, suppressible by sty[+]/styS73
Egfrt1/Egfrf11 has wing vein L4 phenotype, suppressible by sty[+]/styS88
Egfrt1/Egfrf11 has anterior crossvein phenotype, suppressible by sty[+]/styS88
Egfrflb-2E07/Egfrt1 has dorsal appendage phenotype, suppressible | partially by kek1RA5/kek1RM2
Egfrt1/Egfrf11 has phenotype, suppressible by tkvunspecified/tkvunspecified
Egfrt1/Egfrf11 has phenotype, suppressible by netunspecified/netunspecified
Egfrt1/Egfrf11 has wing vein phenotype, suppressible by netunspecified/netunspecified
Egfrt1 has egg phenotype, non-suppressible by Scer\GAL4T155/Egfr::kek1UAS.cGa
Egfrt1 is an enhancer of ommatidium phenotype of S48-5
Egfr[+]/Egfrt1 is an enhancer of egg phenotype of Scer\GAL4VP16.nos.UTR, kek1UASp.cGa
Egfr[+]/Egfrt1 is an enhancer of crossvein | increased number phenotype of Pcyt1Ala.UAS, Scer\GAL4sd-SG29.1
Egfrt1 is an enhancer of prothoracic leg phenotype of rl1
Egfrt1 is an enhancer of prothoracic leg phenotype of gro1
Egfrt1/Egfrf11 is an enhancer of phenotype of vnunspecified
Egfrt1/Egfrf11 is an enhancer of phenotype of astunspecified
Egfrt1/Egfrf11 is an enhancer of phenotype of Vnounspecified
Egfrt1/Egfrf11 is an enhancer of phenotype of vvlunspecified
Egfrt1/Egfrf11 is an enhancer of phenotype of ciunspecified
Egfr[+]/Egfrt1 is a non-enhancer of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053
Egfr[+]/Egfrt1 is a non-enhancer of embryonic epidermis phenotype of cactE10
Egfrt1 is a non-enhancer of embryonic epidermis phenotype of fus1
Egfr[+]/Egfrt1 is a suppressor | partially of intestinal stem cell of posterior adult midgut epithelium phenotype of Scer\GAL4NP5130, Src42ACA.UAS
Egfr[+]/Egfrt1 is a suppressor of adult midgut phenotype of BursZ5569
Egfr[+]/Egfrt1 is a suppressor of intestinal stem cell | adult stage phenotype of BursZ5569
Egfrt1, Scer\GAL4Tub.PU is a suppressor of wing vein phenotype of Scer\GAL4Tub.PU, cswY279C.UASp
Egfrt1, Scer\GAL4Tub.PU is a suppressor of eye phenotype of Scer\GAL4Tub.PU, cswY279C.UASp
Egfrt1, Scer\GAL4Tub.PU is a suppressor of ommatidium phenotype of Scer\GAL4Tub.PU, cswY279C.UASp
Egfrt1, Scer\GAL4Tub.PU is a suppressor of photoreceptor cell R7 | ectopic phenotype of Scer\GAL4Tub.PU, cswY279C.UASp
Egfr[+]/Egfrt1 is a suppressor of wing vein | ectopic phenotype of Scer\GAL4Tub.PU, cswN308D.UASp
Egfr[+]/Egfrt1 is a suppressor of wing phenotype of NrgGPI.UAS, Nrgl3, Scer\GAL4MS1075
Egfr[+]/Egfrt1 is a suppressor of wing phenotype of NrgGPI.UAS, Scer\GAL4MS1075
Egfrt1 is a suppressor of wing phenotype of NrgGPI.UAS, Scer\GAL4MS1075
Egfr[+]/Egfrt1 is a suppressor of wing phenotype of Nrg167.UAS, Nrgl3, Scer\GAL4MS1075
Egfr[+]/Egfrt1 is a suppressor of wing phenotype of Nrg167.UAS, Scer\GAL4MS1075
Egfrt1 is a suppressor of wing phenotype of Nrg167.UAS, Scer\GAL4MS1075
Egfrt1 is a suppressor | partially of ommatidium phenotype of aosrlt
