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General Information
Symbol
Dmel\egl4
Species
D. melanogaster
Name
FlyBase ID
FBal0003577
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
eglPR29
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C23737969T

Amino acid change:

R290term | egl-PB; R290term | egl-PC

Reported amino acid change:

R279term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. The annotated egl protein begins at an AUG 11aa upstream of the reported protein accounting for the difference in the curated and reported mutation positions.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: R279term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

egl1/egl4 zygotic mutant larvae display normal neuromuscular junction (NMJ) morphology and muscle size, and their locomotion appears normal.

egl1/egl4 third instar larvae have no defects in the localisation of nuclei in the optic stalk or retina.

Embryos derived from egl3e/egl1 mothers mated to egl4/+ fathers often show a disrupted epidermis. At stage 13, the salivary gland placode area is often disrupted. Salivary gland morphogenesis is disrupted in that cells of the placode often do not change their apices in a coordinated way; the invagination hole appears too large and extended; and the invaginated portion of the glands often has an irregular shape.

All 16 cells of developing egl1/egl4 mutant ovaries initially enter mitosis and form a synaptonemal complex. The synaptonemal complex dissolves in later egg chambers and all 16 cells become polyploid nurse cells.

16 cell vitellarial cysts cease to enlarge partway down the vitellarium and are eventually phagocytosed. Cyst production dynamics are normal. All 16 nuclei in a cyst enter meiosis; the chromatin condenses and synaptonemal complex is present. Later all 16 lose the synaptonemal complex and assume nurse cell characteristics. Cell shape changes within the cyst are normal as the cysts pass down the germarium. Synaptonemal complex is of normal width and is apparently normal in substructure, but throughout pachytene it maintains the extreme thinness typical of wild-type synaptonemal complex during zygotene. Most "pachytene" nuclei in egl mutant cysts have zygotene numbers of synaptonemal complex segments as well as zygotene thinness. The nuclei do mount both early and late recombination nodules, at normal positions within the germarium. Number of recombination nodules per nucleus is comparable to that in wild type pro-oocyte. The onset of cytoplasmic flow is delayed. In mid-vitellarium the 16 cells remain the same size. Ring canals frequently do not enlarge as much as they should, and are more likely to be lacking their inner lining than in wild type. The fusome is apparently normal. Centrioles and mitochondria orient normally. Centriole replication continues. Mitochondria indicate that the ring canals may be unusually difficult to pass through.

Egg chambers contain 16 nurse cells and no oocyte, many egg chambers are degenerating.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

When transheterozygous with a deficiency, ovary size is reduced compared to the egl4 homozygote.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (11)