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General Information
Symbol
Dmel\flw1
Species
D. melanogaster
Name
FlyBase ID
FBal0004118
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
PP1β9C1
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    point mutation
    Nucleotide change:

    T10407272C

    Reported nucleotide change:

    T?C

    Amino acid change:

    V415A | flw-PA; V284A | flw-PB; V284A | flw-PC; V284A | flw-PD

    Reported amino acid change:

    V284A

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Amino acid replacement: V284A. Nucleotide substitution: GTG?GCG

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    Hemizygous males have wild-type wings.

    flw1 mutant follicle cell clones generate an irregular wavy egg chamber epithelium. Mutant egg chambers are not round or ellipsoid like in wild-type but are either stretched or develop bulges.

    flw1 hemizygotes have normal wings.

    flw1/flw6 flies are completely flightless. flw1 flies show a variable indirect flight muscle (IFM) phenotype ranging from normal through disorganised to absent IFMs. IFMs are never present in flw1/Df(1)N110, flw1/Df(1)Hk or flw1/flw6 flies, but the jump muscle (tergal depressor of trochanter muscle) appears normal. The early development of IFMs in flw1/Df(1)Hk pupae appears normal the pretemplates on which the dorsal longitudinal muscles form are present, the dorsoventral muscles also develop normally. Muscle defects become apparent at about 28 hours after puparium formation. Most of the muscles break away from their posterior attachment sites and are found as ball-like structures at the anterior segment boundaries, the sarcomere organisation in these balls appears normal.

    DLM and DVMs are scant. Myofibrils are disorganized, with variable fiber diameter, and Z bands are crooked.

    Hemizygotes have bubbles beneath the thoracic cuticle and held up wings.

    RK2.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    NOT Enhanced by
    Statement
    Reference

    flw1 has viable phenotype, non-enhanceable by Mbs3

    flw1 has viable phenotype, non-enhanceable by sqhA20.Tag:FLAG

    Suppressed by
    Statement
    Reference

    flw1, pucA251.1F3/puc[+] has visible phenotype, suppressible by bsk1/bsk[+]

    flw1, pucA251.1F3/puc[+] has visible phenotype, suppressible by zip[+]/zip1

    flw1, pucA251.1F3/puc[+] has visible phenotype, suppressible by sqhE20.E21

    NOT suppressed by
    Statement
    Reference

    flw1, pucA251.1F3/puc[+] has visible phenotype, non-suppressible by sqhA20.A21.Tag:FLAG

    Suppressor of
    Statement
    Reference
    NOT Suppressor of
    Other
    Statement
    Reference

    Mbs3/Mbs[+], flw1, pucA251.1F3/puc[+] has lethal phenotype

    Mbs3/Mbs[+], flw1 has viable phenotype

    Pp1α-96AGS11179/Pp1α-96A[+], flw1 has viable phenotype

    Pp1-87B87Bg-3/Pp1-87B[+], flw1 has viable phenotype

    Phenotype Manifest In
    Suppressed by
    Statement
    Reference

    flw1, pucA251.1F3/puc[+] has wing phenotype, suppressible by bsk1/bsk[+]

    flw1, pucA251.1F3/puc[+] has wing phenotype, suppressible by zip[+]/zip1

    flw1, pucA251.1F3/puc[+] has wing phenotype, suppressible by sqhE20.E21

    NOT suppressed by
    Statement
    Reference

    flw1, pucA251.1F3/puc[+] has wing phenotype, non-suppressible by sqhA20.A21.Tag:FLAG

    flw1 has phenotype, non-suppressible by Mlc2S66A,S67A

    Enhancer of
    NOT Enhancer of
    Statement
    Reference

    flw[+]/flw1 is a non-enhancer of egg | germline clone phenotype of Pkn1T

    flw[+]/flw1 is a non-enhancer of egg | germline clone phenotype of Pkn2T

    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    flw1/+ does not enhance the shorter egg length phenotype seen in Pkn1T/Pkn1T or Pkn2T/Pkn2T germline clones.

    The flw1/Y ; puc67BDn/+ combination is semi-lethal (90% of pupae fail to eclose) and surviving males have strong wing defects and occasional third leg malformations.

    The flw1/Y ; pucA251.1F3/+ combination is viable. 30% of these flies have wing phenotypes that range from slight defects in the posterior part of the wing to stumps. Flies with the most severe wing phenotypes sometimes have third leg malformations.

    The wing defects caused by expression of hepCA.Scer\UAS under the control of Scer\GAL4vg.PU are enhanced in a flw1/Y background; the wings are much smaller and are frequently notched and blistered. The double mutant flies are only 10% viable relative to their flw1/+ ; hepCA.Scer\UAS, Scer\GAL4vg.PU control siblings.

    The wing defects seen in flw1/Y ; pucA251.1F3/+ flies are suppressed to wild type by bsk1/+, zip1/+ or sqhE20.E21.

    The wing defects seen in flw1/Y ; pucA251.1F3/+ flies are not suppressed by sqhA20.A21.T:Zzzz\FLAG.

    Expression of Nop17lScer\UAS.T:Zzzz\His6,T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 in hemizygous flw1 mutant wings results in severely crumpled and reduced wings.

    Expression of Nop17lScer\UAS.T:Zzzz\His6,T:Hsap\MYC under the control of Scer\GAL4arm.PS is synthetic lethal in a hemizygous flw1 mutant background.

    The synthetic lethality resulting from the overexpression of Nop17lScer\UAS.T:Zzzz\His6,T:Hsap\MYC under the control of Scer\GAL4arm.PS in a hemizygous flw1 mutant background can be rescued by the co-expression of flwScer\UAS.T:Ivir\HA1.

    The flw1 mutation is lethal in combination with the phosphomimetic sqhE20.E21 mutation. Most flw1/Y; sqhE21.T:Zzzz\FLAG/+ flies are viable, but have a crumpled wing phenotype, resembling that of stronger flw alleles. The Mbs3 mutation does not affect the viability of flw1 mutants.

    The addition of Mlc2S66A,S67A does not suppress flw mutant phenotypes.

    Xenogenetic Interactions
    Statement
    Reference
    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (3)
    Notes on Origin
    Discoverer

    Waletzky, 1937.

    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (3)
    References (12)