Egfrt1/Egfrt1 is a suppressor of egg phenotype of Egfr::kek1UAS.cGa, Scer\GAL4T155
Egfrt1/Egfrf11 is a suppressor of wing vein phenotype of netunspecified
Egfrt1/Egfrf11 is a suppressor of phenotype of tkvunspecified
Egfr[+]/Egfrt1 is a non-suppressor of wing hair phenotype of Scer\GAL4en-e16E, kermitGS2053
Egfrt1 is a non-suppressor of ommatidium phenotype of ecspok
Egfr[+]/Egfrt1 is a non-suppressor of egg chorion phenotype of Egfr::kek1KEΔCG.UAS.GFP, Scer\GAL4CY2
Egfrt1, cicfet-T6/cicfet-E11 has egg chorion phenotype
Egfrt1, cicfet-T6/cicfet-E11 has embryonic/first instar larval cuticle phenotype
Egfrt1, spzunspecified has ventral furrow phenotype
Egfrt1, spz[+]/spzunspecified has ventral furrow phenotype
Egfrt1, HC21 has ocellar bristle phenotype
Egfrt1, HC21 has anterior orbital bristle phenotype
Df(3R)Espl22, Egfrt1 has wing vein L4 phenotype
Egfrf37/Egfrt1, vvlsep/vvlsep has wing vein L2 phenotype
Egfrf37/Egfrt1, vvlsep/vvlsep has wing vein L3 phenotype
Df(2R)rl10a, Egfrt1 has wing vein L4 phenotype
The presence of a Egfrt1 background significantly suppresses ectopic wing vein formation found in cswY279C.Scer\UAS (Scer\GAL4tub) mutants.
A Egfrt1 background suppresses the cswY279C.Scer\UAS (Scer\GAL4tub) rough eye phenotype, normalizing the numbers of R7 and ommatidial rotation.
Heterozygous kek1RM2 or kek1RA5 partially suppresses the rough eye phenotype seen Egfrflb-2E07/Egfrt1 animals. kek1RM2/kek1RA5 fully suppresses the phenotype. Heterozygous kek1RA5 partially suppresses the rough eye phenotype seen Egfrf24/Egfrt1 animals. Heterozygous kek1RA5 partially suppresses the rough eye phenotype seen Df(2R)Egfr3/Egfrt1 animals.
The loss of wing vein L4 seen in Egfrt1/Egfrf24 animals is suppressed if they are also mutant for kek1 (kek1RA5/kek1RM2). The dorsalised chorion phenotype caused by expression of Egfr::kek1KEΔCG.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4CY2 is not suppressed if the flies are also heterozygous for Egfrt1.
The defective wing phenotype, seen in both Nrg167.Scer\UAS and NrgGPI.Scer\UAS mutants expressed under the control of Scer\GAL4MS1075, is partially suppressed in a Egfrt1 or Egfrt1/+ background. Egfrt1 also suppresses the wing phenotype when either Nrg167.Scer\UAS or NrgGPI.Scer\UAS is expressed in a Nrgl3 background.
The ventralized phenotype of eggs laid by Egfrt1 homozygous females is unaffected by Egfr::kek1Scer\UAS.cGa; Scer\GAL4T155. The penetrance and expressivity of the weak ventralization phenotype seen in eggs laid by kek1Scer\UAS.P\T.cGa; Scer\GAL4nos.UTR.T:Hsim\VP16 females is strongly enhanced by heterozygosity for Egfrt1 (64% of eggs are ventralized, 8% strongly ventralised).
The extra crossvein wing phenotype of Cct1Ala.Scer\UAS mutants is enhanced when Cct1Ala.Scer\UAS is overexpressed (driven by Scer\GAL4sd-SG29.1) in Egfrt1/+ flies.
