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General Information
Symbol
Dmel\spn-Ehls-03987
Species
D. melanogaster
Name
FlyBase ID
FBal0004296
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
spn-Ehls3987, hls3987
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{PZ} insertion within the coding sequences.

Insertion components
P{PZ}spn-Ehls-03987
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Suppressor of repeat-induced silencing, as seen at heterochromatic tandem arrays of insertions of the P{lacW} transposon.

Nuage particles are lost in spn-E1/spn-Ehls-03987 nurse cells and the perinuclear nuage is smoother than normal.

Homozygotes and trans-heterozygotes with spn-E1 show an increased rate of non-disjunction in all except the small 4th chromosome. There are also substantial biases in recovery of reciprocal sperm classes. Nullo-XY sperm are recovered in considerable excess over the reciprocal XY sperm class. Also a weaker bias in favour of normal X-bearing sperm is seen.

Females lay few eggs and they appear to be unfertilised. Oocyte is not located at the usual posterior position within the egg, 95% of late stage egg chambers were ventralised and a small number of the egg chambers are degenerating. Heterozygotes with Df(3R)sbd105 (spn-E-) exhibit mislocalised oocyte phenotype and degenerating egg chambers. Microtubule structure is disrupted in the egg chambers due to the inappropriate formation of an extensive microtubule meshwork during stage 8 to 9 (not premature cytoplasmic streaming).

10% of eggs fail to localise the oocyte to the posterior of the egg chamber, 95% eggs have variable fused filaments.

Affects the early anteroposterior process in oogenesis. 5-10% of stage 2 to 10 eggs produced by homozygous female have mislocalised oocytes. 95% late stage egg chambers have a ventralised phenotype with only one respiratory appendage on the dorsal midline or two shortened appendages. No eggs hatch.

female-sterile 10% of eggs fail to localize oocyte to posterior of egg chamber; 95% of eggs have variable fused filaments.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Excision of the P{PZ} element produces a fully fertile phenotype demonstrating the insertion to be responsible for the female sterility.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (11)
References (28)