Allele Dmel\spn-E^hls-03987

General Information
Symbol	Dmel\spn-E^hls-03987	Species	D. melanogaster
Name		FlyBase ID	FBal0004296
Feature type	allele	Associated gene	Dmel\spn-E
Also Known As	spn-E^hls3987, hls³⁹⁸⁷

Map ( GBrowse )	Untitled Document
Allele class	hypomorphic allele - genetic evidence
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Gillespie and Berg, 1995)
Mutagen	P-element activity (Gillespie and Berg, 1994, Gillespie and Berg, 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 3R:11,663,317..11,663,317 3R:11,663,324..11,663,324 (Gillespie and Berg, 1994, BDGP Project Members, 1994-1999, Gillespie and Berg, 1995, Spradling et al., 1999, Rouget et al., 2010)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference P{PZ} insertion within the coding sequences. (Gillespie and Berg, 1995)
Caused by insertion	P{PZ}spn-E^hls-03987 (Gillespie and Berg, 1994, BDGP Project Members, 1994-1999, Gillespie and Berg, 1995, Spradling et al., 1999, Rouget et al., 2010)
Cytology
Phenotypic Data
Phenotypic Class
	female sterile (Gillespie and Berg, 1994, Gillespie and Berg, 1995, Berg, 1994) female sterile \| recessive (Spradling et al., 1999) meiotic cell cycle defective (Stapleton et al., 2001) meiotic cell cycle defective (with spn-E¹) (Stapleton et al., 2001) sterile (with spn-E^hls-Δ139) (Gillespie and Berg, 1995) sterile (with spn-E^hls-Δ157) (Gillespie and Berg, 1995) suppressor of variegation (Pal-Bhadra et al., 2004)
Phenotype Manifest In
	dorsal appendage (Gillespie and Berg, 1994) egg chamber (Gillespie and Berg, 1994, Gillespie and Berg, 1995) meiosis & nuclear chromosome (Stapleton et al., 2001) meiosis & nuclear chromosome (with spn-E¹) (Stapleton et al., 2001) microtubule (Gillespie and Berg, 1995) oocyte (Gillespie and Berg, 1994, Gillespie and Berg, 1995) P granule (with spn-E¹) (Snee and Macdonald, 2004)
Detailed Description
	Statement Reference Suppressor of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon. (Pal-Bhadra et al., 2004) Nuage particles are lost in spn-E¹/spn-E^hls-03987 nurse cells and the perinuclear nuage is smoother than normal. (Snee and Macdonald, 2004) Homozygotes and trans-heterozygotes with spn-E¹ show an increased rate of non-disjunction in all except the small 4th chromosome. There are also substantial biases in recovery of reciprocal sperm classes. Nullo-XY sperm are recovered in considerable excess over the reciprocal XY sperm class. Also a weaker bias in favour of normal X-bearing sperm is seen. (Stapleton et al., 2001) Females lay few eggs and they appear to be unfertilised. Oocyte is not located at the usual posterior position within the egg, 95% of late stage egg chambers were ventralised and a small number of the egg chambers are degenerating. Heterozygotes with Df(3R)sbd105 (spn-E^-) exhibit mislocalised oocyte phenotype and degenerating egg chambers. Microtubule structure is disrupted in the egg chambers due to the inappropriate formation of an extensive microtubule meshwork during stage 8 to 9 (not premature cytoplasmic streaming). (Gillespie and Berg, 1995) 10% of eggs fail to localise the oocyte to the posterior of the egg chamber, 95% eggs have variable fused filaments. (Berg, 1994) Affects the early anteroposterior process in oogenesis. 5-10% of stage 2 to 10 eggs produced by homozygous female have mislocalised oocytes. 95% late stage egg chambers have a ventralised phenotype with only one respiratory appendage on the dorsal midline or two shortened appendages. No eggs hatch. (Gillespie and Berg, 1994) female-sterile 10% of eggs fail to localize oocyte to posterior of egg chamber; 95% of eggs have variable fused filaments.
External Data
Linkouts
Interactions
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement Reference
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 1 )
Bloomington	24853 mwh¹ ry⁵⁰⁶ P{PZ}spn-E^hls-03987 e^s/TM3, ry^RK Sb¹
Notes on Origin
Discoverer

Comments
	Excision of the P{PZ} element produces a fully fertile phenotype demonstrating the insertion to be responsible for the female sterility. (Gillespie and Berg, 1995)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 11 )
Reported As
Symbol Synonym	fs(3)03987 (Spradling et al., 1999) fs(3)hls¹ fs(3)hls³⁹⁸⁷ fs(3)hls⁰³⁹⁸⁷ hls³⁹⁸⁷ (Stapleton et al., 2001, Matsubayashi and Yamamoto, 1998, Gonzalez-Reyes et al., 1997) hls^P{lacZ}3987 (Pal-Bhadra et al., 2004) spnE³⁹⁸⁷ (Snee and Macdonald, 2004) spn-E^hls3987 (Savitsky et al., 2006, Findley et al., 2003, Gonzalez-Reyes et al., 1997, Lim and Kai, 2007, Shpiz et al., 2011, Lim and Kai, 2007, Shpiz et al., 2009, Lim et al., 2009, Patil and Kai, 2010) spn-E^hls-03987 (Usakin et al., 2007, Rouget et al., 2010) spnE^hls3987 (Anand and Kai, 2012) unnamed (Gillespie and Berg, 1994)
Name Synonym
Secondary FlyBase IDs

References ( 22 )

Generate a list of	All references relating to this allele ( 22 )

List References by type	Research paper ( 18 ) Erratum ( 1 ) Personal communication to FlyBase ( 2 ) Abstract ( 1 )
Recent research papers ( 3 )
	Anand and Kai, 2012, EMBO J. 31(4): 870--882 The tudor domain protein Kumo is required to assemble the nuage and to generate germline piRNAs in Drosophila. [FBrf0217541] Klenov et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(46): 18760--18765 Separation of stem cell maintenance and transposon silencing functions of Piwi protein. [FBrf0216776] Shpiz et al., 2011, Nucleic Acids Res. 39(20): 8703--8711 Mechanism of the piRNA-mediated silencing of Drosophila telomeric retrotransposons. [FBrf0216525] Show all research papers ...

Allele Dmel\spn-Ehls-03987

Allele Dmel\spn-E^hls-03987