Allele Dmel\Egfr^t2

General Information
Symbol	Dmel\Egfr^t2	Species	D. melanogaster
Name		FlyBase ID	FBal0003573
Feature type	allele	Associated gene	Dmel\Egfr
Also Known As	top^CJ, egfr^CJ

Map ( GBrowse )
Allele class
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	ethyl methanesulfonate (Clifford and Schupbach, 1989, Clifford and Schupbach, 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:17,442,353..17,442,353 evidence=experimental na_change=C17442353T pr_change=S58F\|Egfr-PA,S107F\|Egfr-PB reported_na_change=C406T reported_pr_change=S58F (Clifford and Schupbach, 1994)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: C406T. Amino acid replacement: S58F. (Clifford and Schupbach, 1994)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Price et al., 1989) female sterile \| recessive (Clifford and Schupbach, 1989) lethal \| embryonic stage \| maternal effect (Roth and Schupbach, 1994) viable (Baker and Rubin, 1992)
Phenotype Manifest In
	dorsal appendage \| maternal effect (Roth and Schupbach, 1994) ventral furrow \| maternal effect (Roth and Schupbach, 1994) wing vein L4 (with Egfr^f3) (Freeman, 1996)
Detailed Description
	Statement Reference Dsor1 mutant alleles do not suppress the eggshell dorso-ventral polarity defect of the Egfr^t2 maternal mutation. (Lim et al., 1997) Egfr^f3/Egfr^t2 wings have a gap in L4 wing vein. (Freeman, 1996) Transheterozygous females with Egfr^f2 display egg chambers with gaps in the follicular epithelium that uncover the nurse cells. Eggs derived from these females display weak or intermediate ventralised phenotypes. (Goode et al., 1996) Expression of phl^F22.hs in the anterior follicle cells of heterozygotes with Egfr^t1 causes dorsalisation of the egg chamber. (Brand and Perrimon, 1994) Homozygous adult viable. (Clifford and Schupbach, 1994) Weak ventralization of embryos. Filzkorper are normal. Only one dorsal appendage forms. 51% of laid eggs contain embryos. Mesoderm is wider than usual and cannot be enveloped by remaining epidermis. Two ventral furrows form. (Roth and Schupbach, 1994) Does not suppress the "Ellipse" phenotype. Hemizygotes die during pupariation, and have abnormally small dorsal imaginal discs. (Baker and Rubin, 1992) Viable as a homozygote and hemizygote. Adults exhibit milder eye defects than would be expected from the severity of the wing and haltere defects. (Clifford and Schupbach, 1989) maternal-effect lethal
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Suppressed by
Statement Reference Egfr^f3/Egfr^t2 has wing vein L4 phenotype, suppressible by sev::tor^13D.hs.sev (Freeman, 1996)
Other
Statement Reference Egfr^t2, hup¹ has egg chamber phenotype (Rotoli et al., 1998, Rotoli et al., 1998) Egfr^t2, hup¹ has follicle cell phenotype (Rotoli et al., 1998, Rotoli et al., 1998) Egfr^t2, hup¹ has nurse cell phenotype (Rotoli et al., 1998, Rotoli et al., 1998)
Additional Comments
Genetic Interactions
Statement Reference 16% of Egfr^t2 hup¹ double homozygous egg chambers are dicephalic, and an additional 12.5% of double homozygous egg chambers (either dicephalic or with the oocyte normally localised) show degenerated nurse cells. 10% of double homozygous egg chambers have multiple layers of follicle cells at the posterior end. In some cases, these follicle cells try to migrate centripetally at the posterior, splitting the cytoplasm of the oocyte. (Rotoli et al., 1998) Egfr^f3/Egfr^t2 vein gap can be rescued by one copy of sev::tor^13D.hs.sev. Ectopic expression also causes extra vein phenotype, notably around the distal end of L2 and broadening or deltas at the distal tips of most of the veins. (Freeman, 1996)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Class I allele. (Clifford and Schupbach, 1994) Mutation of Egfr that coordinately affects all gene activities, a class I lesion. The allelic series for class I lesions: Egfr^t2 = Egfr^t1 < Egfr^top-EC20 < Egfr^f7 = Egfr^f1 = Egfr^flb-2E07 < Egfr^top-EE39 = Egfr^top-ED26 = Egfr^f5 < Egfr^f9 = Egfr^f10 = Egfr^f2 = Egfr^top-EE42 = Egfr^f11 = Egfr^f24 = Egfr^f3 = Df(2R)Egfr3. (Clifford and Schupbach, 1989)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 6 )
Reported As
Symbol Synonym	egfr^CJ (Ghiglione et al., 1999) Egfr^CJ (Neuman-Silberberg and Schupbach, 1996) Egf-r^CJ (Sturtevant et al., 1993) Egfr^t2 top^CJ (Grammont et al., 2000, Rotoli et al., 1998, Sapir et al., 1998, Lim et al., 1997, Goode et al., 1996, Brand and Perrimon, 1994, Roth and Schupbach, 1994, Clifford and Schupbach, 1994, Baker and Rubin, 1992, Clifford and Schupbach, 1989, Price et al., 1989, ) top^cj (Freeman, 1996)
Name Synonym
Secondary FlyBase IDs