Egfrt1/Egfrt1 ; cicfet-T6/cicfet-E11 females lay eggs with a dorsalised embryo (like cicfet-T6/cicfet-E11 single mutant females) and an eggshell that shows a combination of cic and Egfr mutant phenotypes; the dorsal appendages are broadened (as in cic mutants) but are positioned closer to the dorsal midline (as in Egfr mutants).
Embryos derived from Egfrt1 ; spzunspecified/+ females fail to invaginate ventral furrows and their cuticles show a reduction in ventral fates as compared to embryos laid by Egfrt1 single mutant females.
The Egfrt1/Egfrflb-2E07 phenotype is partially suppressed by kek1RA5/kek1RM2.
When Egfrf1/Egfrt1; rhove-1/rhove-1 flies are raised at the permissive temperature (18oC) a wing vein phenotype is seen that is similar to (but slightly more severe than) rhove-1/rhove-1 alone; wing veins L4 and L5 have prominent distal truncations, L2 and L3 remain largely intact. When raised at the non-permissive temperature (29oC) there is an enhancement of the wing vein phenotype with all wing veins showing major truncations. This phenotype is seen if heat shocking occurs from 0 to 24 hours APF (after puparium formation).
Egfrt1/Egfrt1; HC21/H+ flies exhibit reduced numbers of macrochaetae, most noticeably on the head. Egfrt1/Df(2R)Egfr18; gro1/gro1 flies display a combination of Egfr and gro phenotypes. The L4 wing vein defect is restored and the ocellar bristles are present but disarrayed. In many cases supernumerary ocellar bristles and/or interocellar bristles are present producing a patch of bristles where the ocelli would normally have been. The ocelli are either reduced or eliminated. Most males display one or more ectopic eye structure dorso-medial to the normal eyes. These structures are small and contain discrete ommatidia but no interommatidial bristles. The lens material of these ectopic eyes is fused into a uniform glaze and they are recessed into the head cuticle.
Weak suppression of the dosage-dependent (two or more copies of P{sev-svp1} or P{sev-svp2}) transformation of cone cells into R7 photoreceptors and at a lower frequency R7 cells into outer photoreceptors.
rl10b causes strong dominant enhancement of the Egfrtop-CA/Egfrt1 transheterozygous phenotype.
Double mutants of Df(2R)rl10a and Egfrt1 show disruption of the longitudinal vein L4.
Double mutant combinations of Egfrt1/Egfrf11 with rhounspecified/rhounspecified, vnunspecified/vnunspecified, ciunspecified/ciunspecified, astunspecified/astunspecified, tt1/tt1, Vnounspecified/+, H2/+, vvlunspecified/+ or NAx-M1/+ have a superadditive phenotype. Double mutant combinations of Egfrt1/Egfrf11 with kn1/kn1 or netunspecified/netunspecified show mutual suppression, with both the Egfr and vein phenotypes being normalised. Egfrt1/Egfrf11 shows a negative interaction in combination with either DlM1/+ or tkvunspecified/tkvunspecified. Egfrt1/Egfrf11 shows a simple additive phenotype with dppunspecified/dppunspecified, kniunspecified/kniunspecified, tg2/tg2, cv-2unspecified/cv-2unspecified and N55e11/+. E(spl)rv1 grounspecified Egfrt1/Egfrf11 flies show a simple additive phenotype.
Weak Egfr allele.
Mutation of Egfr that coordinately affects all gene activities, a class I lesion. The allelic series for class I lesions: Egfrt2 = Egfrt1 < Egfrtop-EC20 < Egfrf7 = Egfrf1 = Egfrflb-2E07 < Egfrtop-EE39 = Egfrtop-ED26 = Egfrf5 < Egfrf9 = Egfrf10 = Egfrf2 = Egfrtop-EE42 = Egfrf11 = Egfrf24 = Egfrf3 = Df(2R)Egfr3.
Class I allele.