References ( 17 )
Research paper	Grammont et al., 2000, Mech. Dev. 90(2): 289--292 Expression and cellular localization of the Toucan protein during Drosophila oogenesis. [FBrf0125267] Ghiglione et al., 1999, Cell 96(6): 847--856 The transmembrane molecule kekkon 1 acts in a feedback loop to negatively regulate the activity of the Drosophila EGF receptor during oogenesis. [FBrf0107731] Rotoli et al., 1998, Genetics 148(2): 767--773 hold up is required for establishment of oocyte positioning, follicle cell fate and egg polarity and cooperates with Egfr during Drosophila oogenesis. [FBrf0101997] Sapir et al., 1998, Development 125(2): 191--200 Sequential activation of the EGF receptor pathway during Drosophila oogenesis establishes the dorsoventral axis. [FBrf0100630] Lim et al., 1997, Genetics 146(1): 263--273 Dominant mutations of Drosophila MAP kinase kinase and their activities in Drosophila and yeast MAP kinase cascades. [FBrf0093573] Freeman, 1996, Cell 87(4): 651--660 Reiterative use of the EGF receptor triggers differentiation of all cell types in the Drosophila eye. [FBrf0090558] Goode et al., 1996, Dev. Biol. 178(1): 35--50 brainiac encodes a novel, putative secreted protein that cooperates with Grk TGF in the genesis of the follicular epithelium. [FBrf0089663] Neuman-Silberberg and Schupbach, 1996, Mech. Dev. 59(2): 105--113 The Drosophila TGF--like protein Gurken: expression and cellular localization during Drosophila oogenesis. [FBrf0090712] Brand and Perrimon, 1994, Genes Dev. 8(5): 629--639 Raf acts downstream of the EGF receptor to determine dorsoventral polarity during Drosophila oogenesis. [FBrf0076497] Clifford and Schupbach, 1994, Genetics 137(2): 531--550 Molecular analysis of the Drosophila EGF receptor homolog reveals that several genetically defined classes of alleles cluster in subdomains of the receptor protein. [FBrf0072848] Roth and Schupbach, 1994, Development 120(8): 2245--2257 The relationship between ovarian and embryonic dorsoventral patterning in Drosophila. [FBrf0075066] Sturtevant et al., 1993, Genes Dev. 7(6): 961--973 The Drosophila rhomboid gene mediates the localized formation of wing veins and interacts genetically with components of the EGF-R signaling pathway. [FBrf0058540] Baker and Rubin, 1992, Dev. Biol. 150: 381--396 Ellipse mutations in the Drosophila homologue of the EGF receptor affect pattern formation, cell division, and cell death in eye imaginal discs. [FBrf0055813] Goode et al., 1992, Development 116(1): 177--192 The neurogenic locus brainiac cooperates with the Drosophila EGF receptor to establish the ovarian follicle and to determine its dorsal-ventral polarity. [FBrf0055851] Clifford and Schupbach, 1989, Genetics 123: 771--787 Coordinately and differentially mutable activities of torpedo, the Drosophila melanogaster homolog of the vertebrate EGF receptor gene. [FBrf0049928] Price et al., 1989, Cell 56: 1085--1092 The maternal ventralizing locus torpedo is allelic to faint little ball, an embryonic lethal, and encodes the Drosophila EGF receptor homolog. [FBrf0049331] Schupbach, 1987, Cell 49: 699--707 Germ line and soma cooperate during oogenesis to establish the dorsoventral pattern of egg shell and embryo in Drosophila melanogaster. [FBrf0045776]

Allele Dmel\Egfrt2

Allele Dmel\Egfr^